Protective effect of oral administration of transgenic tobacco seeds against verocytotoxic Escherichia coli strain in piglets     

Luciana Rossi  Vittorio Dell’Orto  Simona Vagni  Vittorio Sala  Serena Reggi  Antonella Baldi 《Veterinary research communications》2014,38(1):39-49

The use of transgenic plants as delivery system for antigenic proteins is attractive for its simplicity and increases likelihood for local immune response at sites of infection. The aim of this study was to evaluate the protective effect of oral administration of tobacco seeds, expressing the FedA, the major protein of the F18 adhesive fimbriae, and B subunit of verocytotoxin, against verocytotoxin-producing E. coli (VTEC) strain in piglets. Forty-three early weaned piglets, were randomly divided into 4 experimental groups: 3 test groups and a control. Treatment groups orally received a bolus, with different dose of tobacco seeds on 0, 1, 2, 14 days post primary administration. After challenge, with 1*10¹⁰ CFU of O138 Escherichia coli strain, piglets showed clinical scores significantly higher in the control group compared to orally immunized groups (P?<?0.05) and the latter showed a faster recovery than in CG. In conclusion, oral administration of recombinant tobacco seeds expressing antigenic proteins against VTEC strains can induce a protective effect against challenger strain in piglets.  相似文献   

Parameters of the Reproductive Tract,Spermatogenesis, Daily Sperm Production and Major Seminal Plasma Proteins of Tropically Adapted Morada Nova Rams     

FML Sousa  CH Lobo  ESB Menezes  JPA Rego  RV Oliveira  AC Lima‐Souza  M Fioramonte  FC Gozzo  RCFF Pompeu  MJD Cândido  JT Oliveira  AA Moura 《Reproduction in domestic animals》2014,49(3):409-419

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A classical nova,V2487 Oph 1998, seen in x-rays before and after its explosion     

Hernanz M  Sala G 《Science (New York, N.Y.)》2002,298(5592):393-395

Classical nova explosions are very energetic and frequent phenomena caused by explosive hydrogen burning on top of an accreting white dwarf. Observations of the recent nova V2487 Oph 1998 by the X-ray Multi-Mirror satellite (XMM-Newton) provide evidence that accretion (probably on a magnetic white dwarf) was reestablished as early as 2.7 years after the explosion. In addition, positional correlation with a source previously discovered by the R?ntgen Satellite (ROSAT) in 1990 suggests that the site of a nova explosion had been seen in x-rays before the outburst.  相似文献   

Effect of the anion citrate on the mineral composition of artificial casein micelles     

Gatti CA  Alvarez EM  Sala VS 《Journal of agricultural and food chemistry》1999,47(1):141-144

The composition of artificial casein micelles (ACM) prepared at constant concentration of caseins, calcium (Ca), and phosphate (P(i)) in media with different citrate (Cit) concentrations was studied. The incorporation of the different mineral and protein components to the ACM was conditioned by the Cit concentration. In our working condition, the ACM size remained almost constant for Cit concentration ranging from 7 to 10 mM. This behavior could be indicating that the action of Cit essentially consists of a regulation of the Ca activity. The molar ratio at which Ca and P(i) were incorporated to the ACM varied for different Cit concentrations. At decreasing pH, the Ca/P(i) molar ratios for the remanent ions in the ACM were dependent on the Cit concentration. These observations could be related to a certain kind of competition between Cit, micellar calcium phosphate (MCP), and other groups able to bind Ca in the ACM.  相似文献   

Quantification,Morphology and Ultrastructure of Preantral Follicles of Buffalo (Bubalus bubalis) Foetuses     

SSD Santos  MAP Ferreira  MYS Lima  RV Sampaio  MS Cordeiro  TVG Silva  NN Costa  MS Miranda  OM Ohashi 《Reproduction in domestic animals》2011,46(1):e17-e22

