Dynamic interfacial rheology as a tool for the characterization of whey protein isolates gelation at the oil-water interface.     

J M Rodriguez Patino  M R Rodriguez Nino  C Carrera Sanchez 《Journal of agricultural and food chemistry》1999,47(9):3640-3648

Heat-induced interfacial aggregation of a whey protein isolate (WPI), previously adsorbed at the oil-water interface, was studied by interfacial dynamic characteristics coupled with microscopic observation and image analysis of the drop after heat treatment. The experiments were carried out at temperatures ranging from 20 to 80 degrees C with different thermal regimes. During the heating period, competition exists between the effect of temperature on the film fluidity and the increase in mechanical properties associated with the interfacial gelation process. During the isothermal treatment, the surface dilational modulus, E, increases, and the phase angle, delta, decreases with time to a plateau value. The frequency dependence of E and delta is characteristic of viscoelastic films with increasing delta and decreasing E at lower frequencies. The effects of heat treatment depend on the conditions at which the gelation process takes place. Microscopic observation of gelled films gives complementary information on the effect of heat treatment on WPI adsorbed films.  相似文献   

Disseminated nocardiosis associated with the isolation of Nocardia nova in a longsnout seahorse Hippocampus reidi (Ginsburg)          下载免费PDF全文

J Dill  S Sanchez  A McDermott  A Camus 《Journal of fish diseases》2017,40(9):1235-1239

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In Vitro and In Vivo Feedstuff Digestibility for Snook,Centropomus undecimalis,Juveniles          下载免费PDF全文

Iratzio Lemus  Carlos Maldonado  Gerard Cuzon  Adolfo Sanchez  Gabriela Gaxiola  Alfonso Alvarez  Martha Guerrero 《Journal of the World Aquaculture Society》2018,49(1):205-215

The degree of hydrolysis (DH) of the ingredients was evaluated for Centropomus undecimalis juveniles. The in vitro experiment included wheat gluten (WG), corn gluten (CG), soybean meal (SBM) and pretreated phytase (SBM + phytase), soy protein concentrate (SPC), canola meal (CAN) and pretreated phytase (CAN + phytase), poultry byproduct meal (PBPM), chicken meal (CHKM), dried whey (DW), Pota meal (PM: mix of giant squid, fish soluble protein concentrate, crustacean meal, and fish oil), and Protiblend (PTB: aquatic and render mix). The highest acidic DH occurred with PTB (0.38 ± 0.06), CHKM (0.33 ± 0.3), and PBPM (0.25 ± 0.03). In the alkaline condition, PTB (1.6 ± 0.17 and 0.98 ± 0.05 for pyloric caeca and intestine, respectively) and CG (1.04 ± 0.4 and 0.75 ± 0.2 for caeca and intestine, respectively) provided the highest DH values. In vitro digestibility demonstrated that PTB was the highest (85.3%) followed by PBPM (51.4%), CAN + phytase (47.6%), CG (45.1%), and CHKM (46.5%). The in vivo experiment concerned the WG, CHKM, PBPM, PM, and PTB diets plus a reference diet with fishmeal (Ref diet). The total %DH was different (P < 0.05) with the lowest values for the WG diet (0.34 ± 0.09%) and Ref diet (0.34 ± 0.15). Free amino acid released during digestion was displayed for these diets and a bifactorial analysis produced no difference (P > 0.05). The apparent digestive coefficients ranged from 89.8 to 92.9% for protein and from 68 to 71.4% for energy.  相似文献   

Mapping additional QTLs from FR13A to increase submergence tolerance in rice beyond SUB1     

Zennia Jean C. Gonzaga  Jerome Carandang  Darlene L. Sanchez  David J. Mackill  Endang M. Septiningsih 《Euphytica》2016,207(3):627-643

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New insights into radiata pine seedling root infection by Fusarium circinatum          下载免费PDF全文

N. Martín‐Rodrigues  J. Sanchez‐Zabala  I. Salcedo  J. Majada  C. González‐Murua  M. K. Duñabeitia 《Plant pathology》2015,64(6):1336-1348

Pine root infection by Fusarium circinatum has been reported in the literature, but the underlying pathogenic interaction is poorly understood. A green fluorescent protein (GFP)‐tagged F. circinatum isolate, together with confocal microscopy, was used in order to monitor the events associated with root infection of Pinus radiata seedlings. It was found that in order to reach and successfully infect pine roots, F. circinatum employed features that are similar to those previously described for other root‐infecting pathogens, such as mycelial strands, single runner hyphae and simple hyphopodia as well as other features that are reminiscent of those that are known to be involved in biotrophic invasion, such as bulbous invasive hyphae and filamentous invasive hyphae. Abundant sporulation was observed at the root surface as well as inside tracheids both in roots and in the root collar region. The fungus can spread from the roots to the aerial parts of the plant, and once there, colonization appears to be similar to the process that occurs when the pathogen is inoculated in the stem. Wilting symptoms and plant demise may be the result of a reduction in water uptake by roots and of the blockage of the vascular system by fungal hyphae and resin.  相似文献   

The potential of pet‐grade poultry by‐product meal to replace fish meal in the diet of the juvenile spotted rose snapper Lutjanus guttatus (Steindachner, 1869)     

