Molecular detection of human periodontal pathogens in oral swab specimens from dogs in Japan     

Kato Y  Shirai M  Murakami M  Mizusawa T  Hagimoto A  Wada K  Nomura R  Nakano K  Ooshima T  Asai F 《Journal of veterinary dentistry》2011,28(2):84-89

Periodontal diseases are known to be major diseases in humans, and are also common in dogs. The purpose of the present study was to analyze the distribution of periodontitis-related bacterial species using oral swab specimens collected from 26 pet dogs. The distribution of an animal gingival organism Porphyromonas gulae, in addition to 10 human periodontitis-related bacterial species, including Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, Capnocytophaga ochracea, Capnocytophaga sputigena, Prevotella intermedia, Prevotella nigrescens, Aggregatibacter actinomycetemcomitans, Campylobacter rectus, and Eikenella corrodens, were evaluated by polymerase chain reaction with species-specific sets of primers. Porphyromonas gulae, Tannerella forsythia and Campylobacter rectus were detected in almost all dogs analyzed, all of which should be regarded as common members of oral flora in dogs. Then, isolation and identification of the Porphyromonas species in swab specimens were performed. There were 35 strains isolated from 22 dogs, and broad-range polymerase chain reaction and sequencing methods revealed that approximately 70% of them were Porphyromonas gulae. In contrast, the frequency of Porphyromonas gingivalis was extremely low. These findings indicate the presence of specific periodontitis-related pathogens in pet dogs, especially Porphyromonas gulae.  相似文献   

Developmental delay in islet-1-positive motor neurons in chick spina bifida     

Wang M  Mominoki K  Kinutani M  Wang Z  Kobayashi N  Shimokawa T  Nabeka H  Fujiwara T  Matsuda S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(4):447-452

Spina bifida aperta (SBA) is a congenital malformation of the spinal cord with complications such as spinal ataxia and bowel and bladder dysfunction. We have developed a chick model with surgery-induced SBA that shows spinal ataxia after hatching. In the present study, motor neurons in the early stages in chicks with and without SBA were observed by immunohistochemical staining with a monoclonal antibody against Islet-1, a motor neuron marker. Delay in migration and maturation of motor neurons was observed in SBA. Although the final numbers of Islet-1-positive neurons in these two groups were not different, a defect in the production and elimination of excess motor neurons in the early developmental stages in the SBA group may be involved in the pathological mechanism of the motor complications of this disease.  相似文献   

Acute myelomonocytic leukemia (AML-M4) in a dog with the extradural lesion     

Hayashi A  Tanaka H  Kitamura M  Nishida H  Shimoda T  Ohashi F  Nakayama M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(3):419-422

A two-year-old dog having presented with neurological signs showed marked leukocytosis and appearance of blast cells in the peripheral blood. Hematological and bone marrow examination showed an increase in blasts having both myeloid and monocytic cells characteristics. The dog was diagnosed with acute myelomonocytic leukemia (AML-M4) on the basis of bone marrow findings. Although the dog was treated with a multi-combination chemotherapy, the neurological abnormalities progressed and the dog was euthanized. Myelographic examination and necropsy revealed the extradural lesion formed by AML-M4 around the cervical spinal cord and this lesion was considered as a cause of the neurological signs.  相似文献   

Comparisons of Internal Behavior after Exposure to Flavobacterium psychrophilum between Two Ayu (Plecoglossus altivelis altivelis) Strains Showing Different Cumulative Mortality to Bacterial Cold Water Disease     

Tetsushi KAGEYAMA  Tomonori KUWADA  Kenichi OHARA  Aya NOUNO  Tetsuya UMINO  Shuichi FURUSAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(12):1571-1575

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PREOPERATIVE RADIOTHERAPY FOR VACCINE ASSOCIATED SARCOMA IN 92 CATS     

Tetsuya  Kobayashi  DVM  Marlene L.  Hauck  DVM  PhD  Richard  Dodge  MS  Rodney L.  Page  DVM  MS  G. Sylvester  Price  DVM  PhD  Laurel E.  Williams  DVM  Elizabeth M.  Hardie  DVM  PhD  Kyle G.  Mathews  DVM  MS  Donald E.  Thrall  DVM  PhD 《Veterinary radiology & ultrasound》2002,43(5):473-479

