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11.
Defoliation caused annually by Cameraria ohridella has a considerable impact on the general condition of horse chestnut trees. We investigated the consequences of tree endotherapy against this insect. Wood samples, leaves and insect specimens were collected ten years after the treatment (injection) with pesticides. Insect invasion was manifested in all investigated trees by distinct radial growth depression. Treatment resulted in a growth upturn, while non-treated trees maintained decreased growth. The overall foliage infestation level was lower in treated trees, but pupal mass and potential fecundity of insect females were not affected. Our study revealed a long-lasting, positive effect of endotherapy on trees’ appearance and growth.  相似文献   
12.
Broodstock of mature Japanese medaka (Oryzias latipes Temminck and Schlegel, 1846) (mean weight 0.45 ± 0.03 g; age 6 months) were maintained in glass aquaria (8 males and 8 females in each aquarium). The fish were fed commercial feed (group C) or this feed was supplemented with arachidonic acid ARA (2 % of total fatty acids in feed; group A) and one of the COX inhibitors: resveratrol (group AR) or NS-398 (group ANS) with amount 20 mg kg?1 BW day?1 of one of the COX inhibitors. The effect of 2 weeks of feeding experimental diets on reproductive parameters (fecundity, embryo and larval survival, hatching rate, and larvae body weight) was analyzed. Larvae (obtained from experimental dietary treatments) were reared for 2 weeks. The percent of spawning female and fecundity was similar in dietary treatments. COX inhibitors in broodstock diet caused a decrease in embryo survival rate. Eggs of medaka from group ANS and AR revealed the lowest hatchability rate. In contrast, group A revealed a higher hatchability rate at the same time. Larval body weight after hatching was significantly the highest in this group. At the end of the experiment, larvae body weight ranged from 1.62 ± 0.43 mg in group AN to 2.89 ± 0.74 mg in group A. These results show that the COX inhibitors decreased gamete and larval quality regardless of high levels of ARA in broodstock diet.  相似文献   
13.
We monitored leaf phenology and xylogenesis of 12 Pedunculate oaks in northern Poland in 2014. We hypothesized that the individual trees, which differed in size, age and habitat (tree stand or gap), also diverged in terms of the seasonal patterns of leaf phenology and xylogenesis. The samples used for wood formation observations were collected most frequently during the early leaf phenophases (from March to end of June). The transverse sections of the cambial region were cut with a sledge microtome. We counted the number of cambial cells, measured the width of xylem increment and assessed the timing of xylogenesis and earlywood-vessel formation. We found significant differences in leaf phenology and timing of xylogenesis among individual trees. The smallest differences in wood formation among the trees were observed at the beginning of the vegetation season when the first earlywood vessels were detected (9 days). The dates of completion of the first tangential row of earlywood vessels varied by up to 30 days, while for the completion of the entire earlywood dates varied by up to 32 days. The highest productivity of cambial cells (13 cell layers) was observed around the time of bud swelling at mid-April. In the last days of April, the number of cambial cell layers decreased and subsequently increased again when the leaves were nearly fully expanded at the end of May. To summarize, we observed a high seasonal variability in the number of cambial cell layers. Differences in the time of cessation of cambial activity and xylogenesis amounted to 1 month. We conclude that: (1) oak tree-ring widths and earlywood-vessel sizes and numbers may not be sensitive indicators for early spring temperature and spring defoliation; (2) the missing association between leaf phenophases and xylogenesis as well as the phenological variability may be the reasons for the lack of a clear climatic effect on the above-mentioned parameters.  相似文献   
14.
This study was designed to determine the effect of feeding female rats with low-calcium diet containing one of three fructan sources (Jerusalem artichoke, yacon, Beneo Orafti Synergy1), on selected bone parameters. Growing Wistar rats were fed modified AIN-93 G diet enriched in fructan sources (8%), added alone or as a strawberry sorbet ingredient. Two of eight groups were a validation model, where the positive control group was fed with recommended calcium dose in the diet (RCD), and negative one – with low calcium diet (LCD). After 12 weeks, femoral Ca content, bone densitometry, architecture and hardness were examined. The positive effects on femoral Ca content and cortical thickness, area and content in distal part of bone was observed after feeding animals diet enriched in Jerusalem artichoke sorbet. Beneficial action on other bone tomographic parameters (particularly trabecular volumetric bone mineral density) in this part of femur were associated mainly with the consumption of the diet with sorbet containing yacon. Our results showed an important role of diet containing frozen strawberry desserts enriched in fructan sources in the maintenance of healthy bones of growing organism. It may suggest possible synergisms between fructans and bioactive substances of strawberry.  相似文献   
15.
