不同养分进食水平及日粮降解蛋白质与代谢能比对妊娠后期细毛羊生产性能的影响     

李建国  桑润滋  曹玉凤  郭钢  孔祥浩  王俊杰 《动物营养学报》1996,(2)

用87只2～3岁的河北细毛羊母羊,对日粮不同代谢能(ME)、降解蛋白(RDP)和非降解蛋白(UDP)水平对妊娠后期母羊体重及羔羊初生重影响进行了研究。试验结果表明,河北细毛羊妊娠后期(100～150天)能量和蛋白质的适宜供给量分别是0.79MJME/kgW~(0.75)/d、7.02gRDP/kgW~(0.75)/d和2.32gUDP/kgW~(0.75)/d。这一营养水平可保证母羊体重适度增加。同时还发现母羊日粮能量及蛋白质水平对羔羊初生重无显著影响。  相似文献   

Enhancement of Campylobacter jejuni virulence by serial passage in chicks     

F C Sang  S M Shane  K Yogasundram  H V Hagstad  M T Kearney 《Avian diseases》1989,33(3):425-430

Four isolates of Campylobacter jejuni were studied to determine changes in virulence following six serial passages in chicks. Chicks that received invasive isolates exhibited diarrhea and depressed weight gain. Immature mice were used to assess virulence of the passaged isolates of C. jejuni. Nine-day-old mice infected with passaged isolates showed lethargy, dehydration, depression, decreased weight gain, and occult blood in feces. Mouse pups inoculated with the third and sixth chick passage levels of an invasive isolate showed significant depression in mean daily weight gain and elevated mortality compared with controls and subjects inoculated with unpassaged isolates. This study demonstrated enhancement of virulence in a C. jejuni isolate following chick passage. In contrast, three other passaged isolates failed to show any consistent increase in virulence.  相似文献   

The phagocytic cell response of the ovine lung to Pasteurella haemolytica     

D.H. Davies  Rosemary A. Penwarden 《Veterinary microbiology》1981,6(3):183-189

Conventionally-reared sheep were inoculated with (3.0 ± 0.6 × 10⁷) viable Pasteurella haemolytica type A1 by the intratracheal route and were killed immediately (0-time) or 2, 4, 12, 16, 24, 48 or 72 h later. Lung-wash cells and free bacteria were recovered by pulmonary layage.The number of recoverable bacteria tended to increase between 0-time and 4 h post-in-oculation (p.i.) then decline rapidly over the next 8 h. However, the rate of clearance was extremely variable and viable bacteria were recovered from $\text{3}\text{5}$ animals at 48 h p.i. and from $\text{1}\text{5}$ at 72 h p.i.In parallel with the clearance of the majority of the bacteria, total neutrophil numbers in the lung-wash rose to a peak of (36 ± 6) × 10⁸ cells/lung, which was, on average, 70-fold higher than 0-time levels. Their numbers remained constant from 12 to 24 h p.i. then fell to be 5-fold above 0-time levels at 72 h p.i. Macrophage numbers rose slowly throughout the experiment but most of the increase occurred between 24 and 48 h p.i. They reached a peak of (17 ± 11) × 10⁸ cells/lung at 48 h i.p. which was 3-fold higher than 0-time levels.  相似文献   

Survey of line crosses in a Brown Leghorn flock. II. Relations of hatched chick weight of egg weight in inbred lines and their crosses     

J S Blyth  C F Pun  J H Sang 《British poultry science》1965,6(3):217-223

A preliminary study of the hatching weight of chicks of two pairs of pure lines and their reciprocal crosses shows that the relationship of net chick weight, excluding yolk sac, to egg weight, is constant for the lines ; although this is not so for an exceptional line selected for small size, but not examined in detail. Chicks from one pair of reciprocal matings are relatively larger than representatives of their parent lines, but those from the other are not.

Detailed examination of the amount of egg material used to form the chick shows that two lines which produce the same relative size of chick do so because one line uses more of the egg, but utilises it less efficiently, than the other. Further, the different genotypes take different times to hatch, the hybrids being earlier than their corresponding dam line. There are differences between reciprocals in hatching time and in efficiency of egg utilisation, but they tend to be confounded. There is no evidence of an optimal egg size for relative chick weight either within lines or within hybrid groups.  相似文献   

动物园的科学发展观与市场化选择     

刘小青  桑青芳  严志强 《野生动物》2006,27(2):53-56

本文从动物园的发展实践中，提出动物园的科学发展观；从发展机遇、体制创新、市场定位、功能发挥、文化建园、开拓市场、科学管理和依法经营8个方面论述了市场经济对我国动物园提出的要求。  相似文献   

Evaluation of equine papillomas, aural plaques, and sarcoids for the presence of Equine papillomavirus DNA and Papillomavirus antigen          下载免费PDF全文

Rosemary C. Postey  Greg D. Appleyard    Beverly A. Kidney 《Canadian journal of veterinary research》2007,71(1):28-33

