Growth factor and cytokine interactions in myogenesis. Part II. Expression of IGF binding proteins and protein kinases essential for myogenesis in mouse C2C12 myogenic cells exposed to TNF-alpha and IFN-gamma     

Wieteska-Skrzeczyńska W  Grzelkowska-Kowalczyk K  Rejmak E 《Polish journal of veterinary sciences》2011,14(3):425-431

The aim of the study was to examine potential interactions among IGF-I and proinflammatory cytokines, TNF-alpha and IFN-gamma, in the regulation of local IGF-I bioavailability and cellular proteins mediating myogenic signals. We investigated levels of IGFBP-4, -5, -6, protein kinase Czeta (PKC zeta), p38 and extracellular signal-regulated kinase (ERK1/2) in differentiating mouse C2C12 myoblasts. IGF-I significantly stimulated expression of IGFBP-5. TNF-alpha and IFN-gamma attenuated the expression of IGFBP-4 and -6 under basal conditions and in the presence of IGF-I, and inhibited IGF-I-induced IGFBP-5 expression during 5-day myogenesis. TNF-alpha and IFN-gamma markedly attenuated p38 expression in the presence of IGF-I on the 5th day of myogenesis. When combined with IGF-I the cytokines exerted opposite effects on the PKC zeta level, i.e. TNF-alpha caused an increase, whereas IFN-gamma reduced the cellular content of this kinase. Exposition of C2C12 myoblasts to IGF-I or cytokines led to the stimulation of ERK1/2 phosphorylation; however, both TNF-alpha and IFN-gamma exerted an inhibitory effect on the activation of ERK1/2 in myoblasts cultured in the presence of IGF-I. We concluded as follows: i) TNF-alpha and IFN-gamma present in the extracellular environment of differentiating C2C12 myoblasts can alter the local bioavailability of IGF-I by inhibiting the expression of IGFBP-4, -5, and -6, ii) the decrease in p38 expression and ERK1/2 phosphorylation in C2C12 myoblasts exposed to cytokines can lead to disturbances in IGF-I-regulated myogenesis.  相似文献   

The effects of prenatal exposure to methylxanthines     

Skopiński P  Woronkowicz M  Skopińska-Rózewska E  Siwicki AK 《Polish journal of veterinary sciences》2011,14(4):695-701

This review discusses epidemiology and laboratory studies on the effects of prenatal methylxanthine administration on some systems developing organisms. They are mainly absorbed from coffee, tea and cocoa products such as cola beverages and chocolate bars. Prenatal methylxanthine exposure can induce several unfavourables changes in the developing organism, which are persistent even in later phases of life. Based on results obtained from animal studies, the effect on embryogenesis is not only poorly understood but also controversial. It is therefore important to study interspecies differences as results may differ depending on animals used and administration methods.  相似文献   

The effect of diets supplemented with fish broth and fish oil on the health of weaners     

Bakuła T  Lis Ł  Iwaniuk Z  Ordyński Z 《Polish journal of veterinary sciences》2011,14(2):219-225

The aim of this study was to determine the effect of fish-based feed materials, as a source of readily available protein contained in fish broth and essential polyunsaturated fatty acids (PUFAs) found in fish oil, on the health of piglets and rearing results. The experiment was conducted on a commercial pig fattening farm. The study involved a total of 80 weaners with an approximate body weight of 15 kg. The experiment was carried out over a period of 40 days. Feed samples were subjected to laboratory analyses. Blood samples were collected from experimental group animals to determine serum biochemical and immunological parameters. The body weight gains of weaners, mortality rates and average feed intake per animal were calculated for the entire experimental period. The addition of fish broth and fish oil significantly improved the n3:n6 fatty acid ratio in diets. The presence of EPA and DHA in the experimental diet could have had a positive health effect on piglets, comparable with that exerted by therapeutic doses of zinc often administered to pigs of this age group. During the experiment, feed conversion ratio (FCR) gain was considerably reduced in the experimental group, with similar daily gains in the control and experimental group.  相似文献   

New natural host plants of ‘Candidatus Phytoplasma pini’ in Poland and the Czech Republic     

M. Kamińska  H. Berniak  J. Obdrzalek 《Plant pathology》2011,60(6):1023-1029

The presence of phytoplasmas in seven coniferous plant species (Abies procera, Pinus banksiana, P. mugo, P. nigra, P. sylvestris, P. tabuliformis and Tsuga canadensis) was demonstrated using nested PCR with the primer pairs P1/P7 followed by R16F2n/R16R2. The phytoplasmas were detected in pine trees with witches’ broom symptoms growing in natural forest ecosystems and also in plants propagated from witches’ brooms. Identification of phytoplasmas was done using restriction fragment length polymorphism analysis (RFLP) of the 16S rDNA gene fragment with AluI, MseI and RsaI endonucleases. All samples showed RFLP patterns similar to the theoretical pattern of ‘Candidatus Phytoplasma pini’, based on the sequence of the reference isolate Pin127S. Nested PCR‐amplified products, obtained with primers R16F2n/R16R2, were sequenced. Comparison of the 16S rDNAs obtained revealed high (99·8–100%) nucleotide sequence identity between the phytoplasma isolates. The isolates were also closely related to four other phytoplasma isolates found in pine trees previously. Based on the results of RFLP and sequence analyses, the phytoplasma isolates tested were classified as members of the ‘Candidatus Phytoplasma pini’, group 16SrXXI.  相似文献   

Dietary behaviour of German adults differing in levels of sport activity     

Beitz R  Mensink GB  Henschel Y  Fischer B  Erbersdobler HF 《Public health nutrition》2004,7(1):45-52

