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81.
Analysis of fenbendazole residues in bovine milk by ELISA 总被引:1,自引:0,他引:1
Brandon DL Bates AH Binder RG Montague WC Whitehand LC Barker SA 《Journal of agricultural and food chemistry》2002,50(21):5791-5796
Fenbendazole residues in bovine milk were analyzed by ELISAs using two monoclonal antibodies. One monoclonal antibody (MAb 587) bound the major benzimidazole anthelmintic drugs, including fenbendazole, oxfendazole, and fenbendazole sulfone. The other (MAb 591) was more specific for fenbendazole, with 13% cross-reactivity with the sulfone and no significant binding to the sulfoxide metabolite. The limit of detection of the ELISA method in the milk matrix was 7 ppb for MAb 587 and 3 ppb for MAb 591. Fenbendazole was administered in feed, drench, and paste form to three groups of dairy cattle. Milk was collected immediately before dosing and then every 12 h for 5 days. The ELISA indicated that residue levels varied widely among individual cows in each group. Fenbendazole levels peaked at approximately 12-24 h and declined rapidly thereafter. Metabolites were detected at much higher levels than the parent compound, peaked at approximately 24-36 h, and declined gradually. Residue levels were undetectable by 72 h. The ELISA data correlated well with the total residues determined by chromatographic analysis, but the use of the two separate ELISAs did not afford an advantage over ELISA with the single, broadly reactive MAb 587. The ELISA method could be used to flag high-residue samples in on-site monitoring of fenbendazole in milk and is a potential tool for studying drug pharmacokinetics. 相似文献
82.
Chaovanalikit A Thompson MM Wrolstad RE 《Journal of agricultural and food chemistry》2004,52(4):848-852
Anthocyanins and phenolics of 10 blue honeysuckle (Lonicera caerulea L.) genotypes were characterized and quantified by HPLC-DAD. Peak assignments were confirmed by low-resolution electrospray mass spectrometry. Six anthocyanins were detected with the major peak identified as cyanidin 3-glucoside. Five additional anthocyanins were characterized as cyanidin 3,5-diglucoside, cyanidin 3-rutinoside, pelargonidin 3-glucoside, peonidin 3-glucoside, and peonidin 3-rutinoside. Four polyphenolics were identified as chlorogenic acid, neochlorogenic acid, quercetin 3-rutinoside, and quercetin 3-glucoside. Two additional unidentified phenolics were characterized as flavonol and hydroxycinnamic derivatives based on UV-vis spectra. Hydroxycinnamate levels ranged from 30.4 to 156.2 mg/100 g, whereas the flavonol content ranged from 12.6 to 32.8 mg/100 g. The L. caerulea subspecies boczkarnikovae contained the highest amounts of hydroxycinnamic derivatives and flavonols. 相似文献
83.
84.
Bunzel M Ralph J Lu F Hatfield RD Steinhart H 《Journal of agricultural and food chemistry》2004,52(21):6496-6502
Plant cell walls containing suberin or lignin in the human diet are conjectured to protect against colon cancer. To confirm the existence of authentic lignin in cereal grain dietary fibers, the DFRC (derivatization followed by reductive cleavage) method was applied to different cereal grain dietary fibers. By cleavage of diagnostic arylglycerol-beta-aryl (beta-O-4) ether linkages and identification of the liberated monolignols, it was ascertained that lignins are truly present in cereal grains. From the ratios of the liberated monolignols coniferyl alcohol and sinapyl alcohol, it is suggested that lignin compositions vary among cereals. Furthermore, dimeric cross-coupling products, comprising ferulate and coniferyl alcohol, were identified in most cereal fibers investigated. These ferulate 4-O-beta- and 8-beta-coniferyl alcohol cross-coupled structures indicate radical cross-coupling of polysaccharides to lignin precursors via ferulate. 相似文献
85.
Global climate change as currently simulated could result in the broad-scale redistribution of vegetation across the planet. Vegetation change could occur through drought-induced dieback and fire. The direct combustion of vegetation and the decay of dead biomass could result in a release of carbon from the biosphere to the atmosphere over a 50- to 150-year time frame. A simple model that tracks dieback and regrowth of extra-tropical forests is used to estimate the possible magnitude of this carbon pulse to the atmosphere. Depending on the climate scenario and model assumptions, the carbon pulse could range from 0 to 3 Gt of C yr?1. The wide range of pulse estimates is a function of uncertainties in the rate of future vegetation change and in the values of key model parameters. 相似文献
86.
