Generation of multinucleated giant cells in vitro from bovine monocytes and macrophages     

Yoshihara K  Nagata R  Muneta Y  Inumaru S  Yokomizo Y  Mori Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(9):1065-1069

The generation of multinucleated giant cells (MGC) from cells of the bovine monocyte-macrophage lineage was investigated. Freshly isolated monocytes were incubated with the conditioned medium (CM) of peripheral blood mononuclear cell cultures treated with Concanavalin A for 1-4 days (CM1 to CM4). Only CM1 generated MGC despite similar concentrations of IFNgamma in all CMs. Nevertheless, MGC formation from monocytes was enhanced by adding either macrophage colony-stimulating factor (M-CSF) or granulocyte-macrophage colony-stimulating factor (GM-CSF), MGC formations from macrophages were observed only when macrophages were cultured with GM-CSF plus CM. These results indicate that several mechanisms to generate MGC from bovine monocytes-macrophage lineage cells exist, and that GM-CSF is a major mediator of MGC formation in cattle.  相似文献   

Isolation of foot-and-mouth disease virus from Japanese black cattle in Miyazaki Prefecture, Japan, 2000     

Sakamoto K  Kanno T  Yamakawa M  Yoshida K  Yamazoe R  Murakami Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(1):91-94

  相似文献   

Differences in weed vegetation in response to cultivating methods and water conditions in rainfed paddy fields in north-east Thailand     

SHINSUKE TOMITA  EIJI NAWATA  YASUYUKI KONO  YOSHIKATSU NAGATA  CHAIRAT NOICHANA  AKKADET SRIBUTTA  TATSUYA INAMURA 《Weed Biology and Management》2003,3(2):117-127

Rainfed paddy cultivation predominates in north-east Thailand. Direct dry seeding is replacing transplanting rice cultivation to increase the frequency of successful plantings and to save labor. The present study clarified differences in weed vegetation of direct dry-seeded and transplanted paddy fields under rich, medium and poor water conditions from agricultural and ecological viewpoints. A large number of paddy fields from a wide range of climatic, topographic, soil and hydrological conditions were used. A survey was conducted every three weeks, and data regarding paddy cultivation, weeds and water conditions were collected. Expansion of direct dry seeding may increase species with in-between water adaptability to hygrophytes and mesophytes, and decrease hydrophytes and mesophytes. This characteristic was remarkable under poor water conditions. Rice cover was similar in either planting method under rich water conditions. However, cover of direct-seeded rice was significantly smaller than that of transplanted rice under medium and poor water conditions. Species diversity was higher in direct dry-seeded paddy fields than in transplanted paddy fields under medium and poor water conditions. Impact of direct dry seeding in the fields with rich water conditions on the vegetation is small in the context of maintaining rice production by transplanting and species diversity. However, direct dry seeding is mainly adopted in fields with poor water conditions. Impact of the changes in cultivating methods is large on rice productivity, species composition and species diversity under such conditions. Therefore, how to best use resource-poor fields may be the key to maintaining rice production and regional-level species diversity.  相似文献   

An outbreak of chlamydiosis in captive blue-fronted Amazon parrots (Amazona aestiva) in Brazil.     

Tania de Freitas Raso  Sílvia Nery Godoy  Liliane Milanelo  Cláudia Almeida Igayara de Souza  Eliana Reiko Matuschima  Jo?o Pessoa Araújo Júnior  Aramis Augusto Pinto 《Journal of zoo and wildlife medicine》2004,35(1):94-96

Fifty-eight blue-fronted Amazon parrot (Amazona aestiva) nestlings, recovered from the illegal trade, became ill at a wildlife rehabilitation center in S?o Paulo State, Brazil. Clinical signs observed were nonspecific, and the mortality rate was 96.5% despite initial treatment with norfloxacin. Postmortem examinations were performed on 10 birds. Liver and spleen smears showed structures suggestive of Chlamydophila psittaci in four cases. Diagnosis was confirmed by seminested polymerase chain reaction on tissue samples. Other birds from the same location showed no clinical signs of the disease, although high complement fixation titers to C. psittaci were found in 10 adult psittacines. All birds in the facility were treated with doxycycline. The two surviving nestlings did not recover after two doxycycline treatments and were euthanatized. The high mortality rate observed in this outbreak was attributed to poor conditions of husbandry and delays in the diagnosis and treatment of the disease. After diagnosis, improved control measures for chlamydiosis were instituted.  相似文献   

