Dietary Protein Hydrolysates from Animal By-Products: Digestibility and Enzymatic Activity for Dourado Salminus brasiliensis     

Evandro Kleber Lorenz  Rafael Simões Coelho Barone  Fernando Yugo Yamamoto 《Journal Of Aquatic Food Product Technology》2018,27(2):236-246

Fishmeal (FM) is a high-cost and scarce feedstuff. Notwithstanding, the formulation and processing of diets for carnivorous fish strongly rely on FM as a protein source, given its palatability and biological value. Animal by-product hydrolysates are high-quality feedstuff and can substitute FM in aquafeeds. This study evaluated the digestibility of hydrolysates from tilapia residue (TR), tuna head (TH), swine liver (SL), and poultry liver (PL), and the profile of digestive enzymes in juvenile dourado, Salminus brasiliensis, a carnivorous Characin, fed diets containing graded levels of the hydrolysates. Inclusion of hydrolysates in diet formulations lowered the pH of feed but did not alter feed intake by fish. Higher apparent digestibility coefficients (ADCs) of diets were recorded for fish fed diets containing TR and SL, and lower ADCs were recorded for fish fed diets containing TH. Protease and lipase activities in the animal’s stomach were higher, especially for those fed with diets containing SL. Amylase activity was higher in pyloric caeca, whereas in the intestines, the higher activity was seen in fish fed control and TR diets. Animal by-product hydrolysates were highly digestible for dourado, and the enzymatic profile of fish was influenced by the nutrient contents of diets.  相似文献   

Lippia alba (Verbenaceae) hydrolate as sedative of tambaqui (Colossoma macropomum) juveniles in simulated transport conditions          下载免费PDF全文

Hugo Napoleão Pereira da Silva  Elen Monique de Oliveira Sousa  Janna Laely dos Santos Maia  Mônica Tatiane Lima Pinheiro  Soraia Valeria de Oliveira Coelho Lameirão  Rosa Helena Veras Mourão  José Guilherme Soares Maia  Bernardo Baldisserotto  Lenise Vargas Flores da Silva 《Aquaculture Research》2018,49(1):128-134

Lippia alba hydrolate (LAH) is produced as a secondary product in essential oil extraction. The objective of this study was to evaluate the use of LAH in a simulation of the transport of tambaqui (Colossoma macropomum) juveniles. A series of 17 hr simulations were performed using load densities of 30, 60 and 90 juveniles per L and four LAH concentrations of 0.4%, 1%, 2% and 5%, with a control of just water. Fish survival, temperature, dissolved oxygen, conductivity, pH, ammonia excretion, alkalinity water hardness and net Na⁺, K⁺, Cl^‐ fluxes, were all evaluated before and after the transport simulations. Mucous cell densities in the gills were also determined microscopically, post simulation. The results showed that addition of 5% LAH achieved better survival during the simulation than the water control. Dissolved oxygen was significantly lower with an increased load density. Also, conductivity, NH₄ excretion and net ionic fluxes (K⁺ and Cl^?) were lower with 5% LAH, although mainly where higher load densities were tested. Mucous cell density was lower in LAH concentrations of 2% and 5%, again with higher load densities. In view of these findings, it is suggested that 5% LAH is an effective sedative for use in the transport of tambaqui, mainly with high load densities. This work is the first study of LAH and highlights its potential applications in aquaculture management.  相似文献   

Recirculation nursery systems for bivalves     

Pauline Kamermans  Ainhoa Blanco  Sandra Joaquim  Domitília Matias  Thorolf Magnesen  Jean Louis Nicolas  Bruno Petton  Rene Robert 《Aquaculture International》2016,24(3):827-842

