Efficacy, duration, and onset of immunogenicity of a West Nile virus vaccine, live Flavivirus chimera, in horses with a clinical disease challenge model   总被引：1，自引：0，他引：1  

Long MT  Gibbs EP  Mellencamp MW  Bowen RA  Seino KK  Zhang S  Beachboard SE  Humphrey PP 《Equine veterinary journal》2007,39(6):491-497

REASON FOR PERFORMING STUDY: West Nile virus (WNF) is a Flavivirus responsible for a life-threatening neurological disease in man and horses. Development of improved vaccines against Flavivirus infections is therefore important. OBJECTIVES: To establish that a single immunogenicity dose of live Flavivirus chimera (WN-FV) vaccine protects horses from the disease and it induces a protective immune response, and to determine the duration of the protective immunity. METHODS: Clinical signs were compared between vaccinated (VACC) and control (CTRL) horses after an intrathecal WNV challenge given at 10 or 28 days, or 12 months post vaccination. RESULTS: Challenge of horses in the immunogenicity study at Day 28 post vaccination resulted in severe clinical signs of WNV infection in 10/10 control (CTRL) compared to 1/20 vaccinated (VACC) horses (P<0.01). None of the VACC horses developed viraemia and minimal histopathology was noted. Duration of immunity (DPI) was established at 12 months post vaccination. Eight of 10 CTRL exhibited severe clinical signs of infection compared to 1 of 9 VACC horses (P<0.05). There was a significant reduction in the occurrence of viraemia and histopathology lesion in VACC horses relative to CTRL horses. Horses challenged at Day 10 post vaccination experienced moderate or severe clinical signs of WNV infection in 3/3 CTRL compared to 5/6 VACC horses (P<0.05). CONCLUSIONS: This novel WN-FV chimera vaccine generates a protective immune response to WNV infection in horses that is demonstrated 10 days after a single vaccination and lasts for up to one year. POTENTIAL RELEVANCE: This is the first USDA licensed equine WNV vaccine to utilise a severe challenge model that produces the same WNV disease observed under field conditions to obtain a label claim for prevention of viraemia and aid in the prevention of WNV disease and encephalitis with a duration of immunity of 12 months.  相似文献   

Characterization,phylogenetic analyses and pathogenicity of Bipolaris setariae associated with brown stripe disease on sugarcane in Yunnan,China     

Jie Li  Rongyue Zhang  Yinhu Li  Chaohua Long  Wengfeng Li  Hongli Shan  Wenjie Lu  Yingkun Huang 《Plant pathology》2023,72(1):89-99

Brown stripe disease is a severe foliar fungal disease of sugarcane worldwide and is widespread in all sugarcane planting areas in China. Brown stripe is a major disease that seriously affects the output and quality of the sugarcane industry in Yunnan Province, China's second-largest sugar base, while the pathogen of this disease remains not yet fully understood. To address this, we isolated and identified the fungi associated with 68 leaf samples showing typical symptoms of brown stripe from 22 sugarcane varieties in different areas of Yunnan Province. A total of 113 isolates were obtained, which were morphologically similar. Of these, 64 representative isolates were sequenced for the internal transcribed spacer region (ITS), GAPDH and EF-1α loci. All representative isolates grouped with the type strain of Bipolaris setariae in the phylogenetic trees inferred with individual and concatenated sequences of ITS, GAPDH and EF1-α. Pathogenicity test results showed that B. setariae strains were able to induce typical symptoms of brown stripe. The results obtained in this study clarify that only B. setariae is associated with sugarcane brown stripe in Yunnan, China. It is recorded here for the first time as a pathogen causing sugarcane brown stripe in Yunnan, and it is able to infect many major cultivars and new varieties, posing a new threat to the sugar industry in Yunnan Province. In addition, these results provide the scientific basis for the future breeding of disease-resistant varieties and effective prevention and control of sugarcane brown stripe disease.  相似文献   

湿地松优良半同胞家系蛋白质及糖类对水分胁迫的生理响应   总被引：3，自引：0，他引：3  

童方平  方伟  马履一  宋庆安  龙应忠  吴际友  易霭琴  程勇 《中国农学通报》2006,22(12):459-459

为研究湿地松优良半同胞家系蛋白质及糖类对水分逆境的生理响应，采用盆栽试验和吸光度测定结合的方法，以湿地松普通种子为对照（CK），测定了七个湿地松半同胞家系在正常水分状态（土壤相对含水量为70%）、弱度水分胁迫（土壤相对含水量为55%~60%）、中度水分胁迫（土壤相对含水量为35%~40%）和强度水分胁迫（土壤相对含水量为20%~25%）条件下的可溶性蛋白质、还原性糖、可溶性糖的含量，研究结果表明：无论是不同水分梯度还是不同家系水平，蛋白质、还原性糖、可溶性糖含量的差异均达极显著性水平。随着水分胁迫程度的加剧，各家系蛋白质含量呈先升后降的趋势，在水分胁迫初期，各家系蛋白质含量增加，水分胁迫中、后期各家系蛋白含量呈直线下降；随着水分胁迫的加剧，各家系还原性糖、可溶性糖含量呈直线上升。家系1027、101、464在水分胁迫条件下具有较高的可溶性糖含量，其抗旱性较其它家系要强，是这三个家系速生、高产的生理原因之一。  相似文献   

