Dear Referees     

Ulrich Förstner  Wim Salomons  Zhihong Xu  Almut Beate Heinrich 《Journal of Soils and Sediments》2007,7(6):432-432

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Environmental quality standards (EQS) applicable to sediment and/or biota   总被引：1，自引：1，他引：1  

Ulrich Förstner 《Journal of Soils and Sediments》2007,7(4):270-270

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Einfluß steigender Stickstoffgaben auf den Kupferernährungszustand von Getreide     

Reiner Krhmer  Burkhard Sattelmacher 《植物养料与土壤学杂志》1997,160(3):385-392

Effect of increasing nitrogen fertilization on copper demand in cereals Pot experiments were conducted with wheat (2 experiments) and spring barley (1 experiment) in a peat soil. Four N and four Cu fertilizer levels were chosen in such a way that, based on grain yield, within each N level there was a Cu deficient and a Cu sufficient treatment. For sufficient Cu supply, Cu level in soil and plant increased with increasing N level. Critical Cu concentrations in aboveground biomass at shooting [μg (g dw)^?1] were 3.5 (lowest N level = N₁) to 7.5 (highest N level = N₄) for grain production of wheat and barley, but for aboveground biomass of wheat at shooting they were only 2.6 (N₁) to 5.8 (N₄). Based on critical Cu concentration at different N levels, critical Cu/N ratios were determined. These were independent from N level, indicating that Cu remobilization in the older leaves is unlikely even at N deficiency. The high critical Cu concentrations at high N levels may be explained by an increased immobilization of Cu. Under Cu deficient conditions, Cu concentration did not increase with increasing N level. However, under Cu sufficient conditions, N concentration and Cu concentration of the plant increased with increasing N level. The increasing Cu uptake with increasing N level may be explained by the release of amines into the root apoplast and the rhizosphere. Particularly under Cu sufficient conditions, these may mobilize Cu by formation of soluble Cu-amine complexes.  相似文献   

Fast and efficient discrimination of the Isotoma viridis group (Insecta: Collembola) by PCR-RFLP     

Ulrich Burkhardt  Juliane Filser   《Pedobiologia》2004,48(5-6):435-443

Identification of collembolan species is generally based on specific morphological characters, such as chaetotaxy and pigmentation pattern. However, some specimens do not match to described characters because these refer to adult specimens, often of one specific sex, or the characters are highly variable in adults (e.g. pigmentation, setae or furcal teeth). Isozymes have frequently assisted species discrimination, and also these may vary with developmental stage or environmental conditions. For identification of single species of the Isotoma viridis group, we present both direct sequencing of the cytochrome oxidase subunit II (COII) gene and a simple DNA-based molecular method.

Five PCR primers amplifying the COII region (717 bp) of the mitochondrial DNA were used. The sequences clearly separated the species I. viridis, I. riparia and I. anglicana, irrespective of colour varieties within the first species. DNA amplification products of different species can also be distinguished by digestion with restriction endonucleases, followed by gel electrophoresis for separation of fragments. This restriction fragment length polymorphism (RFLP), obtained after digestion with the endonucleases TaqI, VspI, MvaI and Bsp143I, revealed specific fragments that separated the three species from each other. Since restriction enzymes are sensitive to single base mutations, we suggest to use a combination of enzymes with at least two species-specific restriction sites when using the RFLP technique. For the I. viridis complex, VspI and Bsp143I appear to be an appropriate combination.  相似文献   

Effects of input management and crop diversity on non-renewable energy use efficiency of cropping systems in the Canadian Prairie     

R.P. Zentner  P. Basnyat  S.A. Brandt  A.G. Thomas  D. Ulrich  C.A. Campbell  C.N. Nagy  B. Frick  R. Lemke  S.S. Malhi  M.R. Fernandez 《European Journal of Agronomy》2011,34(2):113-123

