A potentially useful distribution model for dietary intake data     

Myles JP  Price GM  Hunter N  Day M  Duffy SW 《Public health nutrition》2003,6(5):513-519

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Extraction methods for quantitation of gentamicin residues from tissues using fluorescence polarization immunoassay   总被引：1，自引：0，他引：1  

S A Brown  D R Newkirk  R P Hunter  G G Smith  K Sugimoto 《Journal of the Association of Official Analytical Chemists》1990,73(3):479-483

Sodium hydroxide digestion of unhomogenized kidney and skeletal muscle for 20 min at 70 degrees C was a superior method for extracting gentamicin from tissues, compared with simple homogenization, trichloroacetic acid precipitation of homogenized tissue, and sodium hydroxide digestion of homogenized tissue. Fluorescence polarization immunoassay was used to quantitate gentamicin. Sodium hydroxide digestion of unhomogenized tissue allowed for the recovery of 90.0 +/- 5.9% (means +/- SD) from renal cortex and 79.9 +/- 3.5% from skeletal muscle. The limit of sensitivity was 17.4 ng/g kidney tissue, 15.8 ng/g digested muscle, and 39.0 ng/g digested heart. The within-assay coefficient of variation (CV) at 100 ng/g kidney was 9.2%; at 500 ng/g kidney, the CV was 2.5%; and at 2000 ng/g kidney, the CV was 1.5%. The between-assay coefficient of variation was less than 7.5% for all concentrations from kidney, and the 99% confidence interval at 100 ng/g kidney was 71.7-112.4 ng gentamicin/g kidney. The within-assay coefficient of variation (CV) at 100 ng/g muscle was 15%; at 500 ng/g muscle, the CV was 2.6%; and at 2000 ng/g muscle, the CV was 2.3%. The between-assay coefficient of variation was less than 15% for all concentrations from muscle, and the 99% confidence interval at 100 ng/g muscle was 72.5-136.8 ng gentamicin/g muscle. Gentamicin-free milk could be distinguished from milk containing gentamicin concentrations of 10 ng/mL milk with 95% confidence, and from milk containing concentrations of 30 ng gentamicin/mL milk with 99% confidence. Quantitative results at or below the tolerance level can be obtained within 90 min of sample acquisition using these extraction and assay methods.  相似文献   

Residual effects of fungicidal spray programmes on apple trees     

Robert J. W. Byrde  Christopher W. Harper  Margaret E. Holgate  Thomas Hunter 《Pest management science》1973,4(3):327-333

Spraying programmes for apple scab and mildew control sometimes had residual effects for one or even two years. Thus, benomyl (0.025% a.i.) and pyridinitril (0.035% a.i.) drastically reduced the subsequent incidence of cankers caused by Nectria galligena on young wood. Apple scab fruit infection, caused by Venturia inaequalis, was also less on trees on which benomyl had been used in the previous season. In the bark of such trees, toxic residues equivalent to 1 to 4 μg benomyl/g fresh wt. were detected by bioassay 7 months after spraying.  相似文献   

University of Edinburgh veterinary expeditions: 1966 to 1986     

A G Hunter  J A Hammond 《The Veterinary record》1988,122(22):532-535

Since 1966, teams of new graduates from the Royal (Dick) School of Veterinary Studies have organised eight veterinary research expeditions to Africa, tropical America and the Seychelles. The expeditions are now a regular feature of the school's activities. It is now possible to look back, learn and advise on future expeditions regarding timing, research and funding.  相似文献   

Ovine internal parasitism in the Yemen Arab Republic     

A. G. Hunter  P. J. Heath 《Tropical animal health and production》1984,16(2):95-106

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Acute myopathy in horses at grass in east and south east Scotland     

B D Hosie  P W Gould  A R Hunter  J C Low  R Munro  H C Wilson 《The Veterinary record》1986,119(18):444-449

A myopathy of horses at grass in east and south east Scotland was recognised in the autumn and winter of 1984 and the spring of 1985. The clinical signs resembled those of paralytic myoglobinuria. Grossly increased creatine kinase activities and the passage of dark brown urine were consistent features. However, the horses were not in training, most of them died and the muscles affected were those of posture and respiration rather than movement. The condition may be unrelated to nutritional myopathy because all the cases had adequate levels of alpha-tocopherol although their selenium status varied from normal to deficient. The clinical and pathological findings in 12 cases are presented and the differential diagnosis and possible aetiologies discussed.  相似文献   

Prediction of herbage intake and liveweight gain of cattle grazing tropical pastures from the composition of the diet     

B.D. Siebert  R.A. Hunter 《Agricultural Systems》1977,2(3):199-208

As a basis for calculating growth rates of cattle within a model of a beef herd, three experiments were carried out. Their purpose was to (i) develop equations to predict organic matter, digestible organic matter and metabolisable energy intakes of cattle, (ii) establish the conversion of metabolisable energy to live weight of tropical cattle, and (iii) establish the validity of these relationships for predicting the liveweight change of grazing animals.In the first experiment, Brahman × Shorthorn crossbred cattle were held either indoors in pens or in long laneways outdoors where they walked approximately 5 km per day. Organic matter intake was measured in both situations and was found to be not significantly different between the situations. Regression equations were calculated relating intake of organic matter and digestible organic matter by cattle indoors to the chemical composition of their diet. The conversion of digestible organic matter to metabolisable energy intake and finally to liveweight gain was confirmed in a second experiment. The liveweight gain of outdoor animals was predicted accurately provided the metabolism of crossbred animals was considered and an allowance made for the energy required for walking.The diet selected by oesophageally fistulated animals at pasture was chemically analysed over a four-month period following these measurements. Feed intake and liveweight gain of non-fistulated animals grazing native and improved pastures were predicted from the chemical composition of the diet selected. There was good prediction of liveweight gain in both pasture situations.  相似文献   

Diseases of cattle—A manual of diagnosis     

A. G. Hunter 《Tropical animal health and production》1992,24(2):120-120

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A. G. Hunter 《Tropical animal health and production》1992,24(2):120

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Observations on blood coagulation after snakebite in dogs and cats     

SA. HOLLOWAY  BW. PARRY† 《Australian veterinary journal》1989,66(11):364-366

Blood samples from 13 cases of snakebite, 6 in dogs and 7 in cats, were tested for activated partial thromboplastin time (APTT), prothrombin time (PT) and fibrin/fibrinogen degradation products (FDP). Four cases were tested for fibrinogen concentration. Based on the results of a commercially available ELISA test, 9 cases were caused by tiger snakes (Notechis scutatus) and 1 case by a brown snake (Pseudonaja textilis). Three other cases had clinical signs and increased creatine phosphokinase values which suggested tiger snake envenomation. Although the period post-envenomation varied, results indicated a marked prolongation of the APTT and PT in 5 of 6 dogs. Three of these 5 dogs also had increased FDP values and 3 (of 3 examined) were hypofibrinogenaemic. Clinical manifestations of this coagulopathy were: haematoma formation after venepuncture (3 cases), gingival petechiae (1 case) and hyphaema (1 case). In contrast, there was minimal or no prolongation of the APTT and PT values, and no increase in FDP, in all 7 cats. Furthermore, no cat exhibited clinical signs of a coagulopathy.  相似文献