Efficiency assessment of the antifungal metabolites from Sclerotium cepivorum against onion white rot disease     

Elsherbiny A. Elsherbiny  Amany S. Saad  Mona G. Zaghloul  Mohamed A. El-Sheshtawi 《European journal of plant pathology / European Foundation for Plant Pathology》2015,142(4):843-854

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Effects of partial and complete replacement of soybean meal with cottonseed meal on growth,feed utilization and haematological indexes for mono‐sex male Nile tilapia,Oreochromis niloticus (L.) fingerlings     

Deyab M S D El‐Saidy  Amal S Saad 《Aquaculture Research》2011,42(3):351-359

The main objective of this study was to evaluate the effects of replacing soybean meal (SBM) with cottonseed meal (CSM) on the growth performance, feed utilization and haematological parameters of mono‐sex male Nile tilapia fingerlings. Five isonitrogenous diets (containing 31.82% crude protein) containing graded levels of CSM to replace SBM protein were fed to triplicate groups of fish. The diets were supplemented with lysine so that they were similar to the control diet. After a 14‐week feeding experiment, the results revealed that up to 75% of SBM could be replaced by CSM without causing a significant reduction in growth. Fish fed the diet highest in CSM had a significantly lower protein efficiency ratio and a significantly higher feed conversion ratio than fish fed the other diets. High survival was observed in all the dietary treatments, and no significant difference was observed among treatments. The apparent digestibility coefficients (ADC) of dry matter and phosphorus decreased significantly with an increase in the dietary CSM level, whereas the ADC of lipid was not affected by the dietary treatment. The hepatosomatic index and the condition factor were significantly affected by the replacement of SBM by CSM. No significant differences were detected in the moisture, lipid and ash content in whole body and muscle samples, but protein in whole‐body samples was significantly affected by the CSM levels. Significant differences were found in the haemoglobin, haematocrit, red blood cell and white blood cell contents in fish fed diets with different CSM levels. Therefore, these findings suggest that up to 41.25% CSM can be used to replace 75% of SBM protein in diets for mono‐sex male Nile tilapia fingerlings without any adverse effects on the growth performance, feed utilization, body composition and haematological indexes.  相似文献   

Schistosoma bovis whole egg antigen did not protect Zebu calves against experimental schistosomosis     

Imadeldin E. Aradaib  Osama H. Omer  Babiker B. Abbas  Hamid O. Bushara  Khitma H. Elmalik  Amir M. Saad  Bennie I. Osburn  Martin G. Taylor 《Preventive veterinary medicine》1995,21(4):339-345

Six calves were immunized with whole egg antigen of Schistosoma bovis emulsified in Freund's adjuvant. The immune response was monitored by agar-gel immunodiffusion (AGID) and enzyme-linked immunosorbent assay (ELISA). Using AGID and ELISA, antibodies to whole egg antigen were detected in sera from all immunized calves, but not in sera from control calves. The immunized calves and six control calves were challenged 6 months after the beginning of the immunization with 20 000 cercariae of Schistosoma bovis administered percutaneously to the shaved tail. There was no significant difference between the immunized and the control calves as judged by fecal and tissue egg counts, worm recovery and hematological parameters. There was a lack of association between antibody production and protection.  相似文献   

Control of ovine brucellosis in najdi sheep in saudi Arabia     

Ahmed I. Radwan  Saad I. Bekairi  Abdullah A. Al-Mukayel  Osama S. Abu Agla 《Tropical animal health and production》1984,16(4):213-218

Summary A practical programme for control of ovine brucellosis in a Najdi sheep flock at Haradh and protection of sheep handlers from infection was implemented. Haradh sheep were serologically tested three times at six-month intervals. Of 2,721 sheep subjected to the first test, 387 (14.2%) were positive reactors. At the second test 2,072 sheep were tested and 31 (1.5%) were positive. The third test was performed on 2,963 sheep and only nine (0.3%) positive reactors were found. Of eight sheep handlers six had Malta fever. Positive reactor sheep were slaughtered and buried after each test. Following the third test all negative sheep were vaccinated with inactivatedBrucella melitensis vaccine. Infected sheep handlers were treated at the Haradh clinic. One year after completion of the programme abortions and infertility problems had disappeared and the lambing percentage had increased from 0.8 to 1.3 lambs per ewe. In addition all young lambs born to vaccinated ewes were found to be serologically negative. We suggest expansion of the present programme into a national brucellosis control campaign in the kingdom.

