Effects of Growth Hormone on In Situ Culture of Bovine Preantral Follicles are Dose Dependent          下载免费PDF全文

CR Jimenez  JL de Azevedo  RG Silveira  J Penitente‐Filho  EL Carrascal‐Triana  AM Zolini  VR Araújo  CAA Torres 《Reproduction in domestic animals》2016,51(4):575-584

The objective of this study was to evaluate different concentrations of growth hormone (GH) on the development of bovine preantral follicles cultured included in the ovarian tissue (in situ) on the rates of morphologically normal, viable, primordial and developing follicles, as well as the oocyte and follicle diameter and ultrastructural analysis. Ovarian fragments collected from cows with no cross‐breeds defined were cultured in situ for 1 and 7 days in minimal essential medium (α‐MEM+) supplemented with different concentrations of recombinant human GH (0, 10, 25, 50 ng/ml). The ovarian fragments non‐cultured (control) and cultured were processed for classic histology, mechanical isolation and electron transmission microscopy (MET). The parameters underwent anova (Tukey′s and Dunnett′s tests) and chi‐square test (χ²). After 7 days of culture, the treatment with 50 ng/ml GH showed no differences with fresh control (p > 0.05) and had greater effectiveness than in the 0, 10 and 25 ng/ml GH concentrations of the morphologically normal follicles. Regarding the primordial follicles, a reduction was observed in the 50 ng/ml GH concentration concomitant with the significant increase in developing follicles, differing from both the fresh control and the other GH concentrations tested. In addition, 50 ng/ml GH showed a larger follicle and oocyte diameter when compared to the other treatments cultured. Similar structures were ultrastructurally observed in the control group, 50 ng/ml GH. Follicles cultured in 10 ng/ml GH showed nuclear invagination, vacuoles and lesioned basal membrane. Hence, it is concluded that 50 ng/ml GH is the most effective concentration for the development of preantral follicles cultured in situ.  相似文献   

Aboveground biomass production of a semi-arid southern African savanna: towards a new model     

Anthony R Palmer  Igshaan Samuels  Clement Cupido  Andiswa Finca  W Fransiska Kangombe  Isa AM Yunusa 《African Journal of Range and Forage Science》2016,33(1):43-51

Following a field campaign to determine the species composition, canopy cover, aboveground annual production and leaf area index (LAI) of the semi-arid savanna of north-western Namibia, we present a production model that can be used by graziers to determine the livestock carrying capacity. The model predicts the annual aboveground net primary production (ANPP) from regression equations of canopy cover by annual production fraction for plant functional classes. We tested the output of the model against another fully independent net primary production (NPP) model, namely the MODIS NPP product. The mean MODIS NPP for the 29 sites was 343 ± 22?kg dry matter (DM) ha^?1 y^?1 as opposed to 285 ± 142?kg DM ha^?1 y^?1 for the fAP model that used the regression method (p < 0.01). As a proof of concept, these landscape-scale ANPP values are used to calculate a recommended livestock carrying capacity for the Ehirovipuka Communal Conservancy, a 1 980 km² communal area with both wildlife and livestock populations. In addition, we also provide details of a field method for predicting landscape-scale LAI from line transect data. This approach can be used to ground reference the LAI values generated from the MODIS LAI product.  相似文献   

Diagnosis and ultrasonographic appearance of hepatic metastasis in six cases of canine appendicular osteosarcoma (2005–2013)          下载免费PDF全文

L Cesario  LD Garrett  AM Barger  RT O'Brien  TM Fan 《Australian veterinary journal》2016,94(5):160-165

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Negative pressure wound therapy using a portable single‐use device for free skin grafts on the distal extremity in seven dogs          下载免费PDF全文

AJ Miller  RG Cashmore  AM Marchevsky  M Havlicek  PM Brown  SM Fearnside 《Australian veterinary journal》2016,94(9):309-316

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Obtaining Wisent early blastocyst in vitro is a basic for protection and creation of biodiversity for this threatened species          下载免费PDF全文

AM Duszewska  P Gręda  M Baraniewicz  W Bielecki  W Niżański  A Partyka  M Tracz  Z Nowak  A Chełmońska‐Soyta  W Olech 《Reproduction in domestic animals》2018,53(3):818-821

Wisent, or European bison (Bison bonasus), is listed as “vulnerable” on the IUCN Red List of Threatened Species and is therefore protected by international law. For the first time, a Wisent embryo has been obtained in vitro. This procedure creates a new opportunity to protect and increase Wisent reproductive potential and thereby opens new possibilities for the establishment of a controlled and broad reserve of the gene pool.  相似文献   

Testing the unifying theory of ozone sensitivity with mature trees of Fagus sylvatica and Picea abies     

Nunn AJ  Weiser G  Reiter IM  Häberle KH  Grote R  Havranek WM  Matyssek R 《Tree physiology》2006,26(11):1391-1403

