西藏豆科牧草青贮饲料中耐低温优良乳酸菌的筛选     

杨晓丹  原现军  郭刚  崔棹茗  李君风  白晰  巴桑  邵涛 《草业学报》2015,24(6):99-107

本研究对西藏地区豆科牧草青贮饲料中的乳酸菌进行分离、鉴定,以期得到优良的乳酸菌菌株。以苜蓿和箭筈豌豆青贮饲料为试验材料,共分离得到6株同型发酵乳酸菌,经传统微生物培养和16S rRNA序列分析,3株(LCG9,CG35和AG11)为戊糖片球菌,2株(LCG3和LAG1)为植物乳杆菌,1株(LA3)为弯曲乳杆菌;除AG11和LAG1在5℃,LCG9和AG11在pH 3.0和8.0生长微弱外,其余乳酸菌菌株均在5~20℃,pH 3.0~8.0及3.0%和6.5% NaCl培养液中生长良好。从耐低温特性、产酸能力和发酵速率3个指标考虑,植物乳杆菌LCG3最适宜用于西藏青贮饲料生产的乳酸菌菌种。  相似文献   

2种不育剂和1种抗凝血剂对藏北草原高原鼠兔的控制效果     

李波  桑珠  许军基  徐正刚  赖新  彭真  沈果  周训军  扎西  张美文 《植物保护》2015,41(6):230-234

2012年4月至2013年4月在藏北草原进行了炔雌醚、莪术醇和增效敌鼠钠盐控鼠试验。均配制成青稞药饵,投放药饵后第20、75和350天进行控效调查。结果显示:炔雌醚具杀灭高原鼠兔作用,20d校正灭效达72.24%,对成年雌雄鼠繁殖器官均能起到明显损坏作用,且适口性最好;增效敌鼠钠盐控鼠效果最好,20d校正灭效达94.46%;莪术醇也有一定效果,75d幼体比最低,总体看,3种药物均具有一定控制效果,有一定实际应用潜力,但还需进一步试验验证其控鼠效果。  相似文献   

Fumigant toxicity of basil oil compounds and related compounds to Thrips palmi and Orius strigicollis          下载免费PDF全文

Kwang‐Ho Kim  Chang‐Geun Yi  Young‐Joon Ahn  Soon Il Kim  Sang‐Guei Lee  Jun‐Ran Kim 《Pest management science》2015,71(9):1292-1296

  相似文献   

Pasting properties of colored rice starch by ball-mill treatment     

Sang Kuk Kim  Hak Yoon Kim 《Journal of Crop Science and Biotechnology》2014,17(3):161-166

Physically modified rice starch by ball-mill is important for making good quality bread, noodles, and several processed foods. The production of rice flour was done by ball-mill in an industrial scale. The effects of ball-mill treatment on the physicochemical properties of rice starch were investigated. The gel pasting properties of Joseongheugchal employed to ball-mill treatment showed that pasting time was 0.44 min shorter than that of the control and it also decreased peak viscosity (PKV), hot peak viscosity (HPV), and cold paste viscosity (CPV). In Hongjinju rice, RVA (rapid visco analyzer) pasting trace was characterized with high peak viscosity (3,448 RVU: rapid visco unit), and cold paste viscosity (2,975 RVU), also with high breakdown viscosity (1,597 RVU). The onset, peak, and conclusion gelatinization temperatures of ball-milled Josengheugchal rice starch were 57.4, 64.2, and 76.5°C, respectively, which were significantly higher than those of the control. The parameters of DSC (Differential scanning calorimetry) of ball-milled Hongjinju rice were 57.9, 65.1, and 77.3°C, respectively. The mean particle size of starch in both Josengheugchal and Hongjinju by ball-milling was decreased. The distribution of mean particle size in ball-milled rice starch was smaller in Josengheugchal than in Hongjinju compared to the native starch. As rice grains were ball-milled, the regions of amorphism were becoming larger and larger at the expense of crystalline regions, and diffraction peak patterns were decreased commonly in two colored rice cultivars. The ¹³C FT-solid state NMR patterns of starches showed dramatic chemical shifts of the ¹³C resonances by ball-milled treatment.  相似文献   

