Nodular necrobiosis in a horse     

T. J. NICHOLLS  K. S. ARNOLD  K. G. JOHNSTON 《Australian veterinary journal》1983,60(5):148-148

  相似文献   

In vitro Developmental Competence of Adult Sheep Oocytes Treated with Roscovitine          下载免费PDF全文

LF Crocomo  F Ariu  L Bogliolo  D Bebbere  S Ledda  SD Bicudo 《Reproduction in domestic animals》2016,51(2):276-281

The efficiency of in vitro sheep embryo production is still low compared to that observed in vivo and in other species. In this context, meiotic inhibition strategies emerged as a promising alternative to improve this biotechnology. So, this study aimed to evaluate, for the first time, the effects of roscovitine on in vitro maturation of sheep oocytes and their subsequent embryo development. For this, cumulus–oocyte complexes (COCs) were cultured for 6 h in the presence (Rosco) or absence (Control) of 75 μm roscovitine and, subsequently, in vitro matured (IVM) for 18 h with gonadotropins. At 0 (Immature), 6 and 24 h of culture, the nuclear status of oocytes was evaluated by Hoechst staining. Embryo cleavage and blastocyst formation were recorded 30 h after in vitro fertilization and on day 7 of culture, respectively. Blastocyst quality was evaluated by differential staining. At 6 h, the GV rate in the Rosco treatment (93.8%) was similar to that observed in the Immature oocytes (94.9%) and significantly higher compared to Control (41.3%). After IVM for 18 h, a high and similar proportion of oocytes from Rosco (93.6%) and Control (88.4%) reached the MII stage. In both treatments, approximately 70% of oocytes cleaved and 50% of them developed up to blastocyst. The mean percentage of blastocyst cells, embryoblast, trophoblast and pyknosis did also not differ between Control and Rosco. In conclusion, roscovitine, at the studied experimental conditions, was efficient to reversibly inhibit the meiosis of adult sheep oocytes without detrimental effect on development and quality of the in vitro produced embryos.  相似文献   

Preliminary Trial: Motility Comparisons of a Unique Freezing Technology (UFT) to Liquid Nitrogen Mist Methodology for Cryopreservation of Porcine Spermatozoa     

HA Goolsby  JR Blanton  PZ Cotter  SD Prien 《Reproduction in domestic animals》2004,39(5):328-332

The motility outcomes of boar semen frozen with newly developed freezing techniques using a new unique freezing technology (UFT) compared with traditional liquid nitrogen methodology were investigated with the intent of improving current fertility outcomes using semen. The UFT is an electronically controlled cooling chamber that houses an organic fluid bath that can be maintained at temperatures below 0 degrees C without solidifying to freeze samples. Four ejaculates from four different boars were collected for this trial. Samples were handled consistently during the pre- and post-freeze processing. From each ejaculate, samples were separated into eight cryopreservation treatment groups, six UFT variations and two control liquid nitrogen groups, immediately before freezing, in replicates of two. After the initial cryopreservation was complete, all samples were stored in liquid nitrogen for at least 48 h. Post-thaw motilities and original motility return percentages were assessed on a random, individual-sample basis. After the initial evaluations, samples from two boars were recollected and frozen using the UFT for breeding purposes. Four sows were bred with the UFT frozen semen to confirm fertility capability. When assessing the individual UFT techniques, all of six UFT techniques had improved post-thaw motilities. However, treatments F (micro = 29%, return micro = 37%) and J (micro = 27%, return micro = 34%) showed the highest statistical improvement for post-thaw (p < 0.05) and original motility percent returns (p < 0.05) when compared with either the control cryo-tube (micro = 15%, return micro = 19%) or straw groups (micro = 12%, return micro = 16%). The UFT semen had a 50% conception rate, with an average of seven piglets from the sows that farrowed. Our preliminary data suggest a higher motility return with a slower pre-freeze phase below the freezing point before the acceleration to liquid nitrogen temperatures. The preliminary data suggest that the UFT could be utilized as a potential cryopreservation option for boar semen.  相似文献   

The Chinchilla Industry in Great Britain Today     

C. F. JOHNSTON 《The Journal of small animal practice》1963,4(3):189-195

Abstract— A review is presented of the development and growth of the chinchilla industry in Great Britain. The problems in the industry are examined with regard to costs of production, diseases, husbandry and improved stock and pelts.  相似文献   

Low-level counting methods for isotopic tracers     

JOHNSTON WH 《Science (New York, N.Y.)》1956,124(3226):801-807

  相似文献   

Molecular expression and identification of caprine estrogen receptor gene 1 for fertility status in bucks     

S Saraswat  SD Kharche  PK Rout  R Pawaiya  C Gangwar  DK Swain  R Kaushik 《Reproduction in domestic animals》2020,55(9):1080-1092

