Antiproliferative activity of violaxanthin isolated from bioguided fractionation of Dunaliella tertiolecta extracts     

Pasquet V  Morisset P  Ihammouine S  Chepied A  Aumailley L  Berard JB  Serive B  Kaas R  Lanneluc I  Thiery V  Lafferriere M  Piot JM  Patrice T  Cadoret JP  Picot L 《Marine drugs》2011,9(5):819-831

Dunaliella tertiolecta (DT) was chemically investigated to isolate molecules inhibiting cancer cell proliferation and inducing apoptosis in vitro. The potency to inhibit cell growth was used for the bio-guided fractionation and isolation of active compounds using chromatographic techniques. The DT dichloromethane extract exhibited a strong anti-proliferative activity on MCF-7 and LNCaP cells, and was further fractionated and sub-fractionated by RP-HPLC. High resolution mass spectrometry and spectrophotometric analysis unequivocally identified violaxanthin as the most antiproliferative molecule present in DT DCM extract. Violaxanthin purified from DT induced MCF-7 dose-dependent growth inhibition in continuous and discontinuous treatments, at concentrations as low as 0.1 μg·mL⁻¹ (0.17 μM). Phosphatidylserine exposure, typical of early apoptosis, was observed after 48 h treatment at 8 μg·mL⁻¹ (13.3 μM) but no DNA fragmentation, characteristic of late apoptosis steps, could be detected even after 72 h treatment at 40 μg·mL⁻¹ (66.7 μM). Taken together, our results demonstrate the strong antiproliferative activity of violaxanthin on one human mammary cancer cell line, and suggest that studying the pharmacology of violaxanthin and pharmacomodulated derivatives on cancer cells may allow potent antiproliferative drugs to be obtained.  相似文献   

Genetic variation and population structure of the caramote prawn Penaeus kerathurus (Forskäl) from the eastern and western Mediterranean coasts in Tunisia     

Rym Zitari-Chatti  Noureddine Chatti  Ali Elouaer  & Khaled Said 《Aquaculture Research》2008,39(1):70-76

Ten populations of Penaeus kerathurus (Forskäl), a prawn of high commercial value, were sampled from the eastern and western Mediterranean coastal waters of Tunisia and screened electrophoretically for genetic variation at 13 allozyme loci. Four among the six polymorphic loci were out of Hardy–Weinberg equilibrium (H–WE) in at least one population. In the same way, the multilocus test showed deviation from H–WE in six populations. These populations showed heterozygote deficiency whereas the average heterozygosity for the four remaining ones is quite similar to the expected levels. Genetic variability was low. The number of alleles per locus ranged from 1.2 to 1.5 (average=1.3), and the observed heterozygosity varied between 0.010 and 0.048 (average=0.021). Significant population differentiation (F_ST=0.076, P<0.05) in the total data set reflected the differentiation of the two populations, which were at the margins of the range sampled, from all the others (Pairwise F_ST values ranged from 0.035 to 0.208). Although there was no significant differentiation among the other populations (pairwise F_ST values ranged from ?0.006 to 0.201, P>0.05). Our data suggest a population structure consistent with separation by Mediterranean Sea basins that might reflect different local biogeographical zones.  相似文献   

Diallel analysis for morphoagronomic descriptors in Physalis angulata L. hybrids     

de Souza Farias  Jos&#; Williano  Orella  Jonathan Said Tejeda  dos Santos Batista  Edgar  Cordeiro  Rafael Cruz  Passos  Adriana Rodrigues  Silva  Luiz Cl&#;udio Costa 《Genetic Resources and Crop Evolution》2022,69(3):1365-1374

Genetic Resources and Crop Evolution - Physalis angulata L. is an American species with edible fruits that stands out for having high nutritional and pharmaceutical value. The species is found in...  相似文献