Phenotypic characterisation of Australian sheep and cattle isolates of Mannheimia haemolytica, Mannheimia granulomatis and Mannheimia varigena     

PJ BLACKALL  M. BISGAARD  CP STEPHENS 《Australian veterinary journal》2002,80(1):87-91

Objective To perform a comprehensive phenotypic characterisation of 35 isolates of bacteria previously identified as haemolytic Pasteurella‐Actinobacillus and obtained from cattle and sheep. Design The 35 isolates that had been obtained from Australian animals, 30 from cattle and five from sheep, were compared with reference strains of the five recognised species of the genus Mannheimia ‐ M haemolytica, M glucosida, M granulomatis, M ruminalis and M varigena. Results Thirty‐four of the isolates could be confidently assigned to three species of the genus Mannheimia. Twenty‐nine were M haemolytica, with 25 being isolated from cattle and four from sheep. All but three of the bovine M haemolytica were isolated from pneumonic lungs. Of the three remaining bovine M haemolytica isolates, one was obtained in pure culture from a bovine milk sample and the other two as part of a mixed flora associated with a middle ear infection of a calf suffering mucosal disease. Of the four ovine M haemolytica isolates, two were isolated in pure culture from milk and two, also in pure culture, from pneumonic lungs. Three bovine isolates were identified as M granulomatis ‐ one from a tongue abscess, one from a jaw abscess and one from a lung showing suppurative bronchopneumonia. Two bovine isolates were identified as M varigena‐ one coming from an udder and the other from a spleen. The available diagnostic records provided no information on whether these isolates were associated with a disease process. The remaining isolate was obtained from an ovine tongue abscess and could not be assigned to a recognised species within the genus Mannheimia. Conclusion The study represents the first time that M haemolytica, M granulomatis and M varigena have been recognised as being present in cattle and sheep in Australia. Veterinary laboratories that encounter Pasteurella‐Actinobacillus‐like organisms from cattle and sheep should attempt as complete a characterisation as possible to help improve our knowledge of the disease potential of these organsims.  相似文献   

Porcine pleuropneumonia in Australian pigs due to Actinobacillus pleuropneumoniae serovar 5     

CP STEPHENS  JA GIBSON  PJ BLACKALL 《Australian veterinary journal》1990,67(12):462-462

Book reviewed in this article: Guide to the Dissection of Domestic Ruminants . RE Habel Biology and Medicine of Rabbits and Rodents . JE Harkness and JF Wagner  相似文献   

Relationships among bovine male and female reproductive traits     

VEA PERRY  RK MUNRO†  PJ CHENOWETH  DAV BODERO  TB POST† 《Australian veterinary journal》1990,67(1):4-5

No significant relationship (p greater than 0.05) was found between age at puberty in heifers and the age and scrotal circumference at puberty in related bulls. There was a significant effect (p less than 0.01) of genotype and sire on age at puberty of heifers and a significant effect (p less than 0.05) of genotype on weight at puberty in heifers. There was a significant effect of genotype on age (p less than 0.01) and weight (p less than 0.05) at puberty of bulls. A significant difference (p less than 0.05) in age at puberty of bulls was found between the 2 methods of assessing puberty. It is possible that the assessment of puberty of heifers at 2-month intervals may not have been precise enough to detect such a relationship and/or that the variation in genotypes and ages in this study were too great to establish such a relationship.  相似文献   

Characterisation and antimicrobial sensitivity of haemophili isolated from pigs   总被引：2，自引：0，他引：2  

LE. EAVES  PJ. BLACKALL  M. FEGAN 《Australian veterinary journal》1989,66(1):1-4

A total of 70 haemophili from Australia pigs was compared with a range of reference strains of porcine haemophili. Forty-eight of the isolates were identified as Actinobacillus pleuropneumoniae biovar 1 and the remaining 22 isolates as Haemophilus parasuis. Forty one of the A. pleuropneumoniae isolates were used in a study to determine to the minimal inhibitory concentration (MIC) of 12 antimicrobial agents, or combinations of agents. Penicillin, neomycin, trimethoprim, trimethoprim-sulphamethoxazole and tetracycline all showed low MIC values, indicating their potential for the treatment of porcine pleuropneumonia, although 2 isolates showed resistance to tetracycline. A wide range of MIC values was encountered with the sulphonamides.  相似文献   

Bovine ephemeral fever and rhabdoviruses endemic to Australia     

PJ WALKER  DH CYBINSKI 《Australian veterinary journal》1989,66(12):398-400

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Evaluation of an immunofluorescence test on direct smears of conjunctival and urogenital swabs taken from koalas for the detection of Chlamydia psittaci   总被引：1，自引：0，他引：1  

PJ CANFIELD  DN LOVE  G. MEARNS†  E. FARRAM† 《Australian veterinary journal》1991,68(5):165-167

