Infection with foot-and-mouth disease virus (FMDV) induces a natural killer (NK) cell response in cattle that is lacking following vaccination     

Jared R. Patch  Pervaiz A. Dar  Ryan Waters  Felix N. Toka  Jose Barrera  Christopher Schutta  Ganesh Kondabattula  William T. Golde 《Comparative immunology, microbiology and infectious diseases》2014

Natural killer (NK) cells play a role in innate antiviral immunity by directly lysing virus-infected cells and producing antiviral cytokines such as interferon gamma (IFN-γ). We developed a system for characterizing the bovine NK response to foot-and-mouth disease virus (FMDV), which causes a disease of cloven-hoofed animals and remains a threat to livestock industries throughout the world. IL-2 stimulation of PBMC resulted in poor killing of human K562 cells, which are often used as NK target cells, while lysis of the bovine BL3.1 cell line was readily detected. Depletion of NKp46-expressing cells revealed that 80% of the killing induced by IL-2 could be attributed to NKp46⁺ cells. In order to characterize the response of NK cells against FMDV in vivo, we infected groups of cattle with three different strains of the virus (A24 Cruzeiro, O1 Manisa, O Hong Kong) and evaluated the cytolytic ability of NK cells through the course of infection. We consistently observed a transient increase in cytolysis, although there was variation in magnitude and kinetics. This increase in cytolysis remained when CD3⁺ cells were removed from the preparation of lymphocytes, indicating that cytolysis was independent of MHC-T cell receptor interaction or γδ T cell activation. In contrast, animals monitored following vaccination against FMDV did not exhibit any increase in NK killing. These data suggest that NK cells play a role in the host immune response of cattle against FMDV, and contrast with the suppression of NK activity previously observed in swine infected with FMDV.  相似文献   

Stereotactic Radiosurgery and Fracture Fixation in 6 Dogs With Appendicular Osteosarcoma     

Jennifer L. Covey DVM  Diplomate ACVS  James P. Farese DVM  Diplomate ACVS  Nicholas J. Bacon Vet MB  Diplomate ACVS & ECVS  Pierre Amsellem DVM  Diplomate ACVS  Ryan P. Cavanaugh DVM  Diplomate ACVS  Rowan J. Milner BVSc  MMed Vet  Diplomate ACVIM 《Veterinary surgery : VS》2014,43(2):174-181

  相似文献   

Putative human and avian risk factors for avian influenza virus infections in backyard poultry in Egypt     

Basma M. Sheta  Trevon L. Fuller  Brenda Larison  Kevin Y. Njabo  Ahmed Samy Ahmed  Ryan Harrigan  Anthony Chasar  Soad Abdel Aziz  Abdel-Aziz A. Khidr  Mohamed M. Elbokl  Lotfy Z. Habbak  Thomas B. Smith 《Veterinary microbiology》2014

Highly pathogenic influenza A virus subtype H5N1 causes significant poultry mortality in the six countries where it is endemic and can also infect humans. Egypt has reported the third highest number of poultry outbreaks (n = 1084) globally. The objective of this cross-sectional study was to identify putative risk factors for H5N1 infections in backyard poultry in 16 villages in Damietta, El Gharbia, Fayoum, and Menofia governorates from 2010–2012. Cloacal and tracheal swabs and serum samples from domestic (n = 1242) and wild birds (n = 807) were tested for H5N1 via RT-PCR and hemagglutination inhibition, respectively. We measured poultry rearing practices with questionnaires (n = 306 households) and contact rates among domestic and wild bird species with scan sampling. Domestic birds (chickens, ducks, and geese, n = 51) in three governorates tested positive for H5N1 by PCR or serology. A regression model identified a significant correlation between H5N1 in poultry and the practice of disposing of dead poultry and poultry feces in the garbage (F = 15.7, p < 0.0001). In addition, contact between domestic and wild birds was more frequent in villages where we detected H5N1 in backyard flocks (F = 29.5, p < 0.0001).  相似文献   

Comparison of equine endometrial cytology samples collected with uterine swab,uterine brush,and low‐volume lavage from healthy mares     

Andrea A. Bohn  Ryan A. Ferris  Patrick M. McCue 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2014,43(4):594-600

  相似文献   

Treatment of Intraoperative Persistent Penile Erection in a Stallion     

Marlis L. Rezende  Ryan A. Ferris  Britta S. Leise  Khursheed R. Mama  David A. Scofield  Patrick M. McCue 《Journal of Equine Veterinary Science》2014

