Influence of the apetalous character in rape (Brassica napus) on the foraging behaviour of honeybees (Apis mellifera)     

J. Pierre    J. S. Pierre    R. Marilleau    M. H. Pham-Delègue  X. Tanguy  M. Renard 《Plant Breeding》1996,115(6):484-487

In order to establish whether commercial hybrid seed production between apetalous and apetalous rapeseed lines could be developed through insect cross-pollination, observations were made to compare the foraging behaviour of honeybees on both flower types with regard to their attractiveness. Number of flowers/m² and nectar volumes, considered as important attractive factors, did not differ between the two lines but nectar sugar composition was slightly different. Honeybee density was significantly higher on the apetalous line when their foraging activity was rather high. Honeybees showed a considerable constancy to each flower type. Passages between both types were not very frequent. Nectar foraging behaviour, observed under cages, differed with the floral morphology. On the apetalous flowers, honeybees visited the two inner nectaries by crawling over the anthers and the stigma, while on the apetalous ones they often inserted their tongues between the sepals, thus having less contact with the reproductive organs. From these results, the honeybee efficiency in cross-pollination between petalous and apetalous lines appears to be reduced.  相似文献   

Use of hair root as a source of DNA for the detection of heterozygotes for recessive defects in cattle     

PJ HEALY  JA DENNIS  JF MOULE 《Australian veterinary journal》1995,72(10):392-392

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Molecular cloning of the mRNA coding for the G protein of the viral haemorrhagic septicaemia (VHS) of salmonids   总被引：1，自引：0，他引：1  

M Thiry  F Lecocq-Xhonneux  I Dheur  A Renard  P de Kinkelin 《Veterinary microbiology》1990,23(1-4):221-226

Viral haemorrhagic septicaemia virus (VHSV), a rhabdovirus, is a major threat for continental European trout fish farming. The development of a recombinant subunit vaccine could solve that problem. The neutralizing epitopes are located on the glycoprotein or G protein, the surface antigen. The G protein has a molecular weight of 65 kDa, reduced to 55 kDa by deglycosylation. cDNA was synthetized from mRNA of VHS virus infected cells, and cloned in E. coli. The viral cDNA was recognized by positive hybridization with a labelled probe made from infected cell RNA, and negative hybridization with labelled cDNA made from cellular RNA. The Northern blot hybridization with different clones on VHS infected cell RNA revealed two VHS mRNA whose lengths, 2.0 and 1.5 kb, were compatible with the mRNA length for G and N proteins respectively. This mRNA must contain about 400 bp of untranslated sequence.  相似文献   

Retrospective evaluation of the efficacy of isolating bacteria from synovial fluid in dogs with suspected septic arthritis          下载免费PDF全文

VF Scharf  ST Lewis  JF Wellehan  HL Wamsley  R Richardson  DA Sundstrom  DD Lewis 《Australian veterinary journal》2015,93(6):200-203

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Colour Doppler Ultrasonography as a Tool to Assess Luteal Function in Santa Inês Ewes          下载免费PDF全文

LM Figueira  JF Fonseca  EKN Arashiro  JMG Souza‐Fabjan  ACS Ribeiro  E Oba  JHM Viana  FZ Brandão 《Reproduction in domestic animals》2015,50(4):643-650

The aim of this study was to evaluate luteal dynamics in the Santa Inês ewes using colour Doppler (CD) ultrasonography. Oestrus was synchronized in nulliparous females (n = 18), and subsequently, they were only teased (n = 6) or teased and mated (n = 12). Blood samples were collected daily for plasma progesterone (P₄) concentrations. Ultrasonographic images of corpora lutea (CL) in CD mode were obtained for further analysis in its largest diameter. The CD mode allowed an early sequential monitoring of CL that was visualized by the first time 0.77 ± 0.62 days after ovulation, with luteal area 29.68 ± 13.21 mm². During the luteogenesis, a progressive increase was observed, followed by a plateau of luteal area, vascularization area and plasma concentrations of P₄ reaching maximum values in D11 (124.0 ± 38.0 mm², 52.78 ± 24.08 mm² and 11.23 ± 4.89 ng/ml, respectively). In the luteolysis, the plasma concentrations of P₄ decreased sharply, whereas luteal and vascularization area gradually. The vascularization area was positively correlated with plasma concentrations of P₄ during the luteogenesis (r = 0.22) and luteolysis (r = 0.48). The luteal dynamics of Santa Inês ewes showed patterns similar to those observed in other sheep breeds studied. The CD ultrasonography has the potential to be used as a tool to assess luteal function in sheep.  相似文献   

