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91.
Reis EG Gremião ID Kitada AA Rocha RF Castro VS Barros MB Menezes RC Pereira SA Schubach TM 《Journal of Feline Medicine and Surgery》2012,14(6):399-404
Sporotrichosis is a mycosis caused by Sporothrix schenckii. The most affected animal is the cat; it has played an important role in the zoonotic transmission of this disease, especially in Rio de Janeiro, Brazil, since 1998. In order to evaluate the treatment of feline sporotrichosis with potassium iodide, an observational cohort was conducted in 48 cats with sporotrichosis at Instituto de Pesquisa Clínica Evandro Chagas, Fiocruz. All cats received potassium iodide capsules, 2.5 mg/kg to 20 mg/kg q24h. The cure rate was 47.9%, treatment failure was 37.5%, treatment abandonment was 10.4% and death was 4.2%. Clinical adverse effects were observed in 52.1% of the cases. Thirteen cats had a mild increase in hepatic transaminase levels during the treatment, six of them presented clinical signs suggestive of hepatotoxicity. Compared to previous studies with itraconazole and iodide in saturated solution, potassium iodide capsules are an alternative for feline sporotrichosis treatment. 相似文献
92.
Sousa Andria Gama Pacheco Acácio H. B. Siqueira-Pinto Gilson A. dos Reis Gleika T. J. Fugimura Michelle M. S. Vaz Luciano J. Marcusso Paulo F. Ramos-Espinoza Fernando C. da Silva Claudiano Gustavo 《Aquaculture International》2021,29(3):977-988
Aquaculture International - The objective of the present study was to evaluate the action of benzocaine and eugenol over anesthesia stages and the use of three anticoagulants (ethylenediamine... 相似文献
93.
Diana B. Reis Anastasia Shcherbakova Rodrigo Riera Virgina M. Martín Pedro Domingues Jos P. Andrade Pablo Jimnez‐Prada Covadonga Rodríguez Antnio V. Sykes Eduardo Almansa 《Aquaculture Research》2021,52(1):105-116
To move forward in the farming of Octopus vulgaris paralarvae, it is necessary to search for a live prey easy to obtain and maintain in the laboratory that meets the nutritional requirements of the octopus paralarvae and adapts to its predatory behaviour. Grapsus adscensionis zoeae (Crustacea, Decapoda) seems to fulfil most of these targets, and it was herein used to deepen knowledge of paralarvae lipid requirements and composition, growth and survival. To this purpose, the effects of feeding with Grapsus zoeae as sole prey were compared with Artemia at two different stages (nauplii and juveniles), which also differed in their lipid profiles. After 15 days of feeding, the best growth and survival of paralarvae was obtained in the Grapsus group, and no differences were observed between both Artemia groups. Triacylglycerides storage in paralarvae seemed to be co‐related with a lower growth and survival, but not with its prey levels. Contrarily, sterol ester levels were higher in paralarvae fed Grapsus, reflecting its content in the prey. The best paralarval viability was related to higher levels of 22:6n‐3 (DHA) and 20:4n‐6 (ARA), also reflecting its higher content in the prey. On the other hand, neither the 20:5n‐3 (EPA) levels in the prey nor in paralarvae were related to growth or survival. The implications of these results are discussed considering the lipid requirements of O. vulgaris paralarvae. 相似文献
94.
Priscilla F. Gerber Grazielle C.F. Galinari Marcos X. Silva Fbia S. Campos Adrienny C.T. Reis Zlia I.P. Lobato 《Research in veterinary science》2009,87(3):488-491
A comparative serologic study was performed in seven single site (SS) farrow-to-finish farms and four multi-site (MS) farrow-to-finish farms, with or without post-weaning multisystemic wasting syndrome (PMWS). In each farm, 30 blood samples were collected for each category of the production cycle: sows, farrowing crate, nursery, grower pigs, and finishing pigs. Sera were evaluated for the presence of antibodies to porcine circovirus type-2 (PCV2) via immunoperoxidase monolayer assay. Serologic profiles for PCV2 were different between SS and MS farms. Seroconversion following the decline in maternal antibodies occurred at a later stage on SS farms (grower pigs) than MS farms (nursery pigs). MS farms tended to have lower antibody titers than SS farms in the categories of sow, piglet, and nursery, while higher antibody titers were found in grower pigs. Characterization of serologic profiles for different farms may provide important information for the adoption of vaccination programs. 相似文献
95.
