Treatment of canine otitis externa using video otoscopy     

Usui R  Usui R  Fukuda M  Fukui E  Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(9):1249-1252

Otitis externa in 27 toy poodles and 40 miniature dachshunds was treated using a video otoscope. A distinct concavity (external tympanic concavity) was observed at the junction between the ventral part of the external surface of the tympanum and the ear canal to which a considerable amount of hair and debris had adhered. All hair and debris adhering to the external tympanic concavity were removed, and systemic antibiotic and antifungal agents were administered, after which all of the dogs recovered. The pattern of hair growth observed in the external tympanic concavity could be characterized according to the breed of dog. All of the toy poodles presented with curly hairs, while the miniature dachshunds had upright or flat-lying hairs.  相似文献   

Histopathologic changes associated with Meibomian Gland dropout in a dog     

Yasunari Kitamura  Reiko Arita  Yukihiro Miwa  Hiroko Iwashita  Akihiko Saito 《Veterinary ophthalmology》2020,23(3):575-578

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Physiological and Mineralogical Properties of Arsenic-Induced Chlorosis in Barley Seedlings Grown Hydroponically     

Molla Rahman Shaibur  Nobuyuki Kitajima  Reiko Sugawara  Toshihito Kondo  S. M. Imamul Huq  Shigenao Kawai 《Journal of plant nutrition》2013,36(2):333-353

The experiment was carried out to investigate the effects of arsenic (As) on the physiological and mineralogical properties of barley (Hordeum vulgare L. cv. ‘Minorimugi’). The plants were grown in nutrient solution treated with 0, 6.7, 33.5, and 67 μ M As (0, 0.5, 2.5, and 5 ppm As, respectively) in the phytotron. Dry matter yield of shoots and roots decreased significantly with the As treatments, indicating that barley plants are As-sensitive and As-toxicity depends on the As concentration in the rooting medium. Necrosis in older leaves and chlorosis symptoms (whitish color) in the fully developed young leaves were observed at the 33.5 and 67 μ M As treatments. Arsenic concentration, accumulation, and translocation increased with the increase of As concentration in the rooting medium. Arsenic was mostly concentrated in roots and a little amount was moved to shoots, indicating that As was not easily translocated to shoots of barley seedlings. Concentrations and accumulations of phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), manganese (Mn), zinc (Zn), and copper (Cu) decreased significantly in shoots for 33.5 and 67 μ M As treatments as compared to the 0 μ M As treatment. Concentrations of P, K, Ca, Mg, Mn, and Cu decreased in roots, but Zn concentration increased in roots at 67 μ M As treatment. Accumulations of P, K, Ca, Mg, Mn, Zn, and Cu in roots also decreased significantly at 67 μ M As treatment. Accumulation of P and the cations showed negative relationship with As. Concentration of Fe decreased in shoots at 33.5 and 67 μ M As treatments where chlorosis was induced in the young leaf but increased in roots at 33.5 and 67 μ M As treatments. It was suggested that As might induce iron (Fe)-chlorosis in the plants. Among the micronutrients, Fe translocation was more affected than others by As. Phytosiderophore (PS) accumulation in roots, which is a symptom of Fe-deficiency in grasses, did not change significantly between 0 and 33.5 μ M As treatments; indicating that As-induced chlorosis did not enhance PS accumulation in roots and decreased due to As-toxicity at 67 μ M As treatment.  相似文献   

Effect of boiled barley-rice-feeding in hypercholesterolemic and normolipemic subjects     

Sachie Ikegami  Masako Tomita  Setsuko Honda  Momoko Yamaguchi  Reiko Mizukawa  Yoko Suzuki  Keiko Ishii  Saeko Ohsawa  Nanako Kiyooka  Mitsuru Higuchi  Shuhei Kobayashi 《Plant foods for human nutrition (Dordrecht, Netherlands)》1996,49(4):317-328

Barley contains approximately 10% dietary fiber and is easily cooked with rice, the dominant cereal in Japan, to increase the intake of dietary fiber. This research involved three experiments to examine the influence of barley on blood lipids in human subjects. All subjects received a boiled barley-rice (50/50 w/w mix) supplement two times per day in place of rice for 2 or 4 weeks. In the normolipemic subjects, serum lipids were unaffected by the ingestion of barley for 4 weeks. In twenty hypercholesterolemic men aged 41 ± 5 years, the ingestion of barley was associated with a significant fall in serum total cholesterol, LDL-cholesterol, phospholipids and LDL and VLDL-lipoproteins. In seven mildly hypercholesterolemic women aged 56±7 years, a significant improvement of serum lipid profiles was observed. The present study suggests the possibility that the ingestion of barley-rice could lower serum lipids in hypercholesterolemic subjects.  相似文献   

Pyogenic meningitis and trigeminal neuritis secondary to periodontogenic paranasal sinusitis in a red deer (Cervus elaphus)     

Kenji KUTARA  Sho KADEKARU  Reiko SUGISAWA  Yumi UNE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):947