Nucleotide sequence and expression of an AIDS-associated retrovirus (ARV-2)   总被引：139，自引：0，他引：139  

R Sanchez-Pescador  M D Power  P J Barr  K S Steimer  M M Stempien  S L Brown-Shimer  W W Gee  A Renard  A Randolph  J A Levy 《Science (New York, N.Y.)》1985,227(4686):484-492

The nucleotide sequence of molecular clones of DNA from a retrovirus, ARV-2, associated with the acquired immune deficiency syndrome (AIDS) was determined. Proviral DNA of ARV-2 (9737 base pairs) has long terminal repeat structures (636 base pairs) and long open reading frames encoding gag (506 codons), pol (1003 codons), and env (863 codons) genes. Two additional open reading frames were identified. Significant amino acid homology with several other retroviruses was noted in the predicted product of gag and pol, but ARV-2 was as closely related to murine and avian retroviruses as it was to human T-cell leukemia viruses (HTLV-I and HTLV-II). By means of an SV-40 vector in transfected simian cells, the cloned gag and env genes of ARV-2 were shown to express viral proteins.  相似文献   

Lectin Histochemistry as a Tool to Identify Apoptotic Cells in the Seminiferous Epithelium of Syrian Hamster (Mesocricetus auratus) Subjected to Short Photoperiod     

V Seco‐Rovira  E Beltrán‐Frutos  C Ferrer  MM Sánchez‐Huertas  JF Madrid  FJ Saez  LM Pastor 《Reproduction in domestic animals》2013,48(6):974-983

Lectins have been widely used to study the pattern of cellular glycoconjugates in numerous species. In the process of cellular apoptosis, it has been observed that changes occur in the membrane sugar sequences of these apoptotic cells. The aim of our work was to identify which lectins, out of an extensive battery of the same (PNA, SBA, HPA, LTA, Con‐A, UEA‐I, WGA, DBA, MAA, GNA, AAA, SNA), show affinity for germinal cells in apoptosis, at what stage of cell death they do so and in which germinal cell types they can be detected. For this, we studied testis sections during testicular regression in Syrian hamster (Mesocricetus auratus) subjected to short photoperiod. Several lectins showed an affinity for the glycoconjugate residues of germ cells in apoptosis: Gal β1,3‐GalNAcα1, α‐d ‐mannose, N‐acetylgalactosamine and l ‐fucose. Furthermore, lectin specificity was observed for some specific germinal cells and in certain stages of apoptosis. It was also observed that one of these lectins (PNA) showed affinity for Sertoli cells undergoing apoptosis. Therefore, we conclude that the use of lectin histochemistry could be a very useful tool for studying apoptosis in the seminiferous epithelium because of the specificity shown towards germinal cells in pathological or experimentally induced epithelial depletion models.  相似文献   

Necrotising fasciitis in a bull due to infection with Arcanobacterium haemolyticum     

JB Bancroft-Hunt  JF Weston  JS Munday 《New Zealand veterinary journal》2013,61(5):260-264

Abstract

CASE HISTORY: A 2-year-old Hereford bull was lame for one week before becoming recumbent.