The objective of this study was to determine the number, morphology and ultrastructure of preantral ovarian follicles of buffalo (Bubalus bubalis) foetuses at different ages. Quantification revealed number of primordial, primary and secondary follicles of 48 857 ± 17 506, 26 000 ± 20 452, 18 428 ± 10 875 and 18 375 ± 19 690, 225 ± 349, 326 ± 288 at 12–34 cm and 35–60 cm crown rump length (CRL), respectively. Follicular diameter values were 28.9 (±3.4), 34.7 (±5.9) and 59.4 (±12.6) μm; oocyte diameters were 21.7 (±2.8), 24.3 (±3.4) and 33.0 (±7.7) μm, and the numbers of follicular cells in the follicle equatorial section were 7.1 (±1.4), 12.0 (±2.4) and 13.8 (±2.4) for primordial, primary and secondary follicles, respectively. The primordial follicle consisted of an oocyte surrounded by a layer of flattened follicular cells with a normally eccentric oocyte nucleus. Dispersed Golgi complex, smooth endoplasmic reticulum, rounded mitochondria and several lipid vesicles were observed in the cytoplasm and cell junctions between the follicle cell membranes and the oocyte. This work describes the number, morphometry and ultrastructure of preantral follicles of buffalo foetuses, concluding that folliculogenesis is established between 8 and 34 cm CRL and that follicle number varies individually and according to age and that further studies are needed in this species.  相似文献   

Teratogenic effects of the interaction acetylsalicylic acid (ASA) and ethanol: morphologic and morphometric evaluation of the lingual epithelium in rat fetuses     

Marinho SA  Sala MA  Lopes RA  de Moraes Grisi MF  Novaes AB  de Souza SL  Taba M 《Anatomia, histologia, embryologia》2007,36(1):14-18

The objective of the present work was to evaluate the teratogenic effects of the interaction between acetylsalicylic acid (ASA) and ethanol on the epithelium of the lingual mucosa in rat fetuses. On the 10th pregnancy day, a single intraperitoneal ethanol dose (2.96 g/kg body weight) (Group I), ASA (200 mg/kg body weight) (Group II) and ASA plus ethanol, in the same doses (Group III), or saline (Group IV - control), were administrated. The epithelial alterations were assessed by means of histological and morphometric methods, on posterior dorsal, anterior dorsal and ventral regions of the tongue. ASA reduced, in rat fetuses, the ethanol deleterious effects on nuclear size in the epithelial prickle cell of the lingual mucosa. On the other hand, ASA did not influence the effects of ethanol in both epithelial layers of the lingual mucosa, when the nuclear shape, cell volume or epithelial layers thickness were evaluated.  相似文献   

A case report of <Emphasis Type="Italic">Mycoplasma wenyonii</Emphasis> associated immune-mediated haemolytic anaemia in a dairy cow     

Nicola Gladden  Hayley Haining  Livia Henderson  Francesco Marchesi  Libby Graham  Michael McDonald  Fraser. R. Murdoch  Anna Bruguera Sala  Jayne Orr  Kathryn Ellis 《Irish veterinary journal》2015,69(1):1

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A Rare Case of Polyorchidism in a Cat with Four Intra‐abdominal Testes          下载免费PDF全文

J Roca‐Ferrer  E Rodríguez  GA Ramírez  C Moragas  M Sala 《Reproduction in domestic animals》2015,50(1):172-176

Polyorchidism is a rare congenital anomaly defined as the presence of more than two histologically proven testes. We report a case of a 9‐month‐old European cat with four intra‐abdominal testes. The diagnosis was performed by means of ultrasonography, intra‐operative examination and histological confirmation. The case reported here presents an extremely rare anomaly, as no previous studies in veterinary medicine have reported the presence of four testes. This case suggests that supernumerary testes should be included as differential diagnoses for intra‐abdominal masses.  相似文献   

Endophytic fungi associated with Mediterranean plants as a source of mycelium-bound lipases   总被引：3，自引：0，他引：3  

Torres M  Dolcet MM  Sala N  Canela R 《Journal of agricultural and food chemistry》2003,51(11):3328-3333

A screening of endophytic fungi isolated from Mediterranean plants rendered a mycelium-bound lipase from a strain of Rhizopus oryzae that catalyzed the esterification of fatty acids in isooctane. The influence of various factors (water content, temperature, and pH) on ester synthesis was investigated. Catalytic activity was inversely correlated with water content. This enzyme was active over the entire pH range studied, from pH 3 to pH 8, and activity was maximal at pH 4 and pH 7. The enzyme was thermostable, with maximal activity at 60 degrees C.  相似文献   

Effects of isolation and fishing on the marine ecosystems of Easter Island and Salas y Gómez,Chile     

Alan M. Friedlander  Enric Ballesteros  Jim Beets  Eric Berkenpas  Carlos F. Gaymer  Matthias Gorny  Enric Sala 《水产资源保护:海洋与淡水生态系统》2013,23(4):515-531