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71.
汽车变速器的关键部件齿轮因其加工和装配质量的影响,工作时容易引起振动噪声大及承载能力降低等问题,而齿轮修形是解决这些问题的有效方法,且成本较低。针对某款汽车变速器,利用Romax对齿轮进行修形分析。结果表明,修形后减小了齿轮的传动误差和最大接触应力,齿面载荷分布更加合理。有效改善了齿轮的传动性能,增加了齿轮寿命。 相似文献
72.
The efficiency of nitrogen in cattle manures when applied to a double‐annual forage cropping system 下载免费PDF全文
B. Perramon À. D. Bosch‐Serra F. Domingo‐Olivé J. Boixadera 《Grass and Forage Science》2017,72(4):676-690
The use of cattle manure (CM) for fertilization presents challenges for optimizing nitrogen (N) use. Our work aimed to assess N efficiencies, in a 6‐year experiment with three biennial rotations of four crops: oat–sorghum (first year) and ryegrass–maize (second year) in a rainfed humid Mediterranean area of Spain. Fertilization treatments included the following: control (no N), 250 kg mineral N ha?1 year?1 (250MN), three CM rates (supplying 170, 250 and 500 kg N ha?1 year?1) and four treatments where the two lowest CM rates were complemented with either 80 or 160 kg mineral N ha?1 year?1. Treatments were distributed randomly in each of three blocks. Maximum dry‐matter yield (~44–49 t ha?1 rotation?1) was achieved in the third rotation, and only the control and the 170CM yielded significantly less. Within the limitations of the EU Nitrate Directive, the N steady state supply of 170CM always requires a complement of mineral N (80 kg N ha?1) to maximize N agronomic efficiency. The maximum N‐fertilizer replacement value (250CM vs. 250MN) was 0·67, without significant differences between the two treatments in other N‐related efficiency indexes, which indicates that plants took advantage of residual‐N effects. Nitrogen losses by leaching in the 250CM treatment were around 5–7% of the N applied. This reinforces the sustainability of manure recycling in long cropping seasons. 相似文献
73.
Silage and total mixed ration hygienic quality on commercial farms: implications for animal production 下载免费PDF全文
T. Cogan R. Hawkey E. Higgie M. R. F. Lee E. Mee D. Parfitt J. Raj S. Roderick N. Walker P. Ward J. M. Wilkinson 《Grass and Forage Science》2017,72(4):601-613
Implications of silage hygienic quality for animal production were investigated on forty‐five dairy farms in South West England. Samples of grass and maize silages and of total mixed rations (TMR) were obtained together with information on silage technology, herd size and animal production. Samples were analysed for mycotoxins, bacteria, yeasts, moulds and chemical composition. Thirteen mycotoxins were assayed, but none were detected in the samples of grass silage. However, mycotoxins were found in 0·9 of all maize and other silage samples, with deoxynivalenol and zearalenone predominating. There was no relationship between total mycotoxin concentration and mean lactation milk yield per cow. Enterobacteria counts tended to be higher in maize silage than in grass silage and higher still in TMR – a cause for concern. There were no relationships between mould counts and mycotoxin concentrations in silages, implying that mycotoxins may have been produced in the field pre‐ensiling. 相似文献
74.
尿苷二磷酸糖基转移酶(uridine diphosphate glycosyltransferases,UGTs)催化糖基转移反应,与植物次生代谢密切相关。本研究根据甜叶菊(Stevia rebaudiana)转录组数据库,克隆到一个催化莱鲍迪D苷(rebaudioside D,RD)合成的新型糖基转移酶候选基因,对其开展生物信息学分析。结果表明,该基因开放阅读框长1380 bp,编码459个氨基酸,等电点(pI)预测为5.54,理论分子量约49.66 kD,系统发育分析表明该基因与向日葵中的UGT89A2同源,故将其命名为SrUGT89A2。构建pET28a-SrUGT89A2原核表达载体,并在大肠杆菌(BL21(DE3))中诱导表达得到重组蛋白,HPLC检测表明粗酶液能催化甜叶菊提取液形成一个新的色谱峰,该峰保留时间与莱鲍迪D苷一致。经进一步纯化UGT89A2蛋白,添加不同甜菊糖苷标准品为催化底物,但未鉴定出该蛋白催化的具体糖苷。该潜在催化甜菊糖RD苷合成的新型糖基转移酶基因SrUGT89A2的发现,为RD苷的生物合成和甜菊糖苷的生物途径研究提供新的理论依据。 相似文献
75.
Frdrick G. Sunstrum Wubishet A. Bekele Charlene P. Wight Weikai Yan Yuanhong Chen Nicholas A. Tinker 《Plant Breeding》2019,138(1):82-94
We developed 178 recombinant inbred lines from a southern‐by‐spring oat population designated as “TxH.” These lines were genotyped to generate a high‐quality linkage map that resolved 6,902 markers into 21 linkage groups that matched closely with the latest hexaploid oat consensus map. Three major quantitative trait loci (QTLs) affecting heading date were found in locations that are consistent with known QTLs and candidate genes, and two other QTLs affecting heading date were found in novel locations. Five QTLs affecting plant height were found. Both sets of QTLs are responsible for transgressive segregation observed for these two traits. Four QTLs affecting resistance to crown rust, caused by the pathogen Puccinia coronata f. sp. avenae, were identified. Two of these QTLs are consistent with known clusters of rust resistance genes, while two may represent new locations of novel rust resistance genes. A complete set of SNP sequences suitable for generating markers for molecular selection is provided. 相似文献
76.