In a natural environment, seminal plasma provides spermatozoa with protection against reactive oxygen species. Storing semen in cooling conditions requires diluting it with various buffer solutions. Therefore, the protective role of seminal plasma is not sufficient enough. Semen obtained from five male specimens was diluted with the Kobayashi buffer solution at a 1:9 ratio. To determine the influence of antioxidants on semen storage, a buffer solution was used, as before, with the addition of 1 % albumin, 1 mM vitamin C, 1.5 mg ml?1 vitamin E, 5 mM sodium citrate, 5 mM glutathione and 5 mM cysteine. After the preparation of such tests, the parameters of spermatozoa motility were measured every 3–5 days, using the CASA system (Image House CRISMAS Company Ltd.). Among all used antioxidants, the best effects were observed after the addition of glutathione to semen. After 17 days of storage, the percentage of motile spermatozoa in the samples preserved with glutathione addition was 57 %, while without antioxidant addition, it was 44 %. Furthermore, the addition of cysteine and albumin also resulted in the lengthening of the life span of perch sperm cells. The presence of the remaining antioxidants (vitamins C and E, and sodium citrate) did not have any positive influence on spermatozoa viability, and in these samples, no motile spermatozoa were observed after 12 days of storage. Our data show that dilution of perch sperm with buffered solution might be a promising method for short-term storage.  相似文献   
16.
Milt of the Leuciscus idus L. was collected from five experimental groups, and selected parameters of its quality were analysed for 36 h (group II), 60 h (group III), 84 h (group IV) and 108 h (group V), respectively, after hormonal stimulation with Ovopel (1 granule kg?1 of body weight). The control (group I) fish were not subjected to hormonal stimulation. The highest milt volume was obtained from the fish in group IV (0.70 ± 0.55 mL), where the largest volume of milt expressed per kilogram was also obtained (3.03 ± 1.94 mL kg?1). Significant differences were also found in milt volumes obtained between group I and groups III (P<0.01) and IV (P<0.05). The highest percentage of motile spermatozoa was found in the milt of group IV (59%); significant differences were found between group I and groups II (P<0.01) and III (P<0.001). The value of osmotic pressure of seminal plasma was the highest in group IV (203.19 ± 37.63 mOsm kg?1), and the lowest in group I (118.31 ± 41.13 mOsm kg?1). Parameters determining milt quality and quantity indicate that the period of 60–84 h after hormonal stimulation with Ovopel is optimal for obtaining milt from ide.  相似文献   
17.
Adhesion of sperm to a glass surface can be manifested by the low percentage of motile spermatozoa and lower speed. This crucial factor could disprove the sperm quality prediction from computer-assisted sperm analysis (CASA) results. In our study, this was particularly observed when solution L was used. However, protein supplementation was effective in the reduction in sperm adhesion. We found that albumin increased the sperm motility at concentrations of 0.25 % in solution L and 0.5 % in solution P. Casein was effective in both solutions at 0.25 % concentration. However, in solution L, the motility was lower than that measured in solutions P and L with the supplementation of BSA at 0.25 and 0.5 %. BSA supplementation was especially beneficial in terms of higher speed values regardless of an activation solution. Casein used with solution P allows us to obtain similar results as in the samples activated with BSA supplementation. Casein supplementation to solution L did not increase CASA results to the level observed in the samples activated with BSA added to the solution L. Our data suggest that the selection of an activation solution is one of the most crucial steps in the experiments requiring CASA data. Depending on the composition, different protein supplements should be used to reduce adhesion of sperm to a glass surface. We recommend the use of BSA at the 0.5–1 % concentration because at this level we obtained the highest CASA parameters in both activation media.  相似文献   
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