Effect of Insemination–Ovulation Interval and Addition of Seminal Plasma on Sow Fertility to Insemination of Cryopreserved Sperm     

M Abad  JC Garcia  DJ Sprecher  G Cassar  RM Friendship  MM Buhr  RN Kirkwood 《Reproduction in domestic animals》2007,42(4):418-422

In swine, the use of frozen-thawed (FT) sperm for artificial insemination (AI) is limited because of poor sow fertility, possibly associated with a post-thaw capacitation-like status resulting in fewer fully viable sperm. Sow fertility to AI with FT sperm may improve with deeper deposition of sperm within the female tract, insemination very close to ovulation, or reversal of cryocapacitation by seminal plasma (SP). We performed two experiments to examine these suggestions. In experiment 1, 122 multiparous Yorkshire sows received 600 IU equine chorionic gonadotrophin at weaning and 5 mg pLH 80 h later to control time of ovulation. The predicted time of ovulation (PTO) was 38 h after pLH injection. Thereafter, sows were assigned on the basis of parity to a single AI of FT sperm at 2 h before PTO, or at 12 h before PTO, or FT sperm supplemented with 10% SP at 12 h before PTO. Control sows received fresh semen at 12 h before PTO. All semen doses were adjusted to 3 x 10(9) live cells and deposited into the cervix. Experiment 2 employed 99 multiparous crossbred sows and repeated the treatments of experiment 1 except that all FT inseminations were intrauterine. In both experiments, farrowing rates were lower (p < 0.01) following FT inseminations with no effect of time of insemination or of supplemental SP. In experiment 1, litter size was smaller following FT insemination (p < 0.05), but no effect on litter size was evident in experiment 2. Supplemental SP had no effect on litter size in either experiment. The lack of effect of either SP or timing of FT insemination on sow fertility suggests that the non-lethal sperm cryoinjury affecting fertility involves more than just cryocapacitation.  相似文献   

Toxigenic Vibrio cholerae non-O:1 isolated from a goat in The Netherlands     

I J Visser  E A ter Laak  N W van Dijk  W Wouda 《The Veterinary quarterly》1991,13(2):114-118

A case of enterotoxicosis in a goat at necropsy is described. The animal had died without clinical signs. Toxigenic Vibrio cholerae non-O:1 was isolated from the intestines. This species has not been reported earlier from healthy or diseased farm animals, such as goats, in the Netherlands.  相似文献   

Diagnosis and epidemiology of bovine neosporosis: a review     

Wouda W 《The Veterinary quarterly》2000,22(2):71-74

Neospora caninum is a protozoan parasite that was first described in dogs in 1988. Subsequently the parasite has been associated with abortion in cattle. Evidence is accumulating that neosporosis is a major cause of reproductive failure in cattle all over the world. Recent knowledge of neosporosis is reviewed with emphasis on the diagnosis and epidemiology of the disease in cattle. In addition, attention is paid to strategies for disease control and prevention.  相似文献   

Polymorphs of Alumina Predicted by First Principles: Putting Pressure on the Ruby Pressure Scale     

KT Thomson  RM Wentzcovitch  MST Bukowinski 《Science (New York, N.Y.)》1996,274(5294):1880-1882

Fully optimized quantum mechanical calculations indicate that Al2O3 transforms from the corundum structure to the as yet unobserved Rh2O3 (II) structure at about 78 gigapascals, and it further transforms to Pbnm-perovskite structure at 223 gigapascals. The predicted x-ray spectrum of the Rh2O3 (II) structure is similar to that of the corundum structure, suggesting that the Rh2O3 (II) structure could go undetected in high-pressure x-ray measurements. It is therefore possible that the ruby (Cr3+-doped corundum) fluorescence pressure scale is sensitive to the thermal history of the ruby chips in a given experiment.  相似文献   

An Economics Approach to Hard Computational Problems     

BA Huberman  RM Lukose  T Hogg 《Science (New York, N.Y.)》1997,275(5296):51-54

A general method for combining existing algorithms into new programs that are unequivocally preferable to any of the component algorithms is presented. This method, based on notions of risk in economics, offers a computational portfolio design procedure that can be used for a wide range of problems. Tested by solving a canonical NP-complete problem, the method can be used for problems ranging from the combinatorics of DNA sequencing to the completion of tasks in environments with resource contention, such as the World Wide Web.  相似文献   

A national serological survey to verify Australia's freedom from porcine reproductive and respiratory syndrome   总被引：4，自引：0，他引：4  