Immunohistochemical (IHC) testing and electron microscopy have implicated Papillomavirus (PV) as the etiologic agent for equine papillomas and aural plaques, but Equine papillomavirus (EPV) DNA has yet to be demonstrated in these lesions by polymerase chain reaction (PCR). The purpose of this study was to evaluate formalin-fixed, paraffin-embedded tissues from naturally occurring cases of equine papillomas, aural plaques, and sarcoids for the presence of EPV DNA by means of PCR and for the presence of PV antigen by means of IHC testing. We used EPV-specific primers that amplified a region of 384 base pairs (bp) spanning the E4 and L2 genes of the EPV genome and consensus PV primers that amplified a 102-bp region of the L1 gene. Group-specific PV structural antigens were detected with the use of a streptavidin-biotin-alkaline phosphatase IHC stain. With IHC testing, 23 of 38 papillomas, 4 of 9 aural plaques, and 0 of 10 sarcoids were positive for PV antigen; EPV DNA was found in 20 of the 38 papillomas and 1 of the 10 sarcoids but 0 of the 9 aural plaques. The consensus primers did not amplify novel PV DNA in any of the tissues. Nucleotide sequencing of viral DNA from 7 papillomas amplified with EPV-specific primers revealed DNA fragments that were 96% to 99% identical to known EPV sequences. Some samples had nucleotide substitutions in common, which suggests infection with related strains. Together, EPV DNA or PV antigen (or both) was demonstrated in 26 (68%) of the 38 equine papillomas. Although aural plaques contained PV antigen, they were negative for EPV DNA; therefore, we hypothesize that aural plaques contain a PV distinct from EPV.  相似文献   

Long-term evaluation of canine perianal fistula disease treated with exclusive fish and potato diet and surgical excision     

Lombardi RL  Marino DJ 《Journal of the American Animal Hospital Association》2008,44(6):302-307

The records of 33 dogs that had perianal fistula disease treated with en bloc surgical excision and bilateral anal saculectomy, and that were perioperatively administered an exclusive white fish and potato diet, were reviewed to determine outcome. By 1 year after surgery, 87.9% of the dogs had complete or near-complete resolution of visible fistula disease, while only 20.7% continued to have mild intermittent clinical signs. Fecal incontinence was not reported in any dog. Overall, complications were considerably less in both severity and frequency when compared with previous reports.  相似文献   

Assessment of antibiotic resistance phenotype and integrons in Salmonella enterica serovar Typhimurium isolated from swine     

Rayamajhi N  Kang SG  Kang ML  Lee HS  Park KY  Yoo HS 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(10):1133-1137

Salmonella enterica serovar Typhimurium (S. Typhimurium) isolated and identified from swine were subjected for the analysis of antibiotic resistance pattern and clinically important class 1 and 2 integrons. In addition, S. Typhimurium isolates exhibiting ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, tetracycline and florfenicol (ACSSuTF) resistance pattern as described in most Salmonella enterica serotype Typhimurium definitive type 104 (DT104) were characterized by polymerase chain reaction. All the isolates were resistant to more than four antibiotics and showed the highest resistance to streptomycin (94.1%), followed by tetracycline (90.1%), ampicillin (64.7%), chloramphenicol (56.8%) and gentamicin (54.9%). MIC value for the ten isolates ranged between 0.125-2 mug/ml for ciprofloxacin. Among the beta-lactams used, only one of the isolate exhibited resistance to ceftiofur (MIC 8 microg/ml). Sixty eight percent of these multi drug resistance (MDR) S. Typhimurium isolates carried clinically important class 1 integron with 1kb (aadA) and/or 2kb (dhfrXII-orfF-aadA2) resistance gene cassettes. This study reports the increasing trend of multi drug resistance (MDR) S. Typhimurium with clinically important class 1 integron in pigs. In addition, emergence of the ACSSuTF-type resistance in S. Typhimurium PT other than DT104 may limit the use of resistance gene markers in its detection methods by PCR.  相似文献   

Characteristics of persistent Salmonella Enteritidis strains in two integrated broiler chicken operations of Korea     

Kwag SI  Bae DH  Cho JK  Lee HS  Ku BG  Kim BH  Cho GJ  Lee YJ 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(10):1031-1035

The objectives of the study were to investigate the phenotypic and genotypic characterization of the persistent Salmonella Enteritidis (S. Enteritidis) isolates in two integrated broiler chicken operations, with attention focused mainly on the epidemiological approach. In the distribution of virulence genes, Salmonella enterotoxin (stn), invading host cell (invA), and Salmonella plasmid virulence (spvC) genes were widely distributed among the S. Enteritidis irrespective of their source of isolation, and Salmonella fimbrial (sefC) and plasmid encoded fimbrial (pef) genes were present in 28 and 20 S. Enteritidis strains, respectively. A total of 5 different XbaI-PFGE types were obtained from 31 S. Enteritidis isolates. Twenty-one types were divided on the basis their PFGE pattern, phage type and antimicrobial resistance pattern determined. There was a significant difference in phenotypic and genotypic characterization by two integrated broiler operations. Also, 8 isolates shown susceptible to all antimicrobials and 11 isolates with resistance to nalidixic acid were partly classified by XbaI PFGE pattern and by the phage type.  相似文献   

A novel balloon technique to induce intra‐abdominal hypertension and its effects on cardiovascular parameters in a conscious dog model          下载免费PDF全文

Min Jang DVM  PhD  Won‐gyun Son DVM  PhD  Sang‐min Jo DVM  Hyunseok Kim DVM  Chi Won Shin DVM  Inhyung Lee DVM  MS  PhD 《Journal of Veterinary Emergency and Critical Care》2018,28(4):326-333

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