Pregnancy‐Associated Glycoprotein Secretion in North Moroccan Goats     

M Chentouf  B El Amiri  J Sulon  JF Beckers  N Kirschvink  B Boulanouar  JL Bister 《Reproduction in domestic animals》2008,43(6):696-700

The plasma profile of pregnancy‐associated glycoprotein (PAG) and its relationship with fetal number were studied in 14 North Moroccan goats using a semi‐heterologous radioimmunoassay (RIA). Peripheral blood was collected every day during the first month of pregnancy, afterward the blood samples were collected trice a week. The PAG were first detected at day 20 of pregnancy, their levels increase to week 8 of gestation then decrease slightly until parturition. Statistical differences between goats carrying one or two foetuses are observed from week 5 of pregnancy until parturition. Factorial Discriminant Analysis provides mathematical models for the discrimination between the litter size using the PAG level and the week of pregnancy. Using only one blood sample per week, high sensitivity, specificity and overall accuracy (74%, 88% and 81%) were obtained using these models. It is concluded that the PAG RIA is an effective tool for early diagnosis of pregnancy and for discrimination between the litter sizes in North Moroccan goats.  相似文献   

Effect of Sperm Cryopreservation on the European Eel Sperm Viability and Spermatozoa Morphology     

JF Asturiano  F Marco-Jiménez  DS Peñaranda  DL Garzón  L Pérez  JS Vicente  M Jover 《Reproduction in domestic animals》2007,42(2):162-166

The main objective of the present work was to study the effect of cryopreservation of European eel sperm both on the sperm viability and the spermatozoa head morphology. Spermatozoa morphology was evaluated with computer-assisted morphology analysis after collection in fresh samples, after adding the freezing medium containing dimethyl sulfoxide as cryoprotectant and, finally, after the cryopreservation process and thawing. Cell viability was assessed, in both fresh and thawed samples, by Hoechst 33258 staining. Computer-assisted sperm analysis (CASA) was used to determine the percentage of motile cells and to measure motility parameters in sperm samples. A significant decrease of head perimeter (12.56%) and area (17.90%) was detected from spermatozoa in fresh to thawed samples, indicating that cells do not recover the original size after the cryopreservation process. CASA was used to measure the percentage of motile cells (51.9%) and spermatozoa motility parameters such as curvilinear, straight line and angular path velocities, as well as beating cross frequency. This technique was employed in the fresh sperm samples but proteins present at the freezing medium (L-alpha-phosphatidylcholine) made impossible to use this last technique in thawed samples. When sperm viability was assessed by Hoechst staining, a significant decrease of approximately 15% (73.10 vs 58.26%) of alive spermatozoa was registered from fresh to thawed samples. The percentage of motile cells measured by CASA in fresh samples (51.9%) was lower than the percentage of alive cells determined by Hoechst stainning, suggesting the existence of different batches of spermatozoa in different stages of development, even during the eight to tenth weeks of treatment, when the highest sperm quality was found.  相似文献   

Protein and esterase patterns of two formae speciales of Fusarium oxysporum   总被引：1，自引：0，他引：1  

J A Meyer  J L Renard 《Phytopathology》1969,59(10):1409-1411

  相似文献   

谷子新品种朝谷11号特征特性及高产栽培技术     

孙佳馥  赵术伟 《辽宁农业科学》2000,(4):53-54

朝谷 1 1号由辽宁省水土保持研究所选育 ,于1 997年经辽宁省品种审定委员会审定通过。1 998年被列为省重点农技推广项目 ,计划在 1 998～ 2 0 0 0年在辽宁省朝阳市、阜新市、葫芦岛市的 8县 2区及赤峰市的宁城县推广 6.7万ｈｍ2 。1　朝谷 11号主要特征特性1 .1　农艺性状朝谷 1 1号的叶片、成株茎均为绿色 ,苗期生长快 ,株型紧凑整齐 ,株高 1 65ｃｍ左右 ,穗长 2 3～ 2 5ｃｍ ,单穗粒重 1 4～ 1 6ｇ ,籽粒圆形、黄色 ,出谷率 83%～ 84%,谷草比 1∶1 .3。1 .2　生育期及抗性朝谷 1 1号为中早熟品种 ,生育期 1 0 4～ 1 1 4ｄ ,4月下旬至 5月…  相似文献   

