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991.
Control rats and rats treated with subcutaneous trypan blue were injected intravenously with denatured albumin-I(125). Lysosome-rich fractions of their livers, when incubated at 22 degrees C in osmotically protected medium (pH 7.4), retained their capacit to digest albumin-I(125). The rate of digestion was lower in suspensions pre-pared from rats treated with trypan blue than in control suspensions, but rates of lysosome breakage were not different. T'hese results and other experimental evidence suggest that trypanblue concentrated within lysosomes can inhibit intralysosomal digestion, probably by inhibition of lysosomal proteinases. 相似文献
992.
Collagen synthesis by cells synchronously replicating DNA 总被引:4,自引:0,他引:4
993.
We purified antifreeze proteins from winter-active snow fleas, Hypogastrura harveyi. These 6.5- and 15.7-kilodalton thermolabile proteins are glycine-rich (45% of the residues), and the short isoform is composed of the tripeptide repeat Gly-X-X. This makes them very different from other antifreeze proteins, including two from insects, suggesting independent adaptation to freezing environments. 相似文献
994.
Schindler DW Mills KH Malley DF Findlay DL Shearer JA Davies IJ Turner MA Linsey GA Cruikshank DR 《Science (New York, N.Y.)》1985,228(4706):1395-1401
Experimental acidification of a small lake from an original pH value of 6.8 to 5.0 over an 8-year period caused a number of dramatic changes in the lake's food web. Changes in phytoplankton species, cessation of fish reproduction, disappearance of the benthic crustaceans, and appearance of filamentous algae in the littoral zone were consistent with deductions from synoptic surveys of lakes in regions of high acid deposition. Contrary to what had been expected from synoptic surveys, acidification of Lake 223 did not cause decreases in primary production, rates of decomposition, or nutrient concentrations. Key organisms in the food web leading to lake trout, including Mysis relicta and Pimephales promelas, were eliminated from the lake at pH values as high as 5.8, an indication that irreversible stresses on aquatic ecosystems occur earlier in the acidification process than was heretofore believed. These changes are caused by hydrogen ion alone, and not by the secondary effect of aluminum toxicity. Since no species of fish reproduced at pH values below 5.4, the lake would become fishless within about a decade on the basis of the natural mortalities of the most long-lived species. 相似文献
995.
The effects of auxin on the inhibition of lateral buds in decapitated bean plants are enhanced if kinetin is applied together with auxin. The uptake of (14)C-indoleacetic acid by the stumps of decapitated plants is increased in the presence of kinetin and leads to extensive transport of (14)C-indoleacetic acid in the stems. The increased bud inhibition resulting when auxin and kinetin are applied together may be due to greater amounts of auxin reaching the buds, but an alternative explanation is that metabolites are directed from the buds to the point of hormone application. 相似文献
996.
Observations made from the Viking I orbiter show very little water vapor in the Mars atmosphere in the southern hemisphere (0 to 3 precipitable micrometers) with a gradual increase across the equator to northern latitudes. Maximum amounts between 20 and 30 micrometers have been observed in the short period covered by the observations to date. The season, northern midsummer, corresponds to the beginning of the water vapor cycle in that hemisphere. A strong repetitive diurnal cycling between the solid and vapor phases is observed at a site to the east of the Tharsis Ridge at 10 degrees north latitude; the vapor lies close to the martian surface and is most probably in saturation equilibrium with a surface haze or fog throughout much of the day. 相似文献
997.
Shoreline erosion along man-made lakes in North Dakota and South Dakota has led to the discovery of stratified preceramic cultural remains in an area where previously few such materials have been found. One important exposure, the Walth Bay site, contains a 7000-year sequence of alluvial and eolian deposition capping an abandoned Missouri River terrace. Three distinct soil profiles are associated with successive Late Paleo-Indian, Plains Archaic, and Plains Village Tradition occupations. 相似文献
998.
Reeves MB Davies AA McSharry BP Wilkinson GW Sinclair JH 《Science (New York, N.Y.)》2007,316(5829):1345-1348
Human cytomegalovirus infection perturbs multiple cellular processes that could promote the release of proapoptotic stimuli. Consequently, it encodes mechanisms to prevent cell death during infection. Using rotenone, a potent inhibitor of the mitochondrial enzyme complex I (reduced nicotinamide adenine dinucleotide-ubiquinone oxido-reductase), we found that human cytomegalovirus infection protected cells from rotenone-induced apoptosis, a protection mediated by a 2.7-kilobase virally encoded RNA (beta2.7). During infection, beta2.7 RNA interacted with complex I and prevented the relocalization of the essential subunit genes associated with retinoid/interferon-induced mortality-19, in response to apoptotic stimuli. This interaction, which is important for stabilizing the mitochondrial membrane potential, resulted in continued adenosine triphosphate production, which is critical for the successful completion of the virus' life cycle. Complex I targeting by a viral RNA represents a refined strategy to modulate the metabolic viability of the infected host cell. 相似文献
999.
Ivens AC Peacock CS Worthey EA Murphy L Aggarwal G Berriman M Sisk E Rajandream MA Adlem E Aert R Anupama A Apostolou Z Attipoe P Bason N Bauser C Beck A Beverley SM Bianchettin G Borzym K Bothe G Bruschi CV Collins M Cadag E Ciarloni L Clayton C Coulson RM Cronin A Cruz AK Davies RM De Gaudenzi J Dobson DE Duesterhoeft A Fazelina G Fosker N Frasch AC Fraser A Fuchs M Gabel C Goble A Goffeau A Harris D Hertz-Fowler C Hilbert H Horn D Huang Y Klages S Knights A Kube M Larke N Litvin L Lord A 《Science (New York, N.Y.)》2005,309(5733):436-442
1000.
T. R. Tarn H. De Jong A. M. Murphy G. C. C. Tai W. J. Arsenault J. H. E. Thorpe R. H. Bagnall H. W. Platt D. A. Young H. T. Davies 《American Journal of Potato Research》1995,72(7):409-415
AC Belmont is adapted as a second early cultivar in Eastern Canada. It has a short dormancy. The white-skinned round oval tubers size and bulk early, but more similar to Superior than to Jemseg. The specific gravity of AC Belmont is lower than Superior. When boiled, the tubers of AC Belmont have a good, somewhat moist texture; when baked the texture of AC Belmont is relatively dry. AC Belmont is highly resistant toVerticillium albo-atrum, moderately resistant to PVY,Fusarium coeruleum, F. sambucinum f.6. andRhizoctonia solani. 相似文献