Effects of Low- Intensity Ultrasound Treatment on Excess Sludge     

DING Wen - Chuan  LONG Teng - Rui  XU Long  LI Li  LIAN He - chuan 《保鲜与加工》2006,(3):74-77

In this study the influence of the low-intensity ultrasound treatment on excess sludge has been observed.The oxygen uptake rate(OUR),soluble chemical oxygen demand(SCOD),soluble total nitrogen(TN) and soluble total phosphorus(TP) were examined for three power levels of 0.03 W/mL, 0.05 W/mL and 0.1 W/mL as well as two time series of 0~5 min and 0~30 min.It showed that there were two stages of ultrasonic treatment: the first stage took place within the flocs in four minutes,in which SCOD,TN,TP were dissolved from flocs.In the second stage,the microbial activities have been strengthened,as well as OUR,TN,TP have increased greatly.  相似文献   

Reuolution and Research of Green Automobile     

RAN Zheng -y  TIAN Long  NI Ling  YANG Chao  CAO Wen -ming 《保鲜与加工》2002,(7):15-19

The green is as a symbol of no pollution,no public harm and environmental protection. The green automobile is the inevitable results that the automobile technique continuously developes. A new style vehicle--the green automobile is the urgent solicit of environmental protection is developed. The automobile industry can develop continuously. This article mainly discusses the importance of the green auto_mobile's research and development,the development status in quo and prospect of the green auto_mobile technique in the world,and states the relationship between the green automobile technique and environmental protection from green technical system theoretically clarify the study of green automobile,including its design and the system,the manufacture and its technology,the energy,the rebuild engineering and a series of subjects. How to evaluate the cycle and the evaluable mark of the green automobile,the prospect of green automobile are pointed out.  相似文献   

CALCULATION OF TNE END-EFFECT FACTOR OF SYNCHRONOUS MOTOR WITH CYLINDRICAL SOLID ROTOR     

Xiao Tiyan  Long Lili 《保鲜与加工》1994,(4):83-88

This paper presents the end-effect factor of Synchronous motor with cylindricalsolid rotor offered by analysing the 3-D electromagnetic field and using the Poynting vector.To ap-ply it in an actual calculation,the end-effect factor is proved correct and applicable.  相似文献   

Study on Application Model of Knowledge Expression     

ZHU Xiao-hong  HU Yong  ZENG Long  XU Zong-jun 《保鲜与加工》2005,(2):121-123

As a key resource, the knowledge management is becoming a research field interested by many researchers. In order to study the mathematics theory base of knowledge expression, in this paper, a knowledge expression model is given. In this model the knowledge system is expressed by multi-tuple, the single knowledge object is expressed by the tuple, a structural expression of knowledge is given using token as example and the application of the model in city water supply pipe network is presented in this paper.  相似文献   

Real-time Performance of Linux System     

JIANG Yi  LI Lin-hao  CHEN Long  XIONG An-ping 《保鲜与加工》2005,(3):61-64

The standard Linux is a typical time-sharing system, it has a poor real-time performance. But according to the applications of the Linux OS are expanding on real-time domain, it becomes necessary to enhance the performance of the Linux system. This paper analyses the schedule policies and schedule algorithms of the Linux kernel, and discusses several different kinds of methods about enhancing real-time performance of the Linux system from the total Linux system. They authors summarizes the current research's developments of the Linux system.  相似文献   

小黄鱼热泵干燥工艺及设备选型分析     

吴耀森  刘军  李浩权  龙成树  龚丽 《现代农业装备》2015,(2):37-42

研究了不同热泵干燥温度下小黄鱼的干燥特性,并对其干燥品质进行分析。结果表明在干燥温度55℃、铺放密度为6.48 kg/m2的条件下干燥时间为13.5 h,干燥品质最优。在综合考虑能耗、企业加工条件和产品品质的前提下,将生产工艺参数确定为:干燥温度60℃、铺放密度为6~7 kg/m2,干燥时间为10~12 h。根据企业日处理量5.5~6.0 t的加工要求,通过对产能核算及加工成本计算,确定设备选型方案为:1台GHRH-510型热泵干燥机与2台GHRH-340型热泵干燥机,该方案投资回报期短,社会效益明显。  相似文献   

水稻田间育秧精密播种机设计与试验     

马旭  邝健霞  齐龙  梁仲维  谭永炘  江立凯 《农业机械学报》2015,46(7):31-37

为提高田间育秧播种机的播种合格率和播种稳定均匀性,设计了一种适用于南方水稻田间育秧的精密播种机。在深入分析螺旋勺轮式播种器的播种原理,以及播种器行走控制系统的基础上,采用正交试验方法,研究了螺旋勺轮的凹槽深度、螺旋升角、播种器行走速度对播种合格率和空穴率的影响规律,得出杂交稻播种合格率和空穴率影响因素的最佳参数组合:凹槽深度h为3 mm、螺旋升角α为81.73°、播种器行走速度v为0.35 m/s。试验表明,采用闭环控制系统后的播种机播种杂交稻2~6粒/格合格率由87.14%提升到93.21%,常规稻3~8粒/格的播种合格率为92.14%,播种性能满足常规稻和杂交稻精密播种育秧的技术要求。  相似文献