Genetic diversity patterns in ex situ collections of <Emphasis Type="Italic">Oryza officinalis</Emphasis> Wall. ex G. Watt revealed by morphological and microsatellite markers     

Maria Elizabeth Boncodin Naredo  Sheila Mae Quilloy Mercado  Maria Celeste Naynes Banaticla-Hilario  Myrricar Loren Berdos  Mario Alcantara Rodriguez  Kenneth Lee McNally  Ruaraidh Sackville Hamilton 《Genetic Resources and Crop Evolution》2017,64(4):733-744

This study was conducted to understand variation patterns and establish the population structure in the wild rice species, Oryza officinalis, using accessions collected across the 11 regions/areas of endemism in Asia and Oceania and conserved ex situ in the International Rice Genebank at the International Rice Research Institute. Morphological and microsatellite data detected a tendency for latitudinal divergence in O. officinalis and divided the accessions into (1) Malesian and (2) South and East Asian populations. Cluster and ordination analyses of morphological characters revealed that accessions from Malesia (Regions 1–6), Vietnam (Region 7), and Central Thailand (Region 8) showed short (16.19 ± 3.70 cm) and slender (1.53 ± 2.06 cm) flag leaves that are erect to almost horizontal, short panicles (25.19 ± 3.02 cm) and mainly purple internodes; and (3) accessions from Northern Thailand and Myanmar (Region 9), India (Region 10) and China (Region 11) characterized by long (29.57 ± 4.94 cm) and broad (2.18 ± 3.33 cm) flag leaves that are horizontal to drooping, long panicles (35.26 ± 2.63 cm) and green internodes. With 79.3 % of the SSR markers being polymorphic, molecular analyses detected an average of 5.31 alleles per marker with a total of 154 alleles. Differentiation was evident among the regions with FST = 0.2173, although there is an apparent loss of heterozygosity across regions indicated by an over-all positive FIS. Based on the results, issues related to the management of O. officinalis, specifically filling gaps in the collection and the formulation of seed multiplication practices are addressed.  相似文献   

Resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to spinetoram: inheritance and cross‐resistance to spinosad     

Ewerton C Lira  Anderson Bolzan  Antonio RB Nascimento  Fernando SA Amaral  Rubens H Kanno  Ingrid S Kaiser  Celso Omoto 《Pest management science》2020,76(8):2674-2680

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姬松茸子实体中活性多肽提取工艺初探   总被引：4，自引：0，他引：4  

洒威  焦迎春  郑晓冬 《青海农林科技》2005,(2):1-3

通过单因素考察及正交实验法对姬松茸子实体活性肽提取条件进行了优化。结果显示在姬松茸干子实体粉中加入8倍体积的水,在温度为4℃和pH9．0的条件下,用70％乙醇的沉淀提取6小时,得到提取液的活性肽的含量及抗氧化性均最优,经毛细管电泳图谱分析其活性肽分布也较适宜。  相似文献   

Progress on role of exosomes derived from leukemia cells     

ZHANG Juan-juan  LI Rui-wei  HE Ji-gang  OUYANG Hong-mei  SA Ya-lian 《园艺学报》2017,33(12):2287-2292

Exosomes are bilayer-lipid membrane nanovesicle from almost all living cell types which are involved in intercellular substance transporting and signaling communication. Exosomes are 30~120 nm in diameter, can transfer bioactive molecules including DNA, RNA, microRNA, protein as well as lipids derived from parents' cells to recipient cells by body fluids, and specifically influence their physiological or pathological conditions. Leukemia is due to malignant proliferation of hematopoietic stem and progenitor cells. It was reported that leukemia cells derived exosomes play a key role in disease progression, drug resistance, and predict prognosis. This paper will outline the role of exosomes derived from leukemia cells and provide important information to help explore the molecular pathogenesis, biomarker as well as therapeutic target of leukemia.  相似文献   

豆米轮作对春玉米土壤固氮细菌群落结构及多样性的影响     

提俊阳  张玉芹  杨恒山  张瑞富  邰继承  萨如拉  韩镁琪 《中国农学通报》2023,39(2):44-50

为探明大豆-玉米轮作对土壤固氮细菌的影响，以玉米连作为对照(MMM)，以nif-H基因为指示，采用高通量测序技术，分析大豆-玉米2年轮作周期(MSM)和大豆-玉米-玉米3年轮作周期(SMM)下土壤固氮细菌群落结构及多样性。结果表明：大豆-玉米轮作下0～20、20～40 cm土层土壤有机质、全氮、碱解氮、铵态氮和硝态氮均显著增加。固氮细菌的Chao1指数和ACE指数在0～20、20～40 cm土层均为轮作高于对照，轮作下MSM显著高于SMM;Shannon指数和Simpson指数在0～20 cm土层轮作显著高于MMM;20～40 cm土层MSM显著高于MMM。在门水平上，MSM和SMM的0～20、20～40 cm土层变形菌门的相对丰度均高于MMM。在属水平上，2个轮作类型0～20 cm土层的慢生根瘤菌属、弗兰克氏菌属和纤毛菌属相对丰度显著较高；20～40 cm土层慢生根瘤菌属、伪食酸菌属、地杆菌属和固氮弧菌属相对丰度显著较高；MSM 0～20、20～40 cm土层慢生根瘤菌属、甲基孢囊菌属和固氮弧菌属较SMM均提高。方差分析表明，固氮细菌丰度指数（Chao1和ACE指数）与有机质、碱解氮...  相似文献