  相似文献   

Food security in the Baltic Republics     

Pomerleau J  McKee M  McKee M  Robertson A  Vaask S  Pudule I  Grinberga D  Abaravicius A  Bartkeviciute R 《Public health nutrition》2002,5(3):397-404

  相似文献   

Changes in the chemical composition of water percolating through the soil profile in a moderately polluted catchment     

R. Laskowski  M. Marýanski  M. Nklińska 《Water, air, and soil pollution》1995,85(3):1759-1764

Throughfall (TF), stemflow (SF), soil solution below the organic layer (SS_org) and at 50 cm depth (SS₅₀), and output with stream water (SW) were measured and analyzed for four years in a moderately polluted forest catchment in southern Poland. The input of water with stemflow was ca. 6% of input with TF. However, due to higher concentrations of most ions in SF, the input of most elements with SF was from 8% to 9%. Sulphate (SO₄ ^2–), chloride (Cl^–) and magnesium (Mg²⁺) were the only ions steadily increasing in concentrations in water percolating through the soil profile. Nitrogen reached the forest floor mainly as ammonium (NH₄ ⁺). In the soil organic layer the NH₄ ⁺ concentration decreased, while concentrations of nitrate (NO₃ ^–) and hydrogen (H⁺) increased, probably due to nitrification. For NO₃ ^–, sodium (Na⁺) and calcium (Ca²⁺), the highest concentrations were found in SS_org and SW. This indicates both efficient cycling in the biotic pool of the ecosystem and intensive weathering processes in the mineral soil below the plant rooting zone. The latter was especially pronounced for Mg and Ca. Concentrations of zinc (Zn), lead (Pb) and cadmium (Cd) were the highest in SS_org and SS₅₀. As this was accompanied by a low pH and constant input of H⁺, NH₄ ⁺ and heavy metal ions to the catchment area, it may pose a serious threat to forest health.  相似文献   

ADP ribosylation factor regulates metabolism and antioxidant capacity of transgenic potato tubers     

Zuk M  Prescha A  Kepczyński J  Szopa J 《Journal of agricultural and food chemistry》2003,51(1):288-294

In our recent studies we have evidenced that repression of ADP-ribosylation factor (ARF) in potato plants results in 14-3-3 gene activation. The significant alteration in plant phenotype and in carbohydrate content clearly indicates that there may also be changes in other metabolite syntheses. In this paper we present the data on contents of compounds, occurring in transgenic potato tubers from field trial, known to be important for the human diet. We also determine which of the ARF-antisense plant features resulted from ARF repression. This determination was accomplished by the analysis of ARF-antisense plants transformed with cDNA encoding 14-3-3 protein in reverse orientation. The sucrose accumulation and the decrease in glycoalkaloids level were found to be characteristic features of all transgenic plants. The increase in antioxidant capacity of transgenic potato tubers should also be pointed out. The analysis of fat from modified potato tubers revealed a nutritionally valuable composition of fatty acids, including the significant increase of linoleic acid level.  相似文献   

Differentiation of interglycosidic linkages in permethylated flavonoid glycosides from linked-scan mass spectra (B/E)     

Frański R  Matławska I  Bylka W  Sikorska M  Fiedorow P  Stobiecki M 《Journal of agricultural and food chemistry》2002,50(5):976-982

A series of per-O-methylated flavonoid di- and tri-glycosides, linked with 1-2 and/or 1-6 glycosidic bonds between sugar rings that were isolated from different plant materials were analyzed. It was demonstrated that the fragmentation behavior of permethylated flavonoid glycosides is dependent on the glycosidic bond placement between sugars. Y(n) type fragment ions, created after glycosidic bond cleavage with oxygen retention on sugar at the reducing end for permethylated compounds, were observed in the normal and linked-scan mass spectra recorded for alpha(1-2) bonded conjugates of flavonoid di- and tri-glycosides. Moreover, for alpha (1-6) linked glycosides, Y fragments created after rearrangement and elimination of internal sugar residues were observed in addition to Y(n) type ions, but these fragment ions were not registered in normal desorption ionization spectra. This second type of fragmentation was also reported previously in collision-induced dissociation tandem mass spectrometry (CID MS/MS) spectra of some oligosaccharides and flavonoid glycosides, but their presence was independent of the glycosidic bonds placement between sugar rings.  相似文献   

Serotypes of Escherichia coli strains isolated from piglets with diarrhea in swine industrial farms     

D. Ciosek  M. Truszczy&#x;ski 《Comparative immunology, microbiology and infectious diseases》1982,5(4):405-412

Serotypes of E. coli strains isolated from piglets, which died with symptoms of diarrhea in 9 swine industrial farms, were determined. Large numbers of serotypes (from 16 to 27) in individual farms were detected. The sets of serotypes from 9 investigated farms differed among each other significantly, depending on the farm and time of examination. It was found that more than one serotype of E. coli may exist in the pig body and contribute to the development of disease. The predominant serotypes, i.e. those comprising more than 10% of serologically determined strains, were found to exist in 6 of the investigated farms and not in the remaining ones. Among the predominant serotypes, particularly important seem to be strains with K88 antigen. For prophylaxis of piglet colibacteriosis in industrial farms in Poland two vaccines for sows are recommended: one containing the K88 antigen only and the other the following serotypes: 0149:K91,K88; 020:K57; 020:K83; 0157:K88; 01:K1; 0136:K78; 024:K?; 078:K80 and 0118:K? Strains belonging to these serotypes were the most prevalent in our strain collection.  相似文献