Contagious bovine pleuropneumonia (CBPP) caused by vaccine strain T1/44 of Mycoplasma mycoides subsp. mycoides SC     

Mbulu RS  Tjipura-Zaire G  Lelli R  Frey J  Pilo P  Vilei EM  Mettler F  Nicholas RA  Huebschle OJ 《Veterinary microbiology》2004,98(3-4):229-234

A study was carried out on four adult cattle to assess the pathogenicity of Mycoplasma mycoides subsp. mycoides SC strain T1/44, currently used as a vaccine for the control of contagious bovine pleuropneumonia (CBPP) in Namibia. Post mortem examination 9 weeks after endobronchial inoculation of the vaccine strain to three of the four animals revealed unilateral pleuropneumonic lesions, pleuritis and well-developed sequesters in two of the three inoculated animals and several small sequesters surrounded by pleuropneumonic lesions in the diaphragmatic and apical lobes in one animal. The fourth animal, which was not directly inoculated but was in close contact with the inoculated animals, revealed only an adhesion area of the lung to the ribcage. Serological examination carried out using the complement fixation test (CFT) detected positive titres in all three intubated animals and the indirect CBPP-LppQ-ELISA was positive for two of the three inoculated animals. The contact animal showed no seroconversion. M. mycoides subsp. mycoides SC was isolated from the sequesters of two of the inoculated animals. Isolation of mycoplasmas was not possible from the third inoculated animal due to heavy contamination of the samples by other bacteria, but the presence of M. mycoides subsp. mycoides SC could be evidenced by PCR from clinical samples. The identity of the T1/44 vaccine strain isolated from the sequesters of two animals was confirmed by T1/44-specific PCR analysis and by IS1296 typing using Southern blot. These results clearly show that inoculation of T1/44 vaccine via the endobronchial route can lead to CBPP.  相似文献   

Use of thromboelastography in dogs with immune-mediated hemolytic anemia: 39 cases (2000–2008)     

Virginia B. Sinnott  DVM    Cynthia M. Otto  DVM  PhD  DACVECC 《Journal of Veterinary Emergency and Critical Care》2009,19(5):484-488

Objective – To analyze thromboelastograms (TEGs) of naturally occurring cases of immune-mediated hemolytic anemia (IMHA) in order to identify whether a hypercoagulable state was present and whether its presence was associated with differences in survival.
Design – Retrospective study spanning January 2000 to June 2008. Medical records of dogs were evaluated. Endpoints were considered death or discharge from the hospital.
Setting – Academic teaching hospital.
Animals – Thirty-nine dogs with a diagnosis of IMHA and at least one TEG performed during hospitalization were included.
Interventions – None.
Measurements and Main Results – Four values were evaluated from the TEG: the R time (R), K time (K), alpha angle (α), and maximum amplitude. From these values, a coagulation index (CI) was calculated to classify patients as normocoagulable, hypercoagulable, or hypocoagulable. Thirty-three of 39 patients were hypercoagulable based on the CI. The 6 remaining dogs were normocoagulable. The patients with a normocoagulable CI had an increased mortality rate (100%) when compared with the hypercoagulable patients using Fisher's exact test ( P =0.02). Additionally, prolongation of partial thromboplastin time did not preclude hypercoagulable TEG values.
Conclusions – The majority of dogs with IMHA were hypercoagulable as measured by TEG. A normal CI was associated with a worse outcome in this patient population. TEG may provide additional and complementary information to prothrombin time and partial thromboplastin time relating to coagulation status in dogs with IMHA and may help predict prognosis and potentially guide clinical decisions to utilize anticoagulant drugs.  相似文献   

Medical surveillance of search dogs deployed to the World Trade Center and Pentagon: 2001-2006     

Cynthia M. Otto  Amanda B. Downend  George E. Moore  Joanne K. Daggy  D. Lauren Ranivand  Jennifer A. Reetz  Scott D. Fitzgerald 《Journal of Veterinary Behavior: Clinical Applications and Research》2009,4(6):241-242

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DNA barcoding reveals clear delineation between spawning sites for neritic versus oceanic fishes in the Gulf of Mexico     

Makenzie Burrows  Jeremy S. Browning  Mya Breitbart  Steven A. Murawski  Ernst B. Peebles 《Fisheries Oceanography》2019,28(2):228-239

We combined research‐vessel cruises of opportunity with DNA barcoding to survey planktonic, percomorph fish eggs at 40 stations distributed across and around the Gulf of Mexico (GoM). The objectives were (a) to determine whether eggs of fishes that are potential candidates for the daily egg production method (DEPM) can be readily barcoded, (b) to identify taxa that are spawning in the GoM, (c) to determine encounter rates for eggs of economically valuable taxa, and (d) to characterize individual egg taxa as being primarily neritic, primarily oceanic, or primarily mixed (i.e., both neritic and oceanic). Of the 1,144 eggs that were individually barcoded, 709 (62%) were definitively identified to species (62 species from 42 families), with an additional 20 taxa identified to genus or subfamily level. The eggs of 15 economically important species were identified, most of which had intermediate encounter rates and moderately dispersed spatial distributions, as indicated by an index of aggregation. SIMPROF analysis of stationwise cluster analysis identified eight significant groups within the 35 stations that yielded percomorph eggs; a corresponding specieswise analysis identified six groups of stations as having a neritic egg community and two groups as having an oceanic community, with a community transition located at the shelf break. Although the neritic and oceanic stations did not share important species, it remains possible that coastal pelagic species have mixed neritic–oceanic distributions. Together, these results indicate DEPM fish‐egg surveys based on DNA barcoding are feasible at the large marine ecosystem scale.  相似文献   

Detection of CNS and PrPSc in meat products     

Lücker E  Hardt M  Groschup MH 《Berliner und Münchener tier?rztliche Wochenschrift》2002,115(3-4):111-117

Several methods for the detection of tissues of the central nervous system (CNS) in meat products have been developed and partly validated for use in official food control as pertaining to human BSE-exposure risk. So far, however, methods for the detection of abnormal prion protein (PrPSc) were not evaluated for their potential applicability to the matrix of heat treated meat products. We developed a micro technological procedure for the preparation of meat products suitable for high security laboratories as masses were 6 to 8 orders of magnitude lower than in conventional meat technology. Thus it was possible to produce standard micro sausages containing defined amounts of bovine BSE-positive brain. This material showed all characteristics of normal meat products and a homogeneous distribution of brain as indicated by NSE and GFAP western immunoblotting and GFAP immunometric analyses. Using a commercially available and certified immunometric assay for detection of PrPSc in untreated brain it was possible to detect BSE-positive CNS down to a content of 0.25% in heat treated meat products. We found a high correlation between PrPSc OD-values and CNS content and linearity up to 10% CNS. In 30 samples of retail meat products no sample transgressed the official cut off value for untreated bovine brain. Further studies are needed to show whether an increase of sensitivity in PrPSc detection from the meat product matrix is possible, in particular by optimisation of the extraction procedure.  相似文献