CLINICAL AND PATHOLOGICAL FINDINGS: The scrotum and ventral perineal region were cold and blackened caudally. The semimembranosus and semitendinosus muscles were firm on palpation. The bull was subject to euthanasia, and necropsy revealed that the skin and S/C tissues of the caudal half of the scrotum were grey and necrotic. The caudal and distal aspects of the semimembranosus and semitendinosus muscles were grey and necrotic to a depth of approximately 15 cm, and these changes appeared to track along fascial planes. The tissue had an offensive smell, and large amounts of flocculent, watery, brown fluid and some gas were present. Histology of affected muscle and S/C tissues revealed coagulative necrosis, with oedema and large numbers of bacteria that were predominantly Gram-positive rods. Adjacent blood vessels contained thrombi while the epidermis overlying the affected areas appeared diffusely necrotic, suggesting infarction. Culture of the fluid yielded a pure growth of Arcanobacterium spp., which was identified as Arcanobacterium haemolyticum, using an API Coryne biochemical test strip.

DIAGNOSIS: Necrotising fasciitis and myositis due to Arcanobacterium haemolyticum.

CLINICAL RELEVANCE: Arcanobacterium haemolyticum has not previously been reported as a cause of necrotising fasciitis in any species. Necrotising fasciitis is probably an under-reported condition in cattle due to its clinical similarity to clostridial disease.  相似文献   

Genetic control of yield and yield components in winter oilseed rape (Brassica napus L.) grown under nitrogen limitation   总被引：1，自引：0，他引：1  

Anne-Sophie Bouchet  Nathalie Nesi  Christine Bissuel  Michel Bregeon  Amandine Lariepe  Hélène Navier  Nicolas Ribière  Mathilde Orsel  Bruno Grezes-Besset  Michel Renard  Anne Laperche 《Euphytica》2014,199(1-2):183-205

Despite its high nitrogen absorption capacity, oilseed rape (OSR) has a low apparent nitrogen use efficiency (NUE), which makes its production highly dependent on nitrogen fertilization. Improving NUE in OSR is therefore a main target in breeding. The objectives of the present work were to determine the genomic regions (QTLs) associated with yield and to assess their stability under contrasted nitrogen nutrition regimes. One mapping population, AM, was tested in a French location for three growing seasons (2011, 2012 and 2013), under two nitrogen conditions (optimal and low). Eight yield-related traits were scored and nitrogen-responsive traits were calculated. A total of 104 QTLs were detected of which 28 controlled flowering time and 76 were related to yield and yield components. Very few genotype × nitrogen interactions were detected and the QTLs were highly stable between the nitrogen conditions. In contrast, only a few QTLs were stable across the years of the trial, suggesting a strong QTL × year interaction. Finally, eleven critical genomic regions that were stable across nitrogen conditions and/or trial years were identified. One particular region located on the A5 linkage group appears to be a promising candidate for marker assisted selection programs. The different strategies for OSR breeding using the QTLs found in the present study are discussed.  相似文献   

Veterinary dairy herd fertility service provision in seasonal and non-seasonal dairy industries - a comparison     

Mee J 《Irish veterinary journal》2010,63(4):230-235

The decline in dairy herd fertility internationally has highlighted the limited impact of traditional veterinary approaches to bovine fertility management. Three questionnaire surveys were conducted at buiatrics conferences attended by veterinary practitioners on veterinary dairy herd fertility services (HFS) in countries with a seasonal (Ireland, 47 respondents) and non-seasonal breeding model (The Netherlands, 44 respondents and Portugal, 31 respondents). Of the 122 respondents, 73 (60%) provided a HFS and 49 (40%) did not. The majority (76%) of all practitioners who responded stated that bovine fertility had declined in their practice clients' herds with inadequate cow management, inadequate nutrition and increased milk yield as the most important putative causes. The type of clients who adopted a herd fertility service were deemed more educated than average (70% of respondents), and/or had fertility problems (58%) and/or large herds (53%). The main components of this service were routine postpartum examinations (95% of respondents), fertility records analysis (75%) and ultrasound pregnancy examinations (69%). The number of planned visits per annum varied between an average of four in Ireland, where breeding is seasonal, and 23 in Portugal, where breeding is year-round. The benefits to both the practitioner and their clients from running a HFS were cited as better fertility, financial rewards and job satisfaction. For practitioners who did not run a HFS the main reasons given were no client demand (55%) and lack of fertility records (33%). Better economic evidence to convince clients of the cost-benefit of such a service was seen as a major constraint to adoption of this service by 67% of practitioners.  相似文献