The Influence of Oxygen Tension on Cumulus Cell Viability of Canine COCs Matured in High-Glucose Medium     

AEF Silva  P Rodriguez  LF Cavalcante  BA Rodrigues  JL Rodrigues 《Reproduction in domestic animals》2009,44(S2):259-262

High in vitro oxygen (O₂) tensions are associated with enhanced levels of reactive oxygen species and cumulus oocyte complex (COC) apoptosis. The objective of this study was to determine the influence of O₂ tension on cumulus cell (CC) viability from canine oocytes. Cumulus oocyte complexes were distributed into three groups (CG, T20 and T5) and two O₂ tension levels (20% and 5%). The control group (CG) was matured in vitro in a humidified atmosphere with 5% CO₂ in air in TCM199 with 26.19 m m sodium bicarbonate, 10% (v/v) foetal calf serum (FCS), 0.10 m m gentamicin, 0.20 m m pyruvic acid, 20 μg/ml oestradiol, 0.5 μg/ml follicle-stimulating hormone, 0.03 IU/ml human chorionic gonadotropin, and 1.0 μg/ml human somatotropin. Groups T20 and T5 were matured under 20% or 5% O₂ tensions respectively in a high-glucose medium, without FCS. T20 and T5 were as CG, and supplemented with 0.1% Polyvinyl Alcohol, and 5.5 m m glucose. After 48 h of IVM, CCs from COCs were stained with propidium iodide (1.50 m m ). The results showed that viability of CCs (cytoplasmic features and nuclear morphological integrity) was different for the three groups. Rates of apoptosis were at 57.9% (521/900) for CG, 54.4% (490/900) for T20 and 38.9% (350/900) for T5 (p < 0.001). Predominant features in apoptotic cells (n = 1361) were DNA nuclear fragments (94.0%). It was concluded that CCs of canine COCs cultured in high-glucose medium showed significantly less apoptosis than those cultured in medium with FCS. Low O₂ tension was efficient in reducing apoptosis in canine CCs.  相似文献   

Seizures during medetomindine sedation and local anaesthesia in two dogs undergoing skin biopsy   总被引：1，自引：1，他引：0  

JE Rainger  CG Baxter  LJ Vogelnest  CM Dart 《Australian veterinary journal》2009,87(5):188-192

Each of two dogs presented for multiple skin biopsies were sedated with intravenous medetomidine and lignocaine was injected subcutaneously to provide local anaesthesia for skin biopsy. One dog had a seizure during skin biopsy and again immediately following reversal of medetomidine with atipamezole. The other dog developed seizures 2 h following skin biopsy at which time the medetomidine was reversed with atipamezole. Both dogs were neurologically normal with no history of seizures prior to the procedure and remained neurologically normal for 14 weeks and 9 months, respectively, following the procedure. A drug interaction between the α₂-adrenergic agonist medetomidine and lignocaine is suspected and highlights the potential for seizures following the subcutaneous administration of relatively large doses of lignocaine under medetomidine sedation.  相似文献   

Uterine adenomyosis in an orang-utan (Pongo abelii/pygmaeus)     

KJ Graham  FA Hulst  L Vogelnest  IS Fraser  CM Shilton 《Australian veterinary journal》2009,87(1-2):66-69

A 48-year-old, multiparous, female hybrid orang-utan ( Pongo abelii/pygmaeus ) was investigated after a 3-year history of irregular and excessively heavy menstrual bleeding. Opportunistic pelvic examinations over a 2.5-year period were non-diagnostic. Medical therapy was not effective. A subtotal hysterectomy with bilateral salpingo-ovariectomy was performed. A pedunculated mass spanning 90% of the uterine lumen was seen grossly, and histopathology confirmed uterine adenomyosis. Adenomyosis is defined as the ectopic occurrence or diffuse implantation of endometrial tissue, including glands and stroma, into the myometrium. It is common in older, usually premenopausal, multiparous women and is frequently associated with other uterine pathology, including endometrial hyperplasia and leiomyomas. The most common clinical signs are dysmenorrhoea and heavy menstrual bleeding; however, up to 35% of women are asymptomatic. Diagnosis is difficult and requires myometrial sampling and an experienced pathologist. A hysterectomy in this case was diagnostic and curative. There have been few reports of uterine adenomyosis in non-human primates and none reported in an orang-utan. Uterine adenomyosis should be considered in the differential diagnosis in any multiparous, aged, non-human female primate with irregular and excessively heavy menstrual bleeding, and hysterectomy with bilateral salpingo-ovariectomy is recommended as a diagnostic and therapeutic solution.  相似文献   