Detection of porcine Circovirus type 2 (PCV2) variants PCV2-1 and PCV2-2 in Brazilian pig population
Janice Reis Ciacci-Zanella Luciano S. Pinto Lana Teixeira Fernandes Odir Antonio Dellagostin 《Research in veterinary science》2009,87(1):157-232
In the present study whole genome of six Brazilian isolates of PCV2 were sequenced, analyzed and compared with 35 other sequences (24 from other countries and 17 from Brazil). The phylogenetic analysis showed that mostly Brazilian variants of PCV2 were grouped as PCV2-1. Two isolates among the six analyzed here could not be grouped with any other PCV2-2 analyzed in this study. One of these isolates was from an aborted fetus with myocarditis and the other from a PMWS affected pig. The results pointed here showed that both groups of PCV2 are present in Brazilian pig population without any clear geographical correlation. 相似文献
96.
Liliana de Oliveira Rocha Gabriela Martins Reis Raquel Braghini Estela Kobashigawa Jansen de Araújo Benedito Corrêa 《European journal of plant pathology / European Foundation for Plant Pathology》2012,132(3):353-366
Aflatoxins can cause great economic losses and serious risks to humans and animals health. The largest aflatoxin producers
belong to Aspergillus section Flavi and can occur naturally in food commodities. Studies showed that molecular tools as well as the type of sclerotia produced
by the strains could be helpful for identification of Aspergillus species and could be correlated with levels of toxin production. The purpose of this work was to characterize the genetic
diversity using AFLP technique, the type of sclerotia and the ability of aflatoxin production by isolated strains from corn
of different origins in Brazil, and to verify whether qPCR based on aflR and aflP genes is appropriate for estimating the level of aflatoxin production. All the 75 strains were classified as A. flavus and the AFLP technique showed a wide intraspecific variability within them. Regarding sclerotia production, 34% were classified
as S and 66% as L type. Among the aflatoxin-producers, 52.8% produced aflatoxin B1, while 47.2% aflatoxins B1 and B2. Statistical analysis showed no correlation between sclerotia production and aflatoxigenicty, and no correlation between
the phylogenetic clusters and aflatoxin production. Concerning the relative expression of aflR and aflP, Pearson’s correlation test demonstrated low positive correlation between the expression of the aflR and aflP genes and the production of AFB1 and AFB2, but showed high positive correlation between aflR and aflP expression. In contrast to the other reference strains, A. oryzae ATCC 7282 showed no amplification of aflR and aflP. The results highlight the need for detection of reliable and reproducible markers with a high positive correlation with aflatoxin
production. 相似文献
97.
98.
Cooke RF Cappellozza BI Reis MM Bohnert DW Vasconcelos JL 《Journal of animal science》2012,90(9):3266-3273
Three experiments were conducted to evaluate plasma concentrations of glucose, insulin, IGF-I, and progesterone (P4) in pubertal beef heifers receiving exogenous glucose, insulin, or sometribove zinc. All heifers used had no luteal P4 synthesis but received a controlled internal drug-releasing device containing 1.38 g of P4 to estimate treatment effects on hepatic P4 degradation. In Exp. 1, 8 pubertal, nulliparous Angus × Hereford heifers (initial BW = 442 ± 14 kg; initial age = 656 ± 7 d) were randomly assigned to receive, in a crossover design containing 2 periods of 10 h, intravenous (i.v.) infusions (10 mL) of insulin (1 μg/kg of BW; INS) or saline (0.9%; SAL). Treatments were administered via jugular venipuncture in 7 applications (0.15 μg insulin/kg BW per application) 45 min apart (from 0 to 270 min). Blood samples were collected immediately before each infusion as well as at -120, -60, 330, 390, and 450 min relative to the first infusion. Heifers receiving INS had greater (P < 0.01) plasma insulin, reduced (P ≤ 0.04) plasma glucose and IGF-I, and similar (P = 0.62) plasma P4 concentrations compared with SAL heifers. In Exp. 2, the same heifers were assigned to receive, in a similar experimental design as Exp. 1, i.v. infusions (10 mL) of 1) insulin (1 μg/kg BW) and glucose (0.5 g/kg BW; INS+G) or 2) SAL. Heifers receiving INS+G had greater (P ≤ 0.02) plasma insulin, glucose, and P4 but reduced (P = 0.01) plasma IGF-I concentrations compared with SAL heifers. In Exp. 3, the same heifers were assigned to receive, in a crossover design containing 2 periods of 14 d, subcutaneous (s.c.) injections of 1) 250 mg of sometribove zinc (BST) or 2) SAL. Blood samples were collected 3 h apart (0900, 1200, 1500, and 1800 h) from heifers on d 6, 8, and 10 relative to treatment administration (d 1). Heifers receiving BST had greater (P < 0.01) plasma glucose and IGF-I and similar (P ≥ 0.67) plasma insulin and P4 concentrations compared with SAL heifers. Results from this series of experiments suggested that concurrent increases in glucose and insulin are required to reduce hepatic catabolism and increase plasma concentrations of P4 in bovine females. 相似文献
99.