西藏不同饲草全混合日粮发酵品质和有氧稳定性的研究     

王勇  原现军  郭刚  闻爱友  王坚  肖慎华  余成群  巴桑  邵涛 《草业学报》2014,23(6):95-102

为了充分利用西藏的农作物秸秆和饲草资源,本试验在当地奶牛场采用的以全株玉米和青稞秸秆为主要粗饲料的TMR配方基础上,用苜蓿、全株燕麦和小麦秸秆按不同比例替代全株玉米。青贮发酵45 d后评定各组发酵品质、营养特性和有氧稳定性。结果表明,发酵45 d后,TMR-2、TMR-3和TMR-4组pH值显著高于TMR-1(P<0.05),乳酸含量均低于TMR-1,但各替代组仅检测到微量丙酸和丁酸含量,且氨态氮/总氮较低,表明各组发酵品质均良好。发酵后各组水溶性碳水化合物含量均有大幅度下降,除TMR-1组外,其他3组干物质含量基本不变,粗蛋白、中性洗涤纤维、酸性洗涤纤维和粗脂肪等营养物质含量不同程度增加,其中TMR-2和 TMR-3组粗蛋白含量高于15%,能够满足改良奶牛的营养需要。在有氧暴露过程中,各组乳酸、乙酸和水溶性碳水化合物含量均出现不同程度的下降,丙酸和丁酸含量在有氧暴露第3天均有升高,之后逐渐下降。在有氧暴露9 d中,pH值均呈上升趋势,其中TMR-3组pH值上升幅度最小,其有氧稳定性最好。综上所述,虽然TMR-3发酵品质略低于TMR-1组,但其有氧稳定性有所提高,因此,TMR-3组可应用于生产实际中。  相似文献   

添加乙酸对西藏燕麦和紫花苜蓿混合青贮发酵品质和有氧稳定性的影响   总被引：3，自引：0，他引：3  

李君风  孙肖慧  原现军  郭刚  肖慎华  巴桑  余成群  邵涛 《草业学报》2014,23(5):271-278

为探讨不同水平乙酸对燕麦和紫花苜蓿(7∶3)混合青贮发酵品质和有氧稳定性的影响,试验设对照组和3个乙酸添加组(0.3%,0.4%,0.5%),青贮后第30,45和60天开窖取样,测定青贮饲料的发酵品质,同时60 d后打开剩余的所有实验室青贮窖,将青贮饲料暴露于空气中,测定青贮饲料有氧稳定性。结果表明,添加乙酸显著(P<0.05)降低了混合青贮饲料的pH,其中0.4%与0.5%乙酸处理组pH显著(P<0.05)低于0.3%乙酸处理组。青贮60 d后乙酸添加组乳酸含量显著(P<0.05)高于对照组,但处理组间差异不显著(P>0.05)。添加乙酸显著(P<0.05)降低了青贮过程中氨态氮/总氮,且氨态氮/总氮随着乙酸添加量的增加呈下降趋势(P>0.05)。各乙酸添加组水溶性碳水化合物均显著(P<0.05)高于对照组,其中0.4%乙酸添加组最高。在有氧暴露前10 d,各组pH和氨态氮/总氮基本保持稳定,其中乙酸添加组低于对照组。暴露10 d后各组pH均有上升,其中对照组显著(P<0.05)上升。在整个有氧暴露阶段,乳酸和水溶性碳水化合物含量逐渐下降,但各乙酸添加组均显著(P<0.05)高于对照组,其中0.4%乙酸添加组乳酸含量始终保持最高。综合考虑发酵品质和有氧稳定性,0.4%乙酸添加量最适宜。  相似文献   

幼羔超早期繁殖技术研究   总被引：2，自引：0，他引：2  

陈晓勇  敦伟涛  田树军  桑润滋  孙洪新  张建鑫  孙树春 《中国农学通报》2010,26(2):21-25

幼羔超早期繁殖技术是集幼羔超排、活体采卵、体外受精、胚胎移植等技术为一体的胚胎生物技术体系。诱导羔羊卵泡发育技术（简称“幼羔超排”）是根据性成熟前幼龄母羔（1～2月龄）卵巢对生殖激素敏感,卵泡发育很少发生闭锁的特殊生理特点而发明的一项高效率生产羊卵母细胞的新技术。本文从幼羔超早期利用的意义,幼羔超排、幼羔卵母细胞体外受精和胚胎移植等方面综述了目前幼羔超早期利用技术的现状及发展前景。  相似文献   