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Control De Brucelosis Ovina En Ovejas Najdi En Arabia Saudita

Resumen Se implementó un programa práctico, para el control de brucelosis ovina en ovejas Najdi en Haradh, y para proteger a los pastores. Las ovejas se analizaron serológicamente tres veces con intervalos de seis meses. De 2.721 ovejas analizadas la primera vez, 387 (14.2%) dieron resultados positivos. La segunda vez, se analizaron los sueros de 2.072 ovejas, de las cuales 31 (1.5%) fueron positivas. El tercer analisis se realizo en 2.963 ovejas de la cuales solo nueve (0.3%) se detectaron como reactores positivos. De ocho pastores, seis tenían fiebre de Malta. Las ovejas positivas fueron eliminadas después de cada prueba serológica. Seguidamente, después de la tercera prueba, todas las ovejas negativas se vacunaron con la vacuna inactivada deBrucella melitensis. Los pastores enfermos, fueron hospitalizados. Un año después de la campaña, los problemas de aborto e infertilidad habían desaparecido, y el porcentaje de nacimientos se incrementó de 0.8 a 1.3 corderos por oveja. Más aún, todos los corderos nacidos de madres vacunadas fueron negativos serológicamente. Nosotros sugerimos la expansión del presente programa de control de brucelosis, a todo el Reino.

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Lutte Conter La Brucellose Ovine Chez Des Moutons Najdi En Arabie Saoudite

Résumé Un programme pratique de lutte contre la brucellose ovine dans un troupeau de moutons Najdi à Haradh et de protection des éleveurs contre l'infection a été mis en oeuvre. Les moutons de Haradh ont été testés sérologiquement trois fois à six mois d'intervalle. Sur 2 721 moutons soumis au premier test, 387 (14,2 p. 100) ont réagi positivement; sur 2 072 moutons, au second test, 31 (1,5 p. 100); et sur 2 963 moutons, au troisième test, seulement 0,3 p. 100. Sur huit éleveurs de moutons, six étaient atteints de fièvre de Malte. Les moutons ayant réagi positivement ont été abattus et enterrés après chaque test. Après le troisième test, tous les animaux négatifs ont été vaccinés avec un vaccin àBrucella melitensis inactivé. Les éleveurs ont été soignés la clinique de Haradh. Un an après la fin du programme, les avortements et les problèmes d'infertilité avaient disparu et le pourcentage d'agnelages avait augmenté de 0,8 à 1,3 agneau par brebis. De plus, tous les jeunes agneaux nés de mères vaccinées étaient sérologiquement négatifs. L'extension de ce programme en une campagne de lutte nationale est préconisée.

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Mycoplasmas isolated from goats and sheep with pleuropneumonia in Saudi Arabia     

Ahmed I. Radwan  Nabil M. Al-Zeftawi  Mohamed A. Al-Issa  Saad I. Bekairi  Abdullah A. Al-Mukayel 《Tropical animal health and production》1985,17(4):233-238

Summary Pleuropneumonia of goats and sheep has been known to cause serious economic losses in many countries including Saudi Arabia. A preliminary investigation was carried out on 163 goats and 130 sheep which showed clinical symptoms or post-mortem lesions of pleuropneumonia. Pathological samples from the respiratory tract of live or slaughtered goats and sheep (183 and 160 samples respectively) were cultured for mycoplasmas. Of 343 mycoplasma cultures examined 211 (61·5%) isolates were characterised biochemically. A total of 116 isolates were serologically identified asM. mycoides subsp.mycoides, M. ovipneumonia, M. conjunctivae, M. agalactiae subsp.agalactiae, M. putrifaciens andM. arginini. The remaining mycoplasma isolates were not identified. The significance of the identified isolates and their probable pathogenic effect on the respiratory tract of goats and sheep are discussed. Their pathogenicity needs to be established experimentally before initiating a control programme in the Kingdom.

