Maturation Status, Protein Synthesis and Developmental Competence of Oocytes Derived from Lambs and Ewes   总被引：2，自引：0，他引：2  

HPS Kochhar  B Wu  LHA Morris  BC Buckrell  JW Pollard  PK Basrur  & WA King 《Reproduction in domestic animals》2002,37(1):19-25

The efficacy of oocyte selection for in vitro embryo production depends on the abundance and diameter of follicles, cumulus layers around the oocytes and subsequent fertilization. Application of `ovum pick-up' technique allows us to utilize partially matured oocytes for embryo production even from juvenile subjects. To compare their developmental competence, oocytes derived from lambs and ewes and cultured in maturation medium for up to 26 h were assessed at 2 h intervals by confocal microscopy after chromatin and microtubulin-specific fluorochrome labelling. Lamb oocytes reached second meiotic metaphase (MII) at lower numbers at 24 h (60.0%) and 26 h (28.6%) whereas 85.7% of adult-derived oocytes attained MII status by 24 h of maturation. Radiolabelling of oocyte proteins revealed higher incorporation of [³⁵S-]-methionine and [³⁵S]-cysteine in adult-derived oocytes compared to lamb oocytes. Although the cleavage rate of lamb oocytes was similar to that of ewe oocytes, the proportion reaching blastocyst stage was significantly lower (p < 0.05) in the lamb-derived oocytes. However, blastocysts from both types of oocytes displayed similar cell lineage allocations to inner cell mass and trophectoderm.  相似文献   

Blindness in South Australian Kangaroos     

PJK DURHAM  JW FINNIE  DA LAWRENCE  P. ALEXANDER 《Australian veterinary journal》1996,73(3):111-111

  相似文献   

The efficacy of a controlled-release albendazole capsule in suppressing nematode burdens in sheep   总被引：3，自引：0，他引：3  

NJ BARTON  B. RODDEN†  JW STEEL‡ 《Australian veterinary journal》1990,67(11):408-410

An experiment was undertaken between July and November 1985 in East Gippsland, Victoria, to determine the efficacy of an intra-ruminal controlled-release albendazole capsule against naturally acquired worm burdens and larval challenge in Merino hoggets. Two groups of 20 sheep, one group untreated, the other dosed with a capsule were grazed together; 5 sheep from each group were slaughtered for total worm counts 30 and 101 d after capsules were administered. Serum anthelmintic concentrations, faecal egg counts and body weights were monitored. Most capsules were exhausted within 91 d of administration. During the estimated 80 d for which they remained active the capsules were highly effective against the benzimidazole-susceptible worm populations. Faecal egg counts were reduced to zero and total worm populations were reduced by over 97% 30 d after administration. By 101 d egg counts were increasing and worm counts indicated that sheep were becoming reinfected. Sheep treated with the capsules grew faster than those not treated.  相似文献   

A collapse syndrome associated with Gram-negative infection in cows treated with dexamethasone to induce parturition     

JW BROWNING  KJ SLEE  J. MALMO  P. BRIGHTLING 《Australian veterinary journal》1990,67(1):28-29

  相似文献   

Progressive ataxia associated with degenerative thoracic myelopathy in Merino sheep     

PAW HARPER †  JW PLANT  KH WALKER  KG TIMMINS† 《Australian veterinary journal》1991,68(11):357-358

A neurological disorder in Merino sheep, characterised clinically by progressive posterior ataxia and microscopically by Wallerian degeneration in thoracic segments of the spinal cord, is described. Animals of both sexes were affected, with the earliest onset of disease being at 5 months of age. Most affected animals died before 2 years of age. The clinical, pathological and epidemiological features suggest that this degenerative thoracic myelopathy is a previously unrecognised entity differing from other reported causes of ataxia in sheep in Australia.  相似文献   

Characterization of mRNA Expression in Preimplantation Bovine Embryos     

C Wrenzycki  D Herrmann  E Lemme  K Korsawe  JW Carnwath  H Niemann 《Reproduction in domestic animals》1998,33(3-4):307-311

  相似文献   

Detection of Toxoplasma gondii DNA in Fresh and Frozen Semen from Rams in Brazil     

MJG Bezerra  JALO Cruz  ES Kung  PPF Albuquerque  PCP Kim  EPBX Moraes  JW Pinheiro Júnior  RA Mota 《Reproduction in domestic animals》2014,49(5):753-755

The aim of this study was to estimate the prevalence of genomic DNA of Toxoplasma gondii in semen samples from commercial rams in artificial insemination centres in Brazil, as well as in fresh semen from rams in the northeast of Brazil. In total, 108 semen samples were obtained from artificial insemination centres, and genomic DNA of T. gondii was detected in 24 of 108 (22.2%). The prevalence of antibodies anti‐Toxoplasma gondii among sheep on rural properties was 9.2% (10/109), and 100% of the semen samples of these animals were positive in the PCR for T. gondii DNA. The molecular identity was confirmed through sequencing, which indicated 99.9% similarity with the T. gondii DNA sequences stored in the GenBank. This study reports the first occurrence of T. gondii DNA in the semen of rams, which came from artificial insemination centres in Brazil, as well as the occurrence of T. gondii DNA in the fresh semen of naturally infected rams in the northeast of Brazil.  相似文献   

Traumatic head injury in ruminant livestock     

JW FINNIE 《Australian veterinary journal》1997,75(3):204-208

  相似文献   

Effect of phosphate rock, lime and cellulose on soil microbial biomass in acidic forest soil and its significance in carbon cycling     

Z. L. He  V. C. Baligar  D. C. Martens  K. D. Ritchey 《Biology and Fertility of Soils》1997,24(3):329-334

Phosphate rock (PR), limestone, coal combustion by-product (CCBP) high in Ca and high organic manures are potential amendments for increasing agricultural production in the acidic soils of the Appalachian region. The objective of this study was to examine effects of PR, CCBP and cellulose addition on soil microbial biomass in an acidic soil based on the measurement of soil microbial biomass P (P _mic) and on the mineralization of organic matter. Application of PR alone or in combination with CCBP increased P _mic. The P _mic was far less when the soil received PR in combination with limestone than with PR application alone or PR in combination with CCBP. Either CCBP or limestone application alone considerably decreased P _mic in the soil due to reduced P solubility. Cellulose addition alone did not increase P _mic, but P _mic was significantly increased when the soil was amended with cellulose in combination with PR. The decomposition of added cellulose was very slow in the soil without PR amendment. However, mineralization of both native organic matter and added cellulose was enhanced by PR application. Mineralization of organic matter was less when the soil was amended with PR in combination with high rates of CCBP (> 2.5%) because PR dissolution varied inversely with amount of CCBP addition. Overall, CCBP had no detrimental effect on soil microbial biomass at low application rates, although, like limestone, CCBP at a high rate may decrease P _mic in P-deficient soils through its influence on increased soil pH and decreased P bioavailability in the soil. Application of PR to an acidic soil considerably enhanced the microbial activity, thereby promoting the cycling of carbon and other nutrients. Received: 11 December 1995  相似文献   

Use of ultrasound‐guided autologous bone marrow transfer for treatment of suspensory ligament desmitis in 30 race horses (2003–2010)     

MS Hall  JR Vasey  JW Russell  T Russell 《Australian veterinary journal》2013,91(3):102-107

  相似文献