Use of antibodies stained with horseradish with peroxidase for the determination of the presence of antigens     

Z Popsísil  J Franz 《Veterinární medicína》1974,19(8):499-504

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Isolation of ochratoxin A in fodder barley]     

D Veselá  D Vesely  S Jelínek  V Kusák 《Veterinární medicína》1978,23(7):431-436

The authors describe the finding and identification of ochratoxin A in fodder barley of Czechoslovak produce. The fungus Penicillium verrucosum var. verrucosum, which is an ample producer of ochratoxin A, was isolated from a sample of barley. The isolated fungus was cultivated on several substrates at different temperatures and the production of ochratoxin A was studied during cultivation. The authors believe that the ochratoxicoses of farm animals may also occur in Czechoslovakia and that it would be useful to study this mycotoxin in selected feeds.  相似文献   

Etiology and pathogenesis of various respiratory tract infections in cattle diagnosed in Czechoslovakia     

J Mensík 《Veterinární medicína》1971,16(3):131-137

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Effects of various amounts of 3 and 5 per cent Jodonal A solutions on the effectiveness of disinfection in pig-fattening stations]     

K Kubícek  J Hojovec 《Veterinární medicína》1978,23(9):513-520

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Morphological identification of cryptosporidia in the intestines of calves using light and scanning electron microscopy     

J Vítovec 《Veterinární medicína》1982,27(7):419-424

Small and large intestines of ten calves of the age from 7 to 21 days, suffering from cryptosporidiosis were examined. It is recommended to perform the histopathological identification of cryptosporidia by prolongation of the basic staining with haematoxylin to one hour and by additional staining with tartrazine. Out of the special methods the most suitable are as follows: Wolbach's modification of Giems' staining method or staining with toluidine or polychromous blue. Mucopolysaccharides are in the granules and in the capsules of some developmental stages of cryptosporidia. By means of scanning electron microscope (SEM) cryptosporidia are clearly visible. As a rule, they are more or less immersed in the microvilli of enterocytes. In the intestines destructed by inflammation they occur even outside the epithelium, in the cases studied in the necrotic matter obstructing the outlets of the crypts of Lieberkühn in the caecum of the calves.  相似文献   

The effect of administration of non-soluble fungal glucan on the level of delayed skin hypersensitivity and phagocytic activity of leukocytes in the blood of calves]     

S Paulík  V Bajová  J Mojzisová 《Veterinární medicína》1992,37(11):577-585

The level of delayed skin hypersensitivity (DSH) was evaluated in vivo after dinitrofluorobenzene induction (3rd and 7th week of the experimental) while the level of phagocytic activity (FA) of blood leucocytes (in weekly intervals during six weeks of observation) was evaluated in calves (4.5 to 5.5 months of age) after administration of particular fungal glucane (from oyster mushroom) at a dose of 10 mg/kg live weight. In calves which were administered glucane (abbr. G), a significantly stronger cell-mediated immune response was recorded by means of DSH test in the 3rd week of observation, namely in comparison with its starting value (P < 0.01) and also with the value of control animals (P < 0.01; K group)-Tab. I. In the seventh week of the experiment there was not a statistical difference in the average values of DSH any more (Tab. I). But at that time 100 percent of calves showed a skin reaction above 6.5 mm (mean = 7.64 +/- 0.55) evoked by the used sensibilizer in the G group; this reaction was significantly higher (P < 0.01) in comparison with the value of DSH in a majority (80%) of calves of the K group (mean = 6.15 +/- 0.21)-Fig.1. The immunostimulating effect of the used glucane on the FA of blood leucocytes was less significant.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Chlortetracycline residues in the meat of slaughter calves in relation to transmissible microbial resistance to antibiotics     

