Effect of dietary arginine to lysine ratios on productive performance,meat quality,plasma and muscle metabolomics profile in fast-growing broiler chickens     

Marco Zampiga  Luca Laghi  Massimiliano Petracci  Chenglin Zhu  Adele Meluzzi  Sami Dridi  Federico Sirri 《畜牧与生物技术杂志(英文版)》2019,(1)

Background: Due to the important functions of arginine in poultry,it should be questioned whether the currently adopted dietary Arg:Lys ratios are sufficient to meet the modern broiler requirement in arginine.The present study aimed,therefore,to evaluate the effects of the dietary supplementation of L-arginine in a commercial broiler diet on productive performance,breast meat quality attributes,incidence and severity of breast muscle myopathies and foot pad dermatitis(FPD),and plasma and muscle metabolomics profile in fast-growing broilers.Results: A total of 1,170 1-day-old Ross 308 male chicks was divided into two experimental groups of 9 replicates each fed either a commercial basal diet(CON,digestible Arg:Lys ratio of 1.05,1.05,1.06 and 1.07 in each feeding phase,respectively) or the same basal diet supplemented on-top with crystalline L-arginine(ARG,digestible Arg:Lys ratio of 1.15,1.15,1.16 and 1.17,respectively).Productive parameters were determined at the end of each feeding phase(12,22,33,43 d).At slaughter(43 d),incidence and severity of FPD and breast myopathies were assessed,while plasma and breast muscle samples were collected and analyzed by proton nuclear magnetic resonancespectroscopy.The dietary supplementation of arginine significantly reduced cumulative feed conversion ratio compared to the control diet at 12 d(1.352 vs.1.401,P 0.05),22 d(1.398 vs.1.420; P 0.01) and 33 d(1.494 vs.1.524; P 0.05),and also tended to improve it in the overall period of trial(1.646 vs.1.675; P = 0.09).Body weight was significantly increased in ARG compared to CON group at 33 d(1,884 vs.1,829 g; P 0.05).No significant effect was observed on meat quality attributes,breast myopathies and FPD occurrence.ARG birds showed significantly higher plasma concentration of arginine and leucine,and lower of acetoacetate,glutamate,adenosine and proline.Arginine and acetate concentrations were higher,whereas acetone and inosine levels were lower in the breast of ARG birds(P 0.05).Conclusions: Taken together,these data showed that increased digestible Arg:Lys ratio had positive effects on feed efficiency in broiler chickens probably via modulation of metabolites that play key roles in energy and protein metabolism.  相似文献   

Optimization of lipase-catalyzed synthesis of acetylated tyrosol by response surface methodology     

Aissa I  Bouaziz M  Ghamgui H  Kamoun A  Miled N  Sayadi S  Gargouri Y 《Journal of agricultural and food chemistry》2007,55(25):10298-10305

The ability of a noncommercial immobilized lipase from Staphylococcus xylosus (SXLi) to catalyze the transesterification of tyrosol and ethyl acetate was investigated. Response surface methodology was used to evaluate the effects of the temperature (40-60 degrees C), the enzyme amount (50-500 UI), and the ethyl acetate/hexane volume ratio (0.2-1) on the tyrosol acetylation conversion yield. Two independent replicates were carried out under the optimal conditions predicted by the model (reaction temperature 54 degrees C, enzyme amount 500 UI, and volume ratio ethyl acetate/hexane 0.2). The maximum conversion yield reached 95.36 +/- 3.6%, which agreed with the expected value (96.8 +/- 3.7%). The ester obtained was characterized by spectroscopic methods. Chemical acetylation of tyrosol was performed, and the products were separated using HPLC. Among the eluted products from HPLC, mono- and diacetylated derivatives were identified by positive mass spectrometry. Tyrosol and its monoacetylated derivative exert similar antiradicalar activity on 2,2-diphenyl-1-picrylhydrazyle.  相似文献   

Dissolution of wood in ionic liquids   总被引：10，自引：0，他引：10  

Kilpeläinen I  Xie H  King A  Granstrom M  Heikkinen S  Argyropoulos DS 《Journal of agricultural and food chemistry》2007,55(22):9142-9148

The present paper demonstrates that both hardwoods and softwoods are readily soluble in various imidazolium-based ionic liquids (ILs) under gentle conditions. More specifically, a variety of ionic liquids can only partially dissolve wood chips, whereas ionic liquids such as 1-butyl-3-methylimidazolium chloride and 1-allyl-3-methylimidazolium chloride have good solvating power for Norway spruce sawdust and Norway spruce and Southern pine thermomechanical pulp (TMP) fibers. Despite the fact that the obtained solutions were not fully clear, these ionic liquids provided solutions which permitted the complete acetylation of the wood. Alternatively, transparent amber solutions of wood could be obtained when the dissolution of the same lignocellulosic samples was attempted in 1-benzyl-3-methylimidazolium chloride. This realization was based on a designed augmented interaction of the aromatic character of the cation of the ionic liquid with the lignin in the wood. After dissolution, wood can be regenerated as an amorphous mixture of its original components. The cellulose of the regenerated wood can be efficiently digested to glucose by a cellulase enzymatic hydrolysis treatment. Furthermore, completely acetylated wood was found to be readily soluble in chloroform, allowing, for the first time, detailed proton nuclear magnetic resonance (NMR) spectra and NMR diffusion measurements to be made. It was thus demonstrated that the dissolution of wood in ionic liquids now offers a variety of new possibilities for its structural and macromolecular characterization, without the prior isolation of its individual components. Furthermore, considering the relatively wide solubility and compatibility of ionic liquids with many organic or inorganic functional chemicals or polymers, it is envisaged that this research could create a variety of new strategies for converting abundant woody biomass to valuable biofuels, chemicals, and novel functional composite biomaterials.  相似文献   

