In vitro Development of Buffalo Oocytes in Media-containing Fluids from Different Size Class Follicles   总被引：1，自引：0，他引：1  

S Nandi  HM Raghu  BM Ravindranatha  PSP Gupta  PV Sarma 《Reproduction in domestic animals》2004,39(1):33-38

Studies were conducted to investigate the effect of supplementation of fluid from different sized class [small (SFF, < 3 mm), medium (MFF, 3-8 mm) and large (LFF, > 8 mm)] of normal and cystic (CFF) ovarian follicles in oocyte culture media on oocyte maturation rate and embryo development in vitro and to test the efficacy of follicular fluid (FF) from different size classes as a whole oocyte maturation medium. Results suggested that FF were capable of developing buffalo oocytes to embryonic stage in vitro although its efficacy was lower than that of serum. Regardless of high maturation rates after in vitro maturation (IVM) in media containing FF or IVM in whole FF, low blastocyst rates were obtained after in vitro fertilization (IVF) and culture of embryos. Follicular fluid from small follicles had significantly (p < 0.05) higher potential of developing buffalo oocytes to embryonic stage in vitro than that from medium and large follicles. Cystic FF was not capable of supporting development of buffalo oocytes in vitro.  相似文献   

Degradation rates in soil of 1-benzyltriazoies and two triazole fungicides     

Shantagouda G. Patil  Peter H. Nicholls  Keith Chamberlain  Geoffrey G. Briggs  Richard H. Bromilow 《Pest management science》1988,22(4):333-342

The degradation rates in soil of 1-benzyltriazole together with six analogues having substituents in the phenyl ring and two commercial triazole fungicides, PP450 and triadimefon, were determined at 15°C and 20 % soil water content. The order of degradation rates of the benzyltriazoles was H > 4-OCH₃ > 4-F>4-Cl.4-≥tert-C₄H_g3,4-diCl>3-CF₃. Thus, in general, persistence was enhanced by electron-withdrawing substituents and by lipophilic groups that increased sorption by soil. Of the commercial fungicides, PP450 was degraded very slowly (half-life 578 days) while triadimefon was quickly converted (half-life 15 days) to the corresponding alcohol, triadimenol, which in turn was degraded very slowly. The effects of temperature and soil water content on rate of degradation were studied for 1-benzyltriazole and 1-(4-fluorobenzyl)triazole. The rate of degradation of 1-benzyltriazole was more sensitive to soil temperature and water content than was that of 1 -(4-fluorobenzyl)triazole. The influence of these results on the input data required by models which simulate persistence in field soil is discussed.  相似文献   

Remote sensing and machine learning for crop water stress determination in various crops: a critical review     

Virnodkar  Shyamal S.  Pachghare  Vinod K.  Patil  V. C.  Jha  Sunil Kumar 《Precision Agriculture》2020,21(5):1121-1155

Precision Agriculture - The remote sensing (RS) technique is less cost- and labour- intensive than ground-based surveys for diverse applications in agriculture. Machine learning (ML), a branch of...  相似文献   

Differential Expression Pattern Of MADS Box Genes in Floral Whorls of Garcinia indica     

Patil  Reshma V.  Pawar  Kiran D. 《Journal of Crop Science and Biotechnology》2019,22(4):363-369

Journal of Crop Science and Biotechnology - Flower formation is one of the main developmental stages in the life cycle of flowering plants that can be used as a model system to elucidate the...  相似文献   

A definitive method for distinguishing cultivated onion from its weedy mimic,Asphodelus fistulosus,at multiple developmental stages     

B R Ughade  V C Khilare  D M Sangale  G A Korhale  P Ingle  A E Tathe  R Patil  G D Khedkar 《Weed Research》2019,59(1):39-48

The onion, Allium cepa, is an important vegetable crop around the world. Globally, India ranks second in onion production, but yields are often much lower than in many other onion producing countries. This study reports on one major contributing factor to low onion yields in India: the presence of a weedy mimic that is morphologically similar to onion until the onset of flowering. We used morphological and molecular methods to identify this mimic as Asphodelus fistulosus, an exotic weed not previously reported in India. Our study indicated that molecular markers derived from the rbcL and/or matK chloroplast genes in a DNA barcoding approach allow accurate identification of this weed using any tissue and even at early developmental stages. Our results also showed that visual examination of seed lots coupled with DNA barcoding analysis of samples of 5–10 individual seeds can be used to confirm identification. The ability to easily identify contaminating material before and during cultivation can allow farmers to minimise production losses and prevent further spreading of this weed.  相似文献   

Sedative effect of detomidine in infant calves.     

P K Peshin  A P Singh  J Singh  D B Patil  D Sharifi 《Acta veterinaria Hungarica》1991,39(3-4):103-107

Detomidine administered intramuscularly at a dose of 10, 20 or 40 micrograms/kg body mass was evaluated for its sedative effects in 15 unfasted infant calves (age: 15-20 days; body mass: 18-33 kg). The drug produced dose-dependent sedation. At a dose of 10 micrograms/kg detomidine produced effective sedation for 30 to 45 min without any observable analgesia. At doses of 20 or 40 micrograms/kg it caused deep sedation, sternal recumbency, and moderate analgesia of the trunk. Hyperglycaemia was recorded at all dose levels. The changes in respiratory rate, rectal temperature, haemoglobin, packed cell volume, total erythrocyte count and plasma concentration of total protein were not significant.  相似文献   

