Canine Clinical Parasitology     

RM Barlow 《Australian veterinary journal》1994,71(6):190-190

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Evaluation of an Immunochromatographic Test to the Diagnosis of Canine Brucellosis Caused by Brucella canis          下载免费PDF全文

LB Keid  JA Diniz  TMFS Oliveira  HL Ferreira  RM Soares 《Reproduction in domestic animals》2015,50(6):939-944

This study evaluated the performance of an immunochromatographic test (ICT) for the diagnosis of canine brucellosis caused by Brucella canis, comparing its results with that of the rapid slide agglutination test with and without the use of 2‐mercaptoethanol and the agar gel immunodiffusion test (AGID). The microbiological culture, PCR and clinical examination were used as reference. According to the results obtained in clinical examination, blood culture, culture of semen and vaginal swab and PCR in blood, semen and vaginal swab, a total of 102 dogs were divided into three groups: B. canis‐infected dogs (Group 1), B. canis‐non‐infected dogs (Group 2) and dogs with suspected brucellosis (Group 3). The diagnostic sensitivity of RSAT, 2ME‐RSAT, AGID and ICT in Group 1 was, respectively, 75%, 37.5%, 27.8% and 89.58%. The diagnostic specificity of RSAT, 2ME‐RSAT, AGID and ICT in Group 2 was, respectively, 91%, 100%, 100%, and 100%. In dogs with suspected brucellosis, 9.67% were RSAT positive, none was positive by 2ME‐RSAT, 3.22% were AGID positive and 6.45% were ICT positive. The main drawback concerning canine brucellosis diagnosis is the lack of a highly sensitive serological assay to be used as a screening test to the rapid identification of infected animals. The ICT showed a high diagnostic specificity and a diagnostic sensitivity value greater than that observed in the RSAT, 2ME‐RSAT and AGID. However, 10.41% of infected dogs had negative results by ICT. These dogs were positive by microbiological culture and/or PCR, indicating active infection and consequently a higher potential of spreading Brucella. Although rapid and simple to perform, the ICT lacked sensitivity to be used as a screening test.  相似文献   

Use of transesophageal echocardiography for visualization of the patent ductus arteriosus during transcatheter coil embolization     

Romain Pariaut DMV  N Sydney Moïse DVM  MS  Marc S Kraus DVM  Anna RM Gelzer Dr.med.vet.  Mark Rishniw BVSc  MS  James A Flanders DVM  Shari Renaud-Farrell VT  Mary Ellen Charter VT 《Journal of Veterinary Cardiology》2004,6(1):32-39

Transesophageal echocardiography (TEE) enhances our ability to see the patent ductus arteriosus in the dog. The improved visualization may potentially improve our ability to perform transcatheter coil embolization in patients that are more likely to have a successful outcome. This report uses still and video images to detail the specifics of coil embolization as performed with the assistance of TEE and compares the images with those of angiography, surgery and postmortem examination.  相似文献   

Qualitative and quantitative evaluation of avian demineralized bone matrix in heterotopic beds     

M. Reza Sanaei DVM  MVSc  Jalila Abu DVM  MS  PhD  Mojgan Nazari DVM  MVSc  Mohd Zuki A.B. DVM  PhD  Zeenathul N. Allaudin DVM  MS  PhD 《Veterinary surgery : VS》2013,42(8):963-970

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Optimization of Protein Recovery During Hydrolysis of Yellowfin Tuna (Thunnus albacares) Visceral Proteins     

Mahmoudreza Ovissipour  Ali Motamedzadegan  Barbara Rasco  Rajab Mohammad Nazari 《Journal Of Aquatic Food Product Technology》2013,22(2):148-159

Protein hydrolysates were prepared from yellowfin tuna viscera using Alcalase®. Response surface methodology (RSM) was applied to study the effect of independent variables, enzyme activity, temperature, and time on the protein recovery (PR) as the response. The coefficient of determination (r² = 98%) was satisfactory, and the lack of fit test showed a non-significant value for the PR model equation, indicating that the regression equation was adequate for predicting the PR under tested combinations of values. Enzyme activity at 79.9 AU/kg protein, temperature at 59.9°C, and the time of 105 min were found to be the optimum conditions to reach the highest PR (85%). Considerable soluble protein was released with increasing degree of hydrolysis (DH). The yellowfin tuna visceral hydrolysates' amino acids profiles showed high essential amino acids content, although lysine, methionine, and phenylalanine were limited. In addition, the results showed that with increasing hydrolysis time, the amino acids increased. Yellowfin tuna visceral hydrolysates were rich in flavor amino acids which can be used as a taste enhancer.  相似文献   

Short‐Term Antiandrogen Flutamide Treatment Causes Structural Alterations in Somatic Cells Associated with Premature Detachment of Spermatids in the Testis of Pubertal and Adult Guinea Pigs     

LR Maschio  RS Cordeiro  SR Taboga  RM Góes 《Reproduction in domestic animals》2010,45(3):516-524