MG GARNER  LJ GLEESON  PK HOLYOAKE  RM CANNON  WJ DOUGHTY 《Australian veterinary journal》1997,75(8):596-600

Objective To provide serological data to support Australia's claim of freedom from porcine reproductive and respiratory syndrome.
Design A national serological survey was designed to provide 99% confidence of detecting at least one infected pig herd in Australia, assuming that at least 5% of herds would have been exposed to porcine reproductive and respiratory syndrome virus and that at least 25% of the 'finisher' pigs in these herds would have antibodies to the virus.
Procedure A two-stage testing regime was used. All samples were tested with a commercially available enzyme-linked immunosorbent assay. If assay reactions were found, all samples from the herd were to be tested using the indirect immunofluorescence antibody assay.
Results Of the 875 samples from 163 herds from all States in Australia, there was some evidence of reactivity in only four samples from four herds on the enzyme-linked immunosorbent assay. Further testing using the indirect immunofluorescence antibody assay according to the study protocol demonstrated that the reactions were not due to the presence of specific porcine reproductive and respiratory syndrome virus antibodies in the sera.
Conclusion The results of this study support the view that Australian pigs are free of porcine reproductive and respiratory syndrome virus.  相似文献   

Canine Clinical Parasitology     

RM Barlow 《Australian veterinary journal》1994,71(6):190-190

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Four milk cows dead by electrocution     

Verhoeff J  Hogendoorn MP  Wouda W 《Tijdschrift voor diergeneeskunde》2007,132(24):962-964

Two dairy cows were found dead in their cubicles and a third was found lying dazed in a stall but the animal stood up 5 minutes later. Two days later; two other cows were found dead in the same row of cubicles. Death was due to electrocution caused by an electric wire in a steel pipe connected to front of the cubicles. Post-mortem findings supported this diagnosis.  相似文献   

Evaluation of an Immunochromatographic Test to the Diagnosis of Canine Brucellosis Caused by Brucella canis          下载免费PDF全文

LB Keid  JA Diniz  TMFS Oliveira  HL Ferreira  RM Soares 《Reproduction in domestic animals》2015,50(6):939-944

This study evaluated the performance of an immunochromatographic test (ICT) for the diagnosis of canine brucellosis caused by Brucella canis, comparing its results with that of the rapid slide agglutination test with and without the use of 2‐mercaptoethanol and the agar gel immunodiffusion test (AGID). The microbiological culture, PCR and clinical examination were used as reference. According to the results obtained in clinical examination, blood culture, culture of semen and vaginal swab and PCR in blood, semen and vaginal swab, a total of 102 dogs were divided into three groups: B. canis‐infected dogs (Group 1), B. canis‐non‐infected dogs (Group 2) and dogs with suspected brucellosis (Group 3). The diagnostic sensitivity of RSAT, 2ME‐RSAT, AGID and ICT in Group 1 was, respectively, 75%, 37.5%, 27.8% and 89.58%. The diagnostic specificity of RSAT, 2ME‐RSAT, AGID and ICT in Group 2 was, respectively, 91%, 100%, 100%, and 100%. In dogs with suspected brucellosis, 9.67% were RSAT positive, none was positive by 2ME‐RSAT, 3.22% were AGID positive and 6.45% were ICT positive. The main drawback concerning canine brucellosis diagnosis is the lack of a highly sensitive serological assay to be used as a screening test to the rapid identification of infected animals. The ICT showed a high diagnostic specificity and a diagnostic sensitivity value greater than that observed in the RSAT, 2ME‐RSAT and AGID. However, 10.41% of infected dogs had negative results by ICT. These dogs were positive by microbiological culture and/or PCR, indicating active infection and consequently a higher potential of spreading Brucella. Although rapid and simple to perform, the ICT lacked sensitivity to be used as a screening test.  相似文献   

Use of transesophageal echocardiography for visualization of the patent ductus arteriosus during transcatheter coil embolization     

Romain Pariaut DMV  N Sydney Moïse DVM  MS  Marc S Kraus DVM  Anna RM Gelzer Dr.med.vet.  Mark Rishniw BVSc  MS  James A Flanders DVM  Shari Renaud-Farrell VT  Mary Ellen Charter VT 《Journal of Veterinary Cardiology》2004,6(1):32-39

Transesophageal echocardiography (TEE) enhances our ability to see the patent ductus arteriosus in the dog. The improved visualization may potentially improve our ability to perform transcatheter coil embolization in patients that are more likely to have a successful outcome. This report uses still and video images to detail the specifics of coil embolization as performed with the assistance of TEE and compares the images with those of angiography, surgery and postmortem examination.  相似文献