The Chlamydia-Cel Vet IF Test (CCVIT), a commercially available immunofluorescence test for use on direct smears of clinical specimens, was evaluated in a colony of 43 captive koalas. The test is based on a monoclonal antibody directed against the chlamydial common group specific lipopolysaccharide antigen. Swabs were taken from conjuncitva and penis or urogenital sinus and used for direct smear evaluation and cell culture isolation. Compared with isolation of the organism in cell culture, the CCVIT on direct smears of conjunctival swabs presented a sensitivity of 88%, a specificity of 100%, a positive predictive value (PV+) of 100% and a negative predictive value (PV-) of 97%. The CCVIT on direct smears of urogenital swabs presented a sensitivity of 90%, a specificity of 84%, a PV+ of 86% and a PV- of 89%. The overall sensitivity was 89% (95% confidence interval [CI] of 71% to 97%), the specificity 94% (95% CI of 84% to 98%), the PV+ 89% and the PV- 94%. It was concluded that the CCVIT on direct smears was suitable as a diagnostic screening test for the detection of Chlamydia psittaci in koalas.  相似文献   

Smartweeds (Polygonur spp) n photosensitisation of cattle     

RA McKENZIE  PJ DUNSTER  JC BURCHILL 《Australian veterinary journal》1988,65(4):128-128

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Effects of increasing days of exposure to prepartum transition diets on reproduction and health in dairy cows     

PJ DeGaris  IJ Lean  AR Rabiee  C Heuer 《Australian veterinary journal》2010,88(3):84-92

Objective To evaluate the effect of the number of days exposure to a prepartum transition diet on reproduction and health in dairy cows. Design A prospective cohort study. Procedures We enrolled 1008 dairy cows from three herds to examine the effects on reproduction and health of increased days exposure to prepartum transition diets that included ryegrass pasture, ryegrass silage, cereal hay, grain, by-products, oilseed meals, BioChlor®, rumen modifiers, minerals and vitamins. Diets provided 9.9 MJ metabolisable energy per kg dry matter (DM), a metabolisable protein balance of 286 g/day and a dietary cation anion difference of −150 meq/kg DM. Statistical models controlled for herd, calving day, age and gestation period. Results In two of the three herds increased days exposure to prepartum transition diets increased the hazards of submission for breeding, conception and clinical mastitis, and decreased the hazard of cow removal. The odds of pregnancy by 6 and 21 weeks after the mating start date tended to increase with increasing days of exposure to prepartum transition diets. Conclusions Increasing exposure to a prepartum transition diet improved the calving to conception interval, tended to improve the odds of pregnancy and reduced the risk of culling of cattle, but increased the hazard of clinical mastitis.  相似文献   

Detection and characterisation of bovine rotavirus in Ireland from 2006–2008     

PJ Collins  Emily Mulherin  Olivia Cashman  Grainne Lennon  Lynda Gunn  Helen O’Shea  Séamus Fanning 《Irish veterinary journal》2014,67(1):13

Background

Worldwide, Group A bovine rotavirus (RVA boRV) is one of the main causes of neonatal calf diarrhoea. Currently, limited epidemiological and sequence data exists on the RVA disease in bovines in Southern Ireland only. The aim of the study was to generate epidemiological and sequence data of RVA boRV distributed over a wide geographical area in Ireland.

Findings

272 stool samples were obtained from symptomatic calves and analysed to identify the prevalent G and P genotypes. Viral type combinations including G6P[5], G6P[11] and G10P[11] genotype were the most frequently identified. The G6P[5] combination was predominant throughtout the study, accounting for 70% (n = 191). Sequence analysis of the VP7 gene revealed that Irish G6 strains fell within Lineage IV, similiar to previous reports in Ireland.

Conclusion

The detection of unusual G and P combinations may have an impact on rotavirus control programmes and current vaccines may need to incorporate new strains, as the current vaccine available may not offer protection against all of these circulating types.  相似文献   

Comparison of In Vitro Developmental Competence of Cloned Caprine Embryos Using Donor Karyoplasts from Adult Bone Marrow Mesenchymal Stem Cells vs Ear Fibroblast Cells     

PJ Kwong  HY Nam  WE Wan Khadijah  T Kamarul  RB Abdullah 《Reproduction in domestic animals》2014,49(2):249-253

The aim of this study was to produce cloned caprine embryos using either caprine bone marrow‐derived mesenchymal stem cells (MSCs) or ear fibroblast cells (EFCs) as donor karyoplasts. Caprine MSCs were isolated from male Boer goats of an average age of 1.5 years. To determine the pluripotency of MSCs, the cells were induced to differentiate into osteocytes, chondrocytes and adipocytes. Subsequently, MSCs were characterized through cell surface antigen profiles using specific markers, prior to their use as donor karyoplasts for nuclear transfer. No significant difference (p > 0.05) in fusion rates was observed between MSCs (87.7%) and EFCs (91.3%) used as donor karyoplasts. The cleavage rate of cloned embryos derived with MSCs (87.0%) was similar (p > 0.05) to those cloned using EFCs (84.4%). However, the in vitro development of MSCs‐derived cloned embryos (25.3%) to the blastocyst stage was significantly higher (p < 0.05) than those derived with EFCs (20.6%). In conclusion, MSCs could be reprogrammed by caprine oocytes, and production of cloned caprine embryos with MSCs improved their in vitro developmental competence, but not in their fusion and cleavage rate as compared to cloning using somatic cells such as EFCs.  相似文献