Identification of quantitative trait loci associated with iron deficiency chlorosis resistance in groundnut (Arachis hypogaea)     

Santosh K. Pattanashetti  Manish K. Pandey  Gopalakrishna K. Naidu  Manish K. Vishwakarma  Omprakash Kumar Singh  Yaduru Shasidhar  Ishwar H. Boodi  Basavaraj D. Biradar  Roma Rani Das  Abhishek Rathore  Rajeev K. Varshney 《Plant Breeding》2020,139(4):790-803

Iron deficiency chlorosis is an important abiotic stress affecting groundnut production worldwide in calcareous and alkaline soils with a pH of 7.5–8.5. To identify genomic regions controlling iron deficiency chlorosis resistance in groundnut, the recombinant inbred line population from the cross TAG 24 × ICGV 86031 was evaluated for associated traits like visual chlorosis rating and SPAD chlorophyll meter reading across three crop growth stages for two consecutive years. Thirty-two QTLs were identified for visual chlorosis rating (3.9%–31.8% phenotypic variance explained [PVE]) and SPAD chlorophyll meter reading [3.8%–11% PVE] across three stages over 2 years. This is the first report of identification of QTLs for iron deficiency chlorosis resistance-associated traits in groundnut. Three major QTLs (>10% PVE) were identified at severe stage, while majority of other QTLs were having small effects. Interestingly, two major QTLs for visual chlorosis rating at 60 days (2013) and 90 days (2014) were located at same position on LG AhXIII. The identified QTLs/markers after validation across diverse genetic material could be used in genomics-assisted breeding.  相似文献   

Generation of interspecific hybrids between <Emphasis Type="Italic">Trifolium vesiculosum</Emphasis> and <Emphasis Type="Italic">T. alexandrinum</Emphasis> using embryo rescue     

Ajinder Kaur  Kamal Preet Kaur  Anu Kalia  Upasana Rani  Jagroop Gill Kahlon  Rajesh Sharma  Devendra Malaviya  Rahul Kapoor  Jagdeep Singh Sandhu 《Euphytica》2017,213(11):253

Interspecific hybrids were developed between Trifolium alexandrinum cultivar Wardan × Trifolium vesiculosum and T. alexandrinum cultivar BL1 × T. vesiculosum through embryo rescue, as the crosses failed to set seed under natural conditions. Trifolium vesiculosum was used as a donor/male parent in this study as it is reported to possess tolerance to stem rot and high forage yield. Fertilization in crossed florets of the crosses was manifested from the recovery of swollen ovaries (< 7.80%) and confirmed from the presence of one degenerated ovule in most (> 93.00%) of the swollen ovaries. The hybrid embryos at various developmental stages (heart, torpedo and cotyledonary) were rescued at a frequency of 2.56% from Wardan × T. vesiculosum and 6.12% from BL1 × T. vesiculosum. Differentiation occurred only in the cotyledonary stage embryos, resulting in 17 putative interspecific hybrid plantlets. The assessment of plantlet hybridity through SSR markers (for the alleles inherited from the donor parent), micromorphological leaf traits (leaf texture and stomata) and morphological characters (plant height, leaflet length and width) confirmed production of two interspecific hybrids designated as AV1 and BV3 representing both the crosses. AV1 displayed moderate resistance and BV3 was resistant to stem rot.  相似文献   

Ribotype analysis of Corynebacterium pseudotuberculosis isolates from sheep and goats     

SS SUTHERLAND  RA HART  NB BULLER 《Australian veterinary journal》1993,70(12):454-456

  相似文献   

Comparison of tiletamine-zolazepam-ketamine and tiletamine-zolazepam-ketamine-xylazine anaesthesia in sheep     

HC LIN  SS WALLACE  JW TYLER†  RL ROBBINS  JC THURMON‡  DF WOLFE 《Australian veterinary journal》1994,71(8):239-242

Summary The anaesthetic effects of intravenous tiletamine-zolazepam 6.6 mg/kg-ketamine 6.6 mg/kg (TK) and tiletamine-zolazepam 6.6 mg/kg-ketamine 6.6 mg/kg-xylazine 0.11 mg/kg (TKX) were evaluated in six wethers. Heart rate, respiration rate, arterial blood pressure, and the electrocardiogram were monitored during anaesthesia. Analgesia was tested by electrical stimulation in the left flank. Atropine (0.03 mg/kg) was given intramuscularly before induction, but after recording of baseline heart rate and respiratory rate. The duration of analgesia was 28.7 ± 6.9 min with TK and 82.8 ± 26.6 min with TKX. Heart rate increased significantly within 5 min after TK or TKX administration. Respiratory rate remained unchanged after TK administration, but increased significantly from 5 to 45 min after TKX administration. Arterial blood pressure decreased significantly at 15 min with TK and 30 min with TKX. Sheep remained recumbent for 201 min with TK and 166 min with TKX. All recovered uneventfully. We conclude that either TK or TKX may be used for anaesthetising sheep.  相似文献   

Enhanced disease resistance in the Indian snakehead,Channa punctata against Aeromonas hydrophila,through 5 % feed supplementation with F. benghalensis (aerial root) and L. leucocephala (pod seed)     

Vipin Kumar Verma  Kumari Vandana Rani  Neeta Sehgal  Om Prakash 《Aquaculture International》2015,23(5):1127-1140

  相似文献   

Morphometric and developmental consequences in Spodoptera litura due to feeding on three varieties of sweet potato plants (Ipomoea batata)     