Effect of Docosahexaenoic Acid on Quality of Cryopreserved Boar Semen in Different Breeds     

K Kaeoket  P Sang‐urai  A Thamniyom  P Chanapiwat  M Techakumphu 《Reproduction in domestic animals》2010,45(3):458-463

During the cryopreservation process, the level of polyunsaturated fatty acids, especially docosahexaenoic acid (DHA), in the sperm plasma membrane decreases significantly because of lipid peroxidation, which may contribute to sperm loss quality (i.e. fertility) of frozen–thawed semen. The aim of this study was to investigate the effect of supplementation of DHA (fish oil) in freezing extender II on frozen–thawed semen quality. Semen from 20 boars of proven motility and morphology, were used in this study. Boar semen was split into four groups, in which the lactose–egg yolk (LEY) extender used to resuspend the centrifuged sperm pellet was supplemented with various levels of fish oil to reach DHA level of 1X (group I, control, no added fish oil), 6X (group II), 12X (group III) and 18X (group IV). Semen solutions were frozen by using a controlled rate freezer. After cryopreservation, frozen semen was thawed and evaluated for progressive motility, viability by using SYBR‐14/Ethidiumhomodimer‐1 (EthD‐1) staining and acrosome integrity by using FITC‐PNA/EthD‐1 staining. There was a significantly higher (p < 0.001) percentage of progressive motility, viability and acrosome integrity in DHA (fish oil) supplemented groups than control group. Generally, there seemed to be a dose‐dependent effect of DHA, with the highest percentage of progressive motility, viability and acrosome integrity in group‐III. In conclusion, supplementation of the LEY extender with DHA by adding fish oil was effective for freezing boar semen as it resulted in higher post‐thaw plasma membrane integrity and progressive motility.  相似文献   

微波加热对农产品营养和风味的影响   总被引：1，自引：0，他引：1  

王静  桑俊利 《农产品加工．学刊》2010,(1):53-55

营养和风味是农产品的重要特征。详细介绍了微波加热对农产品营养和风味影响的研究进展。结果表明,与传统方法相比,微波加热更有利于农产品中蛋白质、脂肪、碳水化合物,以及维生素等营养成分的保存,而且在微波加热过程中,蛋白质、脂肪、碳水化合物,以及维生素等营养成分也会对农产品风味产生一定的影响。  相似文献   

Multidetector row computed tomography evaluation of the micropig kidney as a potential renal donor     

Woong Yoon  Min Young Lee  Jung Min Ryu  Yong Ju Moon  Sang Hun Lee  Jae Hong Park  Seung Pil Yun  Min Woo Jang  Sung Su Park  Ho Jae Han 《Journal of veterinary science (Suw?n-si, Korea)》2010,11(1):9-13

Multidetector row computed tomography (MDCT) provides anatomical information about the kidney and other internal organs. Presently, the suitability of 64-channel MDCT to assess the kidney of healthy micropigs was evaluated. Morphological evaluations of the kidney and the major renal vessels of six healthy micropigs were carried out using MDCT, recording kidney volume and the diameter and length of renal arteries and veins. The mean diameters and lengths of the renal artery were 0.44 ± 0.05 and 4.51 ± 0.55 cm on the right side and 0.46 ± 0.06 and 3.36 ± 0.27 cm on the left side, respectively. The mean diameters and lengths of the renal vein were 1.44 ± 0.52 and 4.22 ± 1.29 cm on the right side and 1.38 ± 0.17 and 5.15 ± 0.87 cm on the left side, respectively. The mean volume of the right kidney was 79.3 ± 14.5 mL and of the left kidney was 78.0 ± 13.9 mL. The data presented in this study suggest that the MDCT offers a noninvasive, rapid, and accurate method for the evaluation of the renal anatomy in living kidney donors. It also provides sufficient information about extra-renal anatomy important for donor surgery and determination of organ suitability.  相似文献