Action of Clathrodin and Analogues on Voltage-Gated Sodium Channels     

Steve Peigneur  Ale? ?ula  Nace Zidar  Fiona Chan-Porter  Robert Kirby  David Madge  Janez Ila?  Danijel Kikelj  Jan Tytgat 《Marine drugs》2014,12(4):2132-2143

Clathrodin is a marine alkaloid and believed to be a modulator of voltage-gated sodium (Na_V ) channels. Since there is an urgent need for small molecule Na_V channel ligands as novel therapeutics, clathrodin could represent an interesting lead compound. Therefore, clathrodin was reinvestigated for its potency and Na_V channel subtype selectivity. Clathrodin and its synthetic analogues were subjected to screening on a broad range of Na_V channel isoforms, both in voltage clamp and patch clamp conditions. Even though clathrodin was not found to exert any activity, some analogues were capable of modulating the Na_V channels, hereby validating the pyrrole-2-aminoimidazole alkaloid structure as a core structure for future small molecule-based Na_V channel modulators.  相似文献   

Short-term competition between crop plants and soil microbes for inorganic N fertilizer     

Erich Inselsbacher  Nina Hinko-Najera Umana  Markus Gorfer  Katrin Ripka  Rebecca Hood-Novotny  Wolfgang Wanek 《Soil biology & biochemistry》2010,42(2):360-372

Agricultural systems that receive high amounts of inorganic nitrogen (N) fertilizer in the form of either ammonium (NH₄⁺), nitrate (NO₃⁻) or a combination thereof are expected to differ in soil N transformation rates and fates of NH₄⁺ and NO₃⁻. Using ¹⁵N tracer techniques this study examines how crop plants and soil microbes vary in their ability to take up and compete for fertilizer N on a short time scale (hours to days). Single plants of barley (Hordeum vulgare L. cv. Morex) were grown on two agricultural soils in microcosms which received either NH₄⁺, NO₃⁻ or NH₄NO₃. Within each fertilizer treatment traces of ¹⁵NH₄⁺ and ¹⁵NO₃⁻ were added separately. During 8 days of fertilization the fate of fertilizer ¹⁵N into plants, microbial biomass and inorganic soil N pools as well as changes in gross N transformation rates were investigated. One week after fertilization 45-80% of initially applied ¹⁵N was recovered in crop plants compared to only 1-10% in soil microbes, proving that plants were the strongest competitors for fertilizer N. In terms of N uptake soil microbes out-competed plants only during the first 4 h of N application independent of soil and fertilizer N form. Within one day microbial N uptake declined substantially, probably due to carbon limitation. In both soils, plants and soil microbes took up more NO₃⁻ than NH₄⁺ independent of initially applied N form. Surprisingly, no inhibitory effect of NH₄⁺ on the uptake and assimilation of nitrate in both, plants and microbes, was observed, probably because fast nitrification rates led to a swift depletion of the ammonium pool. Compared to plant and microbial NH₄⁺ uptake rates, gross nitrification rates were 3-75-fold higher, indicating that nitrifiers were the strongest competitors for NH₄⁺ in both soils. The rapid conversion of NH₄⁺ to NO₃⁻ and preferential use of NO₃⁻ by soil microbes suggest that in agricultural systems with high inorganic N fertilizer inputs the soil microbial community could adapt to high concentrations of NO₃⁻ and shift towards enhanced reliance on NO₃⁻ for their N supply.  相似文献   

Effects of Amylopectin/Amylose Starch Ratio on Growth, Body Composition and Glycemic Response of Sunshine Bass Morone chrysops × M. saxatilis     

Steven  Rawles Rebecca  Lochmann 《Journal of the World Aquaculture Society》2003,34(3):278-288

