Optimizing Koi Carp,Cyprinus carpio var. Koi (Linnaeus, 1758), Stocking Density and Nutrient Recycling With Spinach in an Aquaponic System     

T. Hussain  A. K. Verma  V. K. Tiwari  C. Prakash  G. Rathore  A. P. Shete  K. K. T. Nuwansi 《Journal of the World Aquaculture Society》2014,45(6):652-661

Fish waste water nutrient recycling in an aquaponic system was studied under different stocking densities of Koi Carp, Cyprinus carpio var. koi, along with spinach, Beta vulgaris var. bengalensis. Fish growth performance, plant growth, nutrient dynamics, and nutrient removal and their dependence on different stocking densities, namely 1.4, 2.1, and 2.8, were observed, of the different combinations, fish stocked at 1.4 kg/m³ had the best growth. Percent nutrient removal (NO₃–N, PO₄–P, and K) was significantly higher at 1.4 kg/m³. Thus, 1.4 kg/m³ stocking density can be suggested as optimum for Koi Carp production in spinach aquaponic systems.  相似文献   

Bovine brucellosis – a comprehensive review     

Sandip Kumar Khurana  Anju Sehrawat  Ruchi Tiwari  Minakshi Prasad  Baldev Gulati  Muhammad Zubair Shabbir  Rajesh Chhabra  Kumaragurubaran Karthik  Shailesh Kumar Patel  Mamta Pathak  Mohd. Iqbal Yatoo  Vivek Kumar Gupta  Kuldeep Dhama  Ranjit Sah  Wanpen Chaicumpa 《The Veterinary quarterly》2021,41(1):61

Brucellosis is a zoonotic disease of great animal welfare and economic implications worldwide known since ancient times. The emergence of brucellosis in new areas as well as transmission of brucellosis from wild and domestic animals is of great significance in terms of new epidemiological dimensions. Brucellosis poses a major public health threat by the consumption of non-pasteurized milk and milk products produced by unhygienic dairy farms in endemic areas. Regular and meticulous surveillance is essentially required to determine the true picture of brucellosis especially in areas with continuous high prevalence. Additionally, international migration of humans, animals and trade of animal products has created a challenge for disease spread and diagnosis in non-endemic areas. Isolation and identification remain the gold standard test, which requires expertise. The advancement in diagnostic strategies coupled with screening of newly introduced animals is warranted to control the disease. Of note, the diagnostic value of miRNAs for appropriate detection of B. abortus infection has been shown. The most widely used vaccine strains to protect against Brucella infection and related abortions in cattle are strain 19 and RB51. Moreover, it is very important to note that no vaccine, which is highly protective, safe and effective is available either for bovines or human beings. Research results encourage the use of bacteriophage lysates in treatment of bovine brucellosis. One Health approach can aid in control of this disease, both in animals and man.  相似文献   

AFLP Analysis of Genetic Diversity in Indian Soybean [Glycine max (L.) Merr.] Varieties     

C. Tara Satyavathi  K. V. Bhat  C. Bharadwaj  S. P. Tiwari  V. K. Chaudhury 《Genetic Resources and Crop Evolution》2006,53(5):1069-1079

AFLP technique was used to assess genetic diversity in 72 soybean varieties under cultivation in India. Selected 12 AFLP primer pairs produced 1319 products of which 1257 were polymorphic (95%). Wide variations were observed for the number of amplification products, percent polymorphism and average polymorphism information content (PIC). The Jaccard's similarity indices (J) based on the AFLP profiles of the 72 soybean varieties were subjected to UPGMA cluster analysis. The dendrogram generated revealed four major clusters, which were strongly supported by the high bootstrap values obtained from analyses of 1000 bootstrap samples. In addition, the Mantel's test for cophenetic correlation with r = 0.955 indicated very good fit of the varieties to a group in the cluster analysis. Some correspondence between the clustering pattern and the pedigree, place of release or target area of the variety was observed. Overall moderately high genetic diversity was observed which appears to be due to the higher genetic diversity prevalent in 12 of the varieties included in three diverse clusters and was indicative of the need to include more diverse germplasm in the soybean improvement programs.  相似文献   

Inheritance of neurotoxin (ODAP) content, flower and seed coat colour in grass pea (Lathyrus sativus L.)     