Fujiwara RT Vale AM França da Silva JC da Costa RT Quetz Jda S Martins Filho OA Reis AB Corrêa Oliveira R Machado-Coelho GL Bueno LL Bethony JM Frank G Nascimento E Genaro O Mayrink W Reed S Campos-Neto A 《Veterinary research》2005,36(5-6):827-838
Control of canine visceral leishmaniasis (VL) remains a difficult and serious problem mostly because there is no reliable and effective vaccine available to prevent this disease. A mixture of three recombinant leishmanial antigens (TSA, LeIF and LmSTI1) encoded by three genes highly conserved in the Leishmania genus have been shown to induce excellent protection against infection in both murine and simian models of cutaneous leishmaniasis. A human clinical trial with these antigens is currently underway. Because of the high degree of conservation, these antigens might be useful vaccine candidates for VL as well. In the present study, using the dog model of the visceral disease, we evaluated the immunogenicity of these three antigens formulated with two different adjuvants, MPL-SE and AdjuPrime. The results were compared with a whole parasite vaccine formulated with BCG as the adjuvant. In order to investigate if sensitization with the recombinant antigens would result in recognition of the corresponding native parasite antigens upon infection, the animals were exposed for four weeks after the termination of the immunization protocol with the recombinant antigens to a low number of L. chagasi promastigotes, an etiological agent of VL. Immune response was evaluated by quantitative ELISA in the animal sera before and after exposure to the viable parasites. Both antigen specific IgG1 and IgG2 antibody levels were measured. Immunization of dogs with the recombinant antigens formulated in either MPL-SE or AdjuPrime resulted in high antibody levels particularly to LmSTI1. In addition, this immunization although to low levels, resulted in the development of antibody response to the whole parasite lysate. Importantly, experimental exposure with low numbers of culture forms of L. chagasi promastigotes caused a clear boost in the immune response to both the recombinant antigens and the corresponding native molecules. The boost response was predominantly of the IgG2 isotype in animals primed with the recombinant antigens plus MPL-SE. In contrast, animals primed with the recombinant antigens formulated in AdjuPrime as well as animals vaccinated with crude antigen preparation responded with mixed IgG1/IgG2 isotypes. These results point to the possible use of this antigen cocktail formulated with the adjuvant MPL-SE in efficacy field trials against canine VL. 相似文献
100.
Schubach TM Schubach A Okamoto T Pellon IV Fialho-Monteiro PC Reis RS Barros MB Andrade-Perez M Wanke B 《The Journal of small animal practice》2003,44(9):395-398
The recovery of Sporothrix schenckii from blood samples is rare, and the diagnosis of systemic sporotrichosis is usually made at necropsy. In this report, S schenckii was isolated from two or more internal organs of nine necropsied cats with naturally acquired sporotrichosis. Haematogenous spread was demonstrated in vivo by the isolation of S schenckii from the peripheral blood of 17 (n = 49, 34.4 per cent) cats. Feline leukaemia virus (FeLV) was not detected, and co-infection with feline immunodeficiency virus (FIV), observed in nine cases (n = 43, 20.9 per cent), apparently did not affect the isolation of S schenckii from peripheral blood or from the internal organs. 相似文献