Manipulation of the ratio of amylopectin (α‐[1,4] and α‐[1,6] linked glucose) to amylose (α‐[1,41 linked glucose) starches in the carbohydrate fraction of the diet has been used to improve carbohydrate and lipid metabolism in mammalian models. A 10‐wk feeding trial was conducted to determine the effect of dietary amylopectin/amylose ratio on growth and composition of growth of advanced sunshine bass (Morone chrysops × M. saxatilis) fingerlings (60 g, initial weight). Fish were fed cold‐pelleted, semipurified, isonitrogenous (35% crude protein), isocaloric (3.6 kcaVg protein), isolipidic (5%) diets containing 25% carbohydrate. The carbohydrate fraction of the diets was composed of either glucose, dextrin, 100% amylopectin/0% amylose, 70% amylopectin/30% amylose, or 30% amylopectin/70% amylose. Diets differing in ratios of amylopectin/amylose were achieved by adjusting the proportion of high‐amylopectin (100% amylopectin) to high‐amylose (70% amylose) corn starch. Diets were fed to fish in quadruplicate 76‐L tanks (seven fish/tank) connected to a brackish water (5‐7%v) recirculating culture system with biofiltration. Weight gain ranged from 195 to 236% of initial weight (60 g) and was significantly greater (P < 0.1) for fish fed diets containing 25% carbohydrate as dextrin or as 70% amylose and significantly lower in fish fed diets in which carbohydrate was composed of 30% amylose, 100% amylopectin, or glucose. Feed efficiency ranged from 0.52 to 0.61 and was higher in fish fed the diet containing the highest concentration of amylose and lower in fish fed the diet containing glucose. Hepatosomatic index was highest (2.71) in fish fed the diet containing glucose and lowest (1.401.45) in fish fed diets containing high‐amylose cornstarch. Intraperitoneal fat ratio was distinctly lower in fish fed diets containing some amylose as compared to those fed diets without amylose. Liver lipid was significantly lower (4.8%) in fish fed the diet containing glucose and almost twice as high (7.3‐8.9%) in fish fed the diets containing any starch. Glycogen content of the liver decreased from approximately 12% in fish fed the diet containing glucose to 5% in fish fed the diets containing amylose. Muscle proximate composition and ratio were unaffected by the dietary treatments. Fasting levels (15 h) of blood glucose in fish reared for 10 wk on the diet containing glucose were significantly elevated (5.5 mmol/L) when compared to fasting levels of those that had been reared on diets containing starch (3.4‐1.1 mmol/L). Fish fed the diet containing glucose exhibited maximum blood concentrations (14.6 mmoVL) 4 h postprandial then rapidly declined to nearly fasting levels within 8 h postprandial. In contrast, maximum plasma glucose concentrations in fish fed diets containing starch were roughly half (6.8‐8.1 mmol/L) those of fish fed the diet containing glucose. Blood glucose in fish fed diets containing dextrin or predominantly amylopectin starch remained elevated longer than that of fish fed diets containing glucose or predominantly amylose starch. Glycemic response appeared to decrease with increasing dietary amylose content. These data suggest that feeding diets in which a greater portion of the starch is amylose may be a useful strategy for improving carbohydrate use in sunshine bass.  相似文献   

Massive Hepatic Necrosis and Nodular Regeneration in Largemouth Bass Fed Diets High in Available Carbohydrate     

Andrew E.  Goodwin  Rebecca T.  Lochmann Darlene M.  Tieman Andrew J.  Mitchell 《Journal of the World Aquaculture Society》2002,33(4):466-477

Largemouth bass Micropterus salmoides are piscivorous fish raised on farms then sold live in Asian fish markets on the east and west coasts of the United States. In the winter of 1998, a major producer of feed‐trained bass suffered a significant increase in fish mortality both during shipping and while the fish were still in ponds. No bacterial, viral, or significant parasitic pathogens were found at necropsy. Livers of affected fish were pale and translucent with 3–10 mm pink nodules on their surface and deeper in the parenchyma. Histological examination of these livers showed that the translucent regions of the liver contained few hepatocytes and were composed of tissue consistent with a chronic inflammatory response. Also present were eosinophils, islands of pancreatic and biliary cells, and granulomas that did not stain positively for mycobacteria. The pink nodules were areas of multifocal regeneration of normal hepatocytes. Sequential studies of bass in ponds revealed that the bass were progressively accumulating glycogen in their hepatocytes to an extent sufficient to explain the massive necrosis of that organ. In order to determine the effect of diets varying in available carbohydrate on fish growth, survival, and liver glycogen content, a 12‐wk feeding trial was conducted in aquaria with juvenile largemouth bass. Nitrogen‐free extract values indicated that an extruded trout diet, a steelhead trout diet, and a diet designed to contain 45% protein and 25% fat, contained 35, 27, and 21% carbohydrate, respectively. Weight gain was lowest in fish fed the extruded trout diet, while liver glycogen was significantly higher in fish fed diets with >27% carbohydrate than in fish fed the diet with 21% carbohydrate. The farmer switched to a diet similar to the 45–25 diet used in our trial. Subsequently, 16‐mo‐old fish examined in October 2000 had no hepatic nodules or necrosis, were significantly larger and less variable in size than previous crops, and the farmer experienced no significant losses in ponds or during shipping.  相似文献   

Nutritional support in critical care patients.   总被引：3，自引：0，他引：3  

Rebecca L Remillard 《Veterinary Clinics of North America: Small Animal Practice》2002,32(5):1145-64, viii

Malnutrition in the form of insufficient nutrient intake to support tissue metabolism undermines appropriate medical or surgical therapeutic management of a case. The major consequences of malnutrition in all patients are decreased immunocompetence, decreased tissue synthesis and repair, and altered intermediary drug metabolism. A practical goal is to begin nutritional support within 24 hours of the injury, illness, or presentation. There are only two methods by which nutrients can be supplied to the body: enteral and parenteral. General guidelines are presented to help establish a foundation.  相似文献   

Identification of trichomonadid protozoa from the bovine preputial cavity by polymerase chain reaction and restriction fragment length polymorphism typing.     