In order to increase production of bivalves in hatcheries and nurseries, the development of new technology and its integration into commercial bivalve hatcheries is important. Recirculation aquaculture systems (RASs) have several advantages: high densities of the species can be cultured resulting in a cost-effective production system; optimal temperature maximizes production and allows rapid turnover of the product; stable water quality improves growth rate and minimizes stress and potential loss by diseases. Pilot RAS systems were developed for seed rearing of oysters (Crassostrea gigas), scallops (Pecten maximus), mussels (Mytilus edulis) and clams (Ruditapes decussatus). Optimal feed addition and waste matrix were determined. Based on this, system flow rates were designed. Seed growth in the pilot RAS systems was compared at different renewal rates and with growth in flow-through systems (FTS). All four species can be reared in RAS and showed similar growth in RAS and in FTS or in RAS with a higher renewal rate. RAS can keep O₂, nitrogen and pH within the desired range. Temperature was generally higher in RAS than in FTS, probably due to heat induced by the pump circulating the water. The supply of sufficient amount of food in combination with a desire to reduce the renewal rate calls for use of concentrated feed in RAS.  相似文献   

Responses of the two‐spotted oak buprestid,Agrilus biguttatus (Coleoptera: Buprestidae), to host tree volatiles          下载免费PDF全文

József Vuts  Christine M Woodcock  Mary E Sumner  John C Caulfield  Katy Reed  Daegan JG Inward  Simon R Leather  John A Pickett  Michael A Birkett  Sandra Denman 《Pest management science》2016,72(4):845-851

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Dissolved and colloidal phosphorus fluxes in forest ecosystems—an almost blind spot in ecosystem research          下载免费PDF全文

Roland Bol  Dorit Julich  Dominik Brödlin  Jan Siemens  Klaus Kaiser  Michaela Anna Dippold  Sandra Spielvogel  Thomas Zilla  Daniela Mewes  Friedhelm von Blanckenburg  Heike Puhlmann  Stefan Holzmann  Markus Weiler  Wulf Amelung  Friederike Lang  Yakov Kuzyakov  Karl‐Heinz Feger  Nina Gottselig  Erwin Klumpp  Anna Missong  Carola Winkelmann  David Uhlig  Jakob Sohrt  Klaus von Wilpert  Bei Wu  Frank Hagedorn 《植物养料与土壤学杂志》2016,179(4):425-438

Understanding and quantification of phosphorus (P) fluxes are key requirements for predictions of future forest ecosystems changes as well as for transferring lessons learned from natural ecosystems to croplands and plantations. This review summarizes and evaluates the recent knowledge on mechanisms, magnitude, and relevance by which dissolved and colloidal inorganic and organic P forms can be translocated within or exported from forest ecosystems. Attention is paid to hydrological pathways of P losses at the soil profile and landscape scales, and the subsequent influence of P on aquatic ecosystems. New (unpublished) data from the German Priority Program 1685 “Ecosystem Nutrition: Forest Strategies for limited Phosphorus Resources” were added to provide up‐to‐date flux‐based information. Nitrogen (N) additions increase the release of water‐transportable P forms. Most P found in percolates and pore waters belongs to the so‐called dissolved organic P (DOP) fractions, rich in orthophosphate‐monoesters and also containing some orthophosphate‐diesters. Total solution P concentrations range from ca. 1 to 400 µg P L^?1, with large variations among forest stands. Recent sophisticated analyses revealed that large portions of the DOP in forest stream water can comprise natural nanoparticles and fine colloids which under extreme conditions may account for 40–100% of the P losses. Their translocation within preferential flow passes may be rapid, mediated by storm events. The potential total P loss through leaching into subsoils and with streams was found to be less than 50 mg P m^?2 a^?1, suggesting effects on ecosystems at centennial to millennium scale. All current data are based on selected snapshots only. Quantitative measurements of P fluxes in temperate forest systems are nearly absent in the literature, probably due to main research focus on the C and N cycles. Therefore, we lack complete ecosystem‐based assessments of dissolved and colloidal P fluxes within and from temperate forest systems.  相似文献   

