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Chetukuri?Anuradha Pooran?M.?GaurEmail author Suresh?Pande Kishore?K.?Gali Muthyl?Ganesh Jagdish?Kumar Rajeev?K.?Varshney 《Euphytica》2011,182(1):1-9
Botrytis grey mould (BGM) caused by Botrytis cinerea Pers. ex. Fr. is the second most important foliar disease of chickpea (Cicer arietinum L.) after ascochyta blight. An intraspecific linkage map of chickpea consisting of 144 markers assigned on 11 linkage groups
was constructed from recombinant inbred lines (RILs) of a cross that involved a moderately resistant kabuli cultivar ICCV
2 and a highly susceptible desi cultivar JG 62. The length of the map obtained was 442.8 cM with an average interval length
of 3.3 cM. Three quantitative trait loci (QTL) which together accounted for 43.6% of the variation for BGM resistance were
identified and mapped on two linkage groups. QTL1 explained about 12.8% of the phenotypic variation for BGM resistance and
was mapped on LG 6A. It was found tightly linked to markers SA14 and TS71rts36r at a LOD score of 3.7. QTL2 and QTL3 accounted
for 9.5 and 48% of the phenotypic variation for BGM resistance, respectively, and were mapped on LG 3. QTL 2 was identified
at LOD 2.7 and flanked by markers TA25 and TA144, positioned at 1 cM away from marker TA25. QTL3 was a strong QTL detected
at LOD 17.7 and was flanked by TA159 at 12 cM distance on one side and TA118 at 4 cM distance on the other side. This is the
first report on mapping of QTL for BGM resistance in chickpea. After proper validation, these QTL will be useful in marker-assisted
pyramiding of BGM resistance in chickpea. 相似文献
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Rajeev Padbhushan 《Journal of plant nutrition》2015,38(8):1143-1157
A greenhouse experiment with green gram on boron (B) deficient calcareous soils was conducted for two years at Ludhiana (Punjab), India to study soil B fractions and response of green gram to B application. Three soils with calcium carbonate (CaCO3) content 0.8 (Soil I), 2.1 (Soil II) and 4.6 (Soil III) percent were collected from different sites of Ludhiana and Bhatinda districts, Punjab, India. The treatments comprised of five levels of soil applied B (0, 0.5, 0.75, 1.0, and 1.5 mg B kg?1). The experiment was laid out in completely randomized design (CRD) factorial design with three replications. Readily soluble B comprised 0.39 to 0.76 percent in Soil I, 0.32 to 0.54 percent in Soil II, and 0.21 to 0.34 percent in Soil III of the total B, taking into account of all the levels of B applied at both stages of crop growth. Readily soluble B increased with increasing application rates of B and decreased from grand growth stage (40 days after sowing) to maturity of the crop. Specifically adsorbed, oxide bound, residual, and total B was higher in Soil III as compared to Soil II or Soil I. At maturity, specifically adsorbed B converted into other fractions to maintain equilibrium in soil solution. Organically bound B was greater than oxide bound B. Among all fractions, the residual fraction accounted for the major fraction of the total B. Soil application of lowest level of B was adequate to cause significant increase in dry matter yield and seed yield of green gram regardless of type of soil. 相似文献
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Bitter-gourd (Momordica charantia L.) cv. ‘Jhallari’ plants were grown in refined sand at graded levels of calcium (Ca) supply ranging from acute deficiency (0.02 mM) to excess (8 mM). Apart from the production of characteristic visible symptoms of the respective stresses, deficient (< 4 mM) or excess (8mM) supply of Ca restricted plant biomass and fruit yield. In leaves, the concentration of chlorophyll, Hill reaction activity and the activities of catalase, peroxidase, acid invertase, ATPase, and α-amylase were reduced, but the activity of acid phosphatase was increased by deficiency of Ca. Excess Ca (8 mM) supply significantly reduced the concentration of chlorophyll, Hill reaction activity and the activity of catalase and increased the specific activities of peroxidase, acid invertase, ATPase, α -amylase, and acid phosphatase. Tissue concentration of Ca in leaves and fruits increased with increase in Ca supply from 0.02 to 8 mM. Tissue concentrations in young leaves representing the thresholds of deficiency or toxicity of Ca are found to be 1.12 and 1.98%, respectively. The critical tissue concentration in these leaves suggestive of severe deficiency of Ca is 0.85%. 相似文献
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Sharanabasappa B. Yeri Kenta Shirasawa Manish K. Pandey M. V. C. Gowda Vanhi Sujay Manda Shriswathi Hajisaheb L. Nadaf Babu N. Motagi S. Lingaraju A. R. S. Bhat Rajeev K. Varshney P. U. Krishnaraj Ramesh S. Bhat 《Plant Breeding》2014,133(1):80-85