Lack of inhibitory effect of acetylsalicylic acid and meloxicam on whole blood platelet aggregation in cats     

Curtis J. Cathcart DVM  Benjamin M. Brainard VMD  DACVA  DACVECC  Lisa R. Reynolds BSc  Sami Al‐Nadaf BSc  Steven C. Budsberg DVM  MS  DACVS 《Journal of Veterinary Emergency and Critical Care》2012,22(1):99-106

  相似文献   

Seroprevalence of hypodermosis in cattle in some provinces of Turkey   总被引：1，自引：0，他引：1  

Simsek S  Utuk AE  Koroglu E  Dumanli N 《Research in veterinary science》2008,84(2):246-249

The aim of this study was to determine the seroprevalence of hypodermosis in cattle in the east and southeast of Turkey. For this purpose, a total of 634 sera samples of cattle were collected from Malatya, Elazig and Diyarbakir provinces of east and southeast of Turkey from November 2005 to February 2006. The sera were analyzed using a Hypodermin C antigen by means of indirect ELISA. One hundred and forty eight (23.3%) out of 634 cattle were seropositive for hypoderma antibodies. The highest percentage of seropositivity were detected at Elazig province (26.3%) followed by Malatya (22.3%) and Diyarbakir provinces (22.1%). The seropositivity rate was higher in female (31%) than male (14.1%). When the mean is considered by animal breed, the highest seropositivity was detected at local breed (27.7%) following crossbreed (26.8%) and purebreed (19.7%). There was a positive relation between age and seropositivity. Seropositivity rate was 15.9% in 2 and under ages while these rates were 38.1% and 30.4% in 3-4 ages and 5 and up ages, respectively.  相似文献   

Assessments of first feeding protocols on the larviculture of California grunion Leuresthes tenuis (Osteichthyes: Atherinopsidae)     

Yen‐Ju Pan  Emilien Dpos  Anissa Souissi  Stphane Hnard  Mike Schaadt  Ed Mastro  Sami Souissi 《Aquaculture Research》2020,51(7):3054-3058

  相似文献   

Combining eight research areas to foster the uptake of ecosystem‐based management in fresh waters     

Simone D. Langhans  Sami Domisch  Stefano Balbi  Gonzalo Delacmara  Virgilio Hermoso  Mathias Kuemmerlen  Romina Martin  Javier Martínez‐Lpez  Peter Vermeiren  Ferdinando Villa  Sonja C. Jhnig 《水产资源保护:海洋与淡水生态系统》2019,29(7):1161-1173

Characterization of difenoconazole resistance in Lasiodiplodia theobromae from papaya in Brazil     

Yuan Li  Susan Satie Tsuji  Mengjun Hu  Marcos Paz Saraiva Cmara  Sami Jorge Michereff  Guido Schnabel  Fengping Chen 《Pest management science》2020,76(4):1344-1352

  相似文献   

Prevalence and antimicrobial resistance of Campylobacter coli isolated from fattening pigs in France     

Payot S  Dridi S  Laroche M  Federighi M  Magras C 《Veterinary microbiology》2004,101(2):91-99

Campylobacter are a leading cause of human diarrhea. The usual source of infection is contaminated food, particularly poultry but pork has also been described. The veterinary use of antimicrobial drugs has been suggested to be largely responsible for resistance in human isolates of this zoonotic pathogen. A study was carried out to investigate the occurrence and antimicrobial resistance of Campylobacter isolated from French fattening pigs. From March 1998 to June 1999, stomach samples were collected at slaughter from 240 fattening pigs originating from 24 different farms. Half of the pigs were found to be positive for Campylobacter but considerable variation was observed between farms. Isolates all belong to the Campylobacter coli species. Susceptibilities of the strains were determined for five antimicrobial drugs using agar dilution. Resistance to tetracycline and erythromycin was high (79 and 55%, respectively). For nalidixic acid, enrofloxacin and ampicillin, resistance was observed in 34, 15 and 20% of the isolates, respectively. More than one-third of the strains was resistant to at least three antimicrobial drugs. A Thr86Ile modification in GyrA was observed in the enrofloxacin-resistant strains studied. The multiresistant strains analyzed expressed the multidrug transporter CmeB at a high level. Results indicated a high prevalence of C. coli in the stomach of the French pigs examined. In addition, a high proportion of the strains was resistant to antimicrobial drugs, particularly to tetracycline and erythromycin, or were multiresistant.  相似文献   

Production of interspecific F1 hybrids, BC1, BC2 and BC3 populations between Lycopersicon esculentum and two accessions of Lycopersicon peruvianum carrying new root-knot nematode resistance genes     

Sami Doganlar  Anne Frary  Steven D. Tanksley 《Euphytica》1997,95(2):203-207

Two accessions of Lycopersicon peruvianum Mill. (PI270435, PI126443) carrying Mi-2 and Mi-3, respectively, new root-knot nematode resistance genes, were selected as the male parents for crosses with L. esculentum Mill. in order to produce interspecific hybrids. Crossability barriers between these two distantly related species were circumvented by ovule culture. A total of ten interspecific F₁ hybrid plants were produced. The hybrid nature of the putative F₁ plants was confirmed by a comparison of several morphological characteristics and a PCR-based assay. Eight of ten hybrid plants were backcrossed with L. esculentum to generate a total of 98 BC₁ progeny. Two lines were advanced to the BC₂ and BC₃ levels. Based on these results, ovule culture was found to be an effective method for the production of novel interspecific F₁ and BC₁ plants. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献