Phytoplasma identity and disease etiology     

Davis RE  Sinclair WA 《Phytopathology》1998,88(12):1372-1376

ABSTRACT Many plant diseases believed to be caused by phytoplasmas were described before phytoplasma groups were delineated through molecular analyses. It is now possible to assess the relationships between phytoplasma identity or classification and specific plant diseases. Data were consistent with the hypothesis of a common ancestral origin of pathogenicity genes in many phytoplasmas and a limited repertoire of plant responses to certain pathogen signals. Observations also were consistent with the hypotheses that the botanical host ranges of some phytoplasmas reflect specificities in transmission by vectors and vector feeding preferences; phytoplasma-insect vector relationships are keys to understanding evolutionary divergence of phytoplasma lineages; small differences in a highly conserved phytoplasma gene may be regarded as potential indicators of separate gene pools; the reliability of a diagnosis based on symptoms must be learned empirically (i.e., through case study for each syndrome); and some discrete diseases can be ascribed to phytoplasma taxa at the 16S rRNA group level, whereas others are clearly associated with phytoplasma taxa below this level.  相似文献   

Genetic Variation Among Vegetative Compatibility Groups of Fusarium oxysporum f. sp. cubense Analyzed by DNA Fingerprinting     

Bentley S  Pegg KG  Moore NY  Davis RD  Buddenhagen IW 《Phytopathology》1998,88(12):1283-1293

ABSTRACT Genetic variation within a worldwide collection of 208 isolates of Fu-sarium oxysporum f. sp. cubense, representing physiological races 1, 2, 3, and 4 and the 20 reported vegetative compatibility groups (VCGs), was analyzed using modified DNA amplification fingerprinting. Also characterized were 133 isolates that did not belong to any of the reported VCGs of F. oxysporum f. sp. cubense including race 3 isolates from a Heliconia species and isolates from a symptomatic wild banana species growing in the jungle in peninsular Malaysia. The DNA fingerprint patterns were generally VCG specific, irrespective of geographic or host origin. A total of 33 different genotypes were identified within F. oxysporum f. sp. cu-bense; 19 genotypes were distinguished among the isolates that belonged to the 20 reported VCGs, and 14 new genotypes were identified among the isolates that did not belong to any of the existing VCGs. DNA fingerprinting analysis also allowed differentiation of nine clonal lineages within F. oxysporum f. sp. cubense. Five of these lineages each contained numerous closely related VCGs and genotypes, and the remaining four lineages each contained a single genotype. The genetic diversity and geographic distribution of several of these lineages of F. oxysporum f. sp. cubense suggests that they have coevolved with edible bananas and their wild diploid progenitors in Asia. DNA fingerprinting analysis of isolates from the wild pathosystem provides further evidence for the coevolution hypothesis. The genetic isolation and limited geographic distribution of four of the lineages of F. oxysporum f. sp. cubense suggests that the pathogen has also arisen independently, both within and outside of the center of origin of the host.  相似文献   

Report on the analyses of mAb reactive with porcine CD8 for the second international swine CD workshop     

F. A. Zuckermann  M. D. Pescovitz  B. Aasted  J. Dominguez  I. Trebichavsky  B. Novikov  I. Valpotic  J. Nielsen  S. Arn  D. H. Sachs  J. K. Lunney  P. Boyd  J. Walker  R. Lee  W. C. Davis  I. R. Barbosa  A. Saalmü  ller 《Veterinary immunology and immunopathology》1998,60(3-4):291-303