In spite of widespread application of flutamide in the endocrine therapies of young and adult patients, the side effects of this antiandrogen on spermatogenesis and germ‐cell morphology remain unclear. This study evaluates the short‐term androgen blockage effect induced by the administration of flutamide to the testes of pubertal (30‐day old) and adult (65‐ and 135‐day old) guinea pigs, with an emphasis on ultrastructural alterations of main cell types. The testes removed after 10 days of treatment with either a non‐steroidal antiandrogen, flutamide (10 mg/kg of body weight) or a pharmacological vehicle alone were processed for histological, quantitative and ultrastructural analysis. In pubertal animals, flutamide androgenic blockage induces spermatogonial differentiation and accelerates testes maturation, causing degeneration and detachment of primary spermatocytes and round spermatids, which are subsequently found in great quantities in the epididymis caput. In post‐pubertal and adult guinea pigs, in addition to causing germ‐cell degeneration, especially in primary spermatocytes, and leading to the premature detachment of spherical spermatids, the antiandrogen treatment increased the relative volume of Leydig cells. In addition, ultrastructural evaluation indicated that irrespective of age antiandrogen treatment causes an increase in frequency of organelles involved with steroid hormone synthesis in the Leydig cells and a dramatic accumulation of myelin figures in their cytoplasm and, to a larger degree, in Sertoli cells. In conclusion, the transient exposition of the guinea pigs to flutamide, at all postnatal ages causes some degenerative lesions including severe premature detachment of spermatids and accumulation of myelin bodies in Leydig and Sertoli cells, compromising, at least temporarily, the spermatogenesis.  相似文献   

Chirality in synthetic agrochemicals: bioactivity and safety considerations     

N Kurihara  J Miyamoto  GD Paulson  B Zeeh  MW Skidmore  RM Hollingworth  HA Kuiper 《Pest management science》1999,55(2):219-219

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Application of synthetic hormone LHRH-A<Subscript>2</Subscript> on the artificial propagation of Persian sturgeon <Emphasis Type="Italic">Acipenser persicus</Emphasis>     

Rajab Mohammad Nazari  Maryam Modanloo  Mohammad Reza Ghomi  Mahmoud Reza Ovissipor 《Aquaculture International》2010,18(5):837-841

For studying the effect of LHRH-A₂ hormone on the induction of final maturation and ovulation of Persian sturgeon, 71 matured females and 20 matured males were used. Five groups of breeders were injected with sturgeon pituitary gland hormone (50 mg per fish) and LHRH-A₂ in dosages of 3.5, 7, 8 and 10 μg kg⁻¹ for females and 3 and 5 μg kg⁻¹ for males. Results showed that LHRH-A₂ successfully induced final maturation and ovulation in females, and there was no significant difference between five groups of breeders in ovulation proportion, fertilization rate, survival rate of incubation, survival of yolk-sac absorption period and active feeding period of larvae. It can be concluded that the LHRH-A₂ is a proper alternative for pituitary gland hormone in artificial propagation of Persian sturgeon.  相似文献   

Penny ice cap cores, baffin island, canada, and the wisconsinan foxe dome connection: two states of hudson bay ice cover     

DA Fisher  RM Koerner  JC Bourgeois  G Zielinski  C Wake  CU Hammer  HB Clausen  N Gundestrup  S Johnsen  K Goto-Azuma  T Hondoh  E Blake  M Gerasimoff 《Science (New York, N.Y.)》1998,279(5351):692-695

Ice cores from Penny Ice Cap, Baffin Island, Canada, provide continuous Holocene records of oxygen isotopic composition (delta18O, proxy for temperature) and atmospheric impurities. A time scale was established with the use of altered seasonal variations, some volcanic horizons, and the age for the end of the Wisconsin ice age determined from the GRIP and GISP2 ice cores. There is pre-Holocene ice near the bed. The change in delta18O since the last glacial maximum (LGM) is at least 12.5 per mil, compared with an expected value of 7 per mil, suggesting that LGM ice originated at the much higher elevations of the then existing Foxe Dome and Foxe Ridge of the Laurentide Ice Sheet. The LGM delta18O values suggest thick ice frozen to the bed of Hudson Bay.  相似文献   

Strong regularities in world wide web surfing   总被引：5，自引：0，他引：5  

BA Huberman  PLT Pirolli  JE Pitkow  RM Lukose 《Science (New York, N.Y.)》1998,280(5360):95-97

One of the most common modes of accessing information in the World Wide Web is surfing from one document to another along hyperlinks. Several large empirical studies have revealed common patterns of surfing behavior. A model that assumes that users make a sequence of decisions to proceed to another page, continuing as long as the value of the current page exceeds some threshold, yields the probability distribution for the number of pages that a user visits within a given Web site. This model was verified by comparing its predictions with detailed measurements of surfing patterns. The model also explains the observed Zipf-like distributions in page hits observed at Web sites.  相似文献