Dawn C Hayes  Rebecca R Anderson  Richard L Walker 《Journal of veterinary diagnostic investigation》2003,15(4):390-394

Accurate identification of the bovine pathogen Tritrichomonas foetus is sometimes complicated by the presence of other trichomonadid protozoa in clinical samples. A highly specific and reproducible approach for differentiating 3 common types of bovine trichomonadid protozoa found in the bovine preputial cavity, T. foetus, Pentatrichomonas hominis, and a Tetratrichomonas species, was developed using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis. Universal trichomonadid protozoa primers, TFR1 and TFR2, were used to amplify the 5.85 rRNA gene and internal transcribed spacer regions (ITSRs), and the products were digested with the restriction enzyme HpyCH4IV. Restriction fragment length polymorphism analysis was performed on 55 trichomonad isolates from bovine preputial washing and scraping samples. The RFLP results correlated 100% with 5.85 rRNA gene and ITSR sequence resultsand PCR results with primers specific for T. foetus. The results of this study demonstrate that PCR and RFLP analysis can be used in lieu of DNA sequencing to identify the specific trichomonadid protozoa isolated from the bovine preputial cavity.  相似文献   

Dietary fats and the skin and coat of dogs     

Kirby NA  Hester SL  Bauer JE 《Journal of the American Veterinary Medical Association》2007,230(11):1641-1644

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Colombian Land­Use Change and the Settlement of the Oriente     

J. M. Kirby 《Asia Pacific viewpoint》1978,19(1):1-25

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Application of biotechnology in breeding lentil for resistance to biotic and abiotic stress     

Frederick J. Muehlbauer  Seungho Cho  Ashutosh Sarker  Kevin E. McPhee  Clarice J. Coyne  P. N. Rajesh  Rebecca Ford 《Euphytica》2006,147(1-2):149-165

Summary Lentil is a self-pollinating diploid (2n = 14 chromosomes) annual cool season legume crop that is produced throughout the world and is highly valued as a high protein food. Several abiotic stresses are important to lentil yields world wide and include drought, heat, salt susceptibility and iron deficiency. The biotic stresses are numerous and include: susceptibility to Ascochyta blight, caused by Ascochyta lentis; Anthracnose, caused by Colletotrichum truncatum; Fusarium wilt, caused by Fusarium oxysporum; Sclerotinia white mold, caused by Sclerotinia sclerotiorum; rust, caused by Uromyces fabae; and numerous aphid transmitted viruses. Lentil is also highly susceptible to several species of Orabanche prevalent in the Mediterranean region, for which there does not appear to be much resistance in the germplasm. Plant breeders and geneticists have addressed these stresses by identifying resistant/tolerant germplasm, determining the genetics involved and the genetic map positions of the resistant genes. To this end progress has been made in mapping the lentil genome and several genetic maps are available that eventually will lead to the development of a consensus map for lentil. Marker density has been limited in the published genetic maps and there is a distinct lack of co-dominant markers that would facilitate comparisons of the available genetic maps and efficient identification of markers closely linked to genes of interest. Molecular breeding of lentil for disease resistance genes using marker assisted selection, particularly for resistance to Ascochyta blight and Anthracnose, is underway in Australia and Canada and promising results have been obtained. Comparative genomics and synteny analyses with closely related legumes promises to further advance the knowledge of the lentil genome and provide lentil breeders with additional genes and selectable markers for use in marker assisted selection. Genomic tools such as macro and micro arrays, reverse genetics and genetic transformation are emerging technologies that may eventually be available for use in lentil crop improvement.  相似文献   

Farmland birds and resource protection in the UK: Cross-cutting solutions for multi-functional farming?     

Richard B. Bradbury  Will B. Kirby 《Biological conservation》2006,129(4):530-542

Farmland bird declines in Europe are well documented. In the UK, agri-environment schemes are key mechanisms for reversing the declines of birds and other farmland biodiversity, but recent reviews suggest that wet habitats might be a gap in provision by these schemes. Important resources provided by wet habitats include: (i) damp soil, for probing species; (ii) permanent water to provide water-dependent invertebrates, as a source of food; (iii) bare or sparsely vegetated ground in the draw-down zone, to improve access to food; (iv) rank emergent vegetation for nesting. However, wet habitats have been lost from farmland as a result of loss of ponds and filling of ditches, as well as the effective removal of water from fields by surface run-off, itself affected by soil compaction, and extensive under-field drainage. The efficient removal of water from fields can cause problems downstream, both through flooding, and diffuse pollution. Regular farmland pollutants include pesticides, nitrogen, phosphorus and sediment, leading to environmental problems such as eutrophication and reduced quality of drinking water. Major new political instruments, such as the Water Framework Directive, will aim to reduce the impact of this diffuse pollution from agriculture. A variety of solutions to diffuse pollution, such as conservation tillage, buffer strips at field edges, and small constructed wetlands, could simultaneously provide some of the resources required by farmland birds. We suggest that future agri-environment schemes, to be truly multifunctional, could focus on bringing these diverse objectives together.  相似文献