Imatinib mesylate treatment in a dog with gastrointestinal stromal tumors with a c-kit mutation     

Mitsuhiro IRIE  Yoshinori TAKEUCHI  Yuzo OHTAKE  Hitomi SUZUKI  Nao NAGATA  Takuma MIYOSHI  Yumiko KAGAWA  Tetsushi YAMAGAMI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1535-1539

A 13-year-old spayed mixed-breed dog was diagnosed with a gastrointestinal stromal tumor (GIST) after histopathological examination of an abdominal mass. Five months after surgical resection of the tumor, we detected the recurrence of GIST with multiple disseminated abdominal lesions. A sequence analysis of cDNA obtained from a biopsy of the recurrent tumors revealed a mutation within exon 9 of the c-kit gene (1523A>T, Asn⁵⁰⁸Ile), which has been shown to cause ligand-independent phosphorylation of the KIT protein in GISTs and canine mast cell tumors (MCTs). Upon detection of the recurrent tumors, we initiated treatment with imatinib mesylate (10 mg/kg, q 24 hr). After 2 months, the dog achieved complete remission. Our findings indicate that canine GIST, and possibly MCT, may be responsive to molecular-targeted therapy.  相似文献   

A canine case of otitis media examined and cured using a video otoscope     

Reiko USUI  Yuki OKADA  Emiko FUKUI  Atsuhiko HASEGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(2):237-239

Otitis media of the left ear was diagnosed by video otoscopic examination in a 7-year-old, intact male Shih-tzu dog (weight, 5.1 kg), that also had three complex ceruminous adenomas and a Pseudomonas aeruginosa infection in the left ear canal. In such cases, total ear canal ablation is usually required. However, a complete cure was achieved in the present case without total ear canal ablation. The complex ceruminous adenomas were excised using a diode laser, and repeated cleansing of the tympanic cavity and ear canal was implemented using a video otoscope. As a result, the ear canal was closed in a U-form, and the otitis media was cured.  相似文献   

New Azaphilones,Seco-Chaetomugilins A and D,Produced by a Marine-Fish-Derived Chaetomium globosum     

Takeshi Yamada  Yasuhide Muroga  Reiko Tanaka 《Marine drugs》2009,7(2):249-257

Seco-chaetomugilins A and D were isolated from a strain of Chaetomium globosum that was originally isolated from the marine fish Mugil cephalus, and their absolute stereostructures were elucidated on the basis of spectroscopic analyses, including 1D and 2D NMR techniques, along with the chemical transformation from known chaetomugilins A and D. Seco-chaetomugilin D exhibited growth inhibitory activity against cultured P388, HL-60, L1210, and KB cells.  相似文献   

Design and storage stability of reference materials for microfluidic quantitative PCR-based equine gene doping tests     

Teruaki TOZAKI  Aoi OHNUMA  Mio KIKUCHI  Taichiro ISHIGE  Hironaga KAKOI  Kei-ichi HIROTA  Kanichi KUSANO  Shun-ichi NAGATA 《Journal of Equine Science》2021,32(4):125

One method of gene doping in horseracing is administering of exogenous genetic materials, known as transgenes. Several polymerase chain reaction (PCR)-based methods have been developed for detecting transgenes with high sensitivity and specificity. However, novel designs for reference materials (RMs) and/or positive template controls (PTCs) are necessary for simultaneous analysis of multiple transgene targets. In this study, we designed and developed a novel RM for simultaneously detecting multiple targets via microfluidic quantitative PCR (MFQPCR). Twelve equine genes were selected as targets in this study. A sequence region including primers and probes for quantitative PCR was designed, and a 10 bp sequence was inserted to allow the RM to be distinguished from the original transgene sequences. The sequences of individual detection sites were then connected for 12 genes and cloned into a single plasmid vector. We performed fragment size analysis to distinguish between the PCR products of the original transgene sequence and those of the RM, enabling identification of RM contamination. PTCs diluted to 10,000, 1,000, 100, and 10 copies/µl with horse genomic DNA from RM were stably stored at 4°C for 1 year. As digital PCR enabled absolute quantification, the designed substances can serve as an RM. These findings indicate that the RM design and storage conditions were suitable for gene doping tests using MFQPCR.  相似文献   

