Amplified fragment length polymorphism for variety identification and genetic diversity assessment in oleander (Nerium oleander L.)   总被引：1，自引：0，他引：1  

Ezio Portis  Cinzia Comino  Anna Lenzi  Piero Lombardi  Romano Tesi  Sergio Lanteri 《Euphytica》2004,136(2):125-137

Oleander is a Mediterranean evergreen shrub found along watercourses, gravelly places and damp slopes. It is grown widely as an ornamental for its abundant and long-lasting flowering as well as its moderate hardiness. Genetic relatedness among 71 accessions, including commercial varieties, different sources of the same varieties, and selections from the wild were investigated using amplified fragment length polymorphism (AFLP). Nine primer combinations yielded a total of 603 bands of which 241 were polymorphic. Genetic similarities among accessions were calculated according to Jaccard's Similarity Index and used to construct a dendrogram based on the unweighted pair group method using arithmetic averages. Our results show that the AFLP technique, which can simultaneously and assay a large number of loci randomly distributed in the genome, is much more informative on the genetic relationship and origin of accessions than the limited number of morphological characters conventionally used for variety discrimination. Up to about 9% molecular genetic differentiation was detected among morphologically indistinguishable provenances of the same variety; this can be partly attributed to scoring error but mainly to somatic variation occurring during vegetative propagation. On the other hand lower genetic distance values were sometimes found among varieties which differ in morphological characters and are thus commercialised with different names. The possibility of considering the amount of genetic variation within a variety as the threshold value for discrimination of initial varieties and essentially derived varieties is discussed. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Application of a low-cost RGB sensor to detect basil (Ocimum basilicum L.) nutritional status at pilot scale level     

Brambilla  Massimo  Romano  Elio  Buccheri  Marina  Cutini  Maurizio  Toscano  Pietro  Cacini  Sonia  Massa  Daniele  Ferri  Serena  Monarca  Danilo  Fedrizzi  Marco  Burchi  Gianluca  Bisaglia  Carlo 《Precision Agriculture》2021,22(3):734-753

Precision Agriculture - In this work, basil plants were fertilized with 0, 2.5 mM and 10 mM nitrogen (with different NO3?/NH4+ ratios), and then monitored using a low-power...  相似文献   

Variations in the Normal Electroencephalogram During a Five-Year Period     

Engel GL  Romano J  Ferris EB 《Science (New York, N.Y.)》1947,105(2736):600-601

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SV40 virus transformation of mouse 3T3 cells does not specifically enhance sugar transport   总被引：3，自引：0，他引：3  

A H Romano  C Colby 《Science (New York, N.Y.)》1973,179(79):1238-1240

The apparent enhancement of 2-deoxy-D-glucose uptake by mouse 3T3 cells accompanying transformation by SV40 virus is not due primarily to an effect on the transport process but to enhanced phosphorylation of the sugar by intracellular kinases. Moreover, the effect is not specifically a function of the presence of the viral genome, but is a reflection of the overall growth rate and physiological state of the cell.  相似文献   

AN IMPROVED METHOD OF DIRECT SMEAR EXAMINATION FOR ACID-FAST BACILLI IN SPUTUMS     

Mishulow L  Melman M  Romano M 《Science (New York, N.Y.)》1934,80(2067):143-144

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Identification of a zinc finger protein that inhibits IL-2 gene expression.   总被引：20，自引：0，他引：20  

T M Williams  D Moolten  J Burlein  J Romano  R Bhaerman  A Godillot  M Mellon  F J Rauscher  J A Kant 《Science (New York, N.Y.)》1991,254(5039):1791-1794

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Bilateral Testicular Mixed Germ Cell-sex Cord-stromal Tumours in a Stallion     

LFC Brito  JB Engiles  RM Turner  LM Getman  A Ebling 《Reproduction in domestic animals》2009,44(5):846-851

