Evaluation of a novel proximity ligation assay for the sensitive and rapid detection of foot-and-mouth disease virus   总被引：1，自引：0，他引：1  

Nordengrahn A  Gustafsdottir SM  Ebert K  Reid SM  King DP  Ferris NP  Brocchi E  Grazioli S  Landegren U  Merza M 《Veterinary microbiology》2008,127(3-4):227-236

A novel proximity ligation assay (PLA) using a pan-serotype reactive monoclonal antibody was developed and evaluated for the detection of foot-and-mouth disease virus (FMDV) in clinical samples collected from field cases of disease. The FMDV-specific PLA was found to be 100 times more sensitive for virus detection than the commonly used antigen capture-ELISA (AgELISA). As few as five TCID50 were detected in individual assays, which was comparable with the analytical sensitivity of real-time RT-PCR. Although this assay was capable of detecting diverse isolates from all seven FMDV serotypes, the diagnostic sensitivity of the PLA assay was lower than real-time RT-PCR mainly due to a failure to detect some SAT 1, SAT 2 and SAT 3 FMDV strains. In conclusion, this new PLA format has high analytical sensitivity for the detection of FMDV in clinical samples and may prove valuable as a rapid and simple tool for use in FMD diagnosis.  相似文献   

Pituitary-adrenal function in dogs with acute critical illness     

Martin LG  Groman RP  Fletcher DJ  Behrend EN  Kemppainen RJ  Moser VR  Hickey KC 《Journal of the American Veterinary Medical Association》2008,233(1):87-95

OBJECTIVE: To evaluate pituitary-adrenal function in critically ill dogs with sepsis, severe trauma, and gastric dilatation-volvulus (GDV). DESIGN: Cohort study. ANIMALS: 31 ill dogs admitted to an intensive care unit (ICU) at Washington State University or the University of Pennsylvania; all dogs had acute critical illness for < 48 hours prior to admission. PROCEDURES: Baseline and ACTH-stimulated serum cortisol concentrations and baseline plasma ACTH concentrations were assayed for each dog within 24 hours after admission to the ICU. The change in cortisol concentrations (Delta-cortisol) was calculated for each dog. Morbidity and mortality data were recorded for each patient. RESULTS: Overall, 17 of 31 (55%) acutely critically ill dogs had at least 1 biochemical abnormality suggestive of adrenal gland or pituitary gland insufficiency. Only 1 (3%) dog had an exaggerated response to ACTH stimulation. Dogs with Delta-cortisol < or = 83 nmol/L were 5.7 times as likely to be receiving vasopressors as were dogs with Delta-cortisol > 83 nmol/L. No differences were detected among dogs with sepsis, severe trauma, or GDV with respect to mean baseline and ACTH-stimulated serum cortisol concentrations, Delta-cortisol, and baseline plasma ACTH concentrations. CONCLUSIONS AND CLINICAL RELEVANCE: Biochemical abnormalities of the hypothalamic-pituitary-adrenal axis indicative of adrenal gland or pituitary gland insufficiency were common in critically ill dogs, whereas exaggerated responses to ACTH administration were uncommon. Acutely ill dogs with Delta-cortisol < or = 83 nmol/L may be more likely to require vasopressors as part of the treatment plan.  相似文献   

Prospects for rapid diagnosis of foot-and-mouth disease in the field using reverse transcriptase-PCR     

King DP  Dukes JP  Reid SM  Ebert K  Shaw AE  Mills CE  Boswell L  Ferris NP 《The Veterinary record》2008,162(10):315-316

  相似文献   

Clinical and laboratory investigations of the outbreaks of foot-and-mouth disease in southern England in 2007   总被引：1，自引：0，他引：1  

Ryan E  Gloster J  Reid SM  Li Y  Ferris NP  Waters R  Juleff N  Charleston B  Bankowski B  Gubbins S  Wilesmith JW  King DP  Paton DJ 《The Veterinary record》2008,163(5):139-147

  相似文献   

Development of a vision impairment score for the assessment of functional vision in dogs: Initial evidence of validity,reliability, and responsiveness     

Kathleen L. Graham  Jacky Reid  Cameron J. G. Whittaker  Evelyn J. S. Hall  Kelly Caruso  Christina I. McCowan  Andrew White 《Veterinary ophthalmology》2019,22(6):807-818

  相似文献   

Infection control practices employed within small animal veterinary practices—A systematic review     

Angela Willemsen  Rowland Cobbold  Justine Gibson  Kathryn Wilks  Sheleigh Lawler  Simon Reid 《Zoonoses and public health》2019,66(5):439-457

  相似文献   

Effects of Eimeria acervulina on intestinal pH in conventional and gnotobiotic chickens     

M D Ruff  J K Johnson  D D Dykstra  W M Reid 《Avian diseases》1974,18(1):96-104

  相似文献   

OBSERVATIONS ON THE OYANOGENETIC PROPERTIES OF LINSEED "NUTS".     

M. C. Franklin  M.SC.  A.I.C.  Ph.D. R. L. Reid  M.Sc.Agric. 《Australian veterinary journal》1944,20(6):332-337

  相似文献   

Genetic variability of Trypanosoma evansi isolates detected by inter-simple sequence repeat anchored-PCR and microsatellite     

Njiru ZK  Constantine CC  Gitonga PK  Thompson RC  Reid SA 《Veterinary parasitology》2007,147(1-2):51-60

Studies on genetic variability in Trypanosoma evansi have been limited by a lack of high-resolution techniques. In this study, we have investigated the use of inter-simple sequence repeats (ISSR) and microsatellites in revealing polymorphism among T. evansi isolates. Twelve ISSR primers and five microsatellite loci were used to generate polymorphic bands and alleles, respectively, to investigate the genetic variability among T. evansi isolates from Africa and Asia. Seven of the twelve ISSR primers showed variability between isolates with a total of 71 fragments of which 49(69%) were polymorphic. Microsatellite analysis revealed a total of 60 alleles. On average the ISSR markers revealed a higher genetic diversity (23%) than microsatellites (21.1%). The two techniques showed a strong agreement of r=0.95 for Dice and r=0.91 for Jaccard indices in estimating the genetic distances between isolates. The distance UPGMA tree revealed two major clusters of T. evansi which correlate with the minicircle classification of subtype A and B. The cophenetic correlation coefficient between Dice and Jaccard based matrices were r=0.79 for microsatellites and r=0.73 for ISSR indicating a strong agreement between dendrograms. The results suggest that both ISSR and microsatellites markers are useful in detecting genetic variability within T. evansi.  相似文献   

Infectious salmon anemia in Atlantic salmon.          下载免费PDF全文

J L O''''Halloran  J P L''''Aventure  D B Groman    A M Reid 《The Canadian veterinary journal. La revue veterinaire canadienne》1999,40(5):351-352

  相似文献