Kurra Sandhyarani  Pathipati Usha Rani 《Phytoparasitica》2013,41(3):317-325

The tobacco caterpillar, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae), is a polyphagous pest defoliating sweet potato, Ipomoea batata (Lam) (Convolvulaceae), throughout Asia. The study of morphometric parameters of S. litura as a result of feeding on three varieties (RNSP1, KIRAN and RNSP3) of host plant revealed that a greater number of egg masses were laid on the KIRAN variety than on the others. I. batata varieties showed statistically significant effects on length, breadth and weight of different larval instars. KIRAN-fed larvae molted to bigger-sized pupae. S. litura larvae reared on KIRAN variety had the highest feeding index as well as growth index. The low concentrations of phenols and carbohydrates coupled with elevated levels of proteins and amino acids were found in KIRAN as compared with RNSP1 and RNSP3. Collectively, the results of this study revealed that KIRAN cultivar was the most suitable food plant for the development of S. litura. Concurrently, it is suggested that this cultivar is therefore least suitable for sweet potato cultivation.  相似文献   

Innovative Technology of COD and BOD Reduction from Coffee Processing Wastewater Using Avocado Seed Carbon (ASC)     

Rani Devi 《Water, air, and soil pollution》2010,207(1-4):299-306

The study estimated the efficiency of Avocado seed carbon (ASC) for chemical oxygen demand (COD) and biochemical oxygen demand (BOD) reduction from coffee processing wastewater. It was performed under batch mode conditions to investigate the optimum operating conditions and efficiency for COD and BOD reduction with ASC compared with commercial activated carbon (CAC). Adsorption isotherm study was also performed and it was found that the values of regression coefficient (r ²), adsorption capacity (k), and adsorption intensity (1/n) for COD and BOD reduction with ASC were comparable to those of CAC. Under optimum operating conditions, the maximum percentage reduction of COD and BOD concentration using ASC was 98.28% and 99.19%, respectively and with CAC was 99.12% and 99.45%, respectively and hence adsorption capacity of ASC is comparable with that of CAC. Thus, this technique may be a good option for treatment of domestic wastewater.  相似文献   

Chemical Oxygen Demand (COD) Reduction in Domestic Wastewater by Fly Ash and Brick Kiln Ash     

Rani Devi  R. P. Dahiya 《Water, air, and soil pollution》2006,174(1-4):33-46

The potential of fly ash, brick kiln ash and commercial activated carbon is determined for the reduction of chemical oxygen demand (COD) from domestic wastewater. Laboratory experiments are conducted for investigating the effect of treatment time, adsorbent dose, pH of the media, initial COD concentration, agitation speed and particle size of adsorbents on the COD reduction from the domestic wastewater. Starting with an initial COD concentration of 1080 mg/l the maximum COD reduction achieved for fly ash was 87.84%, brick kiln ash was 83.22% and commercial activated carbon was 99.35 %. These values were achieved when the wastewater was treated with activated carbon for 180 min, fly ash 250 min and brick kiln ash 300 min and the adsorbent dose was kept respectively at 40 g/l, 60 g/l and 45 g/l for activated carbon, fly ash and brick kiln ash. Agitation speed was kept constant at 600 rpm and the pH was maintained at 2 for activated carbon and fly ash and 5 for brick kiln ash. The maximum percent reduction is for 0.053 mm or smaller size of the particles. Though the adsorption capacity of the ash for reducing the COD is lower than that of the commercial activated carbon, the low material cost can make it an attractive option for the treatment of domestic wastewater.  相似文献   

Evaluation of acute toxicity of phenol and its chloro- and nitro-derivatives to certain teleosts     

S. R. Verma  Saroj Rani  A. K. Tyagi  R. C. Dalela 《Water, air, and soil pollution》1980,14(1):95-102

Acute toxicity bioassays were conducted to evaluate the comparative toxicity of phenol (an aromatic hydrocarbon) pentachlorophenol (chloro-derivative of phenol) and dinitrophenol (nitro-derivative of phenol) for determining the LC50 and acute toxicity range for 24, 48, 72 and 96h, against the test fishes Notopterus notopterus, Colisa fasciatus and Saccobranchus fossilis. Relative susceptibility of fish, safe concentrations, heterogeneity factors and fiducial limits (95 %) were calculated using 96 h LC₅₀ values. Regression equations and slope functions (s) were calculated for each time interval. On the basis of relative susceptibility for phenol, Notopterus notopterus was found to be more susceptible as compared to Saccobranchus fossilis and the susceptibility of Colisa fasciatus lies in between the two. In case of pentachlorophenol and dinitrophenol, susceptibility lies in the increasing order from Colisa fasciatus, Saccobranchus fossilis to Notopterus notopterus.  相似文献   

Hyperactivated Motility in Sperm   总被引：5，自引：0，他引：5  

SS Suarez  H-C Ho 《Reproduction in domestic animals》2003,38(2):119-124

Hyperactivation is a movement pattern seen in sperm at the site and time of fertilization in mammals. It may be critical to the success of fertilization, because it enhances the ability of sperm to detach from the wall of the oviduct, to move around in the labyrinthine lumen of the oviduct, to penetrate mucous substances and, finally, to penetrate the zona pellucida of the oocyte. The movement of hyperactivated sperm appears different under different physical conditions and in different species, but basically it involves an increase in flagellar bend amplitude and, usually, beat asymmetry. Presumably, a signal or signals exist in the oviduct to initiate hyperactivation at the appropriate time; however, none has yet been identified with certainty. While the signal transduction cascade regulating hyperactivation remains to be completely described, it is clear that calcium ions interact with the axoneme of the flagellum to switch on hyperactivation. Although hyperactivation often occurs during the process of capacitation, the two events are regulated by somewhat different pathways.  相似文献