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21.
Seki Y Seimiya YM Yaegashi G Kumagai S Sentsui H Nishimori T Ishihara R 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(12):1503-1508
During the period from 2001 to the following year, progenital diseases had been epidemic among the draft stallions and mares pastured together in Iwate Prefecture, the northeastern district of Japan. A stallion and 8 of 31 mares were affected in 2001, and 1 of 2 stallions and 10 of 36 mares in 2002. The clinical symptoms consisted of the formation of papules, pustules, ulcers and scabs on the progenital skin and mucosa in stallions and mares. In 2002, Equine herpesvirus 3 (EHV3) was isolated from 2 mares and the glycoprotein G gene of the virus detected from a stallion and 4 mares by polymerase chain reaction. Serum neutralizing tests showed that 12 of 38 horses, 10 clinically and 2 subclinically affected, changed to be positive for the EHV3 antibody. The results suggest that the horses were affected with equine coital exanthema (ECE) through coitus. Five mares with the antibody at the pre-pastured period may have been the possible origins of EHV3 infection in 2002, although the exact origin in 2001 remains unknown. The artificial insemination was performed for the prevention of ECE spreading through coitus on the pasture in 2003. There was no epidemic of the disease in 31 mares, although 3 mares with the antibody at the pre-pastured period showed the significant increase in the titers during the pastured period. 相似文献
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Aritada YOSHIMURA Takahiro OHMORI Kokoro ITOU Ryo ISHI Yuri MATSUMURA Yuhei WADA Miori KISHIMOTO Tomoko IWANAGA Naoki MIURA Kazuhiko SUZUKI Ryuji FUKUSHIMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(5):780
In dogs, pancreatic acinar cell injury is thought to be caused by decreased pancreatic blood flow due to heart failure. In previous our report, it demonstrated that decreased heart function causes a significant decrease in pancreatic blood flow in heart failure dog model caused by rapid ventricular pacing (RVP). However, the types of histopathological changes remain unclear. We aimed to verify the types of histopathological changes occurring in the pancreatic tissue due to decreased heart function. After RVP for 4 weeks, atrophy of pancreatic acinar cells, characterized by a decrease in zymogen granules, was observed in all areas of the pancreas. In conclusion, the result of this study suggests that attention should be paid to ischemia/hypoperfusion injury in the pancreas. 相似文献
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Oue Y Ishihara R Edamatsu H Morita Y Yoshida M Yoshima M Hatama S Murakami K Kanno T 《Veterinary microbiology》2011,150(1-2):41-48
A new equine coronavirus was isolated from the feces of adult horses with pyrogenic and enteric disease. The disease outbreak was mainly observed among 2- to 4-year-old horses living in stables of a draft-horse racetrack in Japan. On comparing the isolated virus (isolate Tokachi09) with the equine coronavirus NC99 strain, no significant differences were observed in several biological properties such as hemagglutinating activity, antigenicity (in indirect immunofluorescence and neutralization tests), and one-step growth (in cell culture). The sequences of the nucleocapsid and spike genes of isolate Tokachi09 showed identical size (1341 and 4092 nucleotides, 446 and 1363 amino acids, respectively) and high similarity (98.0% and 99.0% at the nucleotides, 97.3% and 99.0% at the amino acids, respectively) to those of strain NC99. However, the isolate had a 185-nucleotide deletion from four bases after the 3'-terminal end of the spike gene, resulting in the absence of the open reading frame predicted to encode a 4.7-kDa nonstructural protein in strain NC99. These results suggest that the 4.7-kDa nonstructural protein is not essential for viral replication, at least in cell culture, and that the Japanese strain probably originated from a different lineage to the North American strain. This is the first equine coronavirus to be isolated from adult horses with pyrogenic and enteric disease. 相似文献
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Sanae FUKUSHIMA Kimie NIIMI Eiki TAKAHASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(2):161-166
The classic piebald mutation in the endothelin receptor type B (Ednrb) gene was found on rolling Nagoya genetic background (PROD-s/s) mice with white coat spotting. To examine whether genetic background influenced the phenotype in the piebald mutant mice, we generated a congenic strain (B6.PROD-s/s), produced by repeated backcrosses to the C57BL/6J (B6) strain. Although B6.PROD-s/s mice showed white coat spotting, 7% of B6.PROD-s/s mice died between 2 and 5 weeks after birth due to megacolon. The PROD-s/s, s/s and Japanese fancy mouse 1 (JF1) strains, which also have piebald mutations on different genetic backgrounds with B6, showed only pigmentation defects without megacolon. In expression analyses, rectums of B6.PROD-s/s
with megacolon mice showed ~5% of the level of Ednrb gene expression versus B6 mice. In histological analyses, aganglionosis was detected in the rectum of megacolon animals. The aganglionic rectum was thought to lead to severe constipation and intestinal blockage, resulting in megacolon. We also observed an abnormal intestinal flora, including a marked increase in Bacteroidaceae and Erysipelotrichaceae and a marked decrease in Lactobacillus and Clostridiales, likely inducing endotoxin production and a failure of the mucosal barrier system, leading ultimately to death. These results indicate that the genetic background plays a key role in the development of enteric ganglion neurons, controlled by the Ednrb gene, and that B6 has modifier gene (s) regarding aganglionosis. 相似文献
25.