Heterogeneous inbred families segregating for rust resistance were identified from the two crosses involving susceptible (TAG 24 and TG 26) and resistant (GPBD 4) varieties of peanut. Rust‐resistant (less than score 5) and rust‐susceptible (more than score 5) plants were identified in each HIF and evaluated under rust epiphytotic conditions. The set of plants belonging to the same HIF, but differing significantly in rust resistance, not in other morphological and productivity traits, was regarded as near‐isogenic lines (NILs). Largely, rust‐resistant NILs had GPBD 4‐type allele, and susceptible NILs carried either TAG 24 or TG 26‐type allele at the three SSR loci (IPAHM103, GM1536 and GM2301) linked to a major genomic region governing rust resistance. Comparison of the remaining genomic regions between the NILs originating from each of the HIFs using transposon markers indicated a considerably high similarity of 86.4% and 83.1% in TAG 24 × GPPBD 4 and TG 26 × GPBD 4, respectively. These NILs are useful for fine mapping and expression analysis of rust resistance. 相似文献
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Michael W. Pennington Shih Chieh Chang Satendra Chauhan Redwan Huq Rajeev B. Tajhya Sandeep Chhabra Raymond S. Norton Christine Beeton 《Marine drugs》2015,13(1):529-542
ShK, from the sea anemone Stichodactyla helianthus, is a 35-residue disulfide-rich peptide that blocks the voltage-gated potassium channel Kv1.3 at ca. 10 pM and the related channel Kv1.1 at ca. 16 pM. We developed an analog of this peptide, ShK-186, which is currently in Phase 1b-2a clinical trials for the treatment of autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. While ShK-186 displays a >100-fold improvement in selectivity for Kv1.3 over Kv1.1 compared with ShK, there is considerable interest in developing peptides with an even greater selectivity ratio. In this report, we describe several variants of ShK that incorporate p-phophono-phenylalanine at the N-terminus coupled with internal substitutions at Gln16 and Met21. In addition, we also explored the combinatorial effects of these internal substitutions with an alanine extension at the C-terminus. Their selectivity was determined by patch-clamp electrophysiology on Kv1.3 and Kv1.1 channels stably expressed in mouse fibroblasts. The peptides with an alanine extension blocked Kv1.3 at low pM concentrations and exhibited up to 2250-fold selectivity for Kv1.3 over Kv1.1. Analogs that incorporates p-phosphono-phenylalanine at the N-terminus blocked Kv1.3 with IC50s in the low pM range and did not affect Kv1.1 at concentrations up to 100 nM, displaying a selectivity enhancement of >10,000-fold for Kv1.3 over Kv1.1. Other potentially important Kv channels such as Kv1.4 and Kv1.6 were only partially blocked at 100 nM concentrations of each of the ShK analogs. 相似文献
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Yadav Ritesh Kumar Gurung Rita Dhakal Rajeev Adhikari Achyut Raj Gautam Subash Ghimire Krishna Hari Sthapit Bhuwon Ratna 《Journal of Crop Science and Biotechnology》2019,22(5):403-414
Journal of Crop Science and Biotechnology - Drawing upon consequentially growing food insecurity in mid-hills attributed to poor adoption levels inter alia inflicted by narrow range of locally... 相似文献
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Haile Desmae Pasupuleti Janila Patrick Okori Manish K. Pandey Babu N. Motagi Emmanuel Monyo Omari Mponda David Okello Dramane Sako Candidus Echeckwu Richard Oteng‐Frimpong Amos Miningou Chris Ojiewo Rajeev K. Varshney 《Plant Breeding》2019,138(4):425-444
Groundnut is an important food and oil crop in the semiarid tropics, contributing to household food consumption and cash income. In Asia and Africa, yields are low attributed to various production constraints. This review paper highlights advances in genetics, genomics and breeding to improve the productivity of groundnut. Genetic studies concerning inheritance, genetic variability and heritability, combining ability and trait correlations have provided a better understanding of the crop's genetics to develop appropriate breeding strategies for target traits. Several improved lines and sources of variability have been identified or developed for various economically important traits through conventional breeding. Significant advances have also been made in groundnut genomics including genome sequencing, marker development and genetic and trait mapping. These advances have led to a better understanding of the groundnut genome, discovery of genes/variants for traits of interest and integration of marker‐assisted breeding for selected traits. The integration of genomic tools into the breeding process accompanied with increased precision of yield trialing and phenotyping will increase the efficiency and enhance the genetic gain for release of improved groundnut varieties. 相似文献