The Effects of Sample Size on the Outcome of Ovarian Tissue Cryopreservation     

M Ferreira  A Bos-Mikich  N Frantz  JL Rodrigues  AL Brunetto  G Schwartsmann 《Reproduction in domestic animals》2010,45(1):99-102

Cryopreservation of ovarian tissue is known to affect follicular survival. Several variables may be responsible for this. Little attention has focused on the effect of the size of the fragment to be cryopreserved. This study was conducted to assess the effect of the size of the tissue on follicular histology after freezing with 1,2-propanediol. Histological evaluations were performed of control and cryopreserved tissue. Fragments were cut 10 × 3 × 2 mm³ (2 mm group) or 10 × 3 × 4 mm³ (4 mm group). Percentages of normal follicles in control fragments cut into 2 and 4 mm slices were 56% and 34%, respectively. The relative risks to obtain normal follicles in the 2 mm and the 4 mm fragments after cryopreservation were 0.63 and 0.47, respectively. Freezing reduced follicle survival to a significantly greater extent in the larger tissue fragments. There is an increased risk of damage to primary and primordial follicles, when the tissue slices are cut with all dimensions larger than 2 mm.  相似文献   

Application of Laser‐Assisted Microdissection for Gene Expression Analysis of Mammalian Germ Cells     

R. Kenngott  A. AL‐Banaw  M. Vermehren  J. Wendl  F. Sinowatz 《Anatomia, histologia, embryologia》2010,39(3):219-226

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Environmental conditions and isozyme polymorphism in Chenopodium album L.     

A. AL MOUEMAR  J. GASQUEZ 《Weed Research》1983,23(3):141-149

Electrophoretic variations within four enzyme systems (α-esterase, acid phosphatase, malate dehydrogenase and leucine amino peptidase), were studied for various garden and field populations of Chenopodium album. A correlation was found between the degree of polymorphism for the electrophoretic traits and environmental conditions. The amount of phenotypic polymorphism is very high in gardens, intermediate in an untreated and, low in herbicide-treated fields and nil for a triazine resistant population in a maize (Zea mats L.) monoculture. Furthermore, the tetraploid versus hexaploid ratio is higher within garden populations. Higher variability in garden populations is due, in part, to a high proportion of polymorphic phenotypes, and in part to a low effect of selection pressure in such types of habitat. Although there is a difference between phenotypes of populations from acid soils and those from more basic ones, we cannot be sure that the variation of polymorphism is due only to selection.  相似文献   

从肿头综合征鸡分离出产Vero毒素E.coli   总被引：1，自引：1，他引：0  

杨建德  孙涛 《中国预防兽医学报》1999,21(5):325-327

本研究从哈尔滨郊区某鸡场肿头综合征（ Ｓ Ｈ Ｓ）鸡分离出 ３ 株血清型为 Ｏ７８ 的埃希氏大肠杆菌（ Ｅ．ｃｏｌｉ）,从鞍山 Ｓ Ｈ Ｓ病料分离出 １ 株血清型为 Ｏ８ 的 Ｅ．ｃｏｌｉ。并证实这 ４ 株 Ｅ．ｃｏｌｉ 能够对 Ｖｅｒｏ 细胞产生毒性作用,与产 Ｖｅｒｏ 毒素的 Ｅ．ｃｏｌｉ一致。对哈尔滨市郊 Ｓ Ｈ Ｓ病鸡做病理组织学观察,主要变化为头部皮下组织,头盖骨气室及鼻粘膜下纤维素性化脓性炎症,水肿和大肠杆菌性肉芽肿,心、肝、肾等器官炎症变化并有大量异嗜性粒细胞和细菌团块。  相似文献   

Sudden generalized lung atelectasis during thoracotomy following thoracic lavage in 3 dogs     

Eleanor Drynan BVSc  MACVSc  Gabrielle Musk BSc  BVMS  DECVAA  Anthea Raisis PhD  DVA  MACVSc 《Journal of Veterinary Emergency and Critical Care》2012,22(4):476-482

