Functional insulin‐like factor 3 (INSL3) hormone‐receptor system in the testes and spermatozoa of domestic ruminants and its potential as a predictor of sire fertility          下载免费PDF全文

Ali M. Pitia  Kyoko Uchiyama  Hiroaki Sano  Masashi Kinukawa  Yoshiaki Minato  Hiroshi Sasada  Tetsuya Kohsaka 《Animal Science Journal》2017,88(4):678-690

Insulin‐like factor 3 (INSL3) is essential for fetal testis descent, and has been implicated in the testicular and sperm functions in adult males; however, similar functions in domestic ruminants remain largely unknown. This study investigated the functional INSL3 hormone‐receptor system in adult ruminant testes and spermatozoa, and explored its potential to diagnose the fertility of sires. Testes and spermatozoa were obtained from fertile bulls, rams and he‐goats, whereas subfertile testes and spermatozoa were obtained only from bulls. As expected, INSL3 was visualized in Leydig cells, while we clearly demonstrated that the functional receptor, relaxin family peptide receptor 2 (RXFP2), enabling INSL3 to bind was identified in testicular germ cells and in the sperm equatorial segment of bulls, rams and he‐goats. In comparison to fertile bulls, the percentage of INSL3‐ and RXFP2‐expressing cells and their expression levels per cell were significantly reduced in the testes of subfertile bulls. In addition, the population of INSL3‐binding spermatozoa was also significantly reduced in the semen of subfertile bulls. These results provide evidence for a functional INSL3 hormone‐receptor system operating in ruminant testes and spermatozoa, and its potential to predict subfertility in sires.  相似文献   

Effects of climate change on reduction of internal bond strength of particleboard subjected to various climatic conditions in Japan     

Hideaki Korai  Katsuhiro Nakao  Tetsuya Matsui  Ken Watanabe  Yasushi Ishigooka 《Journal of Wood Science》2017,63(3):253-262

The effects of climate change on the reduction of internal bond strength (IB) of particleboard subjected to various climatic conditions in Japan were predicted. The temperature increased throughout Japan with increasing greenhouse gas (GHG) emissions, so the climate deterioration index (climate index for predicting the IB reduction of particleboard; a high index indicates a large IB reduction) also increased to decrease IB. The number of low-temperature areas decreased because of climate change. The rupture of bonding points in particleboard caused by outdoor exposure led to a IB reduction, and biodeterioration accelerated this reduction. In high-temperature areas, particleboard was found to be prone to biodeterioration, and the IB significantly reduced. An area wherein there was a significant decrease in the IB spread along the Pacific Ocean side of western Japan and the Sea of Japan in central Japan in relation to increasing GHG emissions. Particleboards are difficult to use outdoors because of climate change.  相似文献   

Novel reovirus isolation from an Ostrich (Struthio camelus) in Japan     

Sakai K  Ueno Y  Ueda S  Yada K  Fukushi S  Saijo M  Kurane I  Mutoh K  Yoshioka K  Nakamura M  Takehara K  Morikawa S  Mizutani T 《Veterinary microbiology》2009,134(3-4):227-232

An orthoreovirus was isolated from an Ostrich (Struthio camelus) and rapidly identified as orthoreovirus by the rapid determination of viral RNA sequences (RDV) system and electron microscopy. Phylogenetic analysis of the sigma A protein indicated that the isolate belonged to avian species and was closely related to chicken orthoreovirus strain 138. The results of the present study indicated that an ostrich orthoreovirus is slight different from other chicken orthoreoviruses and provided evidence of diversity among avian orthoreoviruses. To our knowledge, this is the first genetic report of an orthoreovirus isolated from an ostrich.  相似文献