Medical records for 92 cats with a vaccine associated sarcoma receiving preoperative irradiation, with or without chemotherapy, between December 1985 and September 1998 were reviewed. The purposes were to quantify response to treatment and to attempt identification of factors associated with favorable response. Variables evaluated for a relationship to outcome included signalment, tumor location, presence of gross vs. microscopic tumor, radiation field size, irradiation technique, type of surgical procedure, completeness of excision, and chemotherapy (none, carboplatin alone, and others). Time to first event was calculated for the first day of treatment until local tumor recurrence or metastasis, or the date of euthanasia or death. Median time to first event for all 92 cats was 584 days. Only completeness of surgical excision was related to the time to first event. Median time to first event in cats having complete surgical excision was 986 days compared to 292 days for cats with incomplete excision (P = 0.004). Cats requiring bone removal to effect tumor removal had earlier failure than cats having other types of surgery. There was not a significant relationship between administration of chemotherapy or chemotherapy type and time to first event although outcome in cats receiving carboplatin was better than all other treatment groups. Carboplatin addition to preoperative irradiation appears worthy of further study. Preoperative irradiation is an effective treatment for cats with vaccine associated sarcoma, especially if complete excision can be accomplished following irradiation.  相似文献   

Vibrational properties of wood plastic plywood     

Yingcheng Hu  Tetsuya Nakao  Takahisa Nakai  Jiyou Gu  Fenghu Wang 《Journal of Wood Science》2005,51(3):315

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Deformity of agglutinated pelvic fin membrane in hatchery-reared black rockfish Sebastes inermis and its application for stock separation study     

Tomoya  MURAKAMI  Satoshi  AIDA  Kouji  YOSHIOKA  Tetsuya  UMINO  Heisuke  NAKAGAWA 《Fisheries Science》2004,70(5):839-844

ABSTRACT:   This study is the first to report on the high occurrence of agglutinated pelvic fin membrane deformities in hatchery-reared black rockfish Sebastes inermis . For 5 years, this symptom was marked in hatchery-reared fish, with 58.7% of fish deformed on average (varying between 46.7 and 72.0%). The deformity was a peculiarity in hatchery-reared fish, but is negated in wild fish, and was not related to whether the fish broodstock originated from the wild or from a hatchery. Mark–release experiments showed that deformed fish were almost the same as normal hatchery-reared fish in growth and survival rates, and, theoretically, the recapture numbers of hatchery-stocked fish, estimated by deformity, almost coincided with actual recapture numbers, confirmed by otolith tagging. The results of the present study indicate that deformity in hatchery-reared black rockfish is useful as a stock separation tool.  相似文献   

Assessment of meibomian gland morphology by noncontact infrared meibography in Shih Tzu dogs with or without keratoconjunctivitis sicca     

Yasunari Kitamura  Seiya Maehara  Tetsuya Nakade  Yukihiro Miwa  Reiko Arita  Hiroko Iwashita  Akihiko Saito 《Veterinary ophthalmology》2019,22(6):744-750

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Patterns of species diversity in rural herbaceous communities under different management regimes, Chiba, central Japan     

Tetsuya Kitazawa  Masahiko Ohsawa 《Biological conservation》2002,104(2):239-249

The patterns of plant species diversity in herbaceous vegetation subjected to various human activities were studied in most of the landscape elements in a rural area of Chiba, central Japan. Twenty-eight transects were sampled in four types of human management-regime (cultivation, trampling, mowing, and abandonment) and were grouped into seven vegetation types using TWINSPAN and DCA analyses. The DCA axis 1 arranged all the transects into a successional order along which biomass and the degree of succession increased. Accumulated number of species increased in a stepwise pattern along the DCA axis 1, in which the dominant plant life-forms were replaced from annuals, to perennials and perennials/tree-saplings depending on different management regimes. The unique species which were confined to a certain management regime, were identified in each site. It is suggested that the coexistence of various successional communities under different human management regimes enhance regional species diversity through maintaining these unique species. Among four types of management regime, mowing sites (especially traditional verge meadows) had most abundant unique, rare species specially adapted to regular cutting. It is suggested that maintaining such traditional mown sites is important to conserve the unique biodiversity of the studied area.  相似文献   

Antibacterial activity of coriander volatile compounds against Salmonella choleraesuis     

Kubo I  Fujita K  Kubo A  Nihei K  Ogura T 《Journal of agricultural and food chemistry》2004,52(11):3329-3332

Aliphatic (2E)-alkenals and alkanals characterized from the fresh leaves of the coriander Coriandrum sativum L. (Umbelliferae) were found to possess bactericidal activity against Salmonella choleraesuis ssp. choleraesuis ATCC 35640. (2E)-Dodecenal (C(12)) was the most effective against this food-borne bacterium with the minimum bactericidal concentration (MBC) of 6.25 microg/mL (34 microM), followed by (2E)-undecenal (C(11)) with an MBC of 12.5 microg/mL (74 microM). The time-kill curve study showed that these alpha,beta-unsaturated aldehydes are bactericidal against S. choleraesuis at any growth stage and that their bactericidal action comes in part from the ability to act as nonionic surfactants.  相似文献