Factors associated with gastrointestinal parasite infection in dogs in Rio de Janeiro, Brazil     

Bianca Chiganer Cramer Balassiano  Mnica Rodrigues Campos  Rita de Cssia Alves Alcantara de Menezes  Maria Julia Salim Pereira 《Preventive veterinary medicine》2009,91(2-4):234-240

Factors associated with parasitism by helminths and protozoans in 500 dogs presented to three veterinary clinics in the municipality of Rio de Janeiro from November 2003 to September 2004 were evaluated. Dogs were submitted to physical examination and owners were interviewed about the animal's management. One fecal sample from each dog was examined by centrifugal flotation and sedimentation methods followed by the safranin–methylene blue staining technique. Positive results for gastrointestinal parasites were detected in 46.4% of the examined samples. Infection with protozoans (29.6%) was more frequent than with helminths (23.2%). Cryptosporidium sp. (26.2%) and Ancylostoma sp. (15.2%) were the most frequent parasites. Logistic regression analysis showed that age (p < 0.001), access to soil (p < 0.001), hygiene of the environment (p = 0.001), illness (p = 0.007), owner's level of education (p < 0.006) and veterinary clinic (p = 0.043) were associated with gastrointestinal parasite infections in dogs. Treatment and control are especially important for puppies. Adult dogs should be submitted to fecal examination before treatment, placing special emphasis on those that present one or more factors associated with infection.  相似文献   

Intralesional amphotericin B in a cat with refractory localised sporotrichosis     

Isabella D.F. Gremião  Tânia Maria P. Schubach  Sandro A. Pereira  Aline M. Rodrigues  Adriana R. Chaves  Mônica B.L. Barros 《Journal of Feline Medicine and Surgery》2009,11(8):720-723

  相似文献   

Net requirements of calcium, magnesium, sodium, phosphorus, and potassium for growth of Nellore × Red Angus bulls, steers, and heifers   总被引：1，自引：0，他引：1  

Mario Luiz Chizzotti  Sebastio de Campos Valadares Filho  Luis Orlindo Tedeschi  Pedro Veiga Rodrigues Paulino  Mrio Fonseca Paulino  Rilene Ferreira Diniz Valadares  Paloma Amaral  Pedro Del Bianco Benedeti  Tainnah Ikegami Rodrigues  Mozart Alves Fonseca 《Livestock Science》2009,124(1-3):242-247

A comparative slaughter trial was conducted to assess the net requirements for gain of Ca, P, Na, K and Mg of bulls, steers and heifers of Nellore and Red Angus crossbreds. Twenty seven F1 Nellore and Red Angus crossbred calves (9 steers, 9 bulls, and 9 heifers), averaging 274 kg BW, were used. At the beginning of the trial, three animals from each gender were slaughtered to determine the initial body composition. The remaining 18 animals (3 animals of each gender) were randomly assigned to two treatments: fed 0.75 or 1.5% of BW of concentrate. The diets were based on corn silage and were isonitrogenous (2% N, DM). After three growing periods of 28 d, all animals were slaughtered. The cleaned gastrointestinal tract, organs, carcass, head, hide, tail, feet, and tissues were weighed to determine the empty BW (EBW). These parts were ground separately and subsampled for chemical analyses. The log of the contents of each mineral in the empty body was regressed on the log of the EBW to estimate the net requirement for each mineral per kg of empty body gain (EBG). There were no differences (P > 0.05) in the net requirements for growth of all macrominerals among genders. The equations of the pooled data of the net requirements for growth (g/kg EBG) were: 332.6 × EBW ^− 0.6367 for Ca, 112.1 × EBW ^− 0.5615 for P, 10.85 × EBW ^− 0.3992 for Na, 4.01 × EBW ^− 0.153 for K, and 3.589 × EBW ^− 0.462 for Mg. Our findings indicated that retained Ca and retained P were poorly related to the retained protein.  相似文献   