Based on an analysis of their reactivity with porcine peripheral blood lymphocytes (PBL), only three of the 57 mAbs assigned to the T cell/activation marker group were grouped into cluster T9 along with the two wCD8 workshop standard mAbs 76-2-11 (CD8a) and 11/295/33 (CD8b). Their placement was verified through the use of two-color cytofluorometry which established that all three mAbs (STH101, #090; UCP1H12-2, #139; and PG164A, #051) bind exclusively to CD8⁺ cells. Moreover, like the CD8 standard mAbs, these three mAbs reacted with two proteins with a MW of 33 and 35 kDa from lymphocyte lysates and were, thus, given the wCD8 designation. Because the mAb STH101 inhibited the binding of mAb 76-2-11 but not of 11/295/33, it was given the wCD8a designation. The reactivity of the other two new mAbs in the T9 cluster with the various subsets of CD8⁺ lymphocytes were distinct from that of the other members in this cluster including the standards. Although the characteristic porcine CD8 staining pattern consisting of CD8^low and CD8^high cells was obtained with the mAb UCP1H12-2, a wider gap between the fluorescence intensity of the CD8^low and CD8^high lymphocytes was observed. In contrast, the mAb PG164A, not only exclusively reacted with CD4⁻/CD8^high lymphocytes, but it also failed to recognize CD4/CD8 double positive lymphocytes. It was concluded that this mAb is specific for a previously unrecognized CD8 epitope, and was, thus, given the wCD8c designation. A very similar reactivity pattern to that of PG164A was observed for two other mAbs (STH106, #094; and SwNL554.1, #009). Although these two mAbs were not originally positioned in the T cell subgroup because of their reactivity and their ability to inhibit the binding of PG164A, they were given the wCD8c designation. Overall, five new wCD8 mAbs were identified. Although the molecular basis for the differences in PBL recognition by these mAbs is not yet understood, they will be important in defining the role of CD8⁺ lymphocyte subsets in health and disease.  相似文献   

Neonatal Equine Herpesvirus Type 1 Infection on a Thoroughbred Breeding Farm   总被引：2，自引：0，他引：2  

Michael J. Murray  Fabio del  Piero  Stuart C. Jeffrey  Michael S. Davis  Martin O. Furr  Edward J. Dubovi  John A. Mayo 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1998,12(1):36-41

Of 17 foals born on a Thoroughbred breeding farm between March and April 1995, infection with equine herpesvirus type 1 (EHV-1) was associated with neonatal morbidity in 5 foals, 3 of which died or were euthanized. Morbidity and mortality were associated with pulmonary inflammation, and EHV-1 was identified in the lungs of the 3 foals that died. All neonatal EHV-1 infections occurred in foals of mares housed in the same pasture and barn. No other clinical manifestations of EHV-1 infection (eg, abortion, neurologic disease, or respiratory disease) occurred during this outbreak. Three foals were treated with acyclovir (1 died, 2 survived), which may have influenced the clinical outcome in the surviving foals.  相似文献   

CURRENT NOTES ON PHYSIOGRAPHY     

Davis WM 《Science (New York, N.Y.)》1896,3(54):61-62

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CURRENT NOTES ON PHYSIOGRAPHY     

Davis WM 《Science (New York, N.Y.)》1898,7(169):414-415

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CURRENT NOTES ON PHYSIOGRAPHY     

Davis WM 《Science (New York, N.Y.)》1898,7(173):561-563

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Building the Successful Veterinary Practice, Volume 1: Leadership Tools Volume 2: Programs and Procedures     

John Aspley Davis 《Australian veterinary journal》1998,76(10):701-701

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Evaluation by scoring and computerized morphometry of lesions of early Mycoplasma pulmonis infection and ammonia exposure in F344/N rats   总被引：3，自引：0，他引：3  

D M Pinson  T R Schoeb  J R Lindsey  J K Davis 《Veterinary pathology》1986,23(5):550-555

To evaluate lesions of ammonia exposure and Mycoplasma pulmonis infection in gnotobiotic F344/N rats, groups of rats were inoculated with M. pulmonis, exposed to 76 micrograms/liter (100 parts per million) ammonia, or both, or served as controls. Six rats from each group were killed 3, 5, 7, and 9 days after inoculation and their respiratory organs prepared for histologic examination. Lesions were assessed by subjective scoring and computerized morphometry, and results obtained by the two methods were compared to assess agreement of results. Lesions were limited to the nasal passages. Ammonia exposure resulted in hyperplastic and degenerative changes in the anterior nasal epithelium. Lesions of mycoplasmosis were more severe in ammonia-exposed than unexposed rats, but qualitative differences in lesions were not apparent. Results of the two methods agreed in assessment of submucosal inflammatory cell accumulation. Epithelial lesions were difficult to evaluate by both methods in infected, ammonia-exposed rats. In evaluation of lesions of ammonia exposure alone, agreement was adequate. Both methods indicated that exudates were significantly greater in ammonia-exposed infected rats than in non-exposed infected rats. Thus, scoring and morphometry provided similar assessments of differences in lesion severity among the treatment groups.  相似文献   

CURRENT NOTES ON PHYSIOGRAPHY (XV.)     

Davis WM 《Science (New York, N.Y.)》1895,2(35):266-267

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