C. Hernández  Y. Sanchez‐Gutierrez  R. W. Hardy  A. Benitez‐Hernández  P. Domínguez‐Jimenez  B. González‐Rodríguez  L. Osuna‐Osuna  O. Tortoledo 《Aquaculture Nutrition》2014,20(6):623-631

A 12‐week feeding trial was conducted to examine the replacement of fish meal with pet‐grade poultry by‐product meal (PBM‐PG) in the spotted rose snapper Lutjanus guttatus diet. Five experimental diets were formulated to contain graded levels of PBM‐PG at proportion of 250, 500, 75 or 900 g kg^?1. The control diet contained sardine fish meal as the main protein source. Four groups of 15 randomly assigned L. guttatus juveniles were fed to satiation 3 times day^?1. Except for the fish fed the PBM‐PG90 diet, the growth performance, survival and feed utilization efficiency of the experimental fish were not significantly lower than those of the control fish. The dietary level of PBM‐PG did significantly affect the haematological characteristics (P < 0.05). The dietary dry matter and protein apparent digestibility coefficients (ADCs) decreased with increasing dietary PBM‐PG. High values for lipid ADCs were observed in all diets, with significant differences among the dietary treatments. The fish whole‐body protein, moisture, lipid and ash contents were not affected by the inclusion of dietary PBM. These results indicate that high‐quality terrestrial PBM can successfully replace more than half of the marine fish meal protein in the L. guttatus diet.  相似文献   

Fertilizing Capacity of Frozen Epididymal Sperm Collected from Dogs     

MIM Martins  LC Padilha  FF Souza  MD Lopes 《Reproduction in domestic animals》2009,44(S2):342-344

The collection of epididymal sperm may be a valuable tool for canine reproduction especially since it can enable collection of cells after death of a valuable dog. The aim of the present study was to evaluate the viability of epididymal sperm after freeze-thawing. Epididymides were obtained from four adult dogs by elective orchiectomy. The caudal portion of the epididymides and part of the deferential ducts were squeezed by means of an anatomic clamp into a Petri dish containing either 0.9% saline solution (Group 1) or Ringer solution without lactate (Group 2). Samples were centrifuged at 800 ×  g for 10 min, the supernatant was removed and the pellet was diluted in one step with a Tris/citric acid/OEP (Orvus Es Paste) extender containing 7% glycerol and subjected to semen freezing. Oocytes were obtained from canine ovaries, after ovariohysterectomy. Only oocytes that were approximately 100 μm in diameter, with a dark ooplasm surrounded by three- or four-well formed cumulus cell layers were used for sperm testing. Frozen semen samples were thawed in a water bath at 70°C for 8 s and analysed at room temperature for sperm motility and velocity. Oocytes were incubated with spermatozoa in humidified atmosphere containing 5% CO₂ at 38°C for 18 h. Morphological and functional characteristics of spermatozoa were similar in both groups. However, the percentage of sperm cells bound to oocytes was significantly higher in Group 2 than in Group 1. This result suggests that the Ringer solution without lactate was a more suitable medium for collecting epididymal canine sperm than 0.9% saline.  相似文献   

The effect of repeated eye examinations and breeding advice on the prevalence and incidence of cataracts and progressive retinal atrophy in German dachshunds over a 13‐year period          下载免费PDF全文

Sarah Koll  Sven Reese  Ivica Medugorac  Carsten U. Rosenhagen  Rick F. Sanchez  Roberto Köstlin 《Veterinary ophthalmology》2017,20(2):114-122

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Panniculitis attributable to Mycobacterium goodii in an immunocompetent dog in Georgia     

Krimer PM  Phillips KM  Miller DM  Sanchez S 《Journal of the American Veterinary Medical Association》2010,237(9):1056-1059

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Reference values of respiratory and peripheral muscle function in rats     

E. Barreiro  J. Marín‐Corral  F. Sanchez  V. Mielgo  F. J. Alvarez  J. B. Gáldiz  J. Gea 《Journal of animal physiology and animal nutrition》2010,94(6):e393-e401

Skeletal muscle dysfunction is a common systemic manifestation in several prevalent diseases. Predictive values are useful tools for the diagnosis and prognosis of diseases. In experimental animals, no reference values of muscle function evaluation have been so far reported. The objective was to obtain predictive values of maximal inspiratory pressure (MIP) and grip strength measurements in healthy rats. In 70 healthy rats, MIP and grip strength were measured in vivo weekly for five consecutive weeks using non‐invasive methodologies. Three ranges of rat body weights (250–299, 300–349 and 350–399 g) and lengths (37.0–41.0, 41.1–42.0 and 42.1–44.0 cm) were established. MIP and grip strength measurements falling within the ranges of weight 350–399 and 300–349 g and length 42.1–44.0 cm were significantly greater than values falling within 250–299 g and 37.0–41.0 cm ranges respectively. Specific weight‐ and length‐percentile distributions for MIP and grip strength measurements were calculated. As significant direct correlations were observed between rat weights and lengths and either MIP or grip strength measurements, regression equations relating all these variables were also determined. Skeletal muscle dysfunction is frequently associated with highly prevalent conditions. The significant predictive equations described for both MIP and grip strength measurements will enable scientists to better estimate the respiratory and peripheral muscle dysfunctions of laboratory animals, especially when conducting follow‐up and/or intervention investigations.  相似文献