Genetic typing of bovine viral diarrhoea virus isolates from India     

Mishra N  Pattnaik B  Vilcek S  Patil SS  Jain P  Swamy N  Bhatia S  Pradhan HK 《Veterinary microbiology》2004,104(3-4):207-212

Thirteen BVDV isolates collected in four geographic regions of India between 2000 and 2002 were typed in 5'-UTR. To confirm results of genetic typing, selected viruses were also analysed in the N(pro) region. Phylogenetic analysis revealed that all Indian BVDV isolates belong to BVDV-1b (Osloss-like group). Despite a long distance between the farms from which the viruses were isolated there was no correlation between the origin of viral isolates and their position in a phylogenetic tree. Higher genetic similarity of Indian BVDV isolates was observed most probably due to the uncontrolled movement of cattle as well as the uncontrolled use of semen from bulls for breeding of local and farm cattle in different states of India.  相似文献   

Selected animal and plant protein sources affect nutrient digestibility and fecal characteristics of ileally cannulated dogs.     

G E Bednar  S M Murray  A R Patil  E A Flickinger  N R Merchen  G C Fahey 《Archives of Animal Nutrition》2000,53(2):127-140

The objective of this study was to compare ileal and total tract nutrient digestibilities and fecal characteristics of dogs fed selected animal and plant protein sources incorporated into grain-based diets. Four crude protein sources--soybean meal (SBM), poultry meal (PM), poultry by-product meal (PBPM), and beef and bone meal (BBM)--were fed to four ileal cannulated dogs in a 4 x 4 Latin square design. Intakes of dry matter, organic matter, crude protein, and fat by dogs were similar. Total dietary fiber (TDF) intake was highest for the SBM treatment compared to other treatments. Ileal digestibilities of DM, OM, CP, fat, and TDF were not affected by treatment. Total tract digestibility of DM was lower for the BBM and SBM diets, while OM digestibility was lower for the SBM treatment only. Total tract CP digestibility was similar for BBM, PBPM, and SBM treatments and was higher for the PM treatment. As-is fecal excretion [g/d] was greater when dogs received the SBM treatment. Fecal output on a DM basis was higher for the SBM treatment compared to the other treatments. All diets were well utilized by the dogs as assessed by ileal and total tract digestibility data and fecal characteristics.  相似文献   

Hepatoprotective activity of Calotropis procera flowers against paracetamol-induced hepatic injury in rats   总被引：2，自引：0，他引：2  

Ramachandra Setty S  Quereshi AA  Viswanath Swamy AH  Patil T  Prakash T  Prabhu K  Veeran Gouda A 《Fitoterapia》2007,78(7-8):451-454

Hydro-ethanolic extract (70%) of Calotropis procera flowers was prepared and tested for its hepatoprotective effect against paracetamol-induced hepatitis in rats. Alteration in the levels of biochemical markers of hepatic damage like SGPT, SGOT, ALP, bilirubin, cholesterol, HDL and tissue GSH were tested in both treated and untreated groups. Paracetamol (2 g/kg) has enhanced the SGPT, SGOT, ALP, bilirubin and cholesterol levels and reduced the serum levels of HDL and tissue level of GSH. Treatment with hydro-ethanolic extract of C. procera flowers (200 mg/kg and 400 mg/kg) has brought back the altered levels of biochemical markers to the near normal levels in the dose dependent manner.  相似文献   

Progeny evaluation of Jatropha curcas and Pongamia pinnata with comparison to bioproductivity and biodiesel parameters     

Vinod Kumar Patil  Prithviraj Bhandare  Pramod B. Kulkarni  G.R.Naik 《林业研究》2015,(1):137-142

Progeny studies of Jatropha curcas and Pongamia pinnata were carried with respect to bioproductivity,pod and seed characters which is one of the selection methods in tree improvement programmes. Variations in bioproductivity and biodiesel parameters of both the plants were compared every 6 months for 4 years of investigation and analyzed by analysis of variance and correlation coefficient by Pearson’s method using software Graphpad instat 3.06(for Windows and Mac). P. pinnata has better germination rate(71.4 %), 100 pod weight(PW)(311.59 g) and 100 seed weight(SW)(173.46 g) as compared to J. curcas for germination rate(43.2 %), 100 PW(111.29 g) and 100 SW(67.46 g). P. pinnata has strong correlation for plant height to canopy growth(CG)(0.948), collar diameter(CD)(0.994), number of branches per plant(NBP)(0.995) and to number of leaves per branch(NLB)(0.862) as compared to J.curcas which showed good correlation among plant height to CG(0.976), CD(0.970), NBP(0.988), NLB(0.920) and to number of pods per branch(0.657). However, J. curcas depicted negative correlation for pod breadth to seed length(SL)(-0.447), seed breadth(-0.248) and to seed thickness(ST)(-0.364) and among the 100 PW to SL(-0.199), ST(-0.220) and to 100 SW(-0.704). About 4 kg of P. pinnata seeds were required for each liter of crude oil which yields896 ml of biodiesel on transesterification as compared to5.66 kg of J. curcas seeds for a liter of crude oil, producing about 663 ml of biodiesel. The quality of biodiesel meets the major specification of American Society for Testing and Materials(ASTM) standards for biodiesel. The crude glycerin and seed cake obtained as byproduct during biodiesel production were also measured which can be purified and used in composting, animal feeds, pharmaceuticals and cosmetic industries.  相似文献