This case report describes the occurrence of persistent penile erection in a breeding stallion that occurred while the horse was under inhalant anesthesia for a carpal arthroscopy. The horse had no history of breeding problems, and no abnormalities were detected on physical examination, complete blood count, or serum chemistry tests performed prior to surgery. Anesthesia was induced with guaifenesin and ketamine after sedation with xylazine and was maintained with isoflurane in 100% oxygen. Penile erection developed approximately 35 minutes after induction and persisted for over 2 hours despite various physical and pharmacological attempts to alleviate it (massage, cold compresses, intravenous benztropine administration, and intracavernosal phenylephrine). Successful resolution of the erection was obtained by cannulation and drainage of blood from the corpus cavernosum and subsequent irrigation with heparinized sterile saline and infusion of phenylephrine in the corpus cavernosum. The detumescent penis was placed back into the sheath, and purse string sutures were placed in the sheath to ensure the penis would remain inside the sheath during recovery. The stallion's recovery from anesthesia was uneventful, the sutures were removed, and the horse was fitted with a penile sling to prevent additional edema or trauma. The stallion recovered completely from the persistent penile erection. Semen was collected 6 days after the event, and he returned to normal pasture breeding 6 weeks after surgery.  相似文献   

Environmental Manipulation of Growth and Sexual Maturation in Yellow Perch, Perca flavescens     

Laura N.  Shewmon  John R.  Godwin  Ryan S.  Murashige  Harry V  . Daniels Thomas M.  Losordo 《Journal of the World Aquaculture Society》2007,38(3):383-394

Yellow perch, Perca flavescens, exhibit sexually dimorphic growth detected as the fish enter puberty. The purpose of this study was to evaluate the differential influences of photoperiod and temperature on the sexual maturation and dimorphic growth pattern of yellow perch. Four combinations of constant (CP) or natural (NP) photoperiod with constant (CT) or natural (NT) temperature were evaluated (CPCT, CPNT, NPCT, NPNT). Following 6 months of growth, results indicate that (1) CT is the strongest promoter of overall growth regardless of photoperiod. Perch reared under CT were 26.4 ± 8.1 g heavier than perch reared under NT (P < 0.0001). (2) CP also promotes overall growth regardless of temperature. CP perch outgrew NP perch by 7.0 ± 8.1 g (P < 0.0872). (3) NP (decreasing) initiates maturation in yellow perch regardless of temperature. (4) NT is required for late maturational processes. While both NP groups initiated maturation, only NPNT females were able to recruit oocytes to a vitellogenic stage. Only NPNT males were able to sustain spermiation to the end of the study. (5) CPCT confer the best overall growth performance, fully inhibit maturation in both genders, and suppress a sexually dimorphic growth pattern up to 53 g average weight. These results indicate that using a CPCT regime during intensive fingerling production will produce a larger, more uniform fish population that could alleviate losses as a result of cannibalism. Perch that remained immature did not exhibit a dimorphic growth pattern.  相似文献   

Machine learning highlights the importance of primary and secondary production in determining habitat for marine fish and macroinvertebrates     

Kevin D. Friedland  Michelle Bachman  Andrew Davies  Romain Frelat  M. Conor McManus  Ryan Morse  Bradley A. Pickens  Szymon Smoliński  Kisei Tanaka 《水产资源保护:海洋与淡水生态系统》2021,31(6):1482-1498

1. Species distribution models for marine organisms are increasingly used for a range of applications, including spatial planning, conservation, and fisheries management. These models have been constructed using a variety of mathematical forms and drawing on both physical and biological independent variables; however, what might be called first-generation models have mainly followed the form of linear models, or smoothing splines, informed by data collected in the context of fish surveys.
2. The performance of different classes of variables were tested in a series of species occurrence models built with machine learning methods, specifically evaluating the potential contribution of lower trophic level data. Random forest models were fitted based on the classification of the absence/presence for fish and macroinvertebrates surveyed on the US Northeast Continental Shelf.
3. The potential variables included physical, primary production, secondary production, and terrain variables. For accepted model fits, six variable importance measures were computed, which collectively showed that physical and secondary production variables make the greatest contribution across all models. In contrast, terrain variables made the least contribution to these models.
4. Multivariable analyses that account for all performance measures reinforce the role of water depth and temperature in defining species presence and absence; however, chlorophyll concentration and some specific zooplankton taxa, such as Metridia lucens and Paracalanus parvus, also make important contributions with strong seasonal variations.
5. Our results suggest that lower trophic level variables, if available, are valuable in the creation of species distribution models for marine organisms.

  相似文献   

Evaluating changes in switchgrass physiology,biomass, and light-use efficiency under artificial shade to estimate yields if intercropped with Pinus taeda L.     