Although producers’ prime objective may be to increase net returns, many are also interested in conserving and enhancing the quality the soil, water and air resources through adopting more environmentally friendly production practices. This study compared non-renewable energy inputs, energy output, and energy use efficiency of nine dryland cropping systems comprised of a factorial combination of three methods of input management [high (HIGH), i.e., conventional tillage plus full recommended rates of fertilizer and pesticides; reduced (RED), i.e., conservation tillage plus reduced rates of fertilizer and pesticides; and organic (ORG), i.e., conventional tillage plus N-fixing legumes and non-chemical means of weed and pest control]; and three crop rotation systems with varying levels of cropping diversity [a fallow-based rotation with low crop diversity (LOW); a diversified rotation using annual cereal, oilseed and pulse grains (DAG); and a diversified rotation using annual grains and perennial forages (DAP)]. The study was conducted over the 1996–2007 period on a Dark Brown Chernozemic soil (Typic Boroll) in the Canadian Prairies. As expected, total direct plus indirect energy input was the highest for the HIGH and RED input treatments (3773 MJ ha^?1 year^?1), and 50% less for ORG management. Most of the energy savings came from the non-use of inorganic fertilizers and pesticides in the ORG management treatments. Further, total energy use was the highest for the DAG treatments (3572 MJ ha^?1 year^?1), and similar but about 18% lower for the DAP and LOW crop diversity treatments compared to DAG. Thus, overall, the HIGH/DAG and RED/DAG systems had the highest energy requirements (4409 MJ ha^?1 year^?1) and ORG/DAP had the lowest (1806 MJ ha^?1 year^?1). Energy output (calorimetric energy content) was typically the highest for the HIGH input treatments (26,541 MJ ha^?1 year^?1), was about 4% less with RED, and 37% less with ORG management. The latter reflected the lower crop yields obtained with organic management. Similarly, energy output was the highest for the DAP treatments (25,008 MJ ha^?1 year^?1), about 5% less for DAG, and 20% less for the LOW crop diversity treatments. The higher energy output with the DAP treatments largely reflected that the entire harvested biomass of the forage crops was included in energy output, while for grain crops only the seed was included. The straw and crop residues from annual crops were returned to the land to protect the soil from erosion and to maintain soil organic matter as this is the recommended practice in this semi-arid region. In contrast to energy output and to net energy produced, energy use efficiency (measured as yield of grain plus forage produced per unit of energy input or as energy output/energy input ratio) was the highest for the ORG input treatments (497 kg of harvested production GJ^?1 of energy input, and an energy output/energy input ratio of 8.8). We obtained lower, but generally similar energy use efficiency for the HIGH and RED input treatments (392 kg GJ^?1 and ratio of 7.1). Thus, overall, ORG/DAP was the most energy efficient cropping system, while RED/LOW and RED/DAG generally ranked the lowest in energy use efficiency. Our findings support the current movement of producers toward ORG management as a means of reducing the reliance on non-renewable energy inputs and improving overall energy use efficiency of their cropping systems. Our results also suggest that moving away from traditional monoculture cereal rotations that employ frequent summer fallowing, toward extended and diversified crop rotations that use reduced tillage methods, although resulting in an increase in energy output, will not significantly reduce the overall reliance on non-renewable energy inputs, nor enhance energy use efficiency, unless perennial legume forages and/or legume grain crops are included in the cropping mix.  相似文献   

Immunology. T cell activation in six dimensions     

von Andrian UH 《Science (New York, N.Y.)》2002,296(5574):1815-1817

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ON THE CHEMICAL ALTERATION OF PURIFIED ANTIBODY-PROTEINS     

Reiner L 《Science (New York, N.Y.)》1930,72(1871):483-484

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Ordering in a fluid inert gas confined by flat surfaces     

Donnelly SE  Birtcher RC  Allen CW  Morrison I  Furuya K  Song M  Mitsuishi K  Dahmen U 《Science (New York, N.Y.)》2002,296(5567):507-510

High-resolution transmission electron microscopy images of room-temperature fluid xenon in small faceted cavities in aluminum reveal the presence of three well-defined layers within the fluid at each facet. Such interfacial layering of simple liquids has been theoretically predicted, but observational evidence has been ambiguous. Molecular dynamics simulations indicate that the density variation induced by the layering will cause xenon, confined to an approximately cubic cavity of volume approximately 8 cubic nanometers, to condense into the body-centered cubic phase, differing from the face-centered cubic phase of both bulk solid xenon and solid xenon confined in somewhat larger (>/=20 cubic nanometer) tetradecahedral cavities in face-centered cubic metals. Layering at the liquid-solid interface plays an important role in determining physical properties as diverse as the rheological behavior of two-dimensionally confined liquids and the dynamics of crystal growth.  相似文献   

Role of T-bet in commitment of TH1 cells before IL-12-dependent selection     

Mullen AC  High FA  Hutchins AS  Lee HW  Villarino AV  Livingston DM  Kung AL  Cereb N  Yao TP  Yang SY  Reiner SL 《Science (New York, N.Y.)》2001,292(5523):1907-1910

How cytokines control differentiation of helper T (TH) cells is controversial. We show that T-bet, without apparent assistance from interleukin 12 (IL-12)/STAT4, specifies TH1 effector fate by targeting chromatin remodeling to individual interferon-gamma (IFN-gamma) alleles and by inducing IL-12 receptor beta2 expression. Subsequently, it appears that IL-12/STAT4 serves two essential functions in the development of TH1 cells: as growth signal, inducing survival and cell division; and as trans-activator, prolonging IFN-gamma synthesis through a genetic interaction with the coactivator, CREB-binding protein. These results suggest that a cytokine does not simply induce TH fate choice but instead may act as an essential secondary stimulus that mediates selective survival of a lineage.  相似文献   

Dynamic visualization of thrombopoiesis within bone marrow     

Junt T  Schulze H  Chen Z  Massberg S  Goerge T  Krueger A  Wagner DD  Graf T  Italiano JE  Shivdasani RA  von Andrian UH 《Science (New York, N.Y.)》2007,317(5845):1767-1770

Platelets are generated from megakaryocytes (MKs) in mammalian bone marrow (BM) by mechanisms that remain poorly understood. Here we describe the use of multiphoton intravital microscopy in intact BM to visualize platelet generation in mice. MKs were observed as sessile cells that extended dynamic proplatelet-like protrusions into microvessels. These intravascular extensions appeared to be sheared from their transendothelial stems by flowing blood, resulting in the appearance of proplatelets in peripheral blood. In vitro, proplatelet production from differentiating MKs was enhanced by fluid shear. These results confirm the concept of proplatelet formation in vivo and are consistent with the possibility that blood flow-induced hydrodynamic shear stress is a biophysical determinant of thrombopoiesis.  相似文献