Microbial and chemical properties of <Emphasis Type="Italic">aji-no-susu</Emphasis>, a traditional fermented fish with rice product in the Noto Peninsula,Japan     

Takashi Kuda  Reiko Tanibe  Mayumi Mori  Harumi Take  Toshihide Michihata  Toshihiro Yano  Hajime Takahashi  Bon Kimura 《Fisheries Science》2009,75(6):1499-1506

Aji-no-susu is a Japanese fermented fish product prepared from salted horse mackerel Trachurus japonicus, and cooked rice. We studied the organic acid and free amino acid contents and microflora in 12 aji-no-susu products to clarify their features as a lactic-acid-fermented food. Salinity of the samples was approximately 7.0% (rice portion) and 6.0% (fish portion) (w/w). Water activity was approximately 0.9, and pH was approximately 4.4 and lower. In the rice portions, lactic acid content was very high (57 mg/g sample). The predominant amino acids were alanine (2.3 mg/g rice portion) and lysine (2.1 mg/g). In the case of long-fermented (4 and 12 months) aji-no-susu, a high content of gamma-aminobutyric acid (GABA, 1.5 and 1.4 mg/g) was detected. Total viable counts in rice and fish portions were 7.7 and 7.4 log colony-forming units (cfu)/g, respectively. The number of lactobacilli in the rice and fish portions was 7.3 and 7.1 log cfu/g, respectively. Yeasts were detected in eight samples. Furthermore, acid-tolerant lactic acid bacteria (LAB) (Lactobacillus plantarum), GABA-producing LAB (Lactobacillus sp.), and halophilic or halo-tolerant yeast (Debaryomyces hansenii) were isolated and identified. Results in this study indicate that aji-no-susu is a typical traditional lactic-acid-fermented fish product.  相似文献   

Growth hormone secretion during longterm incubation of the pituitary of the Japanese eel,Anguilla japonica     

Reiko Suzuki  Mitsuyo Kishida  Tetsuya Hirano 《Fish physiology and biochemistry》1990,8(2):159-165

Growth hormone (GH) secretion from organ-cultured pituitaries of the eel (Anguilla japonica) was studied during incubation in a defined medium for 2 weeks, using a homologous radioimmunoassay which does not distinguish between the two molecular forms of eel GH. The total amount of GH secreted increased gradually during the incubation period; so that the amount of GH released on day 14 was about 30 times greater than that on day 1. On day 14, the proportion of GH released relative to the total amount of GH present (the sum of GH released into the medium and residual content in the pituitary) was 96% and the amount produced on day 14 was 4 times greater than the content in the unincubated pituitary. Somatostatin (SRIF, 1.8 × 10^-7 M) inhibited the increase in GH release. On day 7, the proportion of GH released by pituitaries treated with SRIF (28%) was less than that released by the control pituitary (91%). There was no significant difference in GH release between the pituitaries incubated in isotonic medium (300 mOsm) and those in hypotonic medium (240 mOsm) for 2 weeks except for the first 3 days, when the pituitaries in hypotonic medium secreted significantly greater amounts of GH than those incubated under isotonic condition. Hypertonic medium (350 mOsm) had no effect on GH release except for significant inhibition on days 6 and 14. When secretion of the two forms of GH (GH I and II) was examined after separation by polyacrylamide gel electrophoresis followed by densitometry, slightly more GH I tended to be secreted than GH II during the culture period, although the effects of SRIF and osmolality of the media on GH I release were similar to those on GH II. It is concluded that GH secretion and production in the eel is mainly under the inhibitory control of hypothalamus, and that osmolality has a minimum influence on the GH release.  相似文献