Influence of four soil maintenance practices on Collembola communities in a Mediterranean vineyard   总被引：1，自引：0，他引：1  

Anglique Renaud  Nicole Poinsot-Balaguer  Jrme Cortet  Jean Le Petit 《Pedobiologia》2004,48(5-6):623-630

We studied the influence of four soil maintenance practices on Collembola communities in the soil of a Mediterranean vineyard: (a) postemergence herbicide with glyphosate; (b) postemergence and pre-emergence herbicides with glyphosate, terbuthylazine, diuron and oryzalin; (c) natural flora and (d) tillage to a depth of 10–15 cm. Total Collembola abundance, species diversity and species richness significantly varied between the four practices. Notably, the practice using postemergence and pre-emergence herbicides had significantly lower values. Identification of Collembola at species level allowed an interspecies comparison and revealed significant differences for the most common species between the four practices, with each practice being characterized by a different set of species. None of the species were found to be significantly more abundant in the plots treated with postemergence and pre-emergence herbicides.  相似文献   

内蒙古红豆草黄萎病菌的分离鉴定及其生物学特性     

刘宇萱  王乐  张园园  徐林波  赵君 《中国草地学报》2021,(3)

依照柯赫氏法则,利用形态学结合分子生物学的方法首次对内蒙古自治区呼和浩特市中国农科院草原研究所沙尔沁基地红豆草疑似黄萎病的病样进行分离和鉴定,利用蘸根接种法对病原菌的致病性进行测定。同时,首次在室内条件下对病菌的生物学特性,如生长最适温度、酸碱度以及碳源、氮源对病原菌生长速率和产孢量的影响进行了研究。结果表明,引起沙尔沁基地红豆草黄萎病的病原菌为大丽轮枝孢菌,菌株具有较宽的温度和酸碱度适应范围,最适于菌株生长和产孢的温度均为25℃,最适于菌株生长和产孢的pH值范围分别为7~10和5~6。在碳源选择上,大丽轮枝孢菌在可溶性淀粉上的生长速度最快,而蔗糖最有利于其产孢。在氮源选择上,大丽轮枝孢菌在牛肉膏和L-脯氨酸上的生长速度较快,且L-脯氨酸最有利于其产孢。  相似文献   

Production and Identification of Clone Sheep Using Bone Marrow Mesenchymal Stem Cells as Donor Cell     

GUO Yuan  LIU Zong-zheng  LIU Le  MENG Fan-hua  ZHANG Yan-ru  ZHOU Huan-min  LIU Chun-xia 《中国畜牧兽医》2016,43(11):3011-3018

In order to obtain the cloned sheep using bone marrow mesenchymal stem cells（BMSCs）as the donor cells,the BMSCs of sheep were chosen and reconstructed embryos were built to transfer.10 published microsatellite markers were chosen,and the DNA samples from clone sheep,donor cells and surrogate ewes were amplified,and the relationship of father-son（RCP）was analyzed using the Quantity One for genotyping.The results showed that the reconstructed embryos were successfully built for electric fusion using sheep BMSCs as nuclear donor,and making nuclear transplantation into enucleated mature oocytes of which the fusion rate was 80.62%.20 surrogate ewe were chosen to be implanted with the reconstructed embryos at morula stage by implant surgery,and 5 lambs were born and only 3 were survived.The genotype of cloned sheep was in line with the dornor cell and the RCP were more than 99.999%.In conclusion,the first clone sheep were obtained successfully by using BMSCs as a nuclear donor in this experiment.  相似文献   

Synthesis,insecticidal activity,and ensembled docking of nitroguanidines bearing S- and R-proline     

Alfredo Rodríguez-Hernández  Israel Bonilla-Landa  Abraham Vidal-Limon  Arturo Ibarra-Juárez  Felipe Barrera-Méndez  Francisco Javier Enríquez Medrano  Ramón E. Díaz de León  José Luis Olivares-Romero 《Pest management science》2023,79(5):1912-1921