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Immunohistochemical Expression of Cyclooxygenase‐2 (COX‐2) in Feline Endometrial Adenocarcinoma and in Normal and Hyperplastic Endometria          下载免费PDF全文

AL Saraiva  R Payan‐Carreira  F Gärtner  I Santana  A Rêma  LM Lourenço  MA Pires 《Reproduction in domestic animals》2015,50(2):333-340

Cyclooxygenase‐2 (COX‐2) is overexpressed in several human and animal neoplasms, including the human endometrial carcinoma. It has been suggested as a prognostic marker and a potential therapeutic target. This study aimed to (i) clarify histological aspects of feline endometrial adenocarcinomas (FEA) of the papillary serous type and (ii) characterize COX‐2 immunohistochemical expression in normal, hyperplastic and neoplastic endometrium in this species. Archived paraffin‐embedded tissue samples of 33 FEA, eight cystic endometrial hyperplasias (CEHs) and 21 samples of normal, healthy endometrium in the follicular (FS; n = 10) and luteal (LS; n = 11) stages were evaluated. Histological evaluation of haematoxylin and eosin‐stained sections of the FEA revealed a papillary proliferation of neoplastic cells of serous type, accompanied by clear and multinucleated cells. Other architectural arrangements mainly included solid and tubular growth. Randomly distributed areas of necrosis within the tumours were commonly observed. Invasion of the myometrium, of the serosa and of the vascular and/or lymphatic vessels was not constant features. The mean number of mitoses was higher in FEA compared to non‐neoplastic endometrium. COX‐2 scores were lower in FEA (p = 0.003) and CEH (p = 0.05) when compared to normal epithelium (NE). The loss of the membrane apical reinforcement in epithelial cells was observed in FEA samples, which was accompanied by the dislocation of COX‐2 labelling into the cytoplasm and the perinuclear area; in contrast, in epithelial cells in the healthy and hyperplastic endometria, the immunoreaction showed the characteristic pattern of apical membrane reinforcement, suggestive of the membrane polarization. COX‐2 epithelial scores were higher in the FS than in the LS. No differences were found in stromal COX‐2 expression between normal, CEH and FEA groups, but it was higher in the LS than in the FS. In summary, loss of COX‐2 compartmentalization in neoplastic epithelial cells might be one of the molecular events underlying endometrial carcinogenesis.  相似文献   

Preoperative factors associated with hypotension in young anaesthetised dogs undergoing elective desexing          下载免费PDF全文

RS Costa  AL Raisis  G Hosgood  GC Musk 《Australian veterinary journal》2015,93(4):99-104

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Suspected traumatic rupture of the urinary bladder in an alpaca     

AL ANDREWS  RJ RAWLINSON  AL RAISIS  DR HODGSON 《Australian veterinary journal》1995,72(2):73-75

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Dog attacks on postal delivery officers in Queensland     

AL PODBERSCEK  JK BLACKSHAW 《Australian veterinary journal》1991,68(6):215-216

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Pathological changes in the mammary gland and biochemical changes in milk of the goat following oral dosing with leaf of the Avocado (Persea americana)     

AL CRAIGMILL  AA SEAWRIGHT  T. MATTILA †  AJ FROST 《Australian veterinary journal》1989,66(7):206-211

Two varieties of avocado leaves (Persea americana var Guatemalan and var Mexican) were administered to lactating goats. The Mexican variety was without effect. The Guatemalan variety in doses exceeding 20 g fresh leaf per kg bodyweight, produced damage to the mammary gland with decreased milk production. The lesions were characterised by oedema and reddening, with clots in the large ducts. Microscopically, there was widespread degeneration and necrosis of the secretory epithelium, the necrotic cells sloughing into the lumen. There was no significant cellular inflammatory response. Concentrations of antitrypsin in the milk, indicating changes in vascular permeability, increased rapidly 15 h after a single high dose, coinciding with palpable oedema. Concentrations of NAGase, indicating cell damage, increased after 24 h. Goats given multiple doses followed a similar pattern but the initial response was delayed. The toxic principle, and its mode of action in selectively damaging mammary secretory cells, remains to be determined.  相似文献