M Malíková  Z Matyás 《Veterinární medicína》1980,25(10):627-634

The occurrence of chlortetracycline residues in the meat of slaughter calves in 62% herds under study (the additive of the medicated premix AUREOVIT to feed without observing the withdrawal times) did not influence significantly the Gram-negative resistant and R+ microflora isolated from the same material, in comparison with control herds that were administered no antibiotics. Neither was the spectrum of donors and transferring R plasmids influenced significantly nor the incidence of different resistance determinants. The effect of chlortetracycline addition to feed was negative (at P = 0.05) only in the occurrence of R+ microflora isolated from stable environment where the slaughtered calves had been housed.  相似文献   

The therapeutic effectiveness of reduced doses of neomycin, furazolidone and their combinations in the treatment of diarrhea in calves]     

J Habrda  M Malíková  J Bartos 《Veterinární medicína》1977,22(2):91-89

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Molecular evidence of Babesia caballi (Nuttall and Strickland, 1910) parasite transmission from experimentally-infected SCID mice to the ixodid tick, Haemaphysalis longicornis (Neuman, 1901).     

J L Rodríguez Bautista  H Ikadai  M You  B Battsetseg  I Igarashi  H Nagasawa  K Fujisaki 《Veterinary parasitology》2001,102(3):185-191

Molecular evidence that suggests the possible role of the ixodid tick, Haemaphysalis longicornis and its eggs in the transmission of equine Babesia caballi parasites is presented herein. Using polymerase chain reaction (PCR) to assay for DNA in parasites, presumably acquired by ticks that were allowed to feed on splenectomized-SCID mice, experimentally exposed to in vitro-cultivated B. caballi, we have obtained positive bands that corresponded to the expected B. caballi-specific 430bp gene fragment in 50% of female ticks used, and in 75 and 25% of eggs and larval progeny, respectively. Also, parasite DNA was detected in ticks, eggs and larvae as late as the 16th to the 20th day post-host infestation. Present findings support to the potential role of H. longicornis in the transmission of B. caballi parasites. Its capability, however, to successfully transmit the infection to horses under natural conditions in the field needs to be further ascertained. To our knowledge, this is the first documented study incriminating H. longicornis as a most and likely biological vector of equine babesias.  相似文献   

Causes of anaphylactoid reactions in cattle after administration of lipoid preparations]   总被引：1，自引：0，他引：1  

M Toman  J Krejcí  K Pinka  P Mensík 《Veterinární medicína》1992,37(8):417-426

In 1986-1988, adverse anaphylactoid reactions (AR) were observed in animals in Czechoslovakia after the administration of oil adjuvant-containing vaccines or other lipoid drugs. Treated animals showed signs resembling the classic anaphylactic reaction, i.e. restlessness, salivation, pruritus, oedema and cyanosis of udder and vulva, and eyelid oedema, developing within a few minutes. The reactions were not elicited by the antigen alone, but by the oil adjuvant. The aim of our experiments was to identify substances eliciting the reaction in susceptible animals and to investigate possible induction mechanisms. The emulsifier Tween 80 has been demonstrated to be an AR inducing component of vaccines and drugs (Tab. I and III). Weak or moderate reactions were observed in 33% of animals treated with 5% Tween and 66% of those treated with 10% Tween showed strong reactions. On the other hand, no reactions were elicited by treatment with several paraffin oils of different quality (Tab. I) nor with an oil-in-water emulsion containing Montanid as an emulsifier (Tab. II). The role of the vegetative nervous system in the rise of AR has been confirmed. AR were suppressed in animals pretreated with parasympatholytic atropine and enhanced in a part of those pretreated with parasympathomimetic pilocarpine (Tab. III). The percentage of animals affected and the intensity of AR were also lower in animals pretreated with complement inhibitor epsilon-aminocapronic acid (Tab. IV). A major role of complement activation is suggested in the discussion of possible mechanisms of AR induction. It is possible to draw a conclusion on the basis of the results presented here and of the analysis of individual cases that a certain degree of animal susceptibility, depending on the phase of reproductive cycle, metabolism level and neurovegetative balance is necessary besides the administration of an AR inducer (Tween 80 in our case). Hence it seems that the adverse anaphylactoid reactions results from interactions of the two factors, i.e. administration of an AR inducer to susceptible animals.  相似文献