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41.
Luther E. Talbert Jamie D. Sherman Megan L. Hofland Susan P. Lanning Nancy K. Blake Reagan Grabbe Peggy F. Lamb John M. Martin David K. Weaver 《Plant Breeding》2014,133(4):427-432
Several species of wheat stem sawflies (WSS) are pests of wheat, including Cephus cinctus Norton in North America. Larvae feed inside the stems and cut the stem near plant maturity. The primary means of control is resistance due to solid stems, largely controlled by a locus on chromosome 3B (Qss.msub‐3BL). Cultivars that differed for WSS resistance, but with similar stem solidness, were crossed to determine the genetic basis for the differences. The cultivar ‘Scholar’ is susceptible, while ‘Conan’ shows resistance. Scholar and Conan possessed different alleles at Qss.msub‐3BL. Both alleles conferred solidness, yet the Conan allele conferred higher WSS resistance. An allele from Conan on chromosome 4A also decreased infestation and stem cutting. The 3B and 4A alleles from Conan acted in an additive fashion to provide increased WSS resistance without increasing stem solidness. Stem solidness has long been used by breeders as a proxy for WSS resistance because of its simplicity. Our results suggest that other resistance mechanisms may complement solid stems. 相似文献
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Hemolytic anemia was induced in five Domestic Shorthair cats (four adult males and one spayed female obtained from a breeding colony at Colorado State University, CO), and blood samples were analyzed from five other cats (two castrated male Domestic Shorthairs, one castrated male Domestic Longhair, one castrated male Persian, and one spayed female Siamese presented to the Veterinary Teaching Hospital at Colorado State University for miscellaneous problems). Blood samples taken from these cats had percentages of aggregate reticulocytes that ranged from 0% to 14.5% as determined by manual counting and were used to identify the best technique for staining cat reticulocytes for flow cytometric analysis. The best technique was mixing a blood sample (1/2,000 dilution) with 0.2 micrograms thiazole orange in 1 ml of diluent and incubating the mixture in the dark at room temperature for 30 to 60 minutes. The percentage of reticulocytes determined by flow cytometry correlated well (r = 0.88) with manually determined aggregate reticulocyte percentages; no significant differences were observed between the two techniques (P > 0.05). For the conditions used, punctate reticulocytes were not detected by flow cytometry. Samples with very high platelet numbers and very low packed cell volumes may show falsely elevated percentages of reticulocytes as determined by flow cytometry. The reproducibility of the flow cytometric technique was good; the coefficient of variation ranged from 4.8% to 17.9% in two samples with two different times of incubation. Staining of cat aggregate reticulocytes with thiazole orange and use of flow cytometric quantification is a reproducible technique that has a good correlation with the manual reticulocyte counting method. 相似文献
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M D Reagan 《Science (New York, N.Y.)》1969,164(882):926-931