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Resumen Se llevó a cabo una investigación preliminar en 163 cabras y 130 ovejas las cuales mostraron síntomas clínicos o lesiones postmortem de pleuroneumonía contagiosa. Se cultivaron en medios especiales muestras patológicas del tracto respiratorio de cabras y ovejas vivas (183 y 160 muestras respectivamente. De 343 cultivos examinados, 211 (61·5%) aislamientos se caracterizaron bioquímicamente. Un total de 116 aislamientos se caracterizaron serológicamente comoM. mycoides, subsp.mycoides, M. ovipneumonia, M. conjuntivae, M. agalactiae subsp.agalactiae, M. putrifaciens yM. arginini. Los restantes micoplasmas aislados no fueron identificados. Se discute el significado de los aislamientos identificados y su posible efecto patógeno en el tracto respiratorio de cabras y ovejas. Es necesario establecer la patogenicidad experimentalmente antes de iniciar un programa de control en el Reino.

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Résumé On sait que la pleuropneumonie du mouton et de la chèvre cause de sérieuses pertes économiques dans beaucoup de pays, dont l'Arabie Saoudite. Une recherche préliminaire a été entreprise sur 163 chèvres et 130 moutons montrant des sympt?mes cliniques ou des lésions à l'autopsie. Des prélèvements du tractus respiratoire de chèvres et de moutons vivants ou abattus (respectivement 183 et 160 échantillons) ont été mis en culture pour la recherche de mycoplasmes. Sur les 343 cultures de mycoplasmes examinées, 211 souches (soit 61,5%) ont été caractérisées au point de vue biochimique. 116 souches ont pu être identifiées sérologiquement comme étantM. mycoides sub.mycoides, M. ovipneumoniae, M. conjunctivae, M. agalactiae sub.agalactiae, M. putrefaciens etM. arginini. Les autres souches de mycoplasmes n'ont pas été identifiées. La signification des souches identifiées et leur effet pathogène probable sur le tractus respiratoire des chèvres et des moutons sont discutés. Leur pouvoir pathogène demande à être expérimentalement établi avant de mettre en place un programme de contr?le de la maladie dans le Royaume.

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Immunological changes during pregnancy in the viviparous lizard, Chalcides ocellatus     

A H Saad  S el Deeb 《Veterinary immunology and immunopathology》1990,25(3):279-286

Splenic cells from pregnant and non-pregnant viviparous lizards (Chalcides ocellatus) were stimulated in vitro with the mitogens, concanavalin A (Con A), phytohemagglutinin (PHA) and Escherichia coli lipopolysaccharide (LPS). Cell cultures from pregnant animals were significantly less responsive to Con A and PHA than comparable cultures from non-pregnant animals. The response was depressed during the first period of pregnancy and remained low in magnitude until parturition. By contrast, the response of maternal splenic cells to LPS was reduced in pregnant lizards only during advanced pregnancy. The drastic decrease in mitogenic responsiveness was associated with marked involution of the maternal spleen. These findings strongly suggest that pregnancy impairs the immunoreactivity of viviparous lizards. Possible mechanisms for this impairment and the relationship to circulating levels of sex hormones are discussed.  相似文献   

Prevalence and distribution pattern of intestinal helminths in chicken and pigeons in Aswan,Upper Egypt     