Influence of stage of development in the efficiency of nitrogen fertilization on poplar     

Berta Lasa  Ivan Jauregui  Iker Aranjuelo  Sandra Sakalauskiene  Pedro M. Aparicio-Tejo 《Journal of plant nutrition》2016,39(1):87-98

Poplar cuttings (Populus x euroamericana) were planted in pots and grown in a greenhouse for four months with drip irrigation. Two nitrogen (N) doses of 60 and 120 kg nitrate (N-NO₃^?) ha^?1 (labeling with ¹⁵N stable isotopes) were applied at one (time I), two (time II) and three months after of the plantation (time III). The aim of this study was to determine the N-fertilizer application method that resulted in optimal N-uptake by a short-rotation of poplar crop during first year of growth. Our data revealed that NUE (N use efficiency) was largest in the last part of the experiment. The larger development of those plants at this stage enabled the improved absorption and assimilation of N. Furthermore, ¹⁵N also revealed that supply of N at three months post-planting generated more biomass and increased the N reserves in the stem; a key factor for ensuring the optimum regrowth during the second year.  相似文献   

Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation     

Hilton Marcelo de Lima Souza  Lara Durães Sette  Adolfo José da Mota  Joaquim Ferreira do Nascimento Neto  André Rodrigues  Tássio Brito de Oliveira  Fernando Mendes de Oliveira  Luiz Antônio de Oliveira  Hileia dos Santos Barroso  Sandra Patricia Zanotto 《Water, air, and soil pollution》2016,227(12):431

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Bait station devices can improve mass trapping performance for the control of the Mediterranean fruit fly          下载免费PDF全文

Vicente Navarro‐Llopis  Jaime Primo  Sandra Vacas 《Pest management science》2015,71(7):923-927

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Methods for Cercospora coffeicola protoplast isolation and genetic transformation with the green fluorescent protein     

André Gomes Coelho Souza  Sonia Herrero  Luiz Antonio Maffia  Margaret Elizabeth Daub 《European journal of plant pathology / European Foundation for Plant Pathology》2014,139(2):235-238

Cercospora coffeicola is the causal agent of brown eye spot on coffee leaves. Although the disease has significant importance, few molecular studies have been done with C. coffeicola. Here we report a protocol for isolating protoplasts as well as development of a genetic transformation system using Green Fluorescent Protein. High yields of protoplasts (≈10⁸/ml) were obtained from mycelial cultures from five isolates of C. coffeicola. One isolate was transformed with a vector encoding hygromycin resistance and Green Fluorescent Protein. Out of 43 hygromycin-resistant transformants obtained, Green Fluorescent Protein was highly expressed in one (2.3 %).  相似文献   

Development and characterization of a new set of 164 polymorphic EST‐SSR markers for diversity and breeding studies in rubber tree (Hevea brasiliensis Müll. Arg.)     

Philippe Cubry  Valérie Pujade‐Renaud  Dominique Garcia  Sandra Espeout  Vincent Le Guen  Françoise Granet  Marc Seguin 《Plant Breeding》2014,133(3):419-426

Despite its economic importance and recent genome release, the need for molecular tools for Hevea brasiliensis is high. In the frame of a disease resistance study, EST sequences were retrieved from public database or generated by sequencing SSH libraries. Sequences were trimmed and microsatellite motifs searched using an ad hoc bioinformatic pipeline, and pairs of primers for the amplification of candidate markers were generated. We found a total of 10 499 unigenes from both sources of sequences, and 673 microsatellites motifs were detected using the default parameters of the pipeline. Two hundred sixty‐four primer pairs were tested and 226 (85.6%) successfully amplified. Out of the amplified candidate markers, 164 exhibited polymorphism. Relationships based on dendrograms using simple matching index and diversity statistics based on EST‐SSRs were compared with Genomic SSRs, showing the potentialities of EST‐derived microsatellites for resistance studies but also for population genetics approaches.  相似文献