  相似文献   

Water Pricing and Sustainability of Self-Governing Irrigation Schemes     

Pierre-Yves Le Gal  Thierry Rieu  Charles Fall 《Irrigation and Drainage Systems》2003,17(3):213-238

The sustainability of self-governingirrigation schemes is currently underpressure in many countries as publicfinancial support is decreasing.Furthermore, growing global concernregarding water scarcity means thatefficient water use is essential.Theoretically the choice and implementationof a water-pricing system should play acentral role in achieving this objective,both by recovering water costs and byencouraging farmers to adopt more efficientbehaviour. In the Senegal River Delta newlyestablished water users associations (WUAs)have chosen low water charges, which areaffordable for the majority of farmers butwhich underestimate long-term maintenancecosts. Combined with the difficulty ofcollecting and managing farmers' fees, thischoice has drawn them into a vicious circleleading to scheme deterioration and poorwater service. New alternatives have beendiscussed with them using a simulation toolthat takes into account both the watercosts and the farmers' incomes. Comparedwith the present fixed water charge basedonly on cultivated land, a two-part optionbased both on equipped and irrigated areasensures the recovery of fixed expenses suchas maintenance, while encouraging farmersto intensify their farming systems byadopting double cropping. Neverthelesschoosing a relevant water charge does notensure by itself the sustainability of anirrigation scheme. Combined supportproviding WUAS and farmers with efficientadvice regarding management andorganisational skills should alsocontribute significantly to achieve thisprime objective.  相似文献   

“上膜下秸”措施对温室土壤水热盐调控试验研究     

王乐  张娜  杜斌  张红玲 《中国农村水利水电》2020,(5):1-3,9

为了明确温室生产中不同耕作措施的水热盐调控效果,采用温室小区试验方法,以翻耕(CK)、翻耕结合地表覆盖地膜(M)、翻耕结合秸秆深埋(J)为对照,研究了翻耕结合地表覆盖地膜结合秸秆深埋(J+M)措施对温室土壤水热盐动态变化的影响。结果表明,与M、J及CK处理相比,J+M处理全生育期平均土壤含水率可分别提高8.96%、5.45%、14.32%,土壤温度分别提高3.35%、5.21%及8.32%,土壤全盐量分别降低24.44%、19.65%、26.64%,这一结果可为宁夏温室种植实施地膜覆盖与秸秆深埋改良措施提供参考。  相似文献   

用于池塘养殖尾水处理的串联式旋流器水动力特性及分离效率     

张俊  刘奥胜  李乐  曹守启 《农业工程学报》2022,38(20):49-58

为了将多级旋流分离技术应用于池塘养殖尾水处理,并解决旋流器的水力停留时间短、分离效率低等问题,根据池塘养殖南美白对虾的尾水特点,结合工业标准150旋流分离器的结构参数,设计了一种串联式旋流分离器。该研究基于欧拉-拉格朗日方法,结合雷诺应力湍流模型与离散相模型,建立了串联式旋流分离器的三维两相内流场数值计算模型。在对比试验数据验证计算方法有效性和流场网格独立性的基础上,分析了进水口流量、锥角、颗粒粒径和密度等参数对旋流器的流场特征和分离效率的影响机制,并提出了多级“旋流+过滤”组合式池塘养殖尾水固液分离技术。研究表明：随着进水口流量在一定范围内增加,两级旋流器内流场的切向速度、轴向速度、分流比、压力降均增大,涡流强度增强,对于粒径为75μm颗粒物的分离效率明显提高,而对于粒径为5μm颗粒物的分离效率变化不显著;随着旋流器的锥角减小,水流阻力、压力降和分流比均降低,有助于增加颗粒物在旋流区的停留时间,从而提高其分离效率;随着颗粒物的密度和粒径增大,旋流器的分离效率提高,对粒径为75μm沉淀物的分离效率可达80%,对同粒径悬浮物的分离效率约为60%,但对于粒径为5μm的2类颗粒物而言,分离效...  相似文献   