K. R. Tiwari  C. G. Campbell 《Euphytica》1996,91(2):195-203

Summary A strong epidemiological association is known to exist between the consumption of grass pea and lathyrism. A neurotoxin, -N-Oxalyl-L-, -diaminopropanoic acid (ODAP) has been identified as the causative principle. This study was undertaken to investigate the mode of inheritance of the neurotoxin ODAP, flower and seed coat colour in grass pea. Five grass pea lines with low to high ODAP concentration were inter-crossed in all possible combinations to study the inheritance of the neurotoxin. Parents, F₁ and F₂ progenies were evaluated under field condition and ODAP analyzed by an ortho-phthalaldehyde spectrophotometric method. Many of the progenies of low x low ODAP crosses were found to be low in ODAP concentration indicating the low ODAP lines shared some genes in common for seed ODAP content. The F₁ progenies of the low ODAP x high ODAP crosses were intermediate in ODAP concentration and the F₂ progenies segregated covering the entire parental range. This continuous variation, together with very close to normal distribution of the F₂ population both of low x low and low x high ODAP crosses indicated that ODAP content was quantitatively inherited. Reciprocal crosses, in some cases, produced different results indicating a maternal effect on ODAP concentration. Blue and white flower coloured lines of grass pea were inter-crossed to study the inheritance of flower colour. Blue flower colour was dominant over the white. The F₂ progenies segregated in a 13:3 ratio indicating involvement of two genes with inhibiting gene interactions. The gene symbol LB for blue flower colour and LW for white flower colour is proposed.  相似文献   

Molecular identification of an Ageratum enation virus isolate associated with mosaic disease of pointed gourd (Trichosanthes dioica) in India     

S. K. Raj  S. K. Snehi  M. S. Khan  A. K. Tiwari  G. P. Rao 《Phytoparasitica》2011,39(5):497-502

A severe mosaic disease of pointed gourd (Trichosanthes dioica Roxb.) was observed with significant disease incidence in Gopalganj, India, during 2008. Begomovirus was detected from symptomatic leaf samples by polymerase chain reaction (PCR) using coat protein gene-specific primers of a well characterized begomovirus which revealed positive amplification of expected size ~800 bp DNA band. To confirm begomovirus association, the complete DNA-A was amplified using three sets of begomovirus DNA-A primers. The amplicons were cloned, sequenced, and sequence of the complete DNA-A (2757 nt) was determined by combining the sequence data of all amplicons (Accession no. GQ268327). The sequence data showed 99–93% sequence identities and close phylogenetic relationships with isolates of Ageratum enation virus (AgEV). The begomovirus associated with mosaic disease of T. dioica was identified as an isolate of Ageratum enation virus, which is a new record from India.  相似文献   

Expression of δ-endotoxin Cry1EC from an inducible promoter confers insect protection in peanut (Arachis hypogaea L.) plants     

Tiwari S  Mishra DK  Chandrasekhar K  Singh PK  Tuli R 《Pest management science》2011,67(2):137-145

BACKGROUND: Spodoptera litura (F.) is a polyphagous foliage insect and a major pest on peanut (Arachis hypogaea L.). Constitutive expression of δ‐endotoxin Cry1EC gives protection against S. litura, as reported earlier. In this study, insect bites and salicylic acid induced high‐level expression of Cry1EC was achieved in peanut. In order to achieve this, the expression of pathogenesis responsive promoter PR‐1a was enhanced by placing it downstream of the CaMV35S promoter in the pCAMBIA 1300 backbone. The resultant promoter CaMV35S(r)PR‐1a expressed a high level of insecticidal δ‐endotoxin Cry1EC. The Gus expression under the control of CaMV35S(r)PR‐1a served as a convenient marker for evaluation of promoter response to different treatments. RESULTS: Transgenic events that showed a very low level of uninduced expression and no expression in seeds were selected. The Cry1EC expression in leaves increased nearly eightfold in the selected event, following induction by salicylic acid. Both the salicylic‐acid‐treated and the S. litura‐bitten leaves showed the highest expression after 2 days. Leaves from salicylic‐acid‐induced transgenic plants caused 100% mortality of S. litura at all stages of larval development. CONCLUSION: The results suggest that high expression of inducible promoters provides a good strategy for the development of safer transgenic food and feed crops. Copyright © 2010 Society of Chemical Industry  相似文献   

