An investigation of antibody response in cattle vaccinated with the rabbit-passaged LA rinderpest virus in Korea.     

J NAKAMURA  S KISHI  J KIUCHI  R REISINGER 《American journal of veterinary research》1955,16(58):71-75

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Experimental production of porcine gastroesophageal ulcers by betazole and reserpine     

EI-ICHI KOKUE  TAKASHI NAKAMURA  TOYOAKI HAYAMA 《Journal of veterinary pharmacology and therapeutics》1978,1(3):217-224

The ulcerogenic and secretogenic properties of betazole, (an H₂ agonist), and/or reserpine on pig stomachs were studied. (1) Betazole (50 mg/body) and reserpine (0.02 mg/kg) were successively injected (i.m.) for 5–10 days into starved animals. Gastroesophageal ulcers, pathologically similar to naturally occurring ulcers were observed in all pigs (10/10), whether a nutrient solution (5% glucose, 1 litre/day) was supplied or not. In the starved pigs given either betazole or reserpine, only a small ulcer or erosion was observed. (2) In four pigs which were given commercial mash at a rate of 3% of body weight per day and killed on the 6th or 10th day of successive injections of both drugs, the incidence of ulcers was 2/4. (3) The gastric secretogenic effect of betazole and/or reserpine was confirmed in pigs with Heidenhain gastric pouches. (4) From these data, a new method for the experimental induction of pig gastroesophageal ulcers, by means of simultaneous injections of betazole and reserpine for 5 days under the nutrient (glucose solution) drinking condition, is proposed. The authors consider that this method may be superior to the repository histamine method, with respect to the absence of adverse effects, practical convenience and pathological and etiological similarities with naturally occurring ulcers.  相似文献   

Functional indispensability of fibronectin associated on the mesenchymal cell surface during the early period of feather development in the chick embryo in vitro     

Ken KOBAYASHI  Shigeharu FUKUNAGA  Kazuaki TAKENOUCHI  Fumio NAKAMURA 《Animal Science Journal》2004,75(5):441-449

To clarify the role of fibronectin (FN) during the early period of feather development, reconstituted skin consisting of intact epithelium and isolated mesenchymal cells from embryonal chick skin was used. In early feather development, FN was localized around mesenchymal cells of the dermal condensation. Isolated mesenchymal cells had associated with FN on their surfaces. FN on the cell surface dissociated following EDTA treatment, and EDTA‐treated cells re‐associated with exogenous FN. The intact epithelium also bound to exogenous FN at the placode. When FN‐associated or FN‐reassociated mesenchymal cells were used, the reconstituted skin formed feather rudiments only at the positions where the epithelial placode existed originally, and the locality of tenascin transferred from the placode to the mesenchyme during the period of feather bud formation. However, in reconstituted skin using FN‐dissociated mesenchymal cells, feather rudiments did not form. Additionally, the epithelial placodes disappeared, and tenascin was distributed uniformly on the surface of the epithelium and not localized in the mesenchyme. These findings suggest that FN associated on the surfaces of mesenchymal cells maintains the functions of mesenchymal cells as dermal condensation and mediates epithelial‐mesenchymal interactions during the early period of feather development. The results also suggest that reconstituted skin is a useful tool for functional studies on the extracellular matrix.  相似文献   

Comparison of heat production and plasma lipid metabolites between meat- and egg-types of Nagoya breed chicken during embryonic development     

Momoka SATO  Kenji NODA  Katsutoshi KINO  Akihiro NAKAMURA  Mitsuhiro FURUSE 《Animal Science Journal》2007,78(6):613-618

We compared heat production (HP) and plasma lipid metabolites between meat‐ and egg‐types of Nagoya breed chickens during embryonic development. To investigate HP and respiratory quotient, oxygen consumption and carbon dioxide production were measured using an open‐circuit calorimeter system. HP was significantly lower in meat‐ than in egg‐type chickens during embryonic development, and HP gradually decreased with developmental stage in both types. The respiratory quotient was constant at approximately 0.68 at every embryonic stage investigated, and the value was similar in both types. Plasma concentrations of triacylglycerol, nonesterified fatty acid, glycerol and D‐3‐hydroxybutyrate, which are associated with lipid metabolism, were similar in both types during embryonic development. These results demonstrate that chicken embryos selected for rapid growth of Nagoya breed have characteristic lower HP, and that when selecting chickens for rapid growth, HP is an important parameter during embryonic stages.  相似文献   

Immunohistochemistry and molecular epidemiology of avian paramyxovirus 1 from formalin-fixed and paraffin-embedded sections of Japanese doves (Columba livia) affected with neurological signs     

Kikuyasu NAKAMURA  Hideo FUJIMORI  Akiko KOYAMA  Trinh Quang DAI  Kunitoshi IMAI  Mitsutaka IKEZAWA  Yu YAMAMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):837-841

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Analysis of a pair of END+ and END? viruses derived from the same bovine viral diarrhea virus stock reveals the amino acid determinants in Npro responsible for inhibition of type I interferon production     

Takashi KOZASA  Yuri ABE  Kazuya MITSUHASHI  Tomokazu TAMURA  Hiroshi AOKI  Masatoshi ISHIMARU  Shigeyuki NAKAMURA  Masatoshi OKAMATSU  Hiroshi KIDA  Yoshihiro SAKODA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(5):511-518