Isogai A Utsunomiya H Kanda R Iwata H 《Journal of agricultural and food chemistry》2005,53(10):4118-4123
Changes in the aroma of sake during aging were investigated by aroma extract dilution analysis (AEDA) and quantitative analysis using the stir bar sorptive extraction method. In AEDA, more odor zones were detected in aged sake than in fresh sake. The dilution factors of aldehydes, polysulfides, and some esters were greater in the aged sake, and their increase during aging was confirmed through a quantitative analysis of sake stored for 0-35 years. Among these compounds, 3-methylbutanal, methional, and dimethyltrisulfide (DMTS) were present in aged sake at concentrations exceeding their odor thresholds, and the highest odor active value was observed for DMTS. Sensory tests showed that supplementation with DMTS contributed to both the total odor intensity and the sulfury odor of aged sake aroma. 相似文献
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粳稻食味品质改良研究现状与展望 总被引：5，自引：2，他引：3
进入21世纪,我国北方特别是东北粳稻面积不断扩大,单产明显提高,品质稳定改善,改良食味品质是今后重要研究内容。本文主要从食味品质鉴定方法,稻米淀粉种类和含量及分子结构、蛋白质种类和含量、其他物质含量与食味品质的关系,饭粒表面和内部超微结构与食味品质的关系,食味品质相关性状遗传分析与育种应用等方面介绍了日本粳稻食味品质研究现状,认为结合东北粳稻实际深入开展食味品质育种、栽培和生物技术等应用基础研究,充分利用籼稻二次枝梗粒偏向穗轴上部分布、穗颈大维管束数和一次枝梗数多等有利基因,同时减少对食味品质的不良影响,是今后北方尤其是东北籼粳稻杂交优质高产育种的重要研究课题。 相似文献
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根据已经获得的两种鲢肌球蛋白重链同工型基因(低温型sc-w和高温型sc-s)在3′端展现的明显差异,设计了2个特异性的反向引物,以鲤科鱼类肌球蛋白重链5′端的保守序列为正向引物,通过Long-PCR对编码鲢两种肌球蛋白重链同工型的球状结构域(Subfragment-1,S1)的全长基因进行了克隆和测序,并推断出它们一级结构的氨基酸序列。研究结果表明,sc-w与sc-s在S1的初级结构上显示80.5%的同源性、与已经报道的草鱼低温型(gc10)有97.2%的高同源性;sc-s则与草鱼中间型(gcI)和高温型(gc30)显示了分别为98.4%和97.1%的高同源性。低温型的sc-w和gc10在S1初级结构上展现的特有变异主要发生在43个氨基酸残基位点,其中15个属保守性残基。对S1区域中两个功能性的表面环loop1(与ATP结合位点有关)和loop2(与肌动蛋白结合位点有关)的结构解析发现,sc-w和gc10在两个表面环的长度、残基电荷分布和氨基酸组成等方面与其它同工型之间存在明显差异,揭示了这两个表面环的结构差异可能影响了栖息于不同环境温度下的淡水鱼的肌球蛋白分子马达功能。分子系统树的分析结果进一步证明,鱼类栖... 相似文献
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Uemura R Sueyoshi M Taura Y Nagatomo H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(8):899-903
Edema disease (ED) of pigs is an enterotoxaemic disease caused by enterotoxaemic Escherichia coli (ETEEC) infection. Antimicrobial therapy for pigs with ED is controversial because it may induce death of sickish piglets. In this study, we investigated the effects in vitro of 7 antimicrobial agents, ampicillin, gentamicin, colistin, bicozamycin, fosfomycin, sulfamethoxazole-trimethoprim and enrofloxacin, on the release and production of shiga toxin (Stx) 2e by ETEEC strains. We found that more Stx 2e accumulated in the bacterial cells than was released into supernatant. Associated with inhibition of cell wall synthesis, the exposure to ampicillin or fosfomycin increased the release of Stx 2e. The production levels of Stx 2e in all antimicrobial-treated cultures were equal to the level in the control or less than in the control. These results suggest that cell wall synthesis inhibitors, such as ampicillin and fosfomycin, may change for the worse in the signs in ETEEC infectious pigs. On the other hand, gentamicin, colistin, bicozamycin and enrofloxacin may be useful for the treatment of pigs with ED. 相似文献
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Ryoko Hirata Satoshi Ito Masatake G. Araki Yasushi Mitsuda Masahiro Takagi 《Journal of Forest Research》2014,19(6):514-522
The effects of late weeding treatment on the growth of young hinoki (Chamaecyparis obtusa) during the sixth growing season after planting were examined. Furthermore, the contribution toward hinoki growth recovery of crown productivity (NAR) and biomass allocation to crown (CAR) were determined. In the late weeding plot, no decline in height growth was observed subsequent to weeding, and growth in diameter at breast height (DBH) and crown projection area (CPA) began to recover subsequent to weeding; however, DBH and CPA were reduced and experienced a 1- to 1.5-year growth delay compared to values associated with the weeding plot at the end of the second year subsequent to weeding. Relative growth rate (RGR) and NAR in the late weeding plot recovered and possessed similar values to those of the weeding plot in the second year subsequent to weeding. CAR values of the late weeding plot were similar to those of the weeding plot both before and after weeding. These results suggested that the recovery of NAR rather than that of CAR was primarily responsible for the recovery of RGR. The present study demonstrated that hinoki were able to quickly acclimate to an environment dramatically altered by late weeding and recover growth rate within a short period of time. The present study also showed that delays in crown expansion associated with late weeding may have impeded subsequent matter production. Therefore, it was concluded that late weeding treatments should be employed only when the associated delays in growth are taken into account. 相似文献