Physicochemical and Functional Properties of Protein Recovered from Fish Waste     

Irene Rodrigues Freitas  William Renzo Cortez-Vega  Carlos Prentice 《Journal Of Aquatic Food Product Technology》2013,22(7):1034-1044

ABSTRACT

This work aimed to evaluate the physicochemical and functional properties of proteins recovered from whitemouth croaker (Micropogonias furnieri) and Argentine anchovy (Engraulis anchoita) residues obtained by the process of acid solubilization using HCl at pH = 2 and alkaline solubilization using NaOH at pH = 12 and isoelectric precipitation (pH = 5.5). The proximate composition and functional properties were determined. The solubilization and water holding capacity (WHC) of the recovered protein were studied in the pH range of 3, 5, 7, 9, and 11. The high protein content and lowest lipid reduction presented by the protein recovered from the whitemouth croaker residue and obtained by the acid solubilizing process was 97.1 and 98.4%, respectively. The maximum solubilized protein was at pH 3 and 11, and the minimum solubilized protein was at pH 5 for both species. The greater ability to retain oil was observed for the alkaline solubilization process (8.3 and 7.3). The lower WHC was at pH 5. The acid and alkali residue concentrates of the whitemouth croaker presented higher WHC at pH 3 and 11. These proteins can be used in preparing edible films and have been used to produce bioactive compounds for supplements or functional ingredients.  相似文献   

Release of doxycycline through cellulose acetate symmetric and asymmetric membranes produced from recycled agroindustrial residue: Sugarcane bagasse     

Guimes Rodrigues Filho  Sabrina Dias RibeiroCarla da Silva Meireles  Leandro Gustavo da SilvaReinaldo Ruggiero  Moacir Fernandes Ferreira JuniorDaniel Alves Cerqueira  Rosana Maria Nascimento de AssunçãoMara Zeni  Patricia Polleto 《Industrial Crops and Products》2011,33(3):566-571

Cellulose acetate is one of the components employed in drug controlled-release systems in the form of membranes. The aim of this study was to examine the controlled-release of doxycycline employing cellulose acetate symmetric and asymmetric membranes as matrices. The cellulose triacetate was produced from sugarcane bagasse through a homogeneous acetylation reaction, using acetic acid as the solvent, acetic anhydride as the acetylating agent and sulfuric acid as the catalyst. The viscosity average molecular weight of the cellulose acetate produced was 39,000 g mol⁻¹. The symmetric membranes were produced using a system solvent of dichloromethane/ethanol (9:1, v/v) and the asymmetric membranes were produced from the same solvent system and 10% of water. For the formulation of both, 5% of doxycycline was used. The membranes were characterized by thermal analysis (DSC and TGA) and scanning electron microscopy SEM. The release of doxycycline through cellulose triacetate matrices was examined using spectrophotometric analysis in the ultraviolet-visible region, at 275 nm. The results revealed that asymmetric membranes release 80% of the drug in 100 min, while symmetric membranes release 14% of the drug during the same time interval.  相似文献   

Effects of pneumoperitoneum and of an alveolar recruitment maneuver followed by positive end-expiratory pressure on cardiopulmonary function in sheep anesthetized with isoflurane–fentanyl     

Jéssica C. Rodrigues  Francisco J. Teixeira-Neto  Sofia A. Cerejo  Nathalia Celeita-Rodríguez  Natache A. Garofalo  Juliany G. Quitzan  Thalita L.A. Rocha 《Veterinary anaesthesia and analgesia》2017,44(4):841-853

Objective

To investigate the effects of pneumoperitoneum alone or combined with an alveolar recruitment maneuver (ARM) followed by positive end-expiratory pressure (PEEP) on cardiopulmonary function in sheep.