Estrogen and its receptors are essential for sexual development and reproduction. Oestrogen receptor alpha (ERα) is a nuclear receptor activated by the hormone oestrogen. In male, ERα is encoded by the gene ESR1 (oestrogen receptor1) responsible for better fertility. ESR1 is involved in the reabsorption of luminal fluid during the transit of spermatozoa from the testis to the head of the epididymis which is important for their survival and maturation during epididymal storage. The absence of ESR1 leads to reduced epididymal sperm content, reduced sperm motility and fertilizing ability. The present study was undertaken to investigate the expression and presence of ESR1 gene in fertile and low-fertile male goat breeds. We identified ESR1 gene through various molecular tools. Genotyping was carried out by high resonance melting analysis using Roche Light Cycler 480(LC-480) system and found three different genotypes. Genotypic frequency-AA (blue-0.67), BB(Red-0.2), AB(Green-0.08) with allele frequency A(0.71 and B (0.29). The predominance of this gene in head of epididymis in fertile bucks was confirmed by SDS-PAGE, Western blotting and immunohistochemistry. From the results, we corroborated that the present study provides a useful and effective way to predict male fertility in goat breeds, which in turn increases the percentage of fertility in flock leading to more number of offspring in a kidding season.  相似文献   

Transfer of Free Vascular Cutaneous Flaps by Microvascular Anastomosis Results in Six Dogs     

J. DAVID FOWLER DVM  MVSC    CRAIG W. MILLER DVM  MVSC  DiplomateACVs    VAUGHAN BOWEN MBChB  FRCS  GEOFFREY H. JOHNSTON MD  FRCS 《Veterinary surgery : VS》1987,16(6):446-450

Skin defects on the distal extremities of six dogs were reconstructed with free vascular cutaneous transfers by microvascular anastomosis. The donor flaps were based on the superficial cervical artery and vein. In five of the dogs, bone was exposed and skin was lost from half of the circumference of the limb. Two had infected fractures with sequestra and three had acute shearing injuries. The sixth dog had sensory denervation of the left antebrachium and a carpal acral lick granuloma. Before surgery, the patency of potential recipient vessels was confirmed with arteriography in five dogs and an ultrasonic doppler in one dog. Microvascular technique was used to reestablish circulation to the flaps after they were transferred to the recipient site. Total ischemic time of the flaps averaged 100 minutes. All flaps survived. Successful reconstruction of the cutaneous defects was achieved in these six cases.  相似文献   

Birth of a Banteng (Bos javanicus) calf at Western Plains Zoo after fixed time artificial insemination     

SD JOHNSTON  MR MCGOWAN  D. BLYDE 《Australian veterinary journal》2002,80(1):94-95

  相似文献   

Renal Allograft Survival in Outbred Mongrel Dogs Using Rabbit Anti-Dog Thymocyte Serum in Combination With Immunosuppressive Drug Therapy With or Without Donor Bone Marrow   总被引：1，自引：0，他引：1  

K.A. MATHEWS DVM  DVSc  Diplomate ACVECC    D.L. HOLMBERG DVM  MVSc  Diplomate ACVS    K. JOHNSTON DVM  MVSc    C.M. MILLER DVM  MVSc  Diplomate ACVS    A.G. BINNINGTON DVM  MSc  Diplomate ACVS    G. MAXIE DVM  PhD  Diplomate ACVP    M. ATILOLA dvm  PhD  G. SMITH MSc 《Veterinary surgery : VS》1994,23(5):347-357

Therapeutic renal transplantation in dogs is currently being investigated as a treatment for end-stage renal disease. This pilot study examines the effect of donor bone marrow (DBM) infusion and antithymocyte serum (ATS) in combination with immunosuppressive drug therapy in prolonging renal allograft survival in dogs. Seven normal outbred mongrel dogs received an unmatched renal allograft. All dogs received rabbit anti-dog thymocyte serum (RADTS), prednisone (Pr), cyclosporine-A (CsA) and azathioprine (Aza). In addition, three dogs (group 1 test) received DBM and four dogs (group 2 control) did not receive DBM. Serum CsA levels were measured throughout the study. Immunosuppressive therapy was gradually reduced with Pr, CsA, and Aza withdrawn at 200,450, and 680 days, respectively. Allograft rejection was treated with prednisolone sodium succinate. One dog in group 1 and one in group 2 died as a result of infectious canine rhinotracheitis and rejection early in the study. Renal allograft torsion occurred in one group 1 dog. The remaining four dogs survived the 2 years of the study. The dogs in group 2 (three dogs) all rejected the renal allograft after total drug withdrawal, the surviving dog in group 1 did not. This study demonstrates that RADTS, Pr, CsA, and Aza in combination can prolong renal allograft survival in mongrel dogs, whereas DBM may enhance the unresponsive state.  相似文献   

A small episode of single quarter conform infections in lactating dairy cows kept at pasture     

K. G. JOHNSTON  A. W. ENGLISH 《Australian veterinary journal》1984,61(11):364-366

For a period of 150 days, an Escherichia co/; infection in a single quarter of the udder of 13 cows occurred on average every 9 days, in a herd of 120 cows kept at pasture under temperate environmental conditions. Most cases were clinically mild, affecting cows in lactations 1 to 10 and at all stages of lactation; none were evident at calving. Samples of secretion from infected quarters sometimes had few demonstrable organisms. Eleven different serotypes in 8 (0) groups were recovered; one isolate was multiply resistant to antibiotics and one infection persisted for 120 days. A precise explanation for this episodic exacerbation of the new infection rate was not established, but probable contributing factors were recognised.  相似文献