Progesterone and Caspase-3 Activation in Equine Cyclic Corpora Lutea     

G Ferreira-Dias  L Mateus  AS Costa  S Solá  RM Ramalho  RE Castro  CMP Rodrigues 《Reproduction in domestic animals》2007,42(4):380-386

Soon after ovulation, the newly formed corpus luteum (CL) starts secreting progesterone (P(4)), necessary for implantation. The CL, an ovarian transient endocrine organ, undergoes growth and regression throughout its life span. The objective of this study was to evaluate if caspase-3 mediates cell death in the equine cyclic luteal structures and relate it to luteal endocrine function. Blood and luteal tissue were collected during the breeding season after slaughter from 38 randomly assigned cycling mares. Luteal tissues were classified as corpora haemorrhagica (CH; n = 7); mid luteal phase corpora lutea (Mid-CL; n = 17); late or regressing corpora lutea (Late-CL; n = 9) and corpora albicans (CA; n = 5). Plasma P(4) concentration, determined by radioimmunoassay, showed a significant increase from CH to Mid-CL (p < 0.001), followed by a decrease to Late-CL (p < 0.001) and CA (p < 0.001). Caspase-3 processing and poly (ADP) ribose polymerase (PARP) degradation were assessed by western blotting. Active caspase-3 was twofold increased in Mid-CL, Late-CL and CA as compared with CH (p < 0.05). Immunocytochemistry also showed a significant increase in caspase-3 expression in large luteal cells in all structures when compared with CH (p < 0.05). Consistently, the endogenous caspase-3 substrate, PARP, was markedly degraded from CH to CA (p < 0.05). In fact, the ratio of full-length to degraded PARP showed a significant decrease from CH to Mid-CL, Late-CL and CA (p < 0.05). Finally, the decrease in P(4) from Mid- to Late-CL coincided with no further increases in apoptosis. In conclusion, these results suggest that the effector caspase-3 of apoptosis, might play an important role during luteal tissue involution in the mare, even though its relationship with P(4) remains to be elucidated.  相似文献   

Management Strategies Aiming to Improve Horse Welfare Reduce Embryonic Death Rates in Mares          下载免费PDF全文

E Malschitzky  AM Pimentel  P Garbade  MIM Jobim  RM Gregory  RC Mattos 《Reproduction in domestic animals》2015,50(4):632-636

The objective of this retrospective study was to evaluate the effect of management strategies aiming to improve animal well‐being on pregnancy and embryonic death (ED) rates. Breeding records of a cohort of 1206 Thoroughbred mares brought to a stallion station facility, to be bred with the stallions housed there, were evaluated during ten breeding seasons. Mares were blocked according to management strategies in two groups: Stress and Relax. Strategies used to improve animal well‐being (Relax group) were as follows: stopping the teasing routine, reducing or eliminating stall confinement, reducing the number of mares per group and maintaining herd stability during the breeding season. In barren mares, the pregnancy rate was higher in the Relax group (91.8%) when compared to the observed in Stress group (84.7%). However, no difference in pregnancy rates were observed (Stress = 85.2% vs. Relax = 86.2) in foaling mares. ED rate was higher in barren and foaling mares of the Stress group mares (25.5% and 26.8%, respectively) compared with the Relax group (16.1% and 14.7%, respectively). No significant differences were observed on foal heat pregnancy rate between groups; yet, the embryo loss on foal heat was significant reduced in Relax mares (Relax = 8.7% vs Stress = 24.5%). In conclusion, management strategies aimed to reduce social stress can reduce early pregnancy losses and the average cycles per pregnancy, improving reproductive performance in mares.  相似文献   

Prospective,retrospective or clinical audit: A label that sticks          下载免费PDF全文

C. E. Wylie 《Equine veterinary journal》2015,47(3):257-259

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Synovial sepsis is rare following intrasynovial medication in equine ambulatory practice     

L. C. R. Smith  C. E. Wylie  L. Palmer  P. H. L. Ramzan 《Equine veterinary journal》2019,51(5):595-599

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A systematic literature search to identify performance measure outcomes used in clinical studies of racehorses          下载免费PDF全文