The representative mass ratio of the reactive airborne sulphur-to-nitrogen, S/N in the gas and aerosol phases, and in wet and dry deposition over the territory of Hong Kong, SAR,was determined, utilizing available data for the 12 yr period, 1986–1997. Using this information, the representative stoichiometric ratio values for sulphate-to-nitrate in aerosoland in wet and dry depositions were estimated. A limited supplementary data set on various canopy depositions was alsoutilized. This information collectively suggests several basicdifferences in photochemical processes between the urbanatmospheres in and around Hong Kong and the classical, smoggyLos Angeles. This is also supported by the typical diurnal variation of nitrogen oxides in Hong Kong, which suggests a moreefficient photo-oxidation of NO to NO2 within the territoryand in nearby areas upwind, as compared to further oxidation tothe NO3 (or nitrate) stage. On the other hand, the relatively low gas-to-particle ratio value estimated for S (~3) relative to N (~26) suggests that the advectedfraction of non-sea salt aerosol sulphate in the territory couldbe considerably greater than the corresponding fraction for nitrate. Finally, significant losses (probably related to sampling) of NH4-NH3 in dry deposition could be inferred, while for SO4 in wet deposition, a moderate enrichment factor of ~2 is observed in relation to SO4 in aerosol. 相似文献
87.
Paige C. R. Snodgrass W. J. Nicholson Ronald V. Scharer J. M. He Q. H. 《Water, air, and soil pollution》1997,97(3-4):397-412
The presence of phosphate retards the transformation of ferrihydrite into crystalline products. Increasing phosphate from 0 to 1 mole % results in an order of magnitude decrease in the rate of transformation of ferrihydrite at pH 12. Levels of phosphate of ~1 mol % suppress the formation of goethite (α-FeO(OH)) and result in the formation of a product consisting ofη-Fe2O3. Higher levels of phosphate result in the ferrihydrite remaining amorphous, even after several hundred hours. Phosphate prevents formation of goethite by hindering the dissolution of ferrihydrite rather than by interfering with nucleation and growth of goethite in solution. The transformation rate of pure ferrihydrite is also strongly inhibited in the presence of dissolved phosphate. This is due to surface complexation. The transformation rate was measured at temperatures of 60 °C and 70 °C. The rate of transformation was found to be described by either (i) a solid-state reaction equation for powdered compacts or (ii) a zero-order reaction controlled by desorption. The transformation of the ferrihydrite matrix was accompanied by the loss of the phosphate trace component. X-ray diffraction indicates that no solid solution involving phosphate substitution intoη-Fe2O3 is formed. Transmission electron microphotographs of the original precipitates containing phosphate confirm the presence of the phosphate and demonstrate its involvement in linking together extremely small particles of ferrihydrite. 相似文献
88.
Sigler William V. Reicher Zachary Throssell Clark Bischoff Marianne Turco Ronald F. 《Water, air, and soil pollution》2003,142(1-4):311-326
Microbial degradation of fungicides on leaf surfaces after repeated applications to turfgrass was investigated. Prior andcurrent work in our laboratory has identified two characteristicsof the turfgrass leaf system that may contribute to the enhanceddegradation of fungicides after repeated application to turfgrass:(1) The leaf surface is rich in microorganisms (~108 g-1 dr wt leaf), and (2) Leaf surface microorganisms may respond to repeated fungicide applications in a manner consistentwith the phenomena of enhanced biodegradation. Field studies wereconducted on ‘Penncross' creeping bentgrass with four fungicidesrepresenting three chemical families applied either two or eight times in one growing season. Biodegradation was estimated using data from both a field study and a parallel laboratory study thatfollowed the fate of 14C-labelled fungicides. For the laboratory incubations, the locations of the residual 14C fungicides were estimated using a sequential extraction protocolthat fractionated the materials into three pools: available,retained and bound. Data from both the field and laboratory studyrefuted our hypothesis that enhanced biodegradation would developfollowing repeated applications of the fungicides onto the leafsurface. Our studies support a conclusion that a two-stagephysical sorption process leads to plant incorporation and thiscontrols most of the fungicide's fate. Thus, our data suggestthat microbial activity plays a less important part in theprocess than would be indicated by considering the size of themicrobial population on the leaves. 相似文献
89.
Orwell Ralph L. Wood Ronald L. Tarran Jane Torpy Fraser Burchett Margaret D. 《Water, air, and soil pollution》2004,157(1-4):193-207
Water, Air, &; Soil Pollution - The quality of the indoor environment has become a major health consideration, since urban-dwellers spend 80-90% of their time indoors, where air pollution can be... 相似文献
90.
Togo Tsuji Austin Mambo Lackson K. Phiri Ronald Msoni Sesele B. Sokotela Olusegun A. Yerokun 《Soil Science and Plant Nutrition》2005,51(7):935-942
Total sulphur content of some benchmark soils was evaluated by correlating with the contents of soil organic C and amorphous oxides (Al and Fe), and the particle size distribution. An equation model to calculate the content of total soil sulphur was first obtained by regression analysis. Introducing these chemical and physical data of all the map units except for the wetlands into the equation model, the sulphur level on a national scale was estimated for each soil mapping unit in the exploratory soil map of Zambia. Second, the total sulphur content was rated into 3 classes based on the frequency distribution of the total sulphur content in the benchmark soils. Finally, the rated total sulphur content was incorporated into the national soil map database of GIS to produce a map with the total sulphur content of Zambian soils. The extent of each class was calculated and the distribution of the areas was examined in relation to rainfall and soil formation. 相似文献