El-Dakhly  Khaled Mohamed  El-Seify  Mahmoud A.  Mohammed  Eman Sayed  Elshahawy  Ismail Saad  Fawy  Samia Abdel-Motaleb  Omar  Mossab A. 《Tropical animal health and production》2019,51(3):713-718

Tropical Animal Health and Production - There is scarce data available on helminth infection of poultry in the region of Aswan province, Upper Egypt. A total of 276 poultry (156 baladi chickens and...  相似文献   

The effects of dietary tomato powder (Solanum lycopersicum L.) supplementation on the haematological,immunological, serum biochemical and antioxidant parameters of growing rabbits     

Hamada A. M. Elwan  Shaaban Saad Elnesr  Mohamed Mohany  Salim S. Al-Rejaie 《Journal of animal physiology and animal nutrition》2019,103(2):534-546

Using nutritional antioxidants in livestock systems is considered the key in improving animal production. The current study assumes that dietary tomato powder (TP) supplementation positively affects haemato‐immunological, biochemical, and antioxidant parameters for New Zealand rabbits. A total of 30 rabbits (45 days old) were assigned to three groups, including a diet with no additives (control), and two dietary treatments with the providing of 1% or 2% TP. Mass spectrometric study for TP methanolic extract showed some phenolic compounds. Consumption of TP supplemented diets significantly (p < 0.001) affected body weight gain and feed efficiency. Red blood cells and white blood cells count exhibited a significant increase (p < 0.001) in both TP groups compared with the control. In addition to, feeding rabbits on TP enhanced cell‐mediated and humoral immune responses through a significant increase in phagocytosis, chemotaxis, and levels of immunoglobulins (TIg, IgG, IgM and IgA). Supplementation of TP significantly (p < 0.01) reduced lipid profile induces except high‐density lipoprotein cholesterol values. A remarkable significant (p < 0.001) effect on serum and hepatic oxidative stress responses were observed with TP addition. Ultimately, TP supplementation could play a potential role as a growth and health enhancer for fattening rabbits.  相似文献   

A report of mycobacteriosis caused by Mycobacterium marinum in bullfrogs (Rana catesbeiana)     

Ferreira R  Fonseca Lde S  Afonso AM  da Silva MG  Saad MH  Lilenbaum W 《Veterinary journal (London, England : 1997)》2006,171(1):177-180

The occurrence of mycobacteriosis caused by Mycobacterium marinum in a commercial breeding farm of bullfrogs (Rana catesbeiana) in Rio de Janeiro, Brazil is described. Ten animals presented skin lesions on the head and extremities. These and 38 other asymptomatic adult animals from various tanks were killed and at necropsy disseminated granulomatous lesions were observed in the 10 clinically affected animals and in 16 (42.1%) of the asymptomatic frogs. Acid-fast bacilli were observed in all smears of the 10 symptomatic frogs and in all but one from the 16 asymptomatic animals with visceral lesions. Ten samples from the 25 positive animals were randomly selected for culture which yielded four isolates of fast-growing (<7 days) mycobacteria. Those purified isolates were characterised by biochemical traditional means as M. marinum. Identification of the strains was confirmed using reverse-phase high-performance liquid chromatography and a polymerase chain reaction (PCR) restriction enzyme analysis assay. It is suggested that M. marinum is an important agent of granulomatous disease in bullfrogs and that infected animals, even when asymptomatic, could act as reservoirs spreading the disease and contaminating other frogs in the farm.  相似文献   

Effect of pollution on the blood characteristics ofTilapia zillii G.     

Massodd A. H. Saad  A. Ezzat  M. B. Shabana 《Water, air, and soil pollution》1973,2(2):171-179

Lake Mariut, a shallow brackish water basin near Alexandria, was a highly productive lake; however, it now receives large quantities of pollutants. The amount of DO has been greatly reduced.Tilapia zillii G. have been studied to determine the modification of blood characteristics. In O₂ deficient areas of Lake Mariut the fish undergo asphyxiation.  相似文献