C. E. Wylie  J. R. Newton 《Equine veterinary journal》2018,50(3):304-311

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A longitudinal study of fractures in 1488 Thoroughbred racehorses receiving intrasynovial medication: 2006–2011          下载免费PDF全文

L. C. R. Smith  C. E. Wylie  L. Palmer  P. H. L. Ramzan 《Equine veterinary journal》2018,50(6):774-780

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Expression profiles of immunity and reproductive genes during transition period in Holstein cattle          下载免费PDF全文

AI Ateya  MS Hussein  HM Ghanem  RM Saleh  WB El‐Domany  YY Elseady 《Reproduction in domestic animals》2018,53(2):352-358

The transition period is a critical time for dairy cows as the animal is subjected to the physiological stress accompanying parturition. Immunosuppression and health status were examined during this period in 80 Holstein cows. Blood samples were taken from each cow 3, 2 and 1 week before and after calving, and at calving (0 day). RNA was extracted and subjected to real‐time PCR to determine mRNA levels for the immune‐related genes TLR 2, 4, 6, 7 and β‐defensin 5 in addition to the reproduction‐related genes prolactin and IGF‐I. Results showed significant up‐regulation of pro‐inflammatory‐selected genes, TLR 4, 6 7 and β‐defensin 5 at the third‐week post‐calving; however, earlier periods had lower expression of such genes. In contrast, the immunosuppression biomarker TLR2 gene was up‐regulated at calving and 1 week after parturition and then down‐regulated again at second and third week. Prolactin and IGF‐I genes expression levels were significantly and gradually increased mainly post‐partum. This research highlights that the expression patterns of TLRs, BNBD5, PRL and IGF‐I could be biomarkers to follow up immune and reproductive status of dairy cow at peri‐parturient period to predict the most susceptible risk time for disease incidence and to build up management protocol.  相似文献   

Laser surgery as a treatment for histologically confirmed sarcoids in the horse          下载免费PDF全文

P. C. Compston  T. Turner  C. E. Wylie  R. J. Payne 《Equine veterinary journal》2016,48(4):451-456

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Mineralization of the Equine Palmar/Plantar Annular Ligament Treated by Surgical Resection          下载免费PDF全文

Elaine R. Garvican  Claire E. Wylie  Richard J. Payne  Roger K.W. Smith  Marcus J. Head 《Veterinary surgery : VS》2016,45(5):602-608

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Retracted: Influence of Glutamine Supplementation on Motility and Fertilization Success of Frozen–Thawed Persian Sturgeon (Acipenser persicus) Sperm          下载免费PDF全文

MS Aramli  K Golshahi  RM Nazari  A Golpour  S Aramli 《Reproduction in domestic animals》2016,51(4):474-477

Amino acids have an important biological role for the prevention of cell damage during cryopreservation. The aim of this study was to investigate the effects of glutamine on post‐thaw sperm motility and fertilization success in the Persian sturgeon (Acipenser persicus). Sperm collected from six fish was cryopreserved in extenders containing different glutamine concentrations (2.5, 5 and 10 mm ). Sperm samples diluted at the ratio of 1 : 1 using the extenders were subjected to cryopreservation. After dilution, the sperm suspensions were sucked into 250‐μl straws; the straws were placed on the tray, frozen in nitrogen vapour and plunged into liquid nitrogen. Then, sperm were thawed in a water bath at 40°C for 5 s and used for analysis. Our results revealed that an increase in the concentration of glutamine caused a significant increase in the motility percentage, curvilinear velocity (VCL) and also fertilization success in the Persian sturgeon (p < 0.05). Comparing all concentrations of glutamine, the best concentration for sperm motility and fertilization rate was 10 mm . In addition, higher post‐thaw motility percentage, VCL, and fertilization and hatching rates were obtained with the extender at the concentration of 10 mm (p < 0.05). The findings of this study showed that glutamine was of greater benefit to Persian sturgeon sperm motility during frozen–thawed process.  相似文献