苹果外部缺陷全表面在线检测分选装置研发     

彭彦昆  孙晨  刘乐  李阳 《农业工程学报》2022,38(23):266-275

中国是水果消费大国,但在水果产后检测装备方面相对滞后。针对目前在线检测装置无法采集苹果全表面图像信息且无法精确计算缺陷面积的问题,该研究以表面缺陷面积的快速检测为主要目标,提出苹果全表面图像合成算法,设计了一套苹果外部品质在线检测及分级装置。该研究以苹果为例,基于球模型提出苹果全表面图像合成算法、缺陷面积校正算法精确计算苹果的表面缺陷面积。通过试验验证,对苹果表面图像进行分割合成后,整体的图像的漏检率为0。提出缺陷面积校正算法,可以计算图像中位于任意位置的苹果缺陷真实面积,选取了120个样本进行验证,其中擦伤样本、碰伤样本、痘斑病样本、表面腐败样本各30个。4种表面损伤面积的预测值和真实值的决定系数R²均在0.97以上,均方根误差(Root Mean Squared Error, RMSE)在4 mm²以下。在偏角试验中,4种表面损伤面积的预测值和真实值的决定系数R²均在0.974 2以上,RMSE在6.304 4 mm²以下。装置检测苹果的速度为2个/s,评级准确率为95%。研究结果表明,检测与苹...  相似文献   

淫羊藿对肾阳虚雄性大鼠肾脏BMP-7表达的影响     

周乐  崔燎  吴铁 《湛江医学院学报》2007,25(4):371-374,386

目的 观察淫羊藿对肾阳虚雄性SD大鼠肾脏骨形态发生蛋白-7(BMP-7)表达的影响.方法 3月龄雄性SD大鼠24只,随机分为正常组、肾阳虚模型组、淫羊藿组,灌胃给药90d后,RT-PCR检测肾脏BMP-7 mRNA表达,免疫组化检测肾脏BMP-7的表达.结果 正常组和淫羊藿组BMP-7mRNA和蛋白显著高于肾阳虚模型组(灰度比值:0.990±0.103和1.054:t:0.097vs0.619±0.106,P＜0.01;免疫组化:0、1、0、4和0、0、0、4vs1、2、2、0,P＜0.01).结论 淫羊藿可提高肾脏中BMP-7的表达而保护肾脏.  相似文献   

基于简化基因组测序技术的甘薯HRM分子标记开发及其应用     

冯俊彦  郎涛  张聪  李明  青莉芳  屈会娟  蒲志刚  康乐 《核农学报》2022,36(7):1350-1361

目前甘薯中针对SNP位点的分子标记开发及应用的报道较少。为开发甘薯特异SNP分子标记,本研究利用简化基因组测序技术对甘薯种质材料进行测序,筛选稳定的SNP位点,将其开发为基于HRM技术的分子标记,并在甘薯种质材料中进行验证。通过对23个甘薯种质材料简化基因组测序数据的分析,共发现835 756个SNP多态性位点,筛选其中的3 650个含有SNP多态性位点的高质量测序片段,成功设计了134对引物;初步验证发现,22对(16.42%)引物没有扩增产物,15对(11.19%)引物扩增产物2个以上,36对(26.87%)引物的扩增产物没有差异。61对(45.52%)引物在8个样本中的扩增特异性好,而且样本之间有差异。最后筛选34对扩增多态性丰富的引物对52份甘薯种质材料进行扫描,发现材料间多态性介于27.27%~90.91%之间,平均多态性达到59.35%。聚类分析表明,参试52份甘薯种质材料之间的差异较小,多数材料与骨干亲本南瑞苕、徐薯18之间关系较近,国外引进甘薯材料和地方品种差异较大。建议在今后甘薯育种中尽量选用地方品种和国外甘薯品种,从而更好地拓宽甘薯遗传背景。本研究初步建立了基于简化基因组技术和HRM技术开发甘薯SNP分子标记的新思路,为今后甘薯SNP分子标记开发提供了参考。同时本研究开发的甘薯分子标记,丰富了甘薯分子标记类型,为甘薯研究者开展快速的分子标记研究提供了支持。  相似文献