NEAR's flyby of 253 mathilde: images of a C asteroid     

J Veverka  P Thomas  A Harch  B Clark  JF Bell  B Carcich  J Joseph  C Chapman  W Merline  M Robinson  M Malin  LA McFadden  S Murchie  SE Hawkins  R Farquhar  N Izenberg  A Cheng 《Science (New York, N.Y.)》1997,278(5346):2109-2114

On 27 June 1997, the Near Earth Asteroid Rendezvous (NEAR) spacecraft flew within 1212 kilometers of asteroid 253 Mathilde. Mathilde is an irregular, heavily cratered body measuring 66 kilometers by 48 kilometers by 46 kilometers. The asteroid's surface is dark (estimated albedo between 0.035 and 0.050) and similar in color to some CM carbonaceous chondrites. No albedo or color variations were detected. The volume derived from the images and the mass from Doppler tracking of the spacecraft yield a mean density of 1.3 +/- 0.2 grams per cubic centimeter, about half that of CM chondrites, indicating a porous interior structure.  相似文献   

Flavonols and anthocyanins of bush butter, Dacryodes edulis (G. Don) H.J. Lam, fruit. changes in their composition during ripening     

Ella Missang C  Guyot S  Renard CM 《Journal of agricultural and food chemistry》2003,51(25):7475-7480

Dacryodes edulis is a multipurpose tree presently undergoing domestication in central Africa and the countries bordering the Gulf of Guinea, the fruits of which are a good source of essential fatty acids. Polyphenols were characterized in the skin zone and in the pulp of bush butter [Dacryodes edulis (G. Don) H.J. Lam] fruits at different stages of ripeness, from unripe to soft fruits. Total polyphenols, assayed according to the Folin-Ciocalteu method, accounted for 3.0-4.2 mg/g of fresh skin, corresponding to 18.6-21.6 mg/g of defatted dry skin, higher concentrations than in the pulp, with 1.1-1.4 mg/g of fresh weight, corresponding to 5.4-12.3 mg/g of defatted dry weight. Reversed-phase high-performance liquid chromatography revealed the presence of flavonols and anthocyanins. Flavonols appeared to be the main class, with quercitrin as the main individual compound, and the highest concentrations occurred in the skin zone. Hyperin, isoquercitrin, isorhamnetin rhamnoside, and isorhamnetin hexoside were also present in relatively high amounts. Petunidin, cyanidin, and peonidin hexosides were identified by mass spectrometry. In the course of ripening, the total polyphenols as well as the major flavonols increased slightly between unripe and preripe stages and then declined gradually as ripening progressed. Anthocyanin profiles also showed a substantial change during ripening, concomitant with the color change from pink to purple.  相似文献   

Composition of phenolic compounds in a Portuguese pear (Pyrus communis L. var. S. Bartolomeu) and changes after sun-drying     

Ferreira D  Guyot S  Marnet N  Delgadillo I  Renard CM  Coimbra MA 《Journal of agricultural and food chemistry》2002,50(16):4537-4544

The composition of phenolic compounds of a Portuguese pear cultivar (Pyrus communis L. var. S. Bartolomeu) was determined by HPLC after thioacidolysis. The average concentration of phenolic compounds in pear harvested at commercial maturity stage was 3.7 g per kg of fresh pulp. Procyanidins were the predominant phenolics (96%), with a mean degree of polymerization (mDP) of 13-44; hydroxycinnamic acids (2%), arbutin (0.8%), and catechins (0.7%) were also present. The most abundant monomer in the procyanidin structures was (-)-epicatechin (99%), which was found as extension and terminal units; (+)-catechin (1%) was found only as a terminal unit. Sun-drying of these pears caused a decrease of 64% (on a dry pulp basis) in the total amount of native phenolic compounds. Hydroxycinnamic acids and procyanidins showed the largest decrease; the B2 procyanidin was not found at all in the sun-dried pear. Less affected were arbutin and catechins. In the sun-dried pear, the procyanidins with high mDP became unextractable in the solvents used.  相似文献   