The broad range in plant responses to chronic O(3) exposure compels a search for integrative, underlying principles. One such approach is the unifying theory proposed by Reich (1987), which combines the O(3) response of contrasting physiognomic classes of plants on the basis of their intrinsic leaf diffusive conductance and, hence, capacity for O(3) uptake. Physiognomic classes differ in the proportional decline in photosynthesis and growth when compared on the basis of cumulative O(3) exposure per unit time, but converge when compared on the basis of O(3) uptake per unit time or cumulative O(3) uptake over the entire lifetime of the leaf. The theory is based on observations on a large number of contrasting plant species, relying primarily on studies of juvenile trees subjected to short-term O(3) exposure. To test the applicability of the unifying theory to mature trees, broadleaf deciduous European beech (Fagus sylvatica L.) and the evergreen conifer Norway spruce (Picea abies (L.) Karst.) in a mature mixed stand were exposed to either ambient air (control) or air with twice the ambient O(3) concentration delivered into the canopy by means of a free-air fumigation system. We accounted for differences in growing season length, leaf longevity and O(3)-related effects on leaf diffusive conductance in determining total O(3) uptake over the lifetime of the leaf. On this basis, Norway spruce needles required 5 years to take up as much O(3) as did beech leaves in one growing season. The core of the unifying theory on O(3) sensitivity was substantiated in relation to O(3) exposure and uptake. However, contrary to the unifying theory, which was formulated on the basis of results with juvenile trees, the O(3) response of mature trees in a natural stand was more complex. The increased complexity was attributed to additional environmental stressors, stress compensation at the whole-tree level, and differential O(3) sensitivities of leaves according to age class and position within the canopy. Contrary to the theory, photosynthesis was no less sensitive to O(3) in Norway spruce than that of beech, and was reduced in the twice-ambient O(3) regime in the first year of exposure.  相似文献   

Scanning Electron Microscopy of the Orbital Harderian Gland in the Male Atlantic Bottlenose Dolphin (Tursiops truncatus)     

G. G. Ortiz  A. Feria-Velasco  F. P. Pacheco-Moisés  S. Rodríguez-Reinoso  J. A. Cruz-Ramos  S. A. Rosales-Corral    R. J. Reiter 《Anatomia, histologia, embryologia》2009,38(4):279-281

The ultrastructure of the Harderian gland of Atlantic bottlenose dolphin ( Tursiops truncatus ) was examined by scanning electron microscopy (SEM). We found the following surface features: the typical round appearance of the ascinar glandular unit with a finely granular surface, a thin cortex and immediately below two types of cells: type I cells (characterized by small lipid vacuoles) and type II cells (characterized by large lipid vacuoles). It has been suggested that different cells forms represent a single cell type in varying activity states. Additionally, a coalescent tubular complex, a small balloon-like structures and large globular structures were observed. These structures may be reservoirs of secretion products.  相似文献   

Efficiency of Two Enucleation Methods Connected to Handmade Cloning to Produce Transgenic Porcine Embryos     

J Li  K Villemoes  Y Zhang  Y Du  PM Kragh  S Purup  Q Xue  AM Pedersen  AL Jørgensen  JE Jakobsen  L Bolund  H Yang  G Vajta 《Reproduction in domestic animals》2009,44(1):122-127

The purpose of our work was to establish an efficient-oriented enucleation method to produce transgenic embryos with handmade cloning (HMC). After 41–42 h oocytes maturation, the oocytes were further cultured with or without 0.4 μg/ml demecolcine for 45 min [chemically assisted handmade enucleation (CAHE) group vs polar body (PB) oriented handmade enucleation (OHE) group respectively]. After removal of the cumulus cells and partial digestion of the zona pellucida, oocytes with visible extrusion cones and/or polar bodies attached to the surface were subjected to oriented bisection. Putative cytoplasts without extrusion cones or PB were selected as recipients. Two cytoplasts were electrofused with one transgenic fibroblasts expressing green fluorescent protein (GFP), while non-transgenic fibroblasts were used as controls. Reconstructed embryos were cultured in Well of Wells (WOWs) with porcine zygote medium 3 (PZM-3) after activation. Cleavage and blastocyst rates were registered on day 2 and day 7 of in vitro culture respectively. Meanwhile, the total blastocyst cell number was counted on day 7. We found that the difference was only observed between blastocyst rates (38.6 ± 2% vs 48.1 ± 3%) of cloned embryos with GFP transgenic fibroblast cells after CAHE vs OHE. With adjusted time-lapse for zonae-free cloned embryos cultured in WOWs with PZM-3, it was obvious that in vitro developmental competence after CAHE was compromised when compared with the OHE method. OHE enucleation method seems to be a potential superior alternative method used for somatic cell nuclear transfer (SCNT) with transgenic fibroblast cells.  相似文献   

Repair of a gingival cleft associated with a maxillary canine tooth in a dog     

Rawlinson JE  Reiter AM 《Journal of veterinary dentistry》2005,22(4):234-242

This case report describes repair of a gingival cleft located on the labial aspect of the maxillary left canine tooth in a client-owned dog. Multiple procedures were performed including elevation of a pedicle flap, granulation tissue removal, alveoloplasty and root planing, and placement of an osteoconductive bone graft material. The surgical site was closed with a laterally repositioned flap. Postoperative examinations were performed at 2 and 3-weeks without chemical restraint and at 3 and 10-months postoperatively under general anesthesia. At the 10-month postoperative examination, a broad band of gingiva surrounded the maxillary left canine tooth, and probing revealed absence of periodontal pockets. Intraoral dental radiographs indicated maintenance of alveolar bone height and incorporation of the bone graft material into alveolar bone.  相似文献   

Graduates in Veterinary Science     

Martin AM  Currie E. 《Australian veterinary journal》2004,82(8):496-496

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