Mass spectrometric detection and formation of D-amino acids in processed plant saps, syrups, and fruit juice concentrates     

Pätzold R  Brückner H 《Journal of agricultural and food chemistry》2005,53(25):9722-9729

Liquid and syrupy dietary saps and juices of plant origin, characterized by the presence of large quantities of saccharides (glucose, fructose, or sucrose) and containing amino acids, were analyzed for the presence of D-amino acids using enantioselective gas chromatography-mass spectrometry. D-amino acids were detected in processed saps and juices of trees (maple, palm, birch), fruits (grape, apple, pear, pomegranate, date), and various other plants (agave, beetroot, sugar cane, carob). D-Ala was detected in all plant products and amounted to approximately 34% D-Ala (relative to L-Ala + D-Ala) in Canadian maple syrups, to approximately 13% in palm saps, and to 48 and 13% D-Ala, respectively, in concentrated grape juices (Spanish Arrope and Turkish Pekmez). Varying amounts and kinds of other D-amino acids were also detected. To test the hypothesis that racemization, that is, partial conversion of L-amino acids into their corresponding D-enantiomers, occurs at reversible stages of the Maillard reaction, the Amadori compound fructose-L-phenylalanine was synthesized. On heating at 200 degrees C for 5 (20) min, release of 10.8% (24.2%) D-Phe was detected. From the data it is concluded that the Amadori compounds formed in the course of the Maillard reaction are pecursors of D-amino acids in foodstuffs.  相似文献   

Determination of the activity of acidic phytate-degrading enzymes in cereal seeds   总被引：2，自引：0，他引：2  

Greiner R  Egli I 《Journal of agricultural and food chemistry》2003,51(4):847-850

Five different methods were compared to elucidate the total activity of the acidic phytate-degrading enzymes present in the seeds of rye, wheat, and barley. Phytate-degrading activity was studied at pH 5.0 by quantifying the liberated phosphate. Rye showed the highest acid phytate-degrading activity among the cereals studied. Using an aqueous extract, only 30-50% of the activity was found (rye, 3443 mU g(-1) of grain; wheat, 1026 mU g(-1) of grain; barley, 1032 mU g(-1) of grain) compared to that found by the direct incubation of the dry-milled cereal grains in a buffered phytate-containing solution (rye, 6752 mU g(-1) of grain; wheat, 2931 mU g(-1) of grain; barley, 2093 mU g(-1) of grain). Extending the extraction time resulted in an increase in extractable phytate-degrading activity by, at maximum, 10-15%. Extraction of phytate-degrading activity is strongly enhanced in the presence of Triton X-100 and the protease inhibitor phenylmethylsulfonyl fluoride (rye, 6536 mU g(-1) of grain; wheat, 2873 mU g(-1) of grain; barley, 2023 mU g(-1) of grain), suggesting at least a partial association with membrane structures and a degradation by proteolytic activity during extraction. In addition, it was shown that determining phytate-degrading activity by quantification of the liberated inorganic phosphate is more robust and precise than determining phytate-degrading activity by quantification of the residual phytate.  相似文献   

Konzeptbodenkarten für den städtischen Raum     

Ralf Grenzius  Prof. Dr. Hans-Peter Blume 《植物养料与土壤学杂志》1993,156(3):209-212

Conception soil maps for urban areas The demand for soil maps increased strongly during the last years. This concerns urban soils, too. Conception soil maps constructed from a lot of basic informations (basic maps and point data) are the alternative to traditional soil mapping, which is expensive in time and money for urban areas. Further informations are to evaluate maps from these conception soil maps. Using graphic information-systems is advantageous for construction and evaluation.  相似文献   

Oxygen profiles and methane turnover in a flooded rice microcosm   总被引：19，自引：0，他引：19  

Peter Frenzel  Franz Rothfuss  Ralf Conrad 《Biology and Fertility of Soils》1992,14(2):84-89

Summary Dissolved O₂ was depleted within the top 3.5-mm surface layer of flooded rice soil microcosms without plants. In planted microcosms, however, O₂ was detectable down to at least 40 mm in depth. O₂ concentrations in the uppermost soil layers of microcosms with rice plants were higher in the light than in the dark, indicating O₂ production by photosynthesis. The CH₄ emission rates were nearly identical for illuminated and for darkened microcosms, demonstrating that the photosynthetically produced O₂ did not increase CH₄ oxidation in the rhizosphere. In contrast, CH₄ emission rates increased when the microcosms were incubated under an N₂ atmosphere, indicating that transport of O₂ from the atmosphere into the rhizosphere was important for CH₄ oxidation. CH₄ emission under air accounted for only 10%–20% of the cumulative CH₄ production determined in cores taken from the microcosms. Apparently, 80%–90% of the CH₄ produced was oxidized in the rhizosphere and thus was not emitted.  相似文献   