Study design

Prospective, randomized, crossover study.

Animals

A total of nine adult sheep (36–52 kg).

Methods

Sheep were administered three treatments (≥10-day intervals) during isoflurane–fentanyl anesthesia and volume-controlled ventilation (tidal volume: 12 mL kg^?1) with oxygen: CONTROL (no intervention); PNEUMO (120 minutes of CO₂ pneumoperitoneum); PNEUMO_ARM/PEEP (PNEUMO protocol with an ARM instituted after 60 minutes of pneumoperitoneum). The ARM (5 cmH₂O increases in PEEP of 1 minute duration until 20 cmH₂O of PEEP) was followed by 10 cmH₂O of PEEP until the end of anesthesia. Cardiopulmonary data were recorded until 30 minutes after abdominal deflation.

Results

PaO₂ was decreased from 435–462 mmHg (58.0–61.6 kPa) (range of mean values in CONTROL) to 377–397 mmHg (50.3–52.9 kPa) in PNEUMO (p < 0.05). Quasistatic compliance (C_qst, mL cmH₂O^?1 kg^?1) was decreased from 0.85–0.92 in CONTROL to 0.52–0.58 in PNEUMO. PaO₂ increased from 383–385 mmHg (51.1–51.3 kPa) in PNEUMO to 429–444 mmHg (57.2–59.2 kPa) in PNEUMO_ARM/PEEP (p < 0.05) and C_qst increased from 0.52–0.53 in PNEUMO to 0.70–0.74 in PNEUMO_ARM/PEEP. Abdominal deflation in PNEUMO did not restore PaO₂ and C_qst to control values. Cardiac index (L minute^?1 m²) decreased from 4.80–4.70 in CONTROL to 3.45–3.74 in PNEUMO and 3.63–3.76 in PNEUMO_ARM/PEEP. Compared with controls, ARM/PEEP with pneumoperitoneum decreased mean arterial pressure from 81 to 68 mmHg and increased mean pulmonary artery pressure from 10 to 16 mmHg.

Conclusions and clinical relevance

Abdominal deflation did not reverse the pulmonary function impairment associated with pneumoperitoneum. The ARM/PEEP improved respiratory compliance and reversed the oxygenation impairment induced by pneumoperitoneum with acceptable hemodynamic changes in healthy sheep.  相似文献   

Immortalization and characterization of a new canine mammary tumour cell line FR37‐CMT          下载免费PDF全文

L. R. Raposo  C. Roma‐Rodrigues  P. Faísca  M. Alves  J. Henriques  M. C. Carvalheiro  M. L. Corvo  P. V. Baptista  A. J. Pombeiro  A. R. Fernandes 《Veterinary and comparative oncology》2017,15(3):952-967

Here we describe the establishment of a new canine mammary tumour (CMT) cell line, FR37‐CMT that does not show dependence on female hormonal signaling to induce tumour xenografts in NOD‐SCID mice. FR37‐CMT cell line has a stellate or fusiform shape, displays the ability to reorganize the collagen matrix, expresses vimentin, CD44 and shows the loss of E‐cadherin which is considered a fundamental event in epithelial to mesenchymal transition (EMT). The up‐regulation of ZEB1, the detection of phosphorylated ERK1/2 and the downregulation of DICER1 and miR‐200c are also in accordance with the mesenchymal characteristics of FR37‐CMT cell line. FR37‐CMT shows a higher resistance to cisplatin (IC₅₀>50 µM) and to doxorubicin (IC₅₀>5.3 µM) compared with other CMT cell lines. These results support the use of FR37‐CMT as a new CMT model that may assist the understanding of the molecular mechanisms underlying EMT, CMT drug resistance, fostering the development of novel therapies targeting CMT.  相似文献