Characterization of Brassica nigra chromosomes and of blackleg resistance in B. napus–B. nigra addition lines     

A. M. Chevre    F. Eber    P. This    P. Barret    X. Tanguy    H. Brun    M. Delseny  M. Renard 《Plant Breeding》1996,115(2):113-118

Brassica napus-B. nigra addition lines were previously created using the variety ‘Darmor’ as the oilseed rape genetic background. Two isozyme loci and 46 RAPD markers were added on five different B. nigra chromosomes. The oilseed rape variety used was highly susceptible to blackleg at the cotyledon stage and only the addition of chromosome 4 gave the same level of blackleg resistance as B. nigra. This resistance was efficient whatever the isolates used. A significant effect on the development of stem canker under field conditions was observed only for the line carrying chromosome 4 which was more resistant than the susceptible control. The potential effects of two other chromosomes have to be confirmed. F1 hybrids obtained by crosses between two highly susceptible lines and the monosomic addition line carrying chromosome 4 were examined under field conditions. No effect of the oilseed rape genetic background on the expression of resistance was detected. The introduction of this resistance and mapping of the gene(s) into oilseed rape varieties are discussed.  相似文献   

Brassilexin accumulation and resistance to Leptosphaeria maculans in Brassica spp. and progeny of an interspecific cross B. juncea × B. napus     

Thierry Rouxel  Michel Renard  Albert Kollmann  Jean-Francois Bousquet 《Euphytica》1990,46(2):175-181

Summary Resistance to Leptosphaeria maculans was assessed in Brassica napus, B. juncea, B. carinata, B. nigra and progeny issuing from an interspecific cross B. napus × B. juncea, using a cotyledon-inoculation test. In these individual plants, brassilexin accumulation was determined following an abiotic, non-specific, elicitation. All the tested B. napus cultivars were highly susceptible to the parasite and weakly accumulated brassilexin. In contrast, B. juncea, B. carinata, and B. nigra usually displayed a hypersensitive response to the inoculation and accumulated more brassilexin than B. napus. The same correlation between resistance to L. maculans and phytoalexin accumulation was observed in the interspecific hybrid progeny. The cotyledon-inoculation test allowed the discrimination of plants displaying a hypersensitive response to the inoculation from those highly sensitive to the parasite, but intermediate disease severity classes were not usually representative of resistance or susceptibility. In this respect, brassilexin determination allowed differentiation, within a set of plants presenting an intermediate response to the pathogen, of plants with a high (B. juncea-like), and with a weak (B. napus-like) ability to accumulate brassilexin.Abbreviations IHP interspecific hybrid progeny - JR B. juncea-type complete resistance to blackleg (Roy, 1984) - W&D test cotyledon-inoculation test as described by Williams & Delwiche (1979)  相似文献   

Biology of the gametes of some teleost species     

R. Billard  R. Christen  M. P. Cosson  J. L. Gatty  L. Letellier  P. Renard  A. Saad 《Fish physiology and biochemistry》1986,2(1-4):115-120

Our knowledge of the biology of fish gametes is still limited. Up to now research was short-term, aiming at solving practical questions connected with gamete storage and artificial insemination. More information on gamete biology is now available and has been summarized in this minireview.In vivo storage of gametes in the genital tract after ovulation or spermiation may be influenced by the osmotic environmentvia changes in the ionic composition of the companion fluid. There is great inter-individual variability in gamete survival bothin vivo andin vitro. Mechanisms involved in the initiation of trout sperm motility have been analysed; after dilution in water or NaCl solution (125 mM) at pH > 7, Ca⁺⁺ entry due to changes in membrane potential have been observed. The involvement of cAMP and protein phosphorylation has been reported. The morphological, physical and biochemical changes in eggs that have been studied after fertilization and the corticol reaction lead to a better understanding of the mechanisms of polyspermy blockage.  相似文献