An 18-year-old Friesian stallion was examined approximately one week after reportedly presenting scrotal swelling due to torsion of the spermatic cords. Upon presentation no scrotal swelling was noted, the testes were normally oriented, and no abnormalities of the spermatic cords were noted. However, both testes were smaller than expected for a mature stallion and deep palpation revealed that the consistency of the testes was nodular. Ultrasonographic evaluation of the testes revealed diffuse heterogeneous parenchyma with multiple hypoechoic nodular areas. Grossly, the testicular parenchyma was effaced by multiple gray-tan nodules of varying consistency interspersed with gray-white bands of tissue. Microscopic analysis revealed multiple pleomorphic neoplastic foci disseminated throughout both testes. Histological and immunohistochemical features were atypical and consistent with the diagnosis of bilateral testicular mixed germ cell-sex cord stromal tumours. Bilateral testicular tumours and testicular mixed cell tumours are extremely rare in stallions and this is the first report of bilateral testicular mixed germ cell-sex cord-stromal tumours in a stallion. Our findings indicate that certain ultrasonographic characteristics are suggestive of testicular tumour and that immunohistochemistry markers can be used to better characterize testicular neoplasms in stallions.  相似文献   

Distribution of thiobacillus ferrooxidans and leptospirillum ferrooxidans: implications for generation of acid mine drainage   总被引：1，自引：0，他引：1  

MO Schrenk  KJ Edwards  RM Goodman  RJ Hamers  JF Banfield 《Science (New York, N.Y.)》1998,279(5356):1519-1522

Although Thiobacillus ferrooxidans and Leptospirillum ferrooxidans are widely considered to be the microorganisms that control the rate of generation of acid mine drainage, little is known about their natural distribution and abundance. Fluorescence in situ hybridization studies showed that at Iron Mountain, California, T. ferrooxidans occurs in peripheral slime-based communities (at pH over 1.3 and temperature under 30 degreesC) but not in important subsurface acid-forming environments (pH 0.3 to 0.7, temperature 30 degrees to 50 degreesC). Leptospirillum ferrooxidans is abundant in slimes and as a planktonic organism in environments with lower pH. Thiobacillus ferrooxidans affects the precipitation of ferric iron solids but plays a limited role in acid generation, and neither species controls direct catalysis at low pH at this site.  相似文献   

Effect of different dietary microalgae combinations on growth and survival of black‐lip pearl oyster (Pinctada margaritifera) larvae and the feasibility of replacing microalgae with a dietary lipid emulsion          下载免费PDF全文

F. Ehteshami  N. Romano  E. Ramezani Fard  H. Hoseinzadeh Sahafi 《Aquaculture Nutrition》2017,23(4):671-680

A 10‐day experiment was performed to examine different mono, binary and ternary dietary combinations on survival and growth of D‐shaped and umbone black‐lip pearl oyster, Pinctada margaritifera, larvae. The three tropical microalgae species were the flagellate Isochrysis galbana clone T. Iso (CS‐177) and diatoms Chaetoceros calcitrans (CS‐178) and Chaetoceros muelleri (CS‐176) which were fed to D‐shaped and umbone larvae at a density of 7000 and 14 000 cells mL^?1, respectively. A second experiment was performed to investigate the feasibility of replacing T. Iso with a lipid emulsion for both D‐shaped and umbone larvae for 10 and 12 days, respectively. The treatments included only T. Iso, unfed and lipid emulsion to substitute T. Iso at levels of 10% (LIP10), 30% (LIP30) and 100% (LIP100). In the first experiment, results showed that a monospecific diet of T. Iso led to significantly higher (P < 0.05) survival and growth of D‐shaped larvae than all the other treatments. Meanwhile, D‐shaped larval survival was significantly lower when only fed C. calcitrans as well as growth for those fed C. calcitrans or in combination with C. muelleri. However, for umbone larvae, survival and growth were significantly higher when fed a binary combination of T. Iso and C. muelleri or the ternary combination of T. Iso, C. muelleri and C. calcitrans compared with all other treatments. For the second experiment, results showed that with increasing lipid emulsion replacement, survival of both D‐shaped and umbone larvae significantly decreased (P < 0.05); however, the LIP100 treatment was not significantly different (P > 0.05) from the unfed treatment. For D‐shaped larvae, no significant growth difference was detected (P > 0.05) between the T. Iso and LIP10 fed treatments while for umbone larvae, the T. Iso, LIP10 and LIP30 were not significantly different (P > 005). These results indicate that microalgae combinations appear more necessary for later staged P. margaritifera larvae. In addition, the use of a lipid emulsion appeared to provide some nutrition to the larvae, although more research should be conducted to improve the use of such replacements.  相似文献