Real-time monitoring of 4-vinylguaiacol,guaiacol, and phenol during coffee roasting by resonant laser ionization time-of-flight mass spectrometry     

Dorfner R  Ferge T  Kettrup A  Zimmermann R  Yeretzian C 《Journal of agricultural and food chemistry》2003,51(19):5768-5773

The formation of 4-vinylguaiacol, guaiacol, and phenol during coffee roasting was monitored in real-time, using resonance enhanced multiphoton ionization and time-of-flight mass spectrometry. A model is proposed, based on two connected reaction channels. One channel, termed the "low activation energy" channel, consists of ester hydrolysis of 5-FQA followed by decarboxylation of the ferulic acid to form 4-vinylguaiacol, and finally polymerization at the vinyl group to form partly insoluble polymers (coffee melanoidins). The second "high activation energy" channel opens up once the beans have reached higher temperatures. It leads to formation of guaiacol, via oxidation of 4-vinylguaiacol, and subsequently to phenol and other phenolic VOCs. This work aims at developing strategies to modify the composition of coffee flavor compounds based on the time-temperature history during roasting.  相似文献   

Alexandra Rose Anne Nairn     

Ralf S Mueller 《Australian veterinary journal》1994,71(2):63-63

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Demonstration of two different H2-oxidizing activities in soil using an H2 consumption and a tritium exchange assay     

Veronika Häring  Ralf Conrad 《Biology and Fertility of Soils》1994,17(2):125-128

H₂-oxidizing activities were assayed in slurries of four soils by measuring the consumption of H₂ and the exchange of ³H₂ with H₂O at increasing mixing ratios of H₂ or ³H₂. Both H₂ consumption and ³H₂ exchange were abolished by autoclaving or the addition of formaldehyde. The rates of H₂ consumption and ³H₂ exchange were proportional to the quantity of soil used. Both activities increased with increasing concentrations of H₂ or ³H₂ and displayed biphasic kinetics, demonstrating the existence of two different H₂-oxidizing activities, one with a relatively low K _m and V _max, and a second with a relatively high K _m und V _max. The first type of activity was characteristic of abiontic soil hydrogenases, and the second of aerobic H₂-oxidizing bacteria. In contrast to H₂ consumption, which required the presence of either O₂ or ferricyanide, ³H₂ exchange operated equally well without an external electron acceptor. The ³H₂ exchange assay may thus be particularly useful for enrichment of soil hydrogenases which have not yet been isolated and for which no natural electron acceptor is known.  相似文献   

Detection and species-specific differentiation of pestiviruses using real-time RT-PCR     

Gaede W  Reiting R  Schirrmeier H  Depner KR  Beer M 《Berliner und Münchener tier?rztliche Wochenschrift》2005,118(3-4):113-120

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Cassini dust measurements at Enceladus and implications for the origin of the E ring     

Spahn F  Schmidt J  Albers N  Hörning M  Makuch M  Seiss M  Kempf S  Srama R  Dikarev V  Helfert S  Moragas-Klostermeyer G  Krivov AV  Sremcevic M  Tuzzolino AJ  Economou T  Grün E 《Science (New York, N.Y.)》2006,311(5766):1416-1418

During Cassini's close flyby of Enceladus on 14 July 2005, the High Rate Detector of the Cosmic Dust Analyzer registered micron-sized dust particles enveloping this satellite. The dust impact rate peaked about 1 minute before the closest approach of the spacecraft to the moon. This asymmetric signature is consistent with a locally enhanced dust production in the south polar region of Enceladus. Other Cassini experiments revealed evidence for geophysical activities near Enceladus' south pole: a high surface temperature and a release of water gas. Production or release of dust particles related to these processes may provide the dominant source of Saturn's E ring.  相似文献   

Pemphigus foliaceus in 91 dogs     

Mueller RS  Krebs I  Power HT  Fieseler KV 《Journal of the American Animal Hospital Association》2006,42(3):189-196

A retrospective study of 91 dogs with pemphigus foliaceus was performed. Clinical signs of the disease included crusts (n=79), pustules (n=36), and alopecia (n=33). Lesions were most common on the trunk (n=53), inner pinnae (n=46), face (n=37), and foot pads (n=32). Cytological evaluation revealed acantholytic keratinocytes in 37 of 48 dogs. Results of combination treatment with prednisolone and azathioprine were comparable to results with prednisolone therapy alone. More than half of the dogs achieved remission with appropriate therapy, and another 25% significantly improved.  相似文献