Janine M. Albaugh  Timothy J. Albaugh  Ryan R. Heiderman  Zakiya Leggett  Jose L. Stape  Kyle King  Katherine P. O’Neill  John S. King 《Agroforestry Systems》2014,88(3):489-503

There is growing interest in using switchgrass (Panicum virgatum L.) as a biofuel intercrop in forestry systems. However, there are limited data on the longevity of intercropped bioenergy crops, particularly with respect to light availability as the overstory tree canopy matures. Therefore, we conducted a greenhouse study to determine the effects of shading on switchgrass growth. Four treatments, each with different photosynthetically active radiation (PAR) levels, were investigated inside the greenhouse: control (no shade cloth, 49 % of full sunlight), low (under 36 % shade cloth), medium (under 52 % shade cloth), and heavy shade (under 78 % shade cloth). We determined the effect of shading from March to October 2011 on individually potted, multi-tillered switchgrass transplants cut to a stubble height of 10 cm. In the greenhouse, there was a reduction in tiller number, tiller height, gas exchange rates (photosynthesis and stomatal conductance), leaf area, above- and belowground biomass and light-use efficiency with increasing shade. Total (above- and belowground) biomass in the control measured 374 ± 22 compared to 9 ± 2 g pot^?1 under heavy shade (11 % of full sunlight). Corresponding light-use efficiencies were 3.7 ± 0.2 and 1.4 ± 0.2 g MJ^?1, respectively. We also compared PAR levels and associated aboveground switchgrass biomass from inside the greenhouse to PAR levels in the inter-row regions of a range of loblolly pine (Pinus taeda L.) stands from across the southeastern United States (U.S.) to estimate when light may limit the growth of intercropped species under field conditions. Results from the light environment of loblolly pine plantations in the field suggest that switchgrass biomass will be significantly reduced at a loblolly pine leaf area index between 1.95 and 2.25, which occurs on average between ages 6 and 8 years across the U.S. Southeast in intensively managed pine plantations. These leaf area indices correspond to a 60–65 % reduction in PAR from open sky.  相似文献   

Long-term catch trends and risk assessment of the Critically Endangered white-spotted wedgefish (Rhynchobatus djiddensis) from South Africa     

Ryan Daly  Denham Parker  Geremy Cliff  Gareth L. Jordaan  Nkabi Nomfundo  Rhett H. Bennett  Bruce Q. Mann 《水产资源保护:海洋与淡水生态系统》2021,31(4):777-788

1. The white-spotted wedgefish (Rhynchobatus djiddensis) is a Critically Endangered shark-like ray in the family Rhinidae. Throughout its Western Indian Ocean distribution, it is targeted for its valuable meat and fins and is reported to have undergone major population declines. However, there remains a need for species specific time-series data to accurately assess localized population declines.
2. This study used two independent long-term (37 and 40 years) time-series catch data from competitive shore angling and shark nets to investigate the size composition and catch per unit effort (CPUE) and conduct a risk assessment for the population on the east coast of South Africa.
3. From 1977 to 2017 the competitive shore fishery captured 7,703 individual R. djiddensis, whilst shark nets in the same region captured 2,856 individuals from 1981 to 2017. Individuals captured in the nets had a sex ratio of 1.8:1 females to males, and were larger than those caught by the anglers. Although the mean annual sizes of net-caught individuals were above the size of reported sexual maturity, there was little evidence to suggest that any individuals captured were reproductively active.
4. Both the competitive shore fishery and shark net catches exhibited strong seasonal trends with the majority of R. djiddensis catches occurring from October to May peaking in austral summer. Standardized CPUE from the competitive shore fishery declined substantially between 1977 and 2017 and shark net catches exhibited a significant (p < 0.05) fourfold decline in annual nominal CPUE from 1981 to 2017.
5. Ultimately, a risk assessment showed a 65.1% decline in abundance over a period of three generation lengths, which indicates that the sampled population of R. djiddensis in South Africa should be classified as Endangered according to the IUCN Red List using criterion A2b. The conservation implications of this are discussed.

  相似文献   

Selection for cold chipping genotypes from three early generations in a potato breeding program   总被引：1，自引：0，他引：1  

Ryan J. Hayes  Christian A. Thill 《Euphytica》2002,128(3):353-362

Chipping potatoes are an important market for the processing industry. Of the traits influencing chip quality,chip color is market limiting since other traits have diminished value without light chip color. Cold (4 ^°C) storage can reduce storage losses and cultivars producing light colored chips directly from cold storage are needed for this reason. Selection for cold chipping cultivars is traditionally practiced by visual evaluation of progeny the first few years in the field, followed by selection for cold chipping in years 4–5. Early generation selection for cold chipping may reduce cultivar development time by identifying good chipping progeny, families and parents earlier in the breeding cycle. Progeny from 175 4x × 4x families were evaluated from three early generations. They were greenhouse grown tubers (GGT) evaluated after6 mo. storage using randomly selected progeny, and field grown single hills propagated from either seedling transplants (FTR), or from greenhouse grown tubers (FGT). Progeny from field grown generations were selected first by visual evaluation, and then by random selection and chipped after6 mo. (visual selections) or 3 and 6 mo. storage (random selections). Cold chipping progenies were found in all three early generations. The earliest generations for selection are GGT or FTR, but may be less efficient than FGT. Correlations suggest that reliable parent and family selection is possible using GGT data. Visual selection eliminated 4/5 of the good chipping progeny, while identifying 8 of 11,714 genotypes combining visual merit and cold chipping potential. Increased genetic variation and sexual polyploidization are possible strategies for increasing the number of desirable genotypes. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献