The Exaltation of Newcastle disease virus (END) phenomenon is induced by the inhibition of type I interferon in pestivirus-infected cells in vitro, via proteasomal degradation of cellular interferon regulatory factor (IRF)-3 with the property of the viral autoprotease protein N^pro. Reportedly, the amino acid residues in the zinc-binding TRASH motif of N^pro determine the difference in characteristics between END-phenomenon-positive (END⁺) and END-phenomenon-negative (END⁻) classical swine fever viruses (CSFVs). However, the basic mechanism underlying this function in bovine viral diarrhea virus (BVDV) has not been elucidated from the genomic differences between END⁺ and END⁻ viruses using reverse genetics till date. In the present study, comparison of complete genome sequences of a pair of END⁺ and END⁻ viruses isolated from the same virus stock revealed that there were only four amino acid substitutions (D136G, I2623V, D3148G and D3502Y) between two viruses. Based on these differences, viruses with and without mutations at these positions were generated using reverse genetics. The END assay, measurements of induced type I interferon and IRF-3 detection in cells infected with these viruses revealed that the aspartic acid at position 136 in the zinc-binding TRASH motif of N^pro was required to inhibit the production of type I interferon via the degradation of cellular IRF-3, consistently with CSFV.  相似文献   

Reemergence of Ibaraki disease in southern Japan in 2013     

Yoshimasa HIRASHIMA  Tomoko KATO  Makoto YAMAKAWA  Hiroaki SHIRAFUJI  Ryoichi OKANO  Tohru YANASE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1253-1259

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Analysis of the equine ovarian structure during the first twelve months of life by three-dimensional internal structure microscopy     

Mamiko ONO  Hiroki AKUZAWA  Yasuo NAMBO  Yuuko HIRANO  Junpei KIMURA  Satoko TAKEMOTO  Sakiko NAKAMURA  Hideo YOKOTA  Ryutaro HIMENO  Tohru HIGUCHI  Tadatoshi OHTAKI  Shigehisa TSUMAGARI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1599-1603

A three-dimensional internal structure microscopy (3D-ISM) can clarify the anatomical arrangement of internal structures of equine ovaries. In this study, morphological changes of the equine ovary over the first 12 months of life were investigated by 3D-ISM in 59 fillies and by histological analysis in 2 fillies. The weight and volume of the paired ovaries initially decreased from 0 to 1 months to 2 to 3 months of age and then significantly increased at 8 to 12 months of age. The ovulation fossa was first observed around the 3rd month and became evident after the 6th month. The number of follicles with a diameter of ≥10 mm and the diameter of the largest follicle increased gradually after 6 months of age. On a volume basis, the medulla accounted for nearly 90% of the whole ovary at 0 to 1 months of age, but significantly decreased from 2 to 3 months of age. The volume of the cortex increased progressively after birth and reached approximately 60% of the total volume at 8 to 12 months of age. This significant development of the cortex coincided with the increased number and size of large follicles observed from 6 months of age. These results suggest that the development of the cortex plays a role in the maturation of the follicles and the equine ovary undergoes substantial morphological changes postnatally until puberty.  相似文献   

A study of in vivo antihypertensive properties of enzymatic hydrolysate from chicken leg bone protein   总被引：1，自引：1，他引：0  

Fu-Yuan CHENG  Tien-Chun WAN  Yu-Tse LIU  Kung-Ming LAI  Liang-Chuan LIN  Ryoichi SAKATA 《Animal Science Journal》2008,79(5):614-619

Numerous attempts have been made to develop angiotensin I converting enzyme (ACE) inhibitors from various sources of food protein. Generally chicken leg bones are discarded after industrial chicken meat processing without any substantial benefit. In previous studies, chicken leg bone proteins were hydrolyzed by various enzymes and the results demonstrated that Alcalase hydrolysates have considerable ACE inhibiting activities. In this study, the best ACE inhibitory hydrolysate (A4) (which was derived from chicken leg bone protein by Alcalase after 4 h incubation) was orally administrated (50 mg/kg bw) in spontaneously hypertensive rats (SHRs) to investigate its antihypertensive effects. After oral administration of A4, a maximal reduction activity of about 26 mmHg was found at 4 h and maintained to 8 h. Moreover, SHRs treated with A4 (50 mg/kg bw/day) for eight weeks exhibited a reduction in systolic blood pressure, which is as significant as the effects of Captopril ( P  < 0.05). These results suggested that chicken leg bones have a high potential for utilization to develop ACE inhibitors as potential food ingredients intended to alleviate hypertension.  相似文献   

A method for extracting carboxy‐myoglobin from beef          下载免费PDF全文

Masahiro Waga  Shiro Takeda  Ryoichi Sakata 《Animal Science Journal》2018,89(2):467-473

The present study was conducted to devise a method for the effective extraction of carboxy‐myoglobin (COMb) from beef without carbon monoxide dissociation. The ratio of COMb to myoglobin was computed at absorptions of wavelengths 541 and 551 nm, which characterize COMb and the isosbestic point between COMb and deoxy‐myoglobin, respectively. The COMb extraction rate was found to vary with temperature, pH and oxygen conditions. The decrease observed in this rate was inversely proportional to the rise in extraction temperature. The COMb extraction rate was also affected by pH, and the stability of COMb in the extract solution was the highest at pH 8.0–9.0. Moreover, the presence of oxygen was found to disturb COMb extraction. According to these results, nearly all COMb could be extracted from carbon‐monoxide‐treated beef under stirring conditions in pH 8.5 deoxidized buffer, at 1°C, and under N₂ flow with the improved extraction method in this study (98.1 ± 2.7%). The decrement of COMb in the extract was accelerated by light, and the COMb was stable for 20 min in the dark, at 1°C. The extraction conditions for COMb described above should allow the accurate evaluation of COMb in meat tissue.  相似文献