A note on distribution and potential of Japanese wild adzuki bean [Vigna angularis var. nipponensis (Ohwi) Ohwi and H. Ohashi] in India     

Bhatt  K. C.  Malav  Pavan Kumar  Gore  Padmavati G.  Tripathi  Kuldeep  Rathi  R. S.  Tiwari  Umeshkumar L.  Ahlawat  S. P. 《Genetic Resources and Crop Evolution》2021,68(5):2157-2166

Genetic Resources and Crop Evolution - The present paper deals with the report on the potential of Vigna angularis var. nipponensis (Ohwi) Ohwi and H. Ohashi assembled from Northeastern hill region...  相似文献   

Landraces-potential treasure for sustainable wheat improvement     

Adhikari  Sneha  Kumari  Jyoti  Jacob  Sherry Rachel  Prasad  Pramod  Gangwar  O. P.  Lata  Charu  Thakur  Rajnikant  Singh  Amit Kumar  Bansal  Ruchi  Kumar  Sundeep  Bhardwaj  S. C.  Kumar  Subodh 《Genetic Resources and Crop Evolution》2022,69(2):499-523

Genetic Resources and Crop Evolution - Agricultural production is facing serious threat from various biotic and abiotic stresses specifically under climatic challenges. It is becoming increasingly...  相似文献   

Microclimate modifications,growth and yield of intercrops under Hardwickia binata Roxb. based agroforestry system     

Arun K Shanker Scientist  Ram Newaj  P Rai  KR Solanki  K Kareemulla  R Tiwari 《Archives of Agronomy and Soil Science》2013,59(3):281-291

An experiment was conducted by planting Hardwickia binata Roxb. at 200, 400 and 800 trees ha^???1, intercrops viz., Brassica campestris (mustard) and Glycine max (soybean) were sown from the second year onwards in winter and summer respectively at the National Research Centre for Agroforestry, Jhansi, U.P., India with the objectives of evaluating agrosilvicultural potential of the system and to study the effect of tree densities on ecophysiology of understorey crops. Tree growth was affected by both density and intercrop in the initial years of growth. Photosynthetic photon flux density (PPFD) available to the intercrops reduced with increasing densities. Transpiration rate and stomatal conductance in intercrops decreased due to the presence of trees. No significant changes in leaf temperature were observed till the fifth year of the growing season. Yield was significantly higher in pure crop in comparison with all the densities in mustard. Soybean yield under 200 trees ha^???1 was comparable to that of the pure crop. Trees at the density of 200 trees ha^???1 provided a conducive microenvironment to the intercrops.  相似文献   

Institutional arrangement and typology of community forests of Meghalaya, Mizoram and Nagaland of North-East India   总被引：1，自引：0，他引：1  

B. K. Tiwari  H. Tynsong  M. M. Lynrah  E. Lapasam  S. Deb  D. Sharma 《林业研究》2013,24(1):179-186

Most community forests in hill regions of northeast India have been managed by traditional local institutions for centuries and most of these institutions remain functional even today. Higher forest coverage on private and community lands as compared to government land indicates that traditional institutions effectively manage community forests in the region. The present study was conducted through a survey of literature and field work using participatory research tools viz., PRA exercises, group discussions and questionnaire interviews with key informants in northeast India. We categorized the institutions involved in conservation and management of forests into three major types: traditional, quasi-traditional and modern. Traditional institutions with hierarchal structure were found in all states and are intact and functional in the state of Meghalaya. Quasi-traditional institutions, a blend of traditional and modern institutions were prevalent in Nagaland while modern institutions have almost replaced traditional institutions in Mizoram. We recorded at least eleven types of community forests viz., group of village forest, village forest, restricted forest, sacred forest, clan forest, cemetery forest, regeneration forest, bamboo forest, recreation forest, village reserved forest and medicinal plantation in villages of three states. The tribal people, through long-term trial and error experiments, have developed an elaborate, functional and generally democratic system of conservation and management of forests and associated natural ecosystems. Several forest and natural resource management lessons can be learnt from the institutional structure and decision making system of the evolving and dynamic institutions of tribal communities of the region.  相似文献