为评价氟铃脲在韭菜中使用的安全性,开展氟铃脲在韭菜中的残留量及残留降解研究。进行1年4地田间试验。消解动态试验按氟铃脲11250 g/ha(562.5 g a.i/ha,推荐最高剂量的1.5倍)施药;最终残留试验按氟铃脲11250 g/ha(562.5 g a.i/ha,推荐最高剂量的1.5倍)和7500 g/ha(375 g a.i/ha,推荐最高剂量)施药,施药1~2次,施药间隔7d,1次施药按照药后间隔10、14、21d采集韭菜样品;2次施药按照末次药后间隔7、10、14d采集韭菜样品。液相色谱串联质谱法对氟铃脲进行定量分析。田间消解动态试验表明:氟铃脲在韭菜中消解较快,在山东保护地和安徽露地半衰期分别为7.7和11.5d。膳食风险评估结果表明,氟铃脲在韭菜中的残留风险处于安全水平。建议使用5%氟铃脲乳油防治韭蛆,用药量7500~11250 g/ha,韭菜收割后2~3d,根部喷淋,最多施药2次,施药间隔7d,安全间隔期为14 d。 相似文献
77.
78.
Evaluating the Effects of Dietary Prebiotic Mixture of Mannan Oligosaccharide and Poly‐β‐Hydroxybutyrate on the Growth Performance,Immunity, and Survival of Rainbow Trout,Oncorhynchus mykiss (Walbaum 1792), Fingerlings 下载免费PDF全文
Ebrahim H. Najdegerami Amir Tokmachi Farideh Bakhshi 《Journal of the World Aquaculture Society》2017,48(3):415-425
This study investigated the effects of dietary poly‐β‐hydroxybutyrate (PHB) and mannan oligosaccharide (MOS) on the growth performance, digestive enzyme activities, and disease resistance of rainbow trout fingerlings. A total of 420 fingerlings, distributed equally into four different groups, each with three replicates, were fed a basal diet (control), 2% PHB (PHB), 0.2% MOS (MOS), or a diet supplemented with a mixture of 2% PHB and 0.2% MOS (PHB+MOS) for 60 d. Results showed that dietary PHB and/or MOS did not cause significant improvement in the growth performance of the fingerlings. The gut pH was markedly reduced; however, the activities of the digestive enzymes (except for pepsin and amylase by dietary PHB) were not significantly improved by the dietary supplements. Results also showed that dietary PHB and/or MOS markedly increased the resistance of the fingerlings toward Yersinia ruckeri infection, while the lowest total antibody was observed in fish fed control and MOS, indicating that MOS exerts its protective effects by modulating other mechanisms. Overall, this study provided a first screening effort to determine the effects of PHB and/or MOS as dietary supplements in rainbow trout fingerlings to develop an optimal prebiotic mix for improving the growth and health status of rainbow trout. 相似文献
79.
80.
Spatiotemporal patterns of mountain whitefish (Prosopium williamsoni) in response to a restoration of longitudinal connectivity 下载免费PDF全文
Peter M. Kiffney Ben Cram Paul L. Faulds Karl Burton Michele Koehler Thomas P. Quinn 《Ecology of Freshwater Fish》2018,27(4):1037-1053
To examine the role of longitudinal connectivity on the spatial and temporal dynamics of mountain whitefish (Prosopium williamsoni), we quantified movement and population dynamics following installation of the Landsburg Dam fishway, Cedar River, WA, USA. Mountain whitefish is widely distributed, poorly studied and not the focus of restoration. Before the fishway, mountain whitefish were not observed above the dam. Here, we focus on snorkel counts collected at reach and mesohabitat (e.g. pools) scales over 11 summers on the 20‐km above‐dam segment following restoration. A camera within the ladder provided number, size and movement timing, thereby informing on behaviour and recolonisation. Segment‐scale abundance increased following fish passage reaching an asymptote in 7 years, and mountain whitefish were detected throughout the main stem in 10 years. Annual movement through the ladder increased over time and was positively correlated with instream abundance and discharge, but negatively correlated with water temperature. About 60% of fish movements occurred in spring and early summer, potentially for foraging opportunities. Reach‐scale abundance peaked between 7 and 10 km from the dam; deep, cool (~10.6 to 11.6°C) conditions characterised these reaches. At the mesohabitat scale, mountain whitefish detection increased with depth and velocity after accounting for distance from the dam. Our results show how restoring longitudinal connectivity allowed this nontarget species to colonise newly available habitat. Their response supports the critical roles of longitudinal connectivity and environmental conditions, that manifest at different spatial scales, in dictating how freshwater fish respond to habitat disturbance. 相似文献