An adult female red deer died of a severe seizure and dysbasia. Postmortem computed tomography (CT) and magnetic resonance imaging (MRI) were performed. On CT, deciduous right maxillary second and third premolar teeth were observed, and the right infraorbital canal was disrupted. MRI showed that the right trigeminal nerve was enlarged and the right subarachnoid cavity was occupied by fluid and gas. On gross examination, the right paranasal sinus, swollen muscles of the orbit and tonsils, right trigeminal nerve, and right cerebrum surface contained a yellowish-white, cheese-like pus. Based on these findings, the deer was believed to have developed pyogenic meningitis caused by a neuropathic infection secondary to periodontogenic paranasal sinusitis.  相似文献   

Recombinant plant dsRNA-binding protein as an effective tool for the isolation of viral replicative form dsRNA and universal detection of RNA viruses     

Kappei Kobayashi  Reiko Tomita  Masaru Sakamoto 《Journal of General Plant Pathology》2009,75(2):87-91

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引种日本落叶松原生种源及优树半同胞家系苗期试验初报   总被引：7，自引：6，他引：1  

宋丛文  周必成  荒井赞  伊藤辉胜  永田义明  侯义梅  陈柏如 《湖北林业科技》2000,(Z1)

引种日本落叶松原生种源及优树半同胞家系苗期试验研究结果表明：原生种源问高生长具有明显差异,确定松本、伊那、日光为优良种源;家系间的高生长也具有明显差异,确定县诹访１号等２０个家系为优良家系;原生种源的遗传变异幅度极大,具有相当的遗传改良潜力和遗传资源保存价值。  相似文献   

Sulfoxidation of thiocarbamate herbicides and metabolism of thiocarbamate sulfoxides in living mice and liver enzyme systems     

John E. Casida  Ella C. Kimmel  Hideo Ohkawa  Reiko Ohkawa 《Pesticide biochemistry and physiology》1975,5(1):1-11

The microsome-NADPH system of mouse liver oxidizes each of benthiocarb, butylate, cycloate, EPTC, molinate, pebulate, and vernolate herbicide chemicals to the corresponding thiocarbamate sulfoxide which is then cleaved by the liver soluble-glutathione system. These sulfoxides are also detected as transient metabolites in the liver of mice injected with EPTC, molinate, pebulate, and vernolate but not with the other three thiocarbamates. Thiocarbamate sulfones are not detected as metabolites of the thiocarbamates. Studies in vivo and in vitro with [¹⁴C]EPTC and -pebulate or their corresponding sulfoxides and/or sulfones further indicate that sulfoxidation is the initial metabolic step in cleavage of the thiocarbamate ester group. Sulfoxidation appears to be a detoxification mechanism for thiocarbamate herbicides in mammals.  相似文献   

Cloning,expression, and tissue distribution of bovine interleukin-21   总被引：3，自引：0，他引：3  

Muneta Y  Kikuma R  Yoshihara K  Mori Y 《Veterinary immunology and immunopathology》2003,95(1-2):73-80

Bovine interleukin-21 (IL-21) cDNA was cloned and sequenced from bovine peripheral blood lymphocytes (PBLs) stimulated with 10 microg/ml concanavalin A (ConA), 10 microg/ml phytohemagglutinin (PHA), and 50 ng/ml phorbol 12-myristate 13-acetate (PMA) for 48 h. The open reading frame of the bovine IL-21 cDNA is 459 bp in length and encodes 152 amino acids. The predicted amino acid sequence is 78.2 and 58.5% homologous to the human and murine IL-21 amino acid sequences, respectively. Recombinant bovine IL-21 was expressed by a baculovirus expression system. The bovine IL-21 was processed to the mature form in insect cells and secreted to the supernatant confirmed by N-terminal amino acid sequencing. The recombinant bovine mature IL-21 induced the proliferation of human IL-2-dependent cells, ILT-MAT. The mRNA expression for bovine IL-21 was observed in the spleen, but not in the brain, heart, lung, liver, and kidney. The bovine IL-21 identified in this study may provide new methods for the enhancement of innate immunity in cows.  相似文献   

High level expression and purification of bioactive bovine interleukin-18 using a baculovirus system   总被引：9，自引：0，他引：9  

Nagata T  Ishikawa S  Shimokawa E  Kamikawa M  Kikuma R  Muneta Y  Yokomizo Y  Nakamura M  Takehara K 《Veterinary immunology and immunopathology》2002,87(1-2):65-72

Bioactive recombinant bovine interleukin-18 (rboIL-18) was expressed using a baculovirus system. Normally, IL-18 is translated as a precursor form of a 24kDa polypeptide and processed by IL-1beta converting enzyme (ICE) to a mature bioactive form of 18kDa protein. Hence, to express active form IL-18, we constructed two recombinant baculoviruses containing boIL-18 and human ICE (hICE) genes, respectively, and superinfected these viruses into insect cells. Superinfection of both recombinant viruses into the cells resulted in the expression of a 24kDa precursor form and an 18kDa mature form detectable in the supernatant by immunoblotting using anti-porcine IL-18 antibody. Culture supernatant from the superinfected cells showed a synergistic effect with recombinant boIL-12 for production of interferon-gamma (IFN-gamma) in bovine peripheral mononuclear cells. By addition of histidine hexamer at the C-terminal of boIL-18, the mature IL-18 was purified. Bioactivity remained after purification.  相似文献