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51.
Summary The EVIKA Research Centre has developed a disease eradication system for the initial material of seed potato, which consists
of 1. Selection of the initial material for eradication, plant thermotherapy, meristem tip cultivation and testing for viruses;
2. Re-eradication (thermotherapy of test-tube plants, cultivation of meristem tips, testing for viruses, testing of varietal
quality and yield of disease-free meristem clones, and the selection of meristem clones with best varietal characteristics;
3. Renewing of the initial material (all processes as above, but the initial material is selected from the seed potatoes grown
for at least 3 years in the field).
Field trials were conducted after the second cycle of eradiaction to determine whether the plantlets were true-to-type and
to assess the yield, disease resistance and tuber dry matter. Recently we have also started in vitro testing of meristem plants
resistance to late blight, hoping that this approach will improve the selection efficiency.
Propagation and growing of the seed tubers consists of 3 main steps: 1. Plantlet propagation in vitro; 2. Greenhouse propagation
in plastic rolls; and 3. Planting the first generation tubers in the field. Under our conditions it is the cheapest, simplest
and most effective way.
Multiplication of the meristem-derived plants is done by shoot tip cuttings in a greenhouse in plastic rolls with peat as
the growing medium. The first generation of seed tubers is grown in the field. In our trials plant productivity was affected
by the method of multiplication, growing conditions and genotype. The highest yield per hectare was obtained with plants multiplied
in plastic rolls. The in vitro plants had more tubers per plant than plants multiplied in plastic rolls. In seed production
fields the productivity of plants was more influenced by following our instructions than by multiplication and growing methods.
The number of tubers per plant obtained with over 0.5 million plants was 6.5–9.0.
In the second generation of seed tubers, grown under equal density, the greater-sized tubers gave more tubers with lower weight
than the smaller-sized tubers. Cutting tubers had no effect on the plant development and tuberization. 相似文献
52.
Clinical efficacy and safety of a once-daily formulation of carbimazole in cats with hyperthyroidism
R. Frénais D. Rosenberg S. Burgaud L. J. I. Horspool 《The Journal of small animal practice》2009,50(10):510-515
O bjective : Evaluation of efficacy and safety of a novel controlled-release formulation of carbimazole in feline hyperthyroidism.
M ethods : A multicentre, self-controlled study in 44 client-owned cats with history and clinical signs of hyperthyroidism, and total thyroxine concentration greater than or equal to 50 nmol/l. Treatment was started at 15 mg once daily, response assessed after 10 days, and 3, 5, 8, 26 and 53 weeks and dose adjusted as required.
R esults : The median dose of carbimazole was 10 mg (range 10 to 15 mg) and 15 mg (5 to 25 mg) once daily after 3 and 53 weeks, respectively. Median total thyroxine concentration dropped significantly from 118 nmol/l (50 to 320 nmol/l) at presentation to 33 nmol/l (n=40) after 10 days, 31 nmol/l (n=34) at 3 weeks and 21 nmol/l (n=18) at 53 weeks. Clinical signs improved or resolved in almost all cats within three weeks after starting treatment. Twenty-one adverse reactions possibly (20) or probably (1) related to treatment were reported. During treatment, increased blood urea nitrogen concentration was observed in 25 per cent of the cats, eosinophilia in 20 per cent and lymphopenia in 16 per cent, while liver enzymes tended to improve.
C linical S ignificance : Once daily administration of controlled-release carbimazole tablets was effective and had expected tolerance in hyperthyroid cats during short- and long-term treatment. 相似文献
M ethods : A multicentre, self-controlled study in 44 client-owned cats with history and clinical signs of hyperthyroidism, and total thyroxine concentration greater than or equal to 50 nmol/l. Treatment was started at 15 mg once daily, response assessed after 10 days, and 3, 5, 8, 26 and 53 weeks and dose adjusted as required.
R esults : The median dose of carbimazole was 10 mg (range 10 to 15 mg) and 15 mg (5 to 25 mg) once daily after 3 and 53 weeks, respectively. Median total thyroxine concentration dropped significantly from 118 nmol/l (50 to 320 nmol/l) at presentation to 33 nmol/l (n=40) after 10 days, 31 nmol/l (n=34) at 3 weeks and 21 nmol/l (n=18) at 53 weeks. Clinical signs improved or resolved in almost all cats within three weeks after starting treatment. Twenty-one adverse reactions possibly (20) or probably (1) related to treatment were reported. During treatment, increased blood urea nitrogen concentration was observed in 25 per cent of the cats, eosinophilia in 20 per cent and lymphopenia in 16 per cent, while liver enzymes tended to improve.
C linical S ignificance : Once daily administration of controlled-release carbimazole tablets was effective and had expected tolerance in hyperthyroid cats during short- and long-term treatment. 相似文献
53.
54.
Organogenesis and foetal haemodynamics during the normal gestation of healthy black‐rumped agoutis (Dasyprocta prymnolopha,Wagler, 1831) bred in captivity 下载免费PDF全文
FCA Sousa GT Pessoa LS Moura JR Araújo RPS Rodrigues MAPS Barbosa AN Diniz AB Souza EG Silva LU Lucena MP Sanches OF Silva‐Filho PC Guerra JM Sousa WC Neves FR Alves 《Reproduction in domestic animals》2017,52(1):60-66
The objective of this study was to define the patterns of organogenesis and foetal haemodynamics during the normal gestation of healthy agoutis (Dasyprocta prymnolopha) kept in captivity. Thirty pregnant agoutis that ranged in size from small to medium and weighed between 2.5 and 3 kg underwent B‐mode and Doppler ultrasonography for the biometric evaluation of the foetal organs. The foetal aortic blood flow proved to be predominantly systolic, and the measured flow velocity was 78.89 ± 2.95 cm/s, with a maximum pressure gradient of 2.12 ± 0.27 mmHg. The liver was characterized by its large volume, occupying the entire cranial aspect of the abdominal cavity, and it was associated cranially with the diaphragm and caudally with the stomach. The flow velocity in the portal vein was estimated to equal 12.17 ± 2.37 cm/s, with a resistivity index of 0.82 ± 0.05. The gallbladder was centrally located and protruded cranially towards the diaphragm. The spleen was visualized as an elongated structure with tapered cranial and caudal extremities, and the foetal kidneys were visualized bilaterally in the retroperitoneal region, with the right kidney positioned slightly more cranially than the left. The morphological characterization and hemodynamic analysis of the foetal organs of black‐rumped agoutis via B‐mode and Doppler ultrasonography allow determination of the vascular network and of reference values for the blood flow required for perfusing the anatomical elements